Fibra de carbono en lesiones articulares de rótula de conejos

Hemos utilizado implantes de fibra de carbono para rellenar defectos osteocartilaginosos creados en la superficie articular de la rótula de 18 conejos, con el objetivo de estudiar a largo plazo la evolución histológica del proceso de reparación. A los 6 meses de evolución postoperatoria el defecto fue rellenado por tejido fibroso, a los 9 meses se desarrolló tejido fibrocartilaginoso y a los 12 meses los defectos estaban cubiertos por tejido cartilaginoso hialino.Carbon fiber implants were used to fill osteochondral defects created on the articular surface in the patella in 18 rabbits for the purpose of studying the long-term histological changes of the repair process. Six months after surgery the defect was filled by fibrous tissue, where the superficial area was organized parallel to the joint surface. Fibrocartilage developed after 9 months and, after 12 months, the defects were covered by hyaline cartilage tissue

Fabrication of ultrathin MIL-96(Al) films and study of CO2 adsorption/desorption processes using quartz crystal microbalance

This contribution reports the fabrication and characterization of ultrathin films of nanoparticles of the water stable microporous Al tricarboxylate metal organic framework MIL-96(Al). The preparation of MOF dispersions in chloroform has been optimized to obtain dense monolayer films of good quality, without nanoparticle agglomeration, at the air-water interface that can be deposited onto solid substrates of different nature without any previous substrate functionalization. The MOF studied shows great interest for CO2 capture because it presents Al3+ Lewis centers and hydroxyl groups that strongly interact with CO2 molecules. A comparative CO2 adsorption study on drop-cast, Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) films using a Quartz Crystal Microbalance-based setup (QCM) has revealed that the CO2 uptake depends strongly on the film fabrication procedure and the storage conditions. Noteworthy the CO2 adsorption capacity of LB films is increased by 30% using a simple and green treatment (immersion of the film into water during 12 h just after film preparation). Finally, the stability of LB MOF monolayers upon several CO2 adsorption/desorption cycles has been demonstrated, showing that CO2 can be easily desorbed from the films at 303 K by flowing an inert gas (He). These results show that MOF LB monolayers can be of great interest for the development of MOF-based devices that require the use of very small MOF quantities, especially gas sensors

Chicken meat and bone meal valorization by hydrothermal treatment and anaerobic digestion: Biofuel production and nutrient recovery

In this work, chicken meat and bones (C-MBM) waste is treated through a sequence of stages including hydrothermal treatment (HTT), nutrient recovery and anaerobic digestion, with the aim of evaluating their potential synergy as a circular economy approach. HTT was carried out at 170, 200 and 230 ◦C, under non-acidic and acidic conditions using 0.5 M HCl (HTT-A). Phosphorous from process water was recovered by chemical precipitation with the addition of a Mg salt, and the liquid effluent was anaerobically treated to degrade organic matter and produce a methane-rich biogas. Hydrochar obtained under non-acidic conditions presented poor combustion characteristics, while HTT-A yielded a bio-oil with high higher heating value (≈38 MJ/kg), good combustibility performance and high reactivity. More than 95% phosphorous (as phosphate) and almost 100% nitrogen (being 30% as NH4–N) content in C-MBM were solubilized in the process water upon HTT-A, while these nutrients were mainly retained in the hydrochar in non-acidic reactions. Chemical precipitation of P and NH4–N from HTT-A process water allowed recovering a crystalline solid identified as struvite and a struvite-apatite mixture, with negligible heavy metals content. High methane production (250–300 mL CH4/g CODadded) and organic matter removal (up to 75%) were achieved in the anaerobic tests. HTT proves to be a suitable treatment for material and energetic valorization of C-MBM, within a circular economy framework, which allows to obtain high value-added products (hydrochar/bio-oil, biofertilizers and biogas)Authors greatly appreciate funding from Spain’s MINECO (PID2019- 108445RB-I00, PDC2021-120755-I00), Madrid Regional Government (Project S2018/EMT-4344) and Grupo Kerbest Company. A. Sarrion wishes to thank the Spanish MICINN and ESF for a research grant (BES2017-081515). R.P. Ipiales acknowledges financial support from Community of Madrid (IND2019/AMB-17092) and Arquimea-Agrotech Compan

Coating of conducting and insulating threads with porous mof particles through langmuir-blodgett technique

The Langmuir-Blodgett (LB) method is a well-known deposition technique for the fabrication of ordered monolayer and multilayer thin films of nanomaterials onto different substrates that plays a critical role in the development of functional devices for various applications. This paper describes detailed studies about the best coating configuration for nanoparticles of a porous metal-organic framework (MOF) onto both insulating or conductive threads and nylon fiber. We design and fabricate customized polymethylmethacrylate sheets (PMMA) holders to deposit MOF layers onto the threads or fiber using the LB technique. Two different orientations, namely, horizontal and vertical, are used to deposit MIL-96(Al) monolayer films onto five different types of threads and nylon fiber. These studies show that LB film formation strongly depends on deposition orientation and the type of threads or fiber. Among all the samples tested, cotton thread and nylon fiber with vertical deposition show more homogenous monolayer coverage. In the case of conductive threads, the MOF particles tend to aggregate between the conductive thread’s fibers instead of forming a continuous monolayer coating. Our results show a significant contribution in terms of MOF monolayer deposition onto single fiber and threads that will contribute to the fabrication of single fiber or thread-based devices in the future

Inorganic phosphate modulates the expression of the NaPi-2a transporter in the trans -golgi network and the interaction with PIST in the proximal tubule

Inorganic phosphate (Pi) homeostasis is maintained by the tight regulation of renal Pi excretion versus reabsorption rates that are in turn modulated by adjusting the number of Pi transporters (mainly NaPi-2a) in the proximal tubules. In response to some hormones and a high dietary Pi content, NaPi-2a is endocytosed and degraded in the lysosomes; however, we show here that some NaPi-2amolecules are targeted to the trans-Golgi network (TGN) during the endocytosis. In the TGN, NaPi-2a interacts with PIST (PDZ-domain protein interacting specifically with TC10), a TGN-resident PDZ-domain-containing protein. The extension of the interaction is proportional to the expression of NaPi-2a in the TGN, and, consistent with that, it is increased with a high Pi diet. When overexpressed in opossum kidney (OK) cells, PIST retains NaPi-2a in the TGN and inhibits Na-dependent Pi transport. Overexpression of PIST also prevents the adaptation of OK cells to a low Pi culture medium. Our data supports the view that NaPi-2a is subjected to retrograde trafficking from the plasma membrane to the TGN using one of the machineries involved in endosomal transport and explains the reported expression of NaPi-2a in the TGN

Nonlinear analysis of a simple model of temperature evolution in a satellite

We analyse a simple model of the heat transfer to and from a small satellite orbiting round a solar system planet. Our approach considers the satellite isothermal, with external heat input from the environment and from internal energy dissipation, and output to the environment as black-body radiation. The resulting nonlinear ordinary differential equation for the satellite's temperature is analysed by qualitative, perturbation and numerical methods, which show that the temperature approaches a periodic pattern (attracting limit cycle). This approach can occur in two ways, according to the values of the parameters: (i) a slow decay towards the limit cycle over a time longer than the period, or (ii) a fast decay towards the limit cycle over a time shorter than the period. In the first case, an exactly soluble average equation is valid. We discuss the consequences of our model for the thermal stability of satellites.Comment: 13 pages, 4 figures (5 EPS files

Antagonismo in vitro de diferentes aislados de Trichoderma spp., sobre Macrophomina phaseolina.

Con este trabajo se concluye que la cepa de Trichoderma MS19 tiene un alto potencial antagónico con respecto a las demás cepas al considerar su tasa de crecimiento y porcentaje de inhibición de Macrophomina phaseolina.CONACYT - Consejo Nacional de Ciencias y TecnologíaPROCIENCI

Efectividad de AQ10 (Ampelomyces quisqualis cepa M-10) en el control biológico del oídio (Podosphaera xanthii).

El oídio de las cucurbitáceas es una enfermedad que disminuye la productividad y provoca pérdidas económicas. El control químico, incrementa costos de roducción y la resistencia de hongos; por ello se buscan alternativas como el control biológico.CONACYT - Consejo Nacional de Ciencias y TecnologíaPROCIENCI

Antagonismo in vitro de diferentes aislados de Trichoderma spp., sobre Macrophomina phaseolina.

Con este trabajo se concluye que la cepa de Trichoderma MS19 tiene un alto potencial antagónico con respecto a las demás cepas al considerar su tasa de crecimiento y porcentaje de inhibición de Macrophomina phaseolina.CONACYT - Consejo Nacional de Ciencias y TecnologíaPROCIENCI