Transgendered in Alaska: Navigating the Changing Legal Landscape for Change in Gender Petitions

Background: Detecting intracellular bacterial symbionts can be challenging when they persist at very low densities. Wolbachia, a widespread bacterial endosymbiont of invertebrates, is particularly challenging. Although it persists at high titers in many species, in others its densities are far below the detection limit of classic end-point Polymerase Chain Reaction (PCR). These low-titer infections can be reliably detected by combining PCR with DNA hybridization, but less elaborate strategies based on end-point PCR alone have proven less sensitive or less general. Results: We introduce a multicopy PCR target that allows fast and reliable detection of A-supergroup Wolbachia -even at low infection titers -with standard end-point PCR. The target is a multicopy motif (designated ARM: A-supergroup repeat motif) discovered in the genome of wMel (the Wolbachia in Drosophila melanogaster). ARM is found in at least seven other Wolbachia A-supergroup strains infecting various Drosophila, the wasp Muscidifurax and the tsetse fly Glossina. We demonstrate that end-point PCR targeting ARM can reliably detect both high-and low-titer Wolbachia infections in Drosophila, Glossina and interspecific hybrids. Conclusions: Simple end-point PCR of ARM facilitates detection of low-titer Wolbachia A-supergroup infections. Detecting these infections previously required more elaborate procedures. Our ARM target seems to be a general feature of Wolbachia A-supergroup genomes, unlike other multicopy markers such as insertion sequences (IS)

X-ray-provoked non-Mendelian transgenerational oncodeterminants

Cancer is the most important risk of radiation exposure. There is a definite lack of suitable test systems, human epidemiological data are only available for certain radiation types, especially not for charged particles. We use the Xiphophorus model which is genetically well characterized. As a prelude to experiments with heavy ions we report here on results obtained with x-rays to establish the necessary baseline for future studies. Apart from this direct aim we hope to obtain also a better insight in the genetical determination of cancer formation

Проектирование технологического процесса изготовления цилиндра и оснастки

РЕФЕРАТ Выпускная квалификационная работа 98 с., 12 рис., 27 табл., 7 источников, 6 прил. Ключевые слова: цилиндр, оснастка, литье, сверление, приспособление, технологический процесс. Объектом исследования является проектирование технологического процесса изготовления цилиндра Цель работы: проектирование технологического процесса изготовления цилиндра и оснастки В процессе исследования проводились анализ технологического процесса, технико-экономическая анализ, конструирование приспособления для сверления В результате выполнения работы был разработан технологический процесс изготовления детали цилиндр и сконструировано приспособление для сверления Основные конструктивные, технологические и технико-эксплуатационные характеристики: сконструированное приспособление для сверлABSTRACT Final qualifying work 98 p., 12 Fig., 27 tab., 7 springs, 6 ADJ. Key words: cylinder, tooling, casting, drilling, fitting, process. The object of study is (are) the Design of technological process of manufacturing of cylinder Goal – to Design the technological process of manufacturing of cylinder and snap In the process of investigation the analysis of the technological feasibility analysis, designing fixtures for drilling The result of the work was developed technological process of manufacturing parts and the cylinder fixture is designed for drilling The basic constructive, technological, technical-operational and features: the fixture is Designed for drilling consists of a casing, rod rack, cover, cylinder, prisms and the plate conductor. Level of implementation: the te

ATK-ForceField: A New Generation Molecular Dynamics Software Package

ATK-ForceField is a software package for atomistic simulations using classical interatomic potentials. It is implemented as a part of the Atomistix ToolKit (ATK), which is a Python programming environment that makes it easy to create and analyze both standard and highly customized simulations. This paper will focus on the atomic interaction potentials, molecular dynamics, and geometry optimization features of the software, however, many more advanced modeling features are available. The implementation details of these algorithms and their computational performance will be shown. We present three illustrative examples of the types of calculations that are possible with ATK-ForceField: modeling thermal transport properties in a silicon germanium crystal, vapor deposition of selenium molecules on a selenium surface, and a simulation of creep in a copper polycrystal.Comment: 28 pages, 9 figure

Deposition of Airborne Particles onto the Human Eye - Wind Tunnel Studies of the Deposition Velocity onto the Eyes of a Mannequin

Abstract In the field of occupational hygiene much consideration is devoted to the uptake through the human airways, but there are also reports of irritations, complaints and hazards or potential hazards due to particle deposition onto the eyes. There is a need for data regarding the dependence of the deposition rate on particle size and environmental parameters in order to formulate sampling criteria and to find dose-response relations. The deposition of airborne particles onto the human eye was studied by making use of a dummy, the eyes of which were covered with pieces of transparent sticky foil. The dummy was exposed to airborne particles in a wind tunnel. The airborne particle concentration was simultaneously measured using an Aerodynamic Particle Sizer (APS, TSI Inc., U.S.A.). The deposited particles were counted using an optical microscope equipped with an image analyser. For the particle sizes (2–30 μm), wind velocities (0.5 and 1.0 m s−1), wind directions (0, 90 and 180°) and turbulence intensities (1.3 and 19%) employed in this study, deposition velocities onto the eyes were determined to be in the range of 0.001–1 cm s−1

Evaluation of railway capacity

This paper describes the relatively new UIC 406 method for calculating capacity consumption on railway lines. The UIC 406 method is an easy and effective way of calculating the capacity consumption, but it is possible to expound the UIC 406 method in different ways which can lead to different capacity consumptions. This paper describes the UIC 406 method and how it is expounded in Denmark. The paper describes the importance of choosing the right length of the line sections examined and how line sections with multiple track sections are examined. Furthermore, the possibility of using idle capacity to run more trains is examined. The paper presents a method to examine the expected capacity utilization of future timetables. The method is based on the plan of operation instead of the exact (known) timetable. At the end of the paper it is described how it is possible to make capacity statements of a railway network. Some of the aspects which have to be paid attention to making annual capacity statements are presented too

Singleshot polychromatic coherent diffractive imaging with a high-order harmonic source

© 2020 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.Singleshot polychromatic coherent diffractive imaging is performed with a high-intensity high-order harmonic generation source. The coherence properties are analyzed and several reconstructions show the shot-to-shot fluctuations of the incident beam wavefront. The method is based on a multi-step approach. First, the spectrum is extracted from double-slit diffraction data. The spectrum is used as input to extract the monochromatic sample diffraction pattern, then phase retrieval is performed on the quasi-monochromatic data to obtain the sample’s exit surface wave. Reconstructions based on guided error reduction (ER) and alternating direction method of multipliers (ADMM) are compared. ADMM allows additional penalty terms to be included in the cost functional to promote sparsity within the reconstruction

Modeling and Control of a Piezo-Actuated High-Dynamic Compensation Mechanism for Industrial Robots

This paper presents a method for modeling and control of a piezo-actuated high-dynamic compensation mechanism (HDCM) for usage together with an industrial robot during a machining operation, such as milling in aluminum. The spindle is attached to the compensation mechanism and the robot holds the workpiece. Due to the inherent resonant character of mechanical constructions of this type, and the nonlinear phenomena appearing in piezo actuators, control of the compensation mechanism is a challenging problem. This paper presents models of the construction, experimentally identified using subspace-based identification methods. A subsequent control scheme, based on the identified models, utilizing state feedback for controlling the position of the spindle is outlined. Experimental results performed on a prototype of the HDCM are also provided

Western Region Health & Wellness Programming Efforts: Qualitative Results from a Three-Part Listening Session

The purpose of this project was to understand the programming efforts and experiences of Extension professionals in the Western Region of the United States. The Health and Wellness Working Group team held a series of listening sessions with a closed cohort of Extension professionals, hoping to learn from each other while also building the foundation for future collaborations and conversations about health and wellness programing in the western region. The objectives were to understand: (1) the state of health and wellness work in the western region, including the gaps and challenges, (2) the programs that currently exist and ways that collaborations happen across Extension, (3) strategies for moving health and wellness programming forward across the western region