54 research outputs found
Bystander-Mediated Regression of Murine Neuroblastoma via Retroviral Transfer of the HSV-TK Gene
Selective introduction of genes conferring chemosensitivity into proliferating tumor cells may be used to treat cancer. We investigated the bystander effect of retrovirusmediated gene transfer of herpes simplex virus thymidine kinase (HSV-TK) gene to murine neuroblastoma cell line (neuro-2a) in vitro and in vivo, and we examined whether the mechanism of bystander effect in neuroblastoma would also depend on connexin-dependent gap junction and/or immune response. A strong bystander effect was observed in vitro, whereby nontransduced tumor cells in proximity to transduced cells acquired susceptibility to ganciclovir (GCV) killing. Implanted mixtures of wildtype cells and HSV-TK transduced cells showed a potent bystander effect upon administration of GCV in A/J mice. HSV-TK/GCV system in murine neuroblastoma induced systemic immunity. Immunohistochemical staining showed many CD4+ and CD8+ cell infiltration but did not show anti-connexin 43+ cells. In conclusion, a strong bystander effect was observed in vitro and in vivo. The bystander effect in murine neuroblastoma might be dependent on immune response and/or on other mechanism such as protein phosphorylation or transfer of apoptotic vesicle, rather than connexin-dependent gap junction
A systematic review of the effectiveness of interventions to improve post-fracture investigation and management of patients at risk of osteoporosis
ComposiçÔes microbicidas, processos para sua obtenção, construçÔes gĂȘnicas, processos para o controle de pestes
Em 17/03/2016: Anuidade de pedido de patente de invenção no prazo ordinĂĄrioDepositadaA presente invenção Ă© relacionada a fatores antimicrobianos extraĂdos de envoltĂłrios protetores (cascas) de ovos de insetos. A maioria destes fatores compreende peptĂdeos e/ou proteĂnas obtidas de tais involuntĂĄrios e/ou de seus respectivos genes codificantes, em construçÔes gĂȘnicas artificiais. SĂŁo descritas composiçÔes microbicidas e processos para sua obtenção, bem como construçÔes gĂȘnicas e processos para o controle de pestes
Does Simultaneous Computed Tomography and Quantitative Computed Tomography Show Better Prescription Rate than Dual-energy X-ray Absorptiometry for Osteoporotic Hip Fracture?
Enhancement of Gap Junction Function During Acute Myocardial Infarction Modifies Healing and Reduces Late Ventricular Arrhythmia Susceptibility
Improving compliance to osteoporosis workup and treatment in postmenopausal patients after a distal radius fracture
Connexin 32 dominant-negative mutant transgenic rats are resistant to hepatic damage by chemicals
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