50 research outputs found
The application of NMR and FTIR spectroscopic techniques results in the multivariate data analysis for propolis classification
У овом раду су приказане брзе и једноставне спектроскопске методе за
одређивање хемијског састава прополиса типа топола са подручја Србије, Босне и
Херцеговине и Бугарске коришћењем метаболомичког приступа. У циљу
корелисања разлика у хемијском саставу прополиса топола типа са надморском
висином са које су узорци прикупљени, коришћени су резултати NMR, IR и UV
спектроскопских техника у комбинацији са PCA и OPLS статистичким методама.
Ботаничко порекло прополиса је утврђено поређењем спектралних података
узорака прополиса са спектралним подацима фенолних смола пупољака
различитих врста топола које расту у близини локација са којих су узорци
прикупљени. За корелисање две матрице (табеле) спектралних података
коришћена је O2PLS метода. На основу примењених статистичких анализа (PCA,
OPLS i O2PLS), главне компоненте у узорцима прополиса прикупљеним у регији
умерено-континенталне климе на надморској висини изнад 500 m су ацетил
глицерил естри фенолних киселина са пупољака Populus tremula. Флавоноиди
фенолних смола пупољака врста P. nigra и P. x euramericana су најзаступљенији у
узорцима прополиса прикупљеним на надморској висини испод 400 m. Узорци
прикупљени на надморској висини између 400 и 500 m садрже све поменуте
секундарне метаболите топола у различитим односима.
Применом OPLS-DA методе на спектралне податке узорци прополиса типа
топола су класификовани у две класе назване Планински прополис типа топола и
Равничарски прополис типа топола.
Антимикробна активност прикупљених узорака је тестирана на Грам-
позитивним бактеријским сојевима Staphylococcus aureus (ATCC 6538),
клиничком изолату Bacillus cereus, Micrococcus flavus (ATCC 10240) и Listeria
monocytogenes (NCTC 7973), Грам-негативним бактеријама Pseudomonas
aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC 13311), Escherichia coli
(ATCC 35210) и хуманом изолату Enterobacter cloacae и на гљиви Fusarium
oxysporum. Резултати антимикробних тестова показали су високу антимикробну
активност прополиса према свим испитиваним микроорганизмима.Here we have presented simple, fast and rapid spectroscopic methods for
determination of the chemical composition of Poplar type propolis from Serbia, Bosnia
and Herzegovina and Bulgaria, using metabolomics approach. The main objective was
to correlate variability in Poplar type propolis composition with the altitude of its
collection. This was carried out using NMR, IR, and UV spectroscopy followed by PCA
and OPLS methods. The botanical origin of the propolis was established by comparing
spectral data of propolis with those of various Populus species growing close to the
collection site. An O2PLS method was utilized to integrate two blocks of data.
According to PCA, OPLS and O2PLS, the major compounds in propolis samples,
collected from temperate continental climate above 500 m, were phenolic glycerides
originating from Populus tremula buds. Flavonoids were predominant in propolis
samples collected below 400 m, originating from P. nigra and P. x euramericana buds.
Samples collected at 400 – 500 m were of mixed origin with variable amount of all
detected metabolites.
Using OPLS-DA method and spectral data, poplar propolis samples were
classified in two classes named Mountain populus type and Flatland populus type.
Antimicrobial activity of propolis samples was tested on Gram-positive bacteria
Staphylococcus aureus (ATCC 6538), clinical isolates of Bacillus cereus, Micrococcus
flavus (ATCC 10240) and Listeria monocytogenes (NCTC 7973), Gram-negative
bacteria Pseudomonas aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC
13311), Escherichia coli (ATCC 35210), and human isolate of Enterobacter cloacae,
on fungus Fusarium oxysporum. The results of antimicrobial tests showed strong
antimicrobial activity of propolis against all tested microorganisms
Supplementary material for the article: Rašković, B.; Vatić, S.; Andelković, B.; Blagojević, V.; Polović, N. Optimizing Storage Conditions to Prevent Cold Denaturation of Trypsin for Sequencing and to Prolong Its Shelf Life. Biochemical Engineering Journal 2016, 105, 168–176. https://doi.org/10.1016/j.bej.2015.09.018
Supplementary material for: [https://doi.org/10.1016/j.bej.2015.09.018]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2024
AUTOMATIC DETERMINATION OF FILTER COEFFICIENTS FOR LOCAL CONTRAST ENHANCEMENT
This study proposes an algorithm whose main advantage is in enabling the automatic determination of non-linear homomorphic filter coefficients used for local contrast enhancement in digital image processing. The presented algorithm is tested in a real production environment. The obtained results are compared with relevant examples in literature, showing the advantages of the achieved results or a relatively high level of their correspondence with reference results. The proposed procedure can be used for various applications in mechatronics, robotics and automatized production systems
HPLC/MS-TOF Analysis of Surface Resins from Three Poplar Clones Grown in Serbia
Background and Purpose: Poplar clones grown in Serbia are fast growing tree species important for many different purposes in forestry and industry. In this study chemical content of the surface resins of three poplar clones grown in Serbia -M1, B229 and PE 19/66 was analyzed, aiming at their potential usage as a source of natural products important for pharmacy and chemotaxonomy. Materials and Methods: Using HPLC/MS-TOF we gained the first information on chemical compounds which comprise of resins on terminal twigs cuttings of commonly grown poplar clones. Provided from the nursery of the Institute of Lowland Forestry and Environment (Serbia), terminal twigs cuttings with leaves of different development stage from two year old seedlings of M1 poplar clone (Populus euramericana L.), PE 19/66 clone and B229 clone (both belonging to Populus deltoides) were sampled. The washing of the surface resins from terminal twigs cuttings of every sample was done with methylene-chloride until the samples were prepared for HPLC/MS-TOF analysis. Results: Out of 38 different compounds which were identified, M1 clone qualitatively differed for 14 compounds as compared to two other clones. Generally, the results showed that the composition of the resins consisted of different phenolic acids, phenolic esters, flavonoids and other contents. Conclusion: These three poplar clones are potent producers of pharmacologically and chemotaxonomically potent compounds in forest ecosystems, especially M1 clone
Supplementary data for article: Andelkovic, B.; Vujisić, L. V.; Vučković, I. M.; Tešević, V.; Vajs, V.; Godevac, D. Metabolomics Study of Populus Type Propolis. Journal of Pharmaceutical and Biomedical Analysis 2017, 135, 217–226. https://doi.org/10.1016/j.jpba.2016.12.003
Supplementary material for: [https://doi.org/10.1016/j.jpba.2016.12.003]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2386]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3017
Supplementary data for article: Novakovic, M.; Bukvicki, D.; Andjelkovic, B.; Ilic-Tomic, T.; Veljic, M.; Tesevic, V.; Asakawa, Y. Cytotoxic Activity of Riccardin and Perrottetin Derivatives from the Liverwort Lunularia Cruciata. Journal of Natural Products 2019, 82 (4), 694–701. https://doi.org/10.1021/acs.jnatprod.8b00390
Supporting information for: [https://doi.org/10.1021/acs.jnatprod.8b00390]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3096
Supplementary data for the article: Krstić, G.; Anđelković, B.; Choi, Y. H.; Vajs, V.; Stević, T.; Tešević, V.; Gođevac, D. Metabolic Changes in Euphorbia Palusrtis Latex after Fungal Infection. Phytochemistry 2016, 131, 17–25. https://doi.org/10.1016/j.phytochem.2016.08.005
Supplementary material for: [https://doi.org/10.1016/j.phytochem.2016.08.005]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2335
Supplementary material for the article: Cvetković, M.; Anđelković, B.; Stevanović, V.; Jadranin, M.; Đorđević, I.; Tešević, V.; Milosavljević, S.; Gođevac, D. NMR-Based Metabolomics Study of Amphoricarpos Species from Montenegro. Phytochemistry Letters 2018, 25, 1–5. https://doi.org/10.1016/j.phytol.2018.03.013
Supplementary material for: [https://doi.org/10.1016/j.phytol.2018.03.013]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2148
Supplementary material for the article: Cvetković, M.; Anđelković, B.; Stevanović, V.; Jadranin, M.; Đorđević, I.; Tešević, V.; Milosavljević, S.; Gođevac, D. NMR-Based Metabolomics Study of Amphoricarpos Species from Montenegro. Phytochemistry Letters 2018, 25, 1–5. https://doi.org/10.1016/j.phytol.2018.03.013
Supplementary material for: [https://doi.org/10.1016/j.phytol.2018.03.013]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2148
Примена lc–ms/ms технике са јонском покретљивошћу за хемијску анализу екстраката прополиса са антимикробним потенцијалом.
The objective of this study was to test four-dimensional LC-ESI-MS/MS chromatography in analysis of complex mixture such as ethanol extracts of different propolis samples. In total more than 1200 picks were identified and only for 185 literature conformation was found. The given data represent the result of tentative identification, and summarized results are given in the text. Comparing the samples, from different altitudes, 96 components were detected as characteristic in high altitude samples and 18 in samples collected at low altitudes. Antimicrobial activity of ethanol extracts of propolis (EEP) and propylene glycol extracts of propolis (PGEP) were carried out on S. aureus, B. cereus, M. flavus, L. monocytogenes, P. aeruginosa, S. typhimurium, E. coli and E. cloacae bacterial strains and compared with broad-spectrum antibiotics, streptomycin and ampicillin. Anti-quorum sensing activity was performed on P. aeruginosa by testing the effect of representative propolis extracts on biofilm formation, twitching and motility activity and production of pyocyanin. We demonstrated that the majority of explored propolis extracts have greater or equal minimal inhibitory concentration and minimum bactericidal concentration values compared to antibiotics, independently of the solvent used for the extraction. The samples collected from the highest altitude emerged as least active antimicrobial agents but with the greatest potential as anti-quorum sensing agents.Циљ овог истраживања је билo тестирање четвородимензионалне LC–ESI-MS/MS хроматографије у анализи комплексних смеша, као што су етанолни екстракти различитих узорака прополиса. Укупно је идентификовано више од 1200 пикова, а самo за 185 смо нашли литературну потврду. Приказани подаци представљају тентативну идентификацију и сумирани резултат је дат у тексту. Поређењем узорака са различитих надморских висина, за 96 једињења је утврђено да се налазе само у узорцима са високе надморске висине и 18 само у узорцима прикупљеним на нижим надморским висинама. Антибактеријске активности етанолних и пропиленгликолних екстрактата прополиса (EEP и PGEP, редом) тестиране су на S. aureus, B. cereus, M. flavus, L. monocytogenes, P. aeruginosa, S. typhimurium, E. coli и E. cloacae бактеријским линијама и поређене са анти- биотицима широког спектра деловања, стрептомицином и ампицилином. Anti-quorum sensing активност је тестирана на P. aeruginosa испитивањем ефекта репрезентативних екстраката прополиса на формирање биофилма, тестовима покретљивости руба коло- није (twitching и mobility) и производње пиоцианина. Показали смо да највећи број, коришћених екстраката прополиса, има истe и/или мање MIC и MBC вредности, у поре- ђењу са атибиотицима, независно од растварача коришћеног за екстракцију. Узорци прикупљени на високим надморским висинама су се показали као најмање активни антибактерициди али имају велики anti-quorum sensing потенцијал.Part of the theme issue honoring Professor Emeritus Slobodan Milosavljevićs 80th birthday