Actualizing hope and joy in K-12 curricula through Daisaku Ikeda’s human education

Purpose: Our study aims to create a framework grounded in Daisaku Ikeda’s philosophy of education for educators and researchers to implement and evaluate human education in the classroom. Research Methodology: We first synthesized the eighteen chapters by scholars involved in Ikeda studies, published in the book entitled: Hope and Joy in Education: Engaging Daisaku Ikeda across Curriculum and Context to discover the main themes in Ikeda’s human education. Based on these themes, we selected six children’s stories developed by Ikeda to design lessons. We then conducted surveys with ten K-12 teachers across disciplines and school districts to explore their perspectives toward humanity and their feedback on our lesson design. Results: Our finding indicates that creating hope and joy in education is inseparable from human revolution, value creation, happiness, the greater self, global citizens, as well as life and death. In addition, the participant’s responses to the survey questions help educators and researchers understand what K-12 teachers look for in order to implement lessons on humanity more efficiently and effectively. Limitations: By no means would we consider our lesson design exemplary or applicable in all different contexts. Instead, we consider these lessons a starting point to continue exploring a better way to teach humanity in school. Contribution: Seeing examples of lesson plans on humanity and learning from K-12 teachers’ perspectives provide an aspect for educators and researchers to use, extend, or expand the present study to bring hope and joy to students in their local contexts

Design and Construction of Drilled Shafts in Karst Environments of Florida

Drilled shafts have been widely used as bridge foundation alternatives for more than a decade in Florida. The majority of the drilled shafts are designed to embed into the underlying limestone. However, many unforeseen conditions have been encountered during the construction of drilled shafts due to karst environments, especially in the Tampa Bay area where sinkhole occurrences are common. This paper presents a case history of the design and construction of drilled shaft foundations for the I-4/I-275 Downtown Interchange in Tampa, Florida. A two-phase procedure utilized by the Florida Department of Transportation (FDOT) was adopted to minimize the impact of karst environments on drilled shaft construction and contractors’ claims, while also considering the project schedule and budget. A total of 315 drilled shafts with total lengths of 3,914 meters were installed for this project. Although the estimated total drilled shaft lengths in the preliminary design phase were only underestimated by 10%, high variability of individual shaft lengths between those estimated during the preliminary and final designs were observed with a maximum difference up to 20 m. The evaluation of the impacts of the karst environments on the drilled shaft design, and the comparison and discussion of the drilled shaft lengths determined during design and as-built are presented

Prevalence of Mental Health Disorder Symptoms and Rates of Help-seeking Among University-Enrolled, Black Men

Background. Black men in college represent a subgroup of emerging adults who are at increased risk of developing mental health disorders (MHDs), such as anxiety and depression. Such risk has been attributed to disproportionate experiences with everyday racial discrimination and high levels of psychological distress. Despite being at higher risk, university-enrolled, Black men are not utilizing mental health or health resources at optimal rates. The current evidence base describing prevalence of MHDs and health services utilization among Black men in college is limited. The present study addresses this by examining mental health prevalence among university-enrolled, Black men and their rates of health services utilization. Methods. We analyzed data (N ~ 2500) from a student survey, Spit for Science, a longitudinal, ongoing, research study at a mid-Atlantic, public university. Participants are given surveys in their freshman year and follow-up surveys every spring thereafter. Measures included: mental health disorders (depression and anxiety, as measured by the Symptom Checklist 90) and campus health service utilization (counseling center, health services, wellness center, and recreational sports). We conducted descriptive analyses to determine MHD symptom prevalence and utilization rates; Mann Whitney U tests to compare prevalence rates to White men and Black women; and, Chi-squared tests to compare rates of utilization among groups. Results. During their Freshman year, greater than 60% of students from each ethnic group reported at least one anxiety symptom and greater than 80% reported at least one depressive symptom. By senior year, reporting rates decreased significantly for Black men (49.6%) but remained high for White men (69.1%) and Black women (63%); p \u3c0.000. For depression, results were similar; however, only significant differences between Black men (72.7%) and Black women (87.1%); p\u3c0.000. Black men (20.4%), though reporting high levels of symptoms, still utilized counseling services at lower rates compared to White men (37.76%); p = 0.024. Conclusion. Findings suggest that Black men underutilize available campus health resources despite reporting one or more symptoms associated with anxiety and depression. Further research and prevention efforts are needed to improve help-seeking among this vulnerable population.https://scholarscompass.vcu.edu/gradposters/1077/thumbnail.jp

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

In this report we present a method to cultivate Borrelia spirochetes from human serum samples with high efficiency. This method incorporates improved sample collection, optimization of culture media and use of matrix protein. The method was first optimized utilizing Borrelia laboratory strains, and later by demonstrating growth of Borrelia from sera from fifty seropositive Lyme disease patients followed by another cohort of 72 Lyme disease patients, all of whom satisfied the strict CDC surveillance case definition for Lyme disease. The procedure resulted in positive cultures in 47% at 6 days and 94% at week 16. Negative controls included 48 cases. The positive identification of Borrelia was performed by immunostaining, PCR, and direct DNA sequencing

Family-Centered Care: Current Applications and Future Directions in Pediatric Health Care

Family-centered care (FCC) is a partnership approach to health care decision-making between the family and health care provider. FCC is considered the standard of pediatric health care by many clinical practices, hospitals, and health care groups. Despite widespread endorsement, FCC continues to be insufficiently implemented into clinical practice. In this paper we enumerate the core principles of FCC in pediatric health care, describe recent advances applying FCC principles to clinical practice, and propose an agenda for practitioners, hospitals, and health care groups to translate FCC into improved health outcomes, health care delivery, and health care system transformation

Toolkit for Service Learning. Building Community Relationships and Partnerships

I first met Amy Kuo Somchanhmavong and Shorna Allred at the International Conference on Engaged Learning and Information and Communication Technologies (ICTs) for Development in the University Curriculum. It was co-organized by Cornell University, the University of Selangor and the Asian Institute for Development Communication, and held in Selangor, Malaysia from 3_5 June 2014. Amy and Shorna presented their service learning experiences working with rural communities in Asia while I outlined my experiences with UNIMAS from introducing ICTs to remote communities in Malaysian Borneo. We clicked immediately. After boldly suggesting to Amy and Shorna to consider linking their programme to ours, they displayed admirable courage and creativity to take the idea seriously, with the result that, after a great deal of meticulous planning, preparation and logistical co-ordination, the first cohort of Cornell- UNIMAS service learning students, which included Rachel Whalen, arrived in the remote and isolated community of Long Lamai on Borneo in January 2016. I had the good fortune to be invited to join them on that groundbreaking initiative, which has since blossomed into many more such student community encounters and a fully-fledged campus-wide service learning programme at UNIMAS. In addition to Amy and Shorna’s professionalism and dedication, what struck me most was how well the students adapted to the totally alien environment of Borneo’s interior, and, equally critical, how well the community warmed to their presence. Without doubt, these outcomes were in no small part due to the assiduous development and implementation of the processes outlined in this book. My experiences have taught me that cross-cultural community development encounters are laden with pitfalls for the unprepared. Had I had access to the accumulated wisdom and advice here, my own efforts would have been greatly eased. Anyone engaged in service learning needs practical advice from people with direct experience who are drawn from the entire range of relevant stakeholders. The service learning programme that is featured here has spawned a broad catalogue of highly desirable outcomes. The participating students from Cornell are engaged within the University’s Global Citizenship and Sustainability Program which fosters cross-cultural learning while building skills in community-based research. The feedback I have received from them suggests it does far more than this; engendering a life-changing outlook on the world that they will carry forward as tomorrow’s leaders into whatever occupation they adopt. The communities benefit from their joint participation through the injection of external perspectives on localised development schemes that strengthen resilience and promote sustainable livelihoods. UNIMAS has absorbed its learning from the engagement with Cornell and has now integrated service learning into every academic programme that it offers. Finally, arising from its origin within another programme for deploying ICTs among rural communities, thereby adding social and economic value to the technology, the approach to Service learning that is described here, involving local managers of community internet centres, achieves the additional benefit of helping to achieve the full potential of digital technology for citizens who have the least access to it. Service learning allows students to engage with communities in order to apply the knowledge and skills that they have learned in the classroom to their needs. This service learning Toolkit will show you how to do this. Roger Harri

Attitudes and Opinions About Direct-to-Consumer Genetic Testing in Undergraduate Science Students

Background: There has been exponential growth in the number of direct-to-consumer genetic testing kits sold in the past decade. Consumers utilize direct-to-consumer genetic tests for a number of reasons which include learning about one’s ancestry and potential ways to manage health. Emerging adults tend to be early adopters of new technologies; however, there has been little research regarding the opinions about direct-to-consumer genetic testing in emerging adults. Methods: Data came from a study conducted in an upper-level biology course focusing on understanding undergraduate science students’ overall experiences with receiving personalized genetic testing results from 23andMe. The present study used data collected at the baseline assessment which assessed their opinions and attitudes about direct-to-consumer genetic testing (N=133). Results: Over 80% of participants would recommend direct-to-consumer genetic testing options including carrier status reports, DNA ancestry reports, wellness reports, and trait reports to others. However, participants were not as confident that others would be able to accurately interpret their test results. Additionally, more than two-thirds of the participants stated that they would ask a healthcare provider to help interpret their personalized genetic test results. Conclusions: Participants lack confidence in both their ability to interpret their own results and others to interpret their results. It is important for direct-to-consumer genetic testing companies to educate consumers before providing results in order to minimize potential harms due to misinterpretation of results. Further research is needed to assess motivations to participate in direct-to-consumer genetic testing, impact of testing, and understanding of genetic testing results in emerging adults.https://scholarscompass.vcu.edu/gradposters/1124/thumbnail.jp

Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana

During meiosis, DNA double-strand breaks (DSBs) occur throughout the genome, a subset of which are repaired to form reciprocal crossovers between chromosomes. Crossovers are essential to ensure balanced chromosome segregation and to create new combinations of genetic variation. Meiotic DSBs are formed by a topoisomerase-VI-like complex, containing catalytic (e.g. SPO11) proteins and auxiliary (e.g. PRD3) proteins. Meiotic DSBs are formed in chromatin loops tethered to a linear chromosome axis, but the interrelationship between DSB-promoting factors and the axis is not fully understood. Here, we study the localisation of SPO11-1 and PRD3 during meiosis, and investigate their respective functions in relation to the chromosome axis. Using immunocytogenetics, we observed that the localisation of SPO11-1 overlaps relatively weakly with the chromosome axis and RAD51, a marker of meiotic DSBs, and that SPO11-1 recruitment to chromatin is genetically independent of the axis. In contrast, PRD3 localisation correlates more strongly with RAD51 and the chromosome axis. This indicates that PRD3 likely forms a functional link between SPO11-1 and the chromosome axis to promote meiotic DSB formation. We also uncovered a new function of SPO11-1 in the nucleation of the synaptonemal complex protein ZYP1. We demonstrate that chromosome co-alignment associated with ZYP1 deposition can occur in the absence of DSBs, and is dependent on SPO11-1, but not PRD3. Lastly, we show that the progression of meiosis is influenced by the presence of aberrant chromosomal connections, but not by the absence of DSBs or synapsis. Altogether, our study provides mechanistic insights into the control of meiotic DSB formation and reveals diverse functional interactions between SPO11-1, PRD3 and the chromosome axis

3',5'-Cyclic Adenosine Monophosphate- and Ca2+-Calmodulin-Dependent Endogenous Protein Phosphorylation Activity in Membranes of the Bovine Chromaffin Secretory Vesicles: Identification of Two Phosphorylated Components as Tyrosine Hydroxylase and Protein Kinase Regulatory Subunit Type II

Abstract: Membranes of the secretory vesicles from bovine adrenal medulla were investigated for the presence of the endogenous protein phosphorylation activity. Seven phosphoprotein bands in the molecular weight range of 250,000 to 30,000 were observed by means of the sodium dodecyl sulphate electrophoresis and autoradiography. On the basis of the criteria of molecular weight, selective stimulation of the phosphorylation by cyclic AMP (as compared with cyclic GMP) and immunoprecipitation by specific antibodies, band 5 (molecular weight 60,300) was found to represent the phosphorylated form of the secretory vesicle-bound tyrosine hydroxylase. The electrophoretic mobility, the stimulatory and inhibitory effects of cyclic AMP in presence of Mg2+ and Zn,2+ respectively, and immunoreactivity toward antibodies showed band 6 to contain two forms of the regulatory subunits of the type II cyclic AMP-dependent protein kinase, distinguishable by their molecular weights (56,000 and 52,000, respectively). Phosphorylation of band 7 (molecular weight 29,800) was stimulated about 2 to 3 times by Ca2+ and calmodulin in the concentration range of both agents believed to occur in the secretory tissues under physiological conditions