Seroprevalence of Hepatitis B Virus and Human Immunodeficiency Virus infection among students in Ahmadu Bello University, Zaria, Nigeria

Hepatitis B virus (HBV) and Human Immunodeficiency Virus (HIV) are endemic in sub-Saharan Africa. Evidence on HBV co-infection rates with HIV infection among individuals remains conflicting. The study was conducted to determine the seroprevalence of HBV and HIV infections and the possible potential risk factors among students of Ahmadu Bello University (ABU), Zaria, Nigeria. Blood samples were collected from 600 consenting consecutive students aged between 16 and 40 years old at the University Health Services, ABU, Zaria. The sera were screened for HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc using diagnostic kits and for Human Immunodeficiency Virus using DetermineTMHIV-1/2 kits. Reactive sera for HBsAg were further confirmed using ELISA kits. For HBsAg, 9.2% (55/600) tested positive among which, none had detectable anti-HBs antibodies, indicating recent infection. About 7.3%, 36.4% and 94.5% were positive for HBeAg, anti-HBe and anti-HBc respectively. Seroprevalence of HIV infection was 2.8% (17/600). One (0.2%) of the student was infected with both HBV and HIV. There was a significant association between age group (p=0.016), gender (p=0.049), family history of HBV infection (p=0.000), and seroprevalence of HBsAg. While for HIV, only menial jobs (p = 0.001) was significantly associated with the infection. The results showed close contact among family members to be a predisposing factor to these viral infections. A total of 314 students were ignorant of HBV and four of them were infectious. The seroprevalence of HBsAg obtained in this study indicates high endemicity according to WHO classification. However, seroprevalence of HIV and its co-infection rate with HBV were very low. This was encouraging and it indicates that the campaign on HIV is yielding the desired result. Therefore similar campaign should be extended to Hepatitis B.Keywords: Seroprevalence; HIV; HBsAg; Serological markers; Students; Nigeri

Mutagenic effectiveness and efficiency of gamma rays on variability for yield attributing traits in Fonio (Digitaria exilis [Kippist] Stapf.)

A study was conducted to determine the mutagenic effectiveness and efficiency of different doses of gamma rays in inducing variability that could be exploited in the genetic improvement of fonio. Seeds of five accessions of fonio were irradiated with five different doses of gamma rays (100 Gy, 200 Gy, 400 Gy, 500 Gy and 0 Gy as control). The seeds were sown in a plot in a completely randomized design with three replications to rise the M1 generation which was advanced to M2 generation. The result obtained from the M2 mutants revealed highly significant difference (P≤0.01) in the effects of different gamma rays doses on the growth and yield traits of fonio. This implies the presence of high genetic variability induced by the mutagen in fonio. The effect is concentration dependent, increase with decrease in irradiation dose. More so, the effectiveness of the mutagen decreases with increase in dose. Seven different chlorophyll deficient mutants were found in the form of: Albina, Chlorina, Lustescent, Striata, Viridis, Viriscent and Xantha, with Xantha, Chlorina and Albina as the most frequent chimeras, The result indicated that lower dose of gamma rays (100 Gy) is more effective and efficient as it induces favorable mutation that could be utilized in the genetic improvement of fonio. Accession Nkpowas was found to respond more to the mutagenic treatment. Keywords: Accessions, Fonio, Gamma Rays, Mutation

Phytochemical and antimicrobial activity of securidaca longipedunculata root against urinary tract infection pathogens

Urinary tract infection caused by bacteria leads to inflammation and over growth of uropathogens and prevalence of infection for both genders, but women is more vulnerable especially at the sexually active ages. But unfortunately, the continuous emergence of antibiotic resistant bacterial strains brings most serious public health concerns. It is therefore, important to look for more alternative, effective, safer and safer treatments. The aim of the present study was to investigate antimicrobial activity of Securidaca longipedunculata root extracts against some human pathogenic bacteria and fungi using agar well diffusion method and agar dilution for minimum inhibitory concentration (MIC). Phytochemical and acute toxicity studies were carried out using the standard methods. Phytochemicals which include alkaloids, flavonoids, saponins, tannins, carbohydrates and triterpenes were detected in both aqueous and methanolic extracts. The antimicrobial results revealed that, the methanolic extract had promising antibacterial activity. For intense Escherichia coli was found to be the most susceptible bacteria in both methanol and aqueous extracts at 500mg/ml with inhibition zones of 20 mm and 16mm, Staphylococcus aureus was next most susceptible bacteria to methanol extract of the root with inhibition zone of 16 mm and MIC of 31.25 mg/ml respectively. The extracts does not showed activity against all the tested fungal isolates at lowest concentration 62.5 mg/ml. The LD50 of Securidaca longipedunculata was found to be greater than 5000 mg /kg and could be considered safe for consumption

Phytochemical, Nutritional Composition and Heavy Metals Content of Allium cepa (Onion) and Allium sativum (Garlic) from Wudil Central Market, Kano State, Nigeria

Allium plants are common spices used as food ingredients. Allium cepa (onion) and Allium sativum (garlic) are cultivated in Wudil town. In this study the qualitative and quantitative phytochemicals in Allium cepa revealed the presence of alkaloids (6.45mg/kg), tannins (1.25mg/kg), flavonoids (1.51mg/kg), total phenolic (20.68mg/kg) while saponin was absent. On the other hand Allium sativum shows alkaloids (4.68mg/kg), tannin (0.25mg/kg) and saponin (0.44mg/kg). Proximate composition of garlic show (p<0.05) increase in fat (2.82%), carbohydrate (24.55%), fibre (2.74%), and ash (4.22%) when compared with onion, while protein (2.96%) and moisture (87.36%) are (p<0.005) higher in onion. Anti-nutritional factors present in onion show (p<0.05) increase in oxalate (11.85mg/kg) and tannins (0.76mg/kg), while garlic shows (p<0.05) increase in phytate (1.79mg/kg) and hydrogen cyanide (0.39mg/kg). Heavy metals in onion were (p<0.05) higher in Cd, Cu, Fe, Pb, and Mn, while garlic only show (p<0.05) increase in Zn and Co. The Cr in onion shows (p>0.05) difference when compared with garlic. Cd and Pb in onion and garlic were above WHO recommended safe limits in vegetables. From this study it is observed that the agro-climatic condition may have effect on the phytochemicals of both plants samples from Wudil, while sources of the heavy metals need to be investigated and controlled from level of cultivation to that of processing

Pharmacognostic and Acute Toxicity Study of Burkea Africana Root

Burkea africana is a plant that belongs to then family Fabaceae; it is widely spread in tropical Africa including Nigeria. It is of valuable in  ethnomedicine especially in the treatment of antidote for venomous stings and bites, cutaneous and sub cutaneous parasitic infection, convulsion and pulmonary troubles. Despite the fact that roots of Burkea africana have several medicinal properties, no standardization parameter has been  assessed. Due to lack of standard parameters, proper identification and ascertaining quality and purity in the events of adulteration has been thwarted. The objective of the study was to establish some important pharmacognostic profile and safety margin of Burkea africana root with the hope of assisting in its standardization for quality, purity and safety. Elemental analysis was carried out using acid digestion method and phytochemical composition of the plants was evaluated using standard method. Acute toxicity was achieved using Lorke method to determine the LD50. Chemomicroscopical evaluation revealed the presence of cellulose, tannins, starch, lignin, calcium oxalate, suberin, aleurone grain and mucilage with the exception of calcium carbonate. The average moisture contents, total ash, acid insoluble, water soluble ash, alcohol extractive value and water extractive values in the powdered plant material were 3.8%, 7.5%, 4.43%, 8.07%, 25.0% and 20.33% respectively. In addition, Fe, Mn, Ni, Pb, Cd and Cu were found to be within the safety limit. Phytochemicals which include alkaloids, flavonoids, saponins, tannins, carbohydrates and triterpenes were detected in both aqueous and methanolic extracts. The LD50 of Burkea africana was found to be greater than 5000 mg /kg and could be considered safe for consumption. Keywords: Elemental analysis, Burkea africana, Pharmacognostic, Phytochemica

Dengue virus is hyperendemic in Nigeria from 2009 to 2020: A contemporary systematic review

Backround: Data on Dengue virus (DENV) infection prevalence, geographic distribution and risk factors are necessary to direct appropriate utilization of existing and emerging control strategies. This study aimed to determine the pooled prevalence, risk factors of DENV infection and the circulating serotypes within Nigeria from January 1, 2009 to December 31, 2020. Materials and methods: Twenty-one studies out of 2,215 available articles were eligible and included for this systematic review. Relevant articles were searched, screened and included in this study according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) criteria. The risk of bias in primary studies was assessed by Cochrane's method. Heterogeneity of pooled prevalence was calculated using the chi-square test on Cochrane's Q statistic, which was quantified by I-square values. The random-effects analyses of proportions were used to determine the pooled prevalence of DENV antibodies, antigen and RNA from eligible studies. Results: Of these, 3 studies reported co-circulation of all the 4 serotypes, while 2 separately reported co-circulation of DENV-1 &2 and DENV-1 to -3. All the antibody-based studies had significantly high heterogeneity (I2 >90%, P 0.05). The pooled prevalence of DENV IgM, IgG, RNA, NS1 and neutralizing antibodies were 16.8%, 34.7%, 7.7%, 7.7% and 0.7%, respectively. Southeast Nigeria had the highest pooled DENV-IgG seropositivity, 77.1%. Marital status, gender, educational level and occupation status, the proximity of residence to refuse dumpsite, frequent use of trousers and long sleeve shirts were significantly associated with DENV IgG seropositivity (P <0.05). Conclusion: Based on these findings, it can be inferred that Nigeria is hyperendemic for Dengue fever and needs concerted efforts to control its spread within and outside the country

Population and fertility by age and sex for 195 countries and territories, 1950–2017: a systematic analysis for the Global Burden of Disease Study 2017

Background: Population estimates underpin demographic and epidemiological research and are used to track progress on numerous international indicators of health and development. To date, internationally available estimates of population and fertility, although useful, have not been produced with transparent and replicable methods and do not use standardised estimates of mortality. We present single-calendar year and single-year of age estimates of fertility and population by sex with standardised and replicable methods. Methods: We estimated population in 195 locations by single year of age and single calendar year from 1950 to 2017 with standardised and replicable methods. We based the estimates on the demographic balancing equation, with inputs of fertility, mortality, population, and migration data. Fertility data came from 7817 location-years of vital registration data, 429 surveys reporting complete birth histories, and 977 surveys and censuses reporting summary birth histories. We estimated age-specific fertility rates (ASFRs; the annual number of livebirths to women of a specified age group per 1000 women in that age group) by use of spatiotemporal Gaussian process regression and used the ASFRs to estimate total fertility rates (TFRs; the average number of children a woman would bear if she survived through the end of the reproductive age span [age 10–54 years] and experienced at each age a particular set of ASFRs observed in the year of interest). Because of sparse data, fertility at ages 10–14 years and 50–54 years was estimated from data on fertility in women aged 15–19 years and 45–49 years, through use of linear regression. Age-specific mortality data came from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2017 estimates. Data on population came from 1257 censuses and 761 population registry location-years and were adjusted for underenumeration and age misreporting with standard demographic methods. Migration was estimated with the GBD Bayesian demographic balancing model, after incorporating information about refugee migration into the model prior. Final population estimates used the cohort-component method of population projection, with inputs of fertility, mortality, and migration data. Population uncertainty was estimated by use of out-of-sample predictive validity testing. With these data, we estimated the trends in population by age and sex and in fertility by age between 1950 and 2017 in 195 countries and territories. Findings: From 1950 to 2017, TFRs decreased by 49\ub74% (95% uncertainty interval [UI] 46\ub74–52\ub70). The TFR decreased from 4\ub77 livebirths (4\ub75–4\ub79) to 2\ub74 livebirths (2\ub72–2\ub75), and the ASFR of mothers aged 10–19 years decreased from 37 livebirths (34–40) to 22 livebirths (19–24) per 1000 women. Despite reductions in the TFR, the global population has been increasing by an average of 83\ub78 million people per year since 1985. The global population increased by 197\ub72% (193\ub73–200\ub78) since 1950, from 2\ub76 billion (2\ub75–2\ub76) to 7\ub76 billion (7\ub74–7\ub79) people in 2017; much of this increase was in the proportion of the global population in south Asia and sub-Saharan Africa. The global annual rate of population growth increased between 1950 and 1964, when it peaked at 2\ub70%; this rate then remained nearly constant until 1970 and then decreased to 1\ub71% in 2017. Population growth rates in the southeast Asia, east Asia, and Oceania GBD super-region decreased from 2\ub75% in 1963 to 0\ub77% in 2017, whereas in sub-Saharan Africa, population growth rates were almost at the highest reported levels ever in 2017, when they were at 2\ub77%. The global average age increased from 26\ub76 years in 1950 to 32\ub71 years in 2017, and the proportion of the population that is of working age (age 15–64 years) increased from 59\ub79% to 65\ub73%. At the national level, the TFR decreased in all countries and territories between 1950 and 2017; in 2017, TFRs ranged from a low of 1\ub70 livebirths (95% UI 0\ub79–1\ub72) in Cyprus to a high of 7\ub71 livebirths (6\ub78–7\ub74) in Niger. The TFR under age 25 years (TFU25; number of livebirths expected by age 25 years for a hypothetical woman who survived the age group and was exposed to current ASFRs) in 2017 ranged from 0\ub708 livebirths (0\ub707–0\ub709) in South Korea to 2\ub74 livebirths (2\ub72–2\ub76) in Niger, and the TFR over age 30 years (TFO30; number of livebirths expected for a hypothetical woman ageing from 30 to 54 years who survived the age group and was exposed to current ASFRs) ranged from a low of 0\ub73 livebirths (0\ub73–0\ub74) in Puerto Rico to a high of 3\ub71 livebirths (3\ub70–3\ub72) in Niger. TFO30 was higher than TFU25 in 145 countries and territories in 2017. 33 countries had a negative population growth rate from 2010 to 2017, most of which were located in central, eastern, and western Europe, whereas population growth rates of more than 2\ub70% were seen in 33 of 46 countries in sub-Saharan Africa. In 2017, less than 65% of the national population was of working age in 12 of 34 high-income countries, and less than 50% of the national population was of working age in Mali, Chad, and Niger. Interpretation: Population trends create demographic dividends and headwinds (ie, economic benefits and detriments) that affect national economies and determine national planning needs. Although TFRs are decreasing, the global population continues to grow as mortality declines, with diverse patterns at the national level and across age groups. To our knowledge, this is the first study to provide transparent and replicable estimates of population and fertility, which can be used to inform decision making and to monitor progress. Funding: Bill &amp; Melinda Gates Foundation

Phytochemical analyses, anxiolytic and anti-amnesic effect of methanol stem bark extract of Vitex doniana (sweet) in mice

This study investigated the oral acute toxicity (LD50), anxiolytic and anti-amnesic effects of the methanol stem bark extract of Vitex doniana (MSVD) in mice. The anxiolytic and anti-amnesic effects were assessed using standard protocols. The phytoconstituents in MSVD was quantified. The MSVD was further analysed using Ultraviolet-visible (UV-VIS) and Fourier Transform Infra red (FTIR) fingerprints. The LD50 was &gt; 5000 mg/kg suggesting its safety. The MSVD (125, 250 and 500 mg/kg, p.o.) showed significant (p&lt;0.05) increase in the percentage open arm entry and duration on elevated plus maze as well as reduced the anxiety index as indexed from the open arm avoidance index indicating an anti-anxiety effect. Subsequently, MSVD significantly (p&lt;0.05) increased the percentage alternation reduced by scopolamine consistent with anti-amnesic effect. The phytoconstituents estimation of MSVD showed abundance of flavonoids. The UV-VIS spectra corresponded to the presence of flavonoid, phenolic acid derivatives and terpenoids while the FTIR revealed the presence of amine, hydroxyl, alkane, carboxylic, amide, ether, aromatic and carbonyl functional groups among others in MSVD. This study therefore, concluded that flavonoids, either in synergy or additive with other phytocompounds in MSVB may be responsible for the observed anxiolytic and anti-amnesic effects elicited by MSVD in this study

Isolation and characterization of Streptococcus pyogenes from sputum and throat of patients with respiratory tract infections in Zaria, Nigeria

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An Unusual Case of Severe Varicella Zoster Infection Complicated by Acute Renal Failure in an 8-year-old Nigerian Boy

Background: Varicella zoster virus (VZV) infection is highly contagious and has a worldwide distribution. It is predominantly a childhood disease and frequently runs a benign course. However, serious complications such as target organ damage could occur in older immunocompetent children. Acute kidney injury (AKI) arising from varicella infection is one of the rare complications in children. Case Report: The patient presented with body rash typical of varicella, fever, cough, difficulty in breathing, vomiting and dark urine. He had contact with his two siblings with similar rashes. He was febrile, centrally cyanosed with generalised lymphadenopathy and body rashes at different stages of development. He had tachypnoea, dyspnoea and widespread coarse crepitations. Kidneys were enlarged and urinalysis revealed microscopic haematuria of 4+ and proteinuria 1+. Initial assessment of chickenpox with pneumonia and nephritis was made. He received oral acyclovir among other interventions. His estimated glomerular filtration rate dropped from 120 to 8.71mls/min/1.73m2. He had 3 sessions of haemodialysis, his biochemical parameters normalised and has been on follow-up for one year with normal renal function. Conclusion: Acute varicella infection can lead to clinically significant kidney failure in otherwise healthy children. Prompt renal replacement therapy could result in complete recovery of kidney function