9 research outputs found
Molekul oÅ”teÄenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) u urinu kod bolesnika sa karcinomom svetlih Äelija bubrega - potencijalni neiznvazivni biomarkeri
Background/Aim. Kidney injury molecule-1 (KIM-1) and aquaporin-1 (AQP-1) are potential early urinary biomarkers of clear renal cell carcinoma (cRCC). The aim of this study was to ascertain relationship between the urine concentrations KIM-1 and AQP-1 with tumor size, grade, pT stage and type of operation (radical or partial nephrectomy) in patients with cRCC. Methods. Urinary concentrations of urinary KIM-1 (uKIM-1) and urinary AQP-1 (uAQP-1) were determined by commercially available ELISA kits. The analysis included 40 patients undergoing partial or radical nephrectomy for cRCC and 40 age- and sex-matched healthy adult volunteers. Results. The median preoperative concentrations of KIM-1 in the cRCC group [0.724 Ā± 1.120 ng/mg urinary creatinine (Ucr)] were significantly greater compared with controls (healthy volunteers) (0.210 Ā± 0.082 ng/mgUcr) (p = 0.0227). Postoperatively, uKIM-1 concentration decreased significantly to control values (0.177 Ā± 0.099 ng/mgUcr vs 0.210 Ā± 0.082 ng/mgUcr, respectively). The size, grade and stage of tumor were correlated positively with preoperative uKIM-1 concentrations. Contrary to these results, concentrations of uAQP-1 in the cRCC group were significantly lower (0.111 Ā± 0.092 ng/mgUcr) compared with the control group (0.202 Ā± 0.078 ng/mgUcr) (p = 0.0014). Postoperatively, the concentrations of uAQP-1 increased progressively up to control values, approximately. We find no significant correlation between preoperative uAQP-1 concentrations and tumor size, grade and stage. Conclusion. uKIM-1 was found to be a reliable diagnostic marker of cRCC, based on its significantly increased values before and decreased values after the nephrectomy.Uvod/Cilj. Molekul oÅ”teÄenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) su potencijalni rani biomarkeri karcinoma svetlih Äelija (cRCC). Cilj ove studije bio je da se utvrdi povezanost izmeÄu koncentracija KIM-1 i AQP-1 u urinu i veliÄine, gradusa, stadijuma i vrste operacije (radikalna ili parcijalna nefrektomija) kod bolesnika sa cRCC. Metode. Urinarne koncentracije KIM-1 i AQP-1 odreÄene su primenom komercijalnih ELISA kitova. Analizom je bilo obuhvaÄeno 40 bolesnika koji su bili podvrgnuti parcijalnoj ili radikalnoj nefrektomiji zbog tumora bubrega i 40 zdravih odraslih ispitanika. Grupe su bile komparabilne po polu i godinama života. Rezultati. Srednja preoperativna koncentracija urinarnog KIM-1 (uKIM-1) u cRCC grupi [0,724 Ā± 1,120 ng/mg kreatinia u urinu (Ucr)] bila je statistiÄki znaÄajno viÅ”a u poreÄenju sa koncentracijom u kontrolnoj grupi (0,210 Ā± 0,082 ng/mgUcr ) (p = 0,0227). Postoperativno, koncentracija uKIM-1 znaÄajno je padala i približavala se vrednosti u kontrolnoj grupi (0,177 Ā± 0,099 ng/mgUcr nasuprot 0,210 Ā± 0,082 ng/mgUcr). VeliÄina, gradus i stadijum tumora bili su u pozitivnoj korelaciji sa preoperativnim koncentracijama uKIM-1. Nasuprot ovim rezultatima, koncentracija urinarnog AQP-1 (uAQP-1) u cRCC grupi bila je znaÄajno niža (0,111 Ā± 0,092 ng/mgUcr) u poreÄenju sa kontrolnom grupom zdravih osoba (0,202 Ā± 0,078 ng/mgUcr) (p = 0,0014). Postoperativno, koncentracija uAQP-1 progresivno se poveÄavala, približno do vrednosti u kontrolnoj grupi. Nismo naÅ”li znaÄajnu korelaciju izmeÄu preoperativnih koncentracija uAQP-1 i veliÄine, gradusa i stadijuma tumora. ZakljuÄak. uKIM-1 bi mogao biti dodatni pouzdani dijagnostiÄki marker za cRRC na osnovu njegove znaÄajno viÅ”e preoperativne koncentracije i sniženja vrednosti nakon nefrektomije
Tissue and urinary kim-1 relate with tumor characteristics in patients with clear renal cell carcinoma
Simulation studies of the laser ablation ion source at the SHIPTRAP setup
A gas-filled miniature Radio-Frequency Quadrupole (mini-RFQ) was recently implemented into the SHIPTRAP laser ablation ion source to thermalize the laser-ablated ions and thus improve production efficiency as well as sample preparation. This source provides reference ions of various elements for online experiments with the SHIPTRAP mass spectrometer. In addition, it can be used to provide long-lived rare and radioactive isotopes available only in small sample sizes for high-precision mass measurements or to study systematic uncertainties. The performance of the laser ablation ion source was simulated using the COMSOL Multiphysics modeling software package. These studies indicate that a revised mechanical geometry and an optimized RF field improve the performance significantly
Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers
Background/Aim. Kidney injury molecule-1 (KIM-1) and aquaporin-1 (AQP-1) are
potential early urinary biomarkers of clear renal cell carcinoma (cRCC). The
aim of this study was to ascertain relationship between the urine
concentrations KIM-1 and AQP-1 with tumor size, grade, pT stage and type of
operation (radical or partial nephrectomy) in patients with cRCC. Methods.
Urinary concentrations of urinary KIM-1 (uKIM-1) and urinary AQP-1 (uAQP-1)
were determined by commercially available ELISA kits. The analysis included
40 patients undergoing partial or radical nephrectomy for cRCC and 40 age-
and sex-matched healthy adult volunteers. Results. The median preoperative
concentrations of KIM-1 in the cRCC group [0.724 Ā± 1.120 ng/mg urinary
creatinine (Ucr)] were significantly greater compared with controls (healthy
volunteers) (0.210 Ā± 0.082 ng/mgUcr) (p = 0.0227). Postoperatively, uKIM-1
concentration decreased significantly to control values (0.177 Ā± 0.099
ng/mgUcr vs 0.210 Ā± 0.082 ng/mgUcr, respectively). The size, grade and stage
of tumor were correlated positively with preoperative uKIM-1 concentrations.
Contrary to these results, concentrations of uAQP-1 in the cRCC group were
significantly lower (0.111 Ā± 0.092 ng/mgUcr) compared with the control group
(0.202 Ā± 0.078 ng/mgUcr) (p = 0.0014). Postoperatively, the concentrations
of uAQP-1 increased progressively up to control values, approximately. We
find no significant correlation between preoperative uAQP-1 concentrations
and tumor size, grade and stage. Conclusion. uKIM-1 was found to be a
reliable diagnostic marker of cRCC, based on its significantly increased
values before and decreased values after the nephrectomy. [Projekat
Ministarstva nauke Republike Srbije, br. III41018
Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma
The objective of this prospective follow-up trial was to ascertain whether the urinary kidney injury molecule-1 (uKIM-1) associates with tumor tissue (tKIM-1) expression and with the pathological characteristics of clear renal cell carcinoma (cRCC) in radically nephrectomized (RN) and/or in partially nephrectomized (PN) patients with cRCC, pre- and postoperatively. This clinical study included 40 patients subjected to RN/PN (cRCC group) and 30 healthy volunteers (control group). Urinary KIM-1 was determined by ELISA TIM-1/KIM-1 kit and normalized by urinary creatinine. Immunohistochemical staining (monoclonal anti-human anti-TIM-1/KIM-1/HAVCR antibody) was used for semiquantitative analysis of the tKIM-1 expression and expressed as a score (% KIM-1 positively stained tubules). Both markers were interpreted in terms of the tumor characteristics comprising tumor size, Fuhrman grade, pathological (pT) stage, tumor/nodes/metastasis (TNM) stage, lymphovascular invasion and type of surgery RN/PN. Preoperative uKIM-1 was significantly higher in the cRCC group compared to controls, such as uKIM-1 was statistically higher in RN than in PN patients. Postoperatively, uKIM-1 decreased to control values. Expression of tKIM-1 was documented in all nephrectomized patients. Significant associations were achieved between uKIM-1 and tKIM-1 and with considered tumor characteristics, especially with tumor size and grade. Based on the accomplished associations, we found uKIM-1 as a highly sensitive marker for cRCC diagnosis. The clinical trial registration number: 1110-2012
Advancing Radiation-Detected Resonance Ionization towards Heavier Elements and More Exotic Nuclides
RAdiation-Detected Resonance Ionization Spectroscopy (RADRIS) is a versatile method for highly sensitive laser spectroscopy studies of the heaviest actinides. Most of these nuclides need to be produced at accelerator facilities in fusion-evaporation reactions and are studied immediately after their production and separation from the primary beam due to their short half-lives and low production rates of only a few atoms per second or less. Only recently, the first laser spectroscopic investigation of nobelium (Z=102) was performed by applying the RADRIS technique in a buffer-gas-filled stopping cell at the GSI in Darmstadt, Germany. To expand this technique to other nobelium isotopes and for the search for atomic levels in the heaviest actinide element, lawrencium (Z=103), the sensitivity of the RADRIS setup needed to be further improved. Therefore, a new movable double-detector setup was developed, which enhances the overall efficiency by approximately 65% compared to the previously used single-detector setup. Further development work was performed to enable the study of longer-lived (t1/2>1 h) and shorter-lived nuclides (t1/2<1 s) with the RADRIS method. With a new rotatable multi-detector design, the long-lived isotope 254Fm (t1/2=3.2Ā h) becomes within reach for laser spectroscopy. Upcoming experiments will also tackle the short-lived isotope 251No (t1/2=0.8Ā s) by applying a newly implemented short RADRIS measurement cycle
Opportunities and limitations of in-gas-cell laser spectroscopy of the heaviest elements with RADRIS
International audienceThe radiation detection resonance ionization spectroscopy (RADRIS) technique enables laser spectroscopic investigations of the heaviest elements which are produced in atom-at-a-time quantities from fusion-evaporation reactions. To achieve a high efficiency, laser spectroscopy is performed in a buffer-gas environment used to thermalize and stop the high-energy evaporation residues behind the velocity filter SHIP. The required cyclic measurement procedure in combination with the applied filament collection for neutralization as well as confinement of the stopped ions and subsequent pulse-heat desorption constrains the applicability of the technique. Here, some of these limitations and also opportunities that arise from this unique measurement setup will be evaluated