The Isotropic Fractionator as a Tool for Quantitative Analysis in Central Nervous System Diseases

One major aim in quantitative and translational neuroscience is to achieve a precise and fast neuronal counting method to work on high throughput scale to obtain reliable results.Here we tested the Isotropic Fractionator (IF) method for evaluating neuronal and non-neuronal cell loss in different models of central nervous system (CNS) pathologies.Sprague-Dawley rats underwent: (i) ischemic brain damage; (ii) intraperitoneal injection with kainic acid (KA) to induce epileptic seizures; and (iii) monolateral striatal injection with quinolinic acid (QA) mimicking human Hungtington’s disease.All specimens were processed for IF method and cell loss assessed.Hippocampus from KA-treated rats and striatum from QA-treated rats were carefully dissected using a dissection microscope and a rat brain matrix. Ischemic rat brains slices were first processed for TTC staining and then for IF.In the ischemic group the cell loss corresponded to the neuronal loss suggesting that hypoxia primarily affects neurons. Combining IF with TTC staining we could correlate the volume of lesion to the neuronal loss; by IF, we could assess that neuronal loss also occurs contralaterally to the ischemic side.In the epileptic group we observed a reduction of neuronal cells in treated rats, but also evaluated the changes in the number of non-neuronal cells in response to the hippocampal damage

Pedotransference functions for prediction of density in soils of Piauí, Brazil / Funções de pedotransferência para predição da densidade em solos do Piauí, Brasil

The determination of the density of the horizons of a soil profile allows to evaluate certain properties, such as: porosity, hydraulic conductivity and water storage. However, measured data is not always available or easy to obtain. Thus, it was objective with the work to build and evaluate models of pedotransference function based on multiparameters of the soil as alternatives for the estimation of soil density in areas with agricultural potential of the state of Piauí. Thus, soil samples were collected from 42 soil profiles in the state of Piauí at depths of 0.0-0.20; 0.20-0.40; and 0.40-0.60 m e, characterized in terms of chemical and physical attributes. Through multiple regression analysis, two pedotransfer function models were generated: i) including all determined attributes and, ii) only the particle size analysis and total organic carbon. For both models, all classes (generalized character) were considered, as well as each individual soil class Latossolo (Ferralsol), Argissolo (Acrisol), Plintossolo (Plinthosol) and Neossolo (Arenosol). The generated pedotransference functions are alternative to estimate bulk density of soil, obtaining "excellent" performance in model I for the "Latossolos" (Ferralsols) and "Argissolos" (Acrisols), which included a greater number of variables in the construction of the predictive model of bulk density

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Acúmulo de forragem e estrutura dos dosséis durante o diferimento das cultivares Marandu, Piatã, Xaraés e Paiaguás de capim-braquiária

The objective of this work was to compare herbage accumulation and canopy structural characteristics during the stockpiling period of the Marandu, Piatã, Xaraés, and Paiaguás cultivars of Urochloa brizantha. The experimental design was completely randomized, with three replicates. The experiment was repeated for two years (2017 and 2018), under mechanical cutting conditions, and the canopies were stockpiled for 90 days, from March to June. In the first year, forage mass was higher for the Xaraés and Paiaguás cultivars; however, in the second year, Xaraés showed a forage mass higher than that of the other cultivars. The number of vegetative tillers at the end of the stockpiling period was higher for Paiaguás, contrary to what was observed for number of reproductive tillers. The herbage accumulation rate was higher for the Paiaguás and Xaraés cultivars. The Paiaguás grass stands out for its high herbage accumulation rate during the stockpiling period and for its higher number of vegetative tillers and lower number of reproductive tillers than that of the other cultivars.O objetivo deste trabalho foi comparar o acúmulo de forragem e as características estruturais de dosséis durante o período de diferimento das cultivares Marandu, Piatã, Xaraés e Paiaguás de Urochloa brizantha. O delineamento experimental foi inteiramente casualizado, com três repetições. O experimento foi repetido por dois anos (2017 e 2018), sob condição de corte mecânico, e o dossel foi diferido por 90 dias, de março a junho. No primeiro ano, a massa de forragem foi maior para as cultivares Xaraés e Paiaguás; porém, no segundo ano, Xaraés apresentou massa de forragem maior do que a das demais cultivares. O número de perfilhos vegetativos no final do período de diferimento foi maior para Paiaguás, contrariamente ao que foi observado para número de perfilhos reprodutivos. Já a taxa de acúmulo de forragem foi maior para as cultivares Paiaguás e Xaraés. O capim Paiaguás se destaca por sua alta taxa de acúmulo de forragem durante o período de diferimento e por seu maior número de perfilhos vegetativos e menor número de perfilhos reprodutivos do que os das outras cultivares

Magnetization transfer ratio as a predictor of malignancy in breast lesions: Preliminary results

MRI is an important tool for investigating breast cancer. Although recognized as the method of choice for screening highrisk patients, and for other indications the role of MRI for lesion characterization remains controversial. Recently some authors have advocated the use of morphologic and postcontrast features for this purpose. Quantitative breast MRI techniques have not been applied extensively in breast diseases. Magnetization transfer (MT) is a quantitative MR technique commonly used to investigate neurological diseases. In breast diseases the use of MT has been limited to improving visualization of areas of enhancement in postcontrast images. The purpose of this study was to evaluate the feasibility and utility of MT in discriminating benign from malignant breast lesions. Fifty-two lesions, Bl-RADS 4 and 5, from 49 patients, were prospectively evaluated using the MT ratio (MTR). Patients were divided into two groups: benign and malignant lesions. The MTR of fat, pectoralis major muscle, fibroglandular tissue, and breast lesions were calculated. A statistically significant difference was found between MTR from benign and malignant lesions (P < 0.001). Preliminary results suggest that MT can be used to evaluate breast lesions. Further studies are necessary to better define the utility and applicability of this technique

Breast ultrasound diagnostic performance and outcomes for mass lesions using Breast Imaging Reporting and Data System category 0 mammogram

PURPOSE: To evaluate the outcomes and diagnostic performance of ultrasonography after a Breast Imaging Reporting and Data System (Bi-RADS) category 0 mammogram. MATERIAL AND METHODS: This retrospective study reviewed 4,384 consecutive patients who underwent a screening mammography from January 2005 to July 2006; 391 of the 4,384 exams were classified as Bi-RADS category 0. After exclusions, 241 patients received subsequent sonogram. Ultrasonography was considered diagnostic when the Bi-RADS category was changed to 2, 4, or 5, and it was considered indeterminate (Bi-RADS 3) when the results indicated that the patients should return for a mammographic follow-up. The outcomes of these patients were assessed to evaluate the diagnostic performance of ultrasonography. RESULTS: The mean age of the patients was 53.3 years (ranging from 35 to 81). Of the 241 patients, ultrasonography was considered diagnostic in 146 (60.6%) patients and indeterminate in 95 (39.4%) patients. In the diagnostic group, 111 out of 146 patients (70.2%) had a sonogram result of Bi-RADS category 2 after a 2-year follow-up without evidence of malignancy. Furthermore, 35 out of 146 patients (29.8%) had a suspicious sonogram with a result of Bi-RADS category 4. After a tissue sampling procedure, 10 patients were confirmed to have breast cancer, and 25 had benign histopathological features without any evidence of malignancy after a 2-year follow-up. The sensitivity of ultrasonography was 100%, specificity was 89.1%, and overall accuracy was 89.6%. CONCLUSIONS: Based on the degree of resolution and its diagnostic performance, ultrasonography was determined to be an excellent method for the subsequent evaluation of Bi-RADS 0 mammograms