The surface treatment of dental ceramics: an overview

This review proposes to present some aspects of dental ceramics surface treatments, which involve the related physical and mechanical properties, as well as new technologies. The correct cementation of prosthetic restoration is essential for clinical success. However, the adhesive technique is a very recent procedure and clinicians should have a more detailed knowledge about the technique and related materials so that they can use them in their day-to-day clinical practice. Information about the the principles, indications and contraindications of adhesive cementation technique were obtained from the literature. The studies indicate that the adhesive system seems to be a valid option for fixed prosthesis cementation, since it has several advantages over conventional cementation method, especially in all-ceramic restorations. In clinical practice, the clinician should always keep in mind the characteristics of the material they are working with, so that they can select the best technique and the best cement for each clinical situation. Although more research is required in this field, there are no scientific reasons to contraindicate in routine practice

Histological and immunohistochemical analysis of a nanobiomaterial in a maxillary sinus lift surgery: a case report

The objective of this study was to histologically and immunologically analyze the level of bone substitution and the presence of new blood vessels in a nanobiomaterial composed of hydroxyapatite and BTCP in a maxillary sinus lift surgery. A case of a maxillary sinus lift was investigated. The patient had a 1.0 mm bone remnant on the left side and 2 grams of nanobiomaterial (80% hydroxyapatite and 20% β-tricalcium phosphate) were grafted into the pneumatized sinus. After 6 months, during dental implant placement surgery, grafted bone samples were collected with 4 mm trephines. These samples were sent to the laboratory for analysis where they were stained with masson'strichrome and immuno stained with osteonectin and osteopontin. After 6 months of bone regeneration a result of 14 mm of bone gain was reached, the analyzes in masson's trichrome showed an excellent gain of newly formed bone, in addition to a very high percentage of blood vessels. In the immunostaining, a very large number of osteoblasts and osteoclasts was observed, signaling an excellent osteoconduction and osteoinduction of the studied nanobiomaterial. The maxillary sinus lift surgery with nanobiomaterial provided a very favorable bone height and thickness gain, as well as the high vascularity and cellularity, which enabled the patient's oral rehabilitation with osseointegrated dental implants and permanent ceramic prostheses.info:eu-repo/semantics/publishedVersio

Sedentary behavior : a key component in the interaction between an integrated lifestyle approach and cardiac autonomic function in active young men

This study aimed to verify the association between autonomic cardiac function (CAF) and the integration of caloric expenditure by physical activity (PA) intensity, sedentary behavior (SB), and sleep quality (PSQI) in active young men. Thirty-five subjects were included, and caloric expenditure in moderate-to-vigorous and light-intensity PA, SB, and PSQI were assessed using questionnaires. Heart rate variability (HRV) was recorded for short periods of time in the supine and orthostatic positions. Multiple linear regression was realized unadjusted and adjusted for covariables, such as age, body mass index, and fat mass. No adjusted analysis indicated that, in the supine position, there were negative associations between the SB and the TP, HF, and NorHF indices, and positive associations between SB and NorLF and LF/HF. In the orthostatic position, an interaction between SB and NorLF was found. Significance of proportion with the TP, HF, and LF/HF indices was confirmed. When adjusted, for the supine position, negative interactions were documented between SB and the TP as well as the HF indices, and between PSQI and the LF/HF index, with interference under the HF and LF/HF indices. Finally, our findings indicate that the proposed approach interacts with CAF, and SB is significantly related to CAF in young active men

Períodos de interferência de plantas daninhas na cultura do crambe cultivado em Rio Verde, GO

Objetivou-se neste trabalho identificar os períodos de interferência de plantas daninhas na cultura do crambe (Crambe abyssinica Hochst) cultivado em Rio Verde, GO. Dois ensaios foram conduzidos simultaneamente sendo que no primeiro a cultura permaneceu livre da competição com plantas daninhas desde a emergência até os 0; 7; 14; 21; 28; 35; 42; 49 e 89 dias. No segundo a cultura permaneceu em competição com a comunidade infestante pelos mesmos períodos citados no primeiro ensaio. O delineamento utilizado foi de blocos ao acaso, com quatro repetições. A espécie daninha Alternanthera tenella foi a de maior importância devido as altas frequencias, densidades e dominância relativas. A massa de mil grãos de crambe não foi afetada pela interferência da comunidade infestante. O rendimento de grãos do crambe foi afetado pela convivência com as plantas daninhas. O período anterior à interferência (PAI) foi desde a emergência até 37 dias após a emergência e o período total de prevenção à interferência (PTPI) foi da emergência até 60 DAE. O período crítico de prevenção da interferência situou-se entre os 37 e 60 DAE. Neste período até 60 DAE a convivência das plantas daninhas com o crambe ocasionou diminuição no rendimento da cultura por competição pelos recursos do meio

Derivação biliodigestiva por estenose biliar tardia complicada com hemorragia digestiva alta maciça por fístula artérioentérica - um relato de caso e revisão da literatura

Este estudo de caso documenta a evolução clínica de uma paciente do sexo feminino de 54 anos, que apresentou patologia biliar complexa levando a múltiplas intervenções cirúrgicas e subsequentes complicações pós-operatórias. Dois anos depois, apresentou novamente sintomas agudos e os exames de imagem indicaram dilatação das vias biliares, levando ao diagnóstico de Colangite. A colangiopancreatografia retrógrada endoscópica (CPRE) foi tentada, mas sem sucesso, necessitando de drenagem biliar transparieto-hepática. Posteriormente, o paciente foi submetido a uma hepaticojejunostomia laparoscópica em Y-de-Roux para uma estenose biliar de Strassberg E2. O paciente apresentou hemorragia gastrointestinal grave, levando a choque hipovolêmico. Apesar da ausência de fonte de sangramento na endoscopia digestiva alta e na angiotomografia da aorta abdominal, suspeitou-se de hemorragia no membro biliopancreático. Foi realizada embolização superseletiva da artéria hepática direita. Após cuidados intensivos e múltiplas transfusões de sangue com eventual alta. Este caso ressalta as complexidades envolvidas no manejo de doenças graves do trato biliar e suas complicações, destaca a necessidade de uma abordagem abrangente e multidisciplinar para um tratamento e recuperação eficazes

Pumpkin seeds (Cucurbita moschata - Jacarezinho cultivar): characterization of the oil extracted by solvent and supercritical fluid and study of anti-parasitary activity / Sementes de abóbora (Cucurbita moschata - cultivar Jacarezinho): caracterização do óleo extraído por solvente e fluido supercrítico e estudo da atividade antiparasitária

The presence of many biologically active components makes pumpkins extremely attractive to the phytochemical manufacturing industry. Studies have demonstrated that the oil extracted from the seeds has different biological activities. This study aimed to determine the fatty acid composition and total tocopherol content of the pumpkin seed oil (Cucurbita moschata – Jacarezinho cultivar) extracted by supercritical carbon dioxide (Sc-CO2) and hexane/isopropanol. The fatty acids composition and content of total tocopherols were determined by GC, GC/FID, HPLC, respectively. We also evaluated in vitro schistosomicidal activities of the crude oil, which have been described with anti-parasitic activities. Sc-CO2 extracted pumpkin seed oil, with a maximum yield of 24.3 ± 0.4%, much higher than hexane/isopropanol extraction (8.3 ± 2.7%). Was not observe differences between the non-polar compounds present in the oil extracted by both methods. In the seed oil, unsaturated acids are dominant (oleic and linoleic). The results indicate that the oil has an excellent quality, with high contents of unsaturated fatty acids (73% of total fatty acids) and total tocopherols (14 mg100g-1 of oil). Although popularly reported as anti-parasitic activities caused no mortality, tegumental alterations or significant decrease in motor activity in all adult parasites. Sc-CO2 was able to extract pumpkin seed oil with a much higher yield than extraction with hexane/isopropanol and no differences were observe between the non-polar compounds present in the oil extracted by both methods. The oil is popularly used as an antiparasitic agent but in this study did not show antiparasitary actividies

Transcriptomic landscape of skin lesions in cutaneous leishmaniasis reveals a strong CD8(+) T cell immunosenescence signature linked to immunopathology

The severity of lesions that develop in patients infected by Leishmania braziliensis is mainly associated with a highly cytotoxic and inflammatory cutaneous environment. Recently, we demonstrated that senescent T and NK cells play a role in the establishment and maintenance of this tissue inflammation. Here, we extended those findings using transcriptomic analyses that demonstrate a strong co-induction of senescence and pro-inflammatory gene signatures in cutaneous leishmaniasis (CL) lesions. The senescence-associated signature was characterized by marked expression of key genes such as ATM, Sestrin 2, p16, p21 and p38. The cell type identification from deconvolution of bulk sequencing data showed that the senescence signature was linked with CD8+ effector memory and TEMRA subsets and also senescent NK cells. A key observation was that the senescence markers in the skin lesions are age-independent of patients and were correlated with lesion size. Moreover, a striking expression of the senescence-associated secretory phenotype (SASP), pro-inflammatory cytokine and chemokines genes was found within lesions that were most strongly associated with the senescent CD8 TEMRA subset. Collectively, our results confirm that there is a senescence transcriptomic signature in CL lesions and supports the hypothesis that lesional senescent cells have a major role in mediating immunopathology of the disease

A total transcriptome profiling method for plasma-derived extracellular vesicles: applications for liquid biopsies

Extracellular vesicles (EVs) are key mediators of intercellular communication. Part of their biological effects can be attributed to the transfer of cargos of diverse types of RNAs, which are promising diagnostic and prognostic biomarkers. EVs found in human biofluids are a valuable source for the development of minimally invasive assays. However, the total transcriptional landscape of EVs is still largely unknown. Here we develop a new method for total transcriptome profiling of plasma-derived EVs by next generation sequencing (NGS) from limited quantities of patient-derived clinical samples, which enables the unbiased characterization of the complete RNA cargo, including both small- and long-RNAs, in a single library preparation step. This approach was applied to RNA extracted from EVs isolated by ultracentrifugation from the plasma of five healthy volunteers. Among the most abundant RNAs identified we found small RNAs such as tRNAs, miRNAs and miscellaneous RNAs, which have largely unknown functions. We also identified protein-coding and long noncoding transcripts, as well as circular RNA species that were also experimentally validated. This method enables, for the first time, the full spectrum of transcriptome data to be obtained from minute patient-derived samples, and will therefore potentially allow the identification of cell-to-cell communication mechanisms and biomarkers.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Gillson-Longenbaugh FoundationNational Institutes of Health (NIH/NCATS) through the NIH Common Fund, Office of Strategic Coordination (OSC)AC Camargo Canc Ctr, Lab Med Genom, Sao Paulo, SP, BrazilAC Camargo Canc Ctr, Lab Computat Biol, Sao Paulo, SP, BrazilUniv Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Electron Microscopy Ctr, Sao Paulo, SP, BrazilUniv Texas MD Anderson Canc Ctr, Dept Expt Therapeut, Houston, TX 77030 USAUniv Texas MD Anderson Canc Ctr, Ctr RNA Interference & Non Coding RNAs, Houston, TX 77030 USAUniv New Mexico, Comprehens Canc Ctr, Albuquerque, NM 87131 USAUniv New Mexico, Sch Med, Div Hematol Oncol, Dept Internal Med, Albuquerque, NM 87131 USAUniv New Mexico, Sch Med, Div Mol Med, Dept Internal Med, Albuquerque, NM 87131 USARockefeller Univ, Lab Mol Immunol, 1230 York Ave, New York, NY 10021 USAFMUSP, Lab Neurociencias Alzira Denise Hertzog Silva LIM, Inst Psiquiatria, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Electron Microscopy Ctr, Sao Paulo, SP, BrazilFAPESP: 2011/09172-3FAPESP: 2014/26897-0Web of Scienc

Uma avaliação sistemática sobre os efeitos de metais e metaloides, presentes em sedimentos, sobre comunidades de macroinvertebrados bentônicos em estuário subtropical no Brasil – sistema estuarino de Paranaguá

A população mundial encontra-se localizada principalmente em áreas costeiras, especialmente nas áreas de baías e estuários por conta de suas localizações estratégicas, abrigando instalações de atividades industriais, marítimas e de veraneio, bem como servindo como fonte de alimentos. Considerando este aspecto, os estuários e baías tornam-se receptores de diferentes elementos de origem antrópica oriundos da drenagem urbana, de efluentes industriais, despejos de embarcações e fontes pontuais poluidoras, vindas do continente. Sob a perspectiva global, os elementos traços são considerados contaminantes críticos para os ambientes costeiros. Dependendo do contaminante, da sua propriedade físico-química e das condições do meio o contaminante pode se manter na água, ser adsorvido no sedimento ou absorvido por organismos. Devido à baixa mobilidade da maioria dos organismos bentônicos, a presença de elementos traços nos sedimentos pode ser mais prejudicial para as comunidades de macroinvertebrados bentônicos do que para outras comunidades do ecossistema aquático. No presente estudo para caracterizar a distribuição de elementos traços e macroinvertebrados bentônicos e avaliar a relação entre a contaminação por metais e as estruturas das comunidades bentônicas, coletou-se 16 amostras superficiais no sistema estuarino de Paranaguá, abrangendo séries temporais ao longo do ano de 2018. Foram avaliadas também as características geoquímicas dos sedimentos (tamanho de partículas, nutrientes e matéria orgânica), parâmetros físico-químicos da coluna d’água (salinidade, pH, temperatura, OD e turbidez), a concentrações de metais em sedimentos, a diversidade (índice de Shannon), a riqueza e abundância das espécies identificadas. Nesse contexto os resultados indicaram que a concentração média dos elementos traços nos sedimentos durante o período seco seguiu a seguinte ordem: Zn > Cr > Cu > Pb > Ni > As > Cd. Já no período chuvoso a concentração média decresceu da seguinte forma: Zn > Cr > Pb > Cu > Ni > As > Cd.  A estrutura das comunidades de macroinvertebrados bentônicos também foi estudada, juntamente com a análise das correlações entre elementos traços e comunidades bentônicas. Foram identificados 12 filos e 12 classes, e os grupos dominantes foram anelídeos e moluscos. Os resultados da correlação entre as métricas da comunidade bentônica e as concentrações de elementos traços indicam que a riqueza e abundância são mais sensíveis à contaminação por elementos traços. As principais influências na comunidade bentônica podem ser o Cd > As > Mn. Os resultados encontrados sugerem que a comunidade bentônica também pode ser utilizada no biomonitoramento de áreas de relevância ambiental, como o Sistema Estuarino de Paranaguá, quanto a contaminação por elementos traços. Assim tem-se mais um balizador que possa orientar a tomada de decisões e atender as necessidades ecológicas e sociais de maneira sustentável