Assessment of solar driven TiO2-assisted photocatalysis efficiency on amoxicillin degradation

The objective of this work was to evaluate the efficiency of a solar TiO2-assisted photocatalytic process on amoxicillin (AMX) degradation, an antibiotic widely used in human and veterinary medicine. Firstly, solar photolysis of AMX was compared with solar photocatalysis in a compound parabolic collectors pilot scale photoreactor to assess the amount of accumulated UV energy in the system (Q (UV)) necessary to remove 20 mg L-1 AMX from aqueous solution and mineralize the intermediary by-products. Another experiment was also carried out to accurately follow the antibacterial activity against Escherichia coli DSM 1103 and Staphylococcus aureus DSM 1104 and mineralization of AMX by tracing the contents of dissolved organic carbon (DOC), low molecular weight carboxylate anions, and inorganic anions. Finally, the influence of individual inorganic ions on AMX photocatalytic degradation efficiency and the involvement of some reactive oxygen species were also assessed. Photolysis was shown to be completely ineffective, while only 3.1 kJ(UV) L-1 was sufficient to fully degrade 20 mg L-1 AMX and remove 61 % of initial DOC content in the presence of the photocatalyst and sunlight. In the experiment with an initial AMX concentration of 40 mg L-1, antibacterial activity of the solution was considerably reduced after elimination of AMX to levels below the respective detection limit. After 11.7 kJ(UV) L-1, DOC decreased by 71 %; 30 % of the AMX nitrogen was converted into ammonium and all sulfur compounds were converted into sulfate. A large percentage of the remaining DOC was in the form of low molecular weight carboxylic acids. Presence of phosphate ions promoted the removal of AMX from solution, while no sizeable effects on the kinetics were found for other inorganic ions. Although the AMX degradation was mainly attributed to hydroxyl radicals, singlet oxygen also plays an important role in AMX self-photosensitization under UV/visible solar light

Process enhancement at near neutral pH of a homogeneous photo-Fenton reaction using ferricarboxylate complexes: Application to oxytetracycline degradation

This work demonstrates the application at near neutral pH of a photo-Fenton reaction mediated by ferricarboxylates on the treatment of aqueous solutions containing the antibiotic Oxytetracycline (OTC) under solar irradiation. The formation of a Fe:OTC complex after Fe2+ oxidation to Fe3+, in the presence of H2O2, showed the inconvenience of using the conventional Fe2+/H2O2/UV-Vis process at near neutral pH levels, as the complex is retained in the filter. To overcome this, a Fe3+/Oxalate/UV-Vis or Fe3+/Citrate/H2O2/UV-Vis process was proposed. The higher tendency of Fe3+ to form complexes with carboxylates avoids the formation of Fe:OTC complexes and allows for proper OTC detection along reaction times. The photo-Fenton process itself is improved by the extension of the iron solubility to higher and more practical pH values, by the increase of the quantum yield of Fe2+ production and by presenting stronger radiation absorption at wavelengths up to 580 nm. In this way, process efficiency was evaluated for different variables such as Fe3+ concentration, pH, temperature and irradiance, using a compound parabolic collector (CPC) photoreactor at lab-scale under simulated solar radiation. Reaction rates were compared in the presence of different inorganic anions and humic acids, and in two different real wastewater matrixes. Results obtained in a CPC pilot-scale plant under natural solar light, using an iron/oxalate molar rati

Superior operational stability of immobilized L-asparaginase over surface-modified carbon nanotubes

L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the L-asparagine hydrolysis into L-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some disadvantages are associated with its free form, such as the ASNase short half-life, which may be overcome by enzyme immobilization. In this work, the immobilization of ASNase by adsorption over pristine and modified multi-walled carbon nanotubes (MWCNTs) was investigated, the latter corresponding to functionalized MWCNTs through a hydrothermal oxidation treatment. Different operating conditions, including pH, contact time and ASNase/MWCNT mass ratio, as well as the operational stability of the immobilized ASNase, were evaluated. For comparison purposes, data regarding the ASNase immobilization with pristine MWCNT was detailed. The characterization of the ASNase-MWCNT bioconjugate was addressed using different techniques, namely Transmission Electron Microscopy (TEM), Thermogravimetric Analysis (TGA) and Raman spectroscopy. Functionalized MWCNTs showed promising results, with an immobilization yield and a relative recovered activity of commercial ASNase above 95% under the optimized adsorption conditions (pH 8, 60 min of contact and 1.5 × 10-3 g mL-1 of ASNase). The ASNase-MWCNT bioconjugate also showed improved enzyme operational stability (6 consecutive reaction cycles without activity loss), paving the way for its use in industrial processes.publishe

Immobilization of L-asparaginase towards surface-modified carbon nanotubes

L-asparaginase (LA) is an enzyme that catalyzes L-asparagine hydrolysis into L-aspartic acid and ammonia and is mainly applied in pharmaceutical and food industries. The LA currently commercialized for pharmaceutical purposes is produced from two main bacterial sources: recombinant Escherichia coli and Erwinia chrysanthemi. However, some disadvantages are associated with its free form, such as the shorter half-life. Immobilization of LA has been proposed as an efficient approach to overcome this limitation. In this work, a straightforward method, including the functionalization of multi-walled carbon nanotubes (MWCNTs) through a hydrothermal oxidation treatment and the immobilization of LA by adsorption over pristine and modified MWCNTs was investigated. Different operation conditions, including pH, contact time, ASNase/MWCNT mass ratio, and the operational stability of the immobilized LA, were evaluated. The characterization of the LA-MWCNT bioconjugate was addressed using different techniques, namely Transmission Electron Microscopy (TEM), Thermogravimetric analysis (TGA), and Raman spectroscopy. Functionalized MWCNTs showed promising results, with an immobilization yield and a relative recovered activity of commercial LA above 95%, under the optimized adsorption conditions (pH 8, 60 min of contact, and 1.510–3 g.mL-1 of LA). The LA-MWCNT bioconjugate also showed improved enzyme operational stability (6 consecutive reaction cycles without activity loss), proving its suitability for application in industrial processes.publishe

Immobilization of L-asparaginase towards surface-modified carbon nanotubes

L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes L-asparagine hydrolysis into L-aspartic acid and ammonia and is mainly applied in pharmaceutical and food industries [1]. The ASNase currently commercialized for pharmaceutical purposes is produced from two main bacterial sources: recombinant Escherichia coli and Erwinia chrysanthemi. However, some disadvantages are associated with its free form, such as the shorter half-life [2]. Immobilization of ASNase has been proposed as an efficient approach to overcome this limitation [3]. In this work, a straightforward method, including the functionalization of multi-walled carbon nanotubes (MWCNTs) through a hydrothermal oxidation treatment with nitric acid, and the immobilization of ASNase by adsorption over pristine and modified MWCNTs was investigated. Different operation conditions, including pH, contact time, ASNase/MWCNT mass ratio, and the operational stability of the immobilized ASNase were evaluated. The characterization of the ASNase-MWCNT bioconjugate was addressed using different techniques, namely Transmission Electron Microscopy (TEM), Thermogravimetric analysis (TGA), and Raman spectroscopy. Functionalized MWCNTs showed promising results, with an immobilization yield and a relative recovered activity of commercial ASNase above 95%, under the optimized adsorption conditions (pH 8, 60 min of contact and 1.5´10–3 g.mL-1of ASNase). The ASNase-MWCNT bioconjugate also showed improved enzyme operational stability (6 consecutive reaction cycles without activity loss), proving its suitability for application in industrial processes.publishe

Wireless power transmission: R&D activities within Europe

Wireless power transmission (WPT) is an emerging technology that is gaining increased visibility in recent years. Efficient WPT circuits, systems and strategies can address a large group of applications spanning from batteryless systems, battery-free sensors, passive RF identification, near-field communications, and many others. WPT is a fundamental enabling technology of the Internet of Things concept, as well as machine-to-machine communications, since it minimizes the use of batteries and eliminates wired power connections. WPT technology brings together RF and dc circuit and system designers with different backgrounds on circuit design, novel materials and applications, and regulatory issues, forming a cross disciplinary team in order to achieve an efficient transmission of power over the air interface. This paper aims to present WPT technology in an integrated way, addressing state-of-the-art and challenges, and to discuss future R&D perspectives summarizing recent activities in Europe.The work of N. Borges Carvalho and A. J. S. Soares Boaventura was supported by the Portuguese Foundation for Science and Technology (FCT) under Project CREATION EXCL/EEI-TEL/0067/2012 and Doctoral Scholarship SFRH/BD/80615/2011. The work of H. Rogier was supported by BELSPO through the IAP Phase VII BESTCOM project and the Fund for Scientific Research-Flanders (FWO-V). The work of A. Georgiadis and A. Collado was supported by the European Union (EU) under Marie Curie FP7-PEOPLE-2009-IAPP 251557 and the Spanish Ministry of Economy and Competitiveness Project TEC 2012-39143. The work of J. A. García and M. N. Ruíz was supported by the Spanish Ministries MICINN and MINECO under FEDER co-funded Project TEC2011-29126-C03-01 and Project CSD2008-00068. The work of J. Kracek and M. Mazanek was supported in part by the Czech Ministry of Education Youth and Sports under Project OC09075–Novel Emerging Wireless Systems

Cidadania mediada : processos de democratização da política municipal no Brasil

This article discusses the notion that the persistence of &ldquo;traditional&rdquo; political practices weakens Brazil&rsquo;s democracy.Drawing on the cases of three Brazilian municipalities administered by the Workers&rsquo; Party (PT), the author examines the space between &ldquo;traditional&rdquo; and &ldquo;modern&rdquo; and argues that successful democratization does not eradicate practices such as clientelism and patronage, but it tends to incorporate and build on these traditional political elements. Moreover, the article maintains that the democratization of municipal politics is inextricably bound up with the eradication of poverty and the construction of a responsive, state-based social safety net.<br /

O Design Universal Para Apprendizagem e a Pedagogia das Estacoes: As Multiplas Temporalidades / Espacialidades do Aprender nas Escolas.

This article explores school inclusion processes, with a view to problematizing the learning hegemonies of time and space in educational institutions. It presents partial results of the research project; 'A Escola Para Todos' (The School for All) and responds to the question: How can Universal Design for Learning (UDL), which is somewhat similar to the 'pedagogy of the seasons,' qualify inclusive experiences in schools? By providing an integrative review of the salient features of UDL and pedagogy of the seasons, the paper questions the hegemony of time-space teaching and learning in schools by drawing on narrative theory. Methodologically, this part of the research provides an integrated reflection on critical conceptual attributes incorporating; UDL, the temporalities and spatialities of learning, and concepts of inclusive education. The results of this stage of the research show that it is necessary to overcome the hegemony of time and linear space in learning and teaching processes in order to arrive at school inclusion. Despite similarities between UDL and 'pedagogy of the seasons' tensions remain between these approaches. Not least as reflected in the overcoming of paradoxes such as the necessity to address universal requirements while also tending to particular learner requirements, or equally, while recognizing the variability of strategies without losing sight of more global pedagogical approaches. This challenge is accentuated when the UDL concept of 'expert learners' is further interrogated