A Mechanistic Investigation of Allergic Airway Responses and Pulmonary Inflammation in House Dust Mite Sensitized Rats

House dust mite(HDM) antigen is one of the most common allergens associated with extrinsic asthma. In a model of allergic lung disease, Brown Norway rats sensitized to HDM with alum and Bordetella pertussis adjuvants produce high levels of IgE antibody and experience bronchoconstriction, increased airway hyperreactivity, and pulmonary inflammation after antigen challenge. To determine whether this allergic state could be transferred from sensitized animals to naive recipients via humoral or cellular factors, syngeneic recipient rats were injected i.p. with 4X10(superscript)7 cells(pre-cultured overnight with either HDM or BSA, respectively) from lymph nodes of sensitized or control rats. Other groups received a tail vein injection of serum from either HDM-sensitized or control rats. Three days post-transfer, animals were placed in a Fenn plethysmograph to assess bronchoconstriction after antigen challenge. The next day pulmonary hyperreactivity was measured during I.V.infusion of acetylcholine. Pulmonary inflammation was then assessed in bronchoalveolar lavage(BAL) fluid and histopathological sections. Antigen challenge in rats injected with sensitized cells caused increases in pulmonary inflammation and increases in airway hyperreactivity, but no changes in immediate bronchoconstriction compared to control recipients. Serum transferred immediate bronchoconstriction but had no effect on airway hyperreactivity or on pulmonary inflammation. These data demonstrate that immune-mediated lung inflammation and airway hyperreactivity are promoted by antigen-specific cells, while immediate allergic responses are caused by serum factors. Future studies will focus on the isolation and subsequent transfer of serum factors and cell phenotypes to determine which specific components contribute to the pathophysiology of allergic lung disease.Master of Science in Public Healt

Quantitative Comparison of Proprietary and Open-Source Georeferencing Tools for Use with Astronaut Photography

The Crew Earth Observations (CEO) Facility within the Earth Science and Remote Sensing Unit at NASAs Johnson Space Center supports the acquisition, analysis, and curation of astronaut photography of Earths surface and atmosphere. Astronauts on the International Space Station (ISS) respond to requests from CEO to acquire imagery of scientific and education targets, to include high profile targets in response to activations from the International Charter for Space & Major Disasters (also known as the International Disaster Charter, or IDC) and NASAs Disasters Program. CEO facilitates the acquisition of astronaut photography in response to IDC events and delivers georeferenced data products to the United States Geological Survey (USGS) for distribution to the disaster community. Using GeoRef, an internal web-based tool developed in collaboration with NASAs Ames Research Center, CEO generates data packages of georeferenced imagery, uncertainty images for assessing control and tie point accuracy, and metadata documenting raw and processed data. Operational experience with the Georef software identified vulnerabilities to internal code and server errors that can significantly increase time of data production. As such, CEO developed a backup procedure in case the GeoRef software experiences front-end or back-end errors. A system using OSGEOs open-source QGIS software combined with a semi-automated pipeline using the object-oriented Python language and the Geospatial Abstract Library for generating metadata is quantitatively compared to GeoRefs data package for quality and productivity. Root Mean Square Error (RMSE) provides a standard measurement of data quality as it relates to ground error. Assessing RMSE measurements generated from georeferenced astronaut photographs acquired with different obliquity and focal length offers a comprehensive accuracy assessment of the softwares transformation algorithms. This assessment will indicate the software's ability to produce data products with the least ground-error or highest data quality regarding ground accuracy. In addition, a comparison of the softwares efficiency in generating a data package that includes georeferenced images, metadata, and uncertainty images for measuring tie/ground point error was performed. Initial results, based on the comparison of three nadir-facing astronaut photographs acquired with a 95mm focal length, reveal the QGIS-based system's average RMSE is 2.36 (pixels) suggesting its georectification system produces data products that meet and perhaps improve upon Georef solution's average RMSE of 32.99 (pixels). However, the QGIS system was unable to reproduce two unique Georef data products, uncertainty images for measuring tie and control point errors and a translated unwrapped image. In addition, the Georef software is designed to accept handheld camera pose information from a hardware component (Geosens) scheduled for deployment on the ISS in late 2018; this information is intended to provide increased accuracy and auto-registration capability for astronaut photographs. Future work is expected to determine the QGIS-based georectification systems potential as an open-source alternative (and operational backup) to Georef for georeferencing the full range of resolutions and viewing angles unique to handheld digital camera imagery in support of ISS disaster response activities

Examining Change in Therapeutic Alliance to Predict Youth Mental Health Outcomes

Objective—To examine the link between therapeutic alliance and youth outcomes. Method—The study was conducted at a group-home with 112 youth with a disruptive-behavior diagnosis. Therapeutic alliance was collected routinely via youth and staff report. Outcome data were collected using youth and staff reports of externalizing behavior as well as behavioral incidents occurring during care. Outcome data were collected following intake into services and at 6 and 12 months of care. Data were analyzed to examine (1) if youth behavior problems at intake were predictive of therapeutic alliance and (2) if changes in alliance were predictive of subsequent youth outcomes. These were conducted with a 6-month service-delivery model and replicated with a 12-month model. Results—There was some support for the first hypothesis, that initial levels of youth externalizing behavior would be related to alliance ratings; however, most of the effects were marginally significant. The second hypothesis, that changes in therapeutic alliance would be related to subsequent youth outcomes, was supported for the 6-month model, but not the 12-month model. Conclusions—Changes in therapeutic alliance may be predictive of youth outcomes during care. Additional research into examining therapeutic alliance trajectories is warranted to improve mental health services for youth

Measurement Properties of the Motivation for Youth Treatment Scale with a Residential Group Home Population

A client’s motivation to receive services has long been identified as a highly relevant component of mental health treatment. In fact, ample evidence demonstrates that client motivation is significantly related to seeking services, remaining in services, and improved client outcomes (e.g., Broome, Joe, & Simpson, 2001; Ryan, Plant, & O’Malley, 1995; Schroder, Sellman, Frampton, & Deering, 2009). Additionally, it has been recognized that motivation is a “dynamic” characteristic that changes throughout treatment (Melnick, De Leon, Hawke, Jainchill, & Kressel, 1997; Schroder et al., 2009). In this way, motivation is an important client factor to assess and monitor throughout the treatment process. The broad construct of motivation is comprised of two separate, but related components conceptualized as motivation to change and motivation for treatment. As defined by DiClamente, Schlundt, and Gemmell (2004), motivation to change refers to a willingness to recognize problematic behavior and take steps toward change, whereas motivation for treatment refers to a willingness to seek help and remain compliant with an intervention program. In other words, a motivated person not only perceives the importance of changing, but also has confidence that they are able to be successful at making the change (Burke, Arkowitz, & Menchola, 2003)

Validation of the Symptoms and Functioning Severity Scale in Residential Group Care

Tests that measure the emotional and behavioral problems of children and youth are typically not normed and standardized on youth diagnosed with disruptive behavior, particularly those youth in residential care. Yet professional standards mandate that before instruments are used with a specific population the psychometric properties need to be studied and re-established: specifically, psychometric properties, including validity, need to be evaluated (AERA, APA, & NCME, 1999). The purpose of the present study was to assess the validity characteristics of the Symptoms and Functioning Severity Scale (SFSS; Bickman, et al., 2010), a widely used test developed for use in outpatient clinics, with youth in a residential care program. The convergent validity of the SFSS was established with the large correlations (.78-.86) with the CBCL. Several binary classification analyses including specificity, area under the receiver operating characteristic curve, positive and negative likelihood ratios, and the Youden Index supported the validity of the SFSS. However, the sensitivity index was somewhat low indicating the test may produce a high level of false negatives. Limitations, future research and implications are discussed

Psychometric Evaluation of the Symptoms and Functioning Severity Scale (SFSS) Short Forms with Out-of-Home Care Youth

BACKGROUND—There is a need for brief progress monitoring measures of behavioral and emotional symptoms for youth in out-of-home care. The Symptoms and Functioning Severity Scale (SFSS; Bickman et al., 2010) is one measure that has clinician and youth short forms (SFSS-SFs); however, the psychometric soundness of the SFSS-SFs with youth in out-of-home care has yet to be examined. OBJECTIVE—The objective was to determine if the psychometric characteristics of the clinician and youth SFSS-SFs are viable for use in out-of-home care programs. METHODS—The participants included 143 youth receiving residential treatment and 52 direct care residential staff. The current study assessed internal consistency and alternate forms reliability for SFSS-SFs for youth in a residential care setting. Further, a binary classification test was completed to determine if the SFSS-SFs similarly classified youth as the SFSS full version for low- and elevated-severity. RESULTS—The internal consistency for the clinician and youth SFSS-SFs was adequate (α = .75 to .82) as was the parallel forms reliability (r = .85 to .97). The sensitivity (0.80 to 0.95), specificity (0.88 to 0.97), and overall accuracy (0.89 to 0.93) for differentiating low and elevated symptom severity was acceptable. CONCLUSIONS—The clinician and youth SFSS-SFs have acceptable psychometrics and may be beneficial for progress monitoring and additional research should clarify their potential for progress monitoring of youth in out-of-home programs

Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007

One-sentence summary for table of contents: The full coding region nucleic acid sequence and serologic properties of the virus were identified

Differential effects of RASA3 mutations on hematopoiesis are profoundly influenced by genetic background and molecular variant.

Studies of the severely pancytopenic scat mouse model first demonstrated the crucial role of RASA3, a dual RAS and RAP GTPase activating protein (GAP), in hematopoiesis. RASA3 is required for survival in utero; germline deletion is lethal at E12.5-13.5 due to severe hemorrhage. Here, conditional deletion in hematopoietic stem and progenitor cells (HSPCs) using Vav-iCre recapitulates the null phenotype demonstrating that RASA3 is required at the stem and progenitor level to maintain blood vessel development and integrity and effective blood production. In adults, bone marrow blood cell production and spleen stress erythropoiesis are suppressed significantly upon induction of RASA3 deficiency, leading to pancytopenia and death within two weeks. Notably, RASA3 missense mutations in two mouse models, scat (G125V) and hlb381 (H794L), show dramatically different hematopoietic consequences specific to both genetic background and molecular variant. The mutation effect is mediated at least in part by differential effects on RAS and RAP activation. In addition, we show that the role of RASA3 is conserved during human terminal erythropoiesis, highlighting a potential function for the RASA3-RAS axis in disordered erythropoiesis in humans. Finally, global transcriptomic studies in scat suggest potential targets to ameliorate disease progression

Socio-eco-evolutionary dynamics in cities

Cities are uniquely complex systems regulated by interactions and feedbacks between nature and human society. Characteristics of human society-including culture, economics, technology and politics-underlie social patterns and activity, creating a heterogeneous environment that can influence and be influenced by both ecological and evolutionary processes. Increasing research on urban ecology and evolutionary biology has coincided with growing interest in eco-evolutionary dynamics, which encompasses the interactions and reciprocal feedbacks between evolution and ecology. Research on both urban evolutionary biology and eco-evolutionary dynamics frequently focuses on contemporary evolution of species that have potentially substantial ecological-and even social-significance. Still, little work fully integrates urban evolutionary biology and eco-evolutionary dynamics, and rarely do researchers in either of these fields fully consider the role of human social patterns and processes. Because cities are fundamentally regulated by human activities, are inherently interconnected and are frequently undergoing social and economic transformation, they represent an opportunity for ecologists and evolutionary biologists to study urban "socio-eco-evolutionary dynamics." Through this new framework, we encourage researchers of urban ecology and evolution to fully integrate human social drivers and feedbacks to increase understanding and conservation of ecosystems, their functions and their contributions to people within and outside cities