Variantes genéticas en miRNAs implicadas en la susceptibilidad a desarrollar Leucemia Linfática Crónica

El objetivo principal del estudio es identificar nuevos marcadores de riesgo en LLC, analizando variantes genéticas en pre-miRNAs que regulan genes implicados en el desarrollo de la LLC. Además vamos a intentar entender su papel en el desarrollo de la enfermeda

Tumor xenograft modeling identifies an association between TCF4 loss and breast cancer chemoresistance

Understanding the mechanisms of cancer therapeutic resistance is fundamental to improving cancer care. There is clear benefit from chemotherapy in different breast cancer settings; however, knowledge of the mutations and genes that mediate resistance is incomplete. In this study, by modeling chemoresistance in patientderived xenografts (PDXs), we show that adaptation to therapy is genetically complex and identify that loss of transcription factor 4 (TCF4; also known as ITF2) is associated with this process. A triple-negative BRCA1-mutaied PDX was used to study the genetics of chemoresistance. The PDX was treated in parallel with four chemotherapies for five iterative cycles. Exome sequencing identified few genes with de novo or enriched mutations in common among the different therapies, whereas many common depleted mutations/ genes were observed. Analysis of somatic mutations from The Cancer Genome Atlas (TCGA) supported the prognostic relevance of the identified genes. A mutation in TCF4 was found de novo in all treatments, and analysis of drug sensitivity profiles across cancer cell lines supported the link to chemoresistance. Loss of TCF4 conferred chemoresistance in breast cancer cell models, possibly by altering cell cycle regulation. Targeted sequencing in chemoresistant tumors identified an intronic variant of TCF4 that may represent an expression quantitative trait locus associated with relapse outcome in TCGA. Immunohistochemical studies suggest a common loss of nuclear TCF4 expression post-chemotherapy. Together, these results from tumor xenograft modeling depict a link between altered TCF4 expression and breast cancer chemoresistance

Endothelial Progenitor Cells Predict Cardiovascular Events after Atherothrombotic Stroke and Acute Myocardial Infarction. A PROCELL Substudy.

Introduction: The aim of this study was to determine prognostic factors for the risk of new vascular events during the first 6 months after acute myocardial infarction (AMI) or atherothrombotic stroke (AS). We were interested in the prognostic role of endothelial progenitor cells (EPC) and circulating endothelial cells (CEC). Methods: Between February 2009 and July 2012, 100 AMI and 50 AS patients were consecutively studied in three Spanish centres. Patients with previously documented coronary artery disease or ischemic strokes were excluded. Samples were collected within 24h of onset of symptoms. EPC and CEC were studied using flow cytometry and categorized by quartiles. Patients were followed for up to 6 months. NVE was defined as new acute coronary syndrome, transient ischemic attack (TIA), stroke, or any hospitalization or death from cardiovascular causes. The variables included in the analysis included: vascular risk factors, carotid intima-media thickness (IMT), atherosclerotic burden and basal EPC and CEC count. Multivariate survival analysis was performed using Cox regression analysis. Results: During follow-up, 19 patients (12.66%) had a new vascular event (5 strokes; 3 TIAs; 4 AMI; 6 hospitalizations; 1 death). Vascular events were associated with age (P = 0.039), carotid IMT≥0.9 (P = 0.044), and EPC count (P = 0.041) in the univariate analysis. Multivariate Cox regression analysis showed an independent association with EPC in the lowest quartile (HR: 10.33, 95%CI (1.22-87.34), P = 0.032] and IMT≥0.9 [HR: 4.12, 95%CI (1.21-13.95), P = 0.023]. Conclusions: Basal EPC and IMT≥0.9 can predict future vascular events in patients with AMI and AS, but CEC count does not affect cardiovascular risk

Variantes genéticas en miRNAs implicadas en la susceptibilidad a desarrollar Leucemia Linfática Crónica

El objetivo principal del estudio es identificar nuevos marcadores de riesgo en LLC, analizando variantes genéticas en pre-miRNAs que regulan genes implicados en el desarrollo de la LLC. Además vamos a intentar entender su papel en el desarrollo de la enfermeda

Attalea: Revista de Divulgación Científica n° 1 (2023)

Hongos de micorriza arbuscular en agroecosistemas de cacao: abundancia y diversidad. Ranitomeya flavovittata: una belleza de patas doradas endémica de Loreto. Sapos, ranas, lluvias y sequías: historias de cambio climático en la Amazonía peruana a través de los anfibios. Huacapú (Minquartia guianensis aubl): especie amazónica resiliente a zonas inundables temporalmente. Resiliencia de especies forestales en áreas inundables de la Amazonía peruana. ¿Selva productiva? La entrevista Dr. Rodrigo Arce Rojas. El lenguaje oculto de los árboles. Biblioteca de la Ciencia: conocimientos indígenas