Optical pulsations from a transitional millisecond pulsar

Weakly magnetic, millisecond spinning neutron stars attain their very fast rotation through a 1E8-1E9 yr long phase during which they undergo disk-accretion of matter from a low mass companion star. They can be detected as accretion-powered millisecond X-ray pulsars if towards the end of this phase their magnetic field is still strong enough to channel the accreting matter towards the magnetic poles. When mass transfer is much reduced or ceases altogether, pulsed emission generated by particle acceleration in the magnetosphere and powered by the rotation of the neutron star is observed, preferentially in the radio and gamma-ray bands. A few transitional millisecond pulsars that swing between an accretion-powered X-ray pulsar regime and a rotationally-powered radio pulsar regime in response to variations of the mass in-flow rate have been recently identified. Here we report the detection of optical pulsations from a transitional pulsar, the first ever from a millisecond spinning neutron star. The pulsations were observed when the pulsar was surrounded by an accretion disk and originated inside the magnetosphere or within a few hundreds of kilometres from it. Energy arguments rule out reprocessing of accretion-powered X-ray emission and argue against a process related to accretion onto the pulsar polar caps; synchrotron emission of electrons in a rotation-powered pulsar magnetosphere seems more likely.Comment: 32 pages, 7 figures. The first two authors contributed equally to this wor

Precision Measurement of KS Meson Lifetime with the KLOE detector

Using a large sample of pure, slow, short lived K0 mesons collected with KLOE detector at DaFne, we have measured the KS lifetime. From a fit to the proper time distribution we find tau = (89.562 +- 0.029_stat +- 0.043_syst) ps. This is the most precise measurement today in good agreement with the world average derived from previous measurements. We observe no dependence of the lifetime on the direction of the Ks.Comment: 5 pages, 7 figure

Direct CP violation in two-body hadronic charmed meson decays

Motivated by the recent observation of CP violation in the charm sector by LHCb, we study direct CP asymmetries in the standard model (SM) for the singly Cabibbo-suppressed two-body hadronic decays of charmed mesons using the topological-diagram approach. In this approach, the magnitude and the phase of topological weak annihilation amplitudes which arise mainly from final-state rescattering can be extracted from the data. Consequently, direct CP asymmetry $a_{dir}^{\rm (tree)}$ at tree level can be reliably estimated. In general, it lies in the range $10^{-4}<a_{dir}^{\rm (tree)}<10^{-3}$. Short-distance QCD penguins and penguin annihilation are calculated using QCD factorization. Their effects are generally small, especially for $D\to VP$ modes. Since weak penguin annihilation receives long-distance contributions from the color-allowed tree amplitude followed by final-state rescattering, it is expected to give the dominant contribution to the direct CP violation in the decays $D^0\to K^+K^-$ and $D^0\to \pi^+\pi^-$ in which $a_{dir}^{\rm (tree)}$ is absent. The maximal $\Delta a_{CP}^{\rm dir}$, the direct CP asymmetry difference between the above-mentioned two modes, allowed in the SM is around -0.25%, more than $2\sigma$ away from the current world average of $-(0.645\pm 0.180)$.Comment: 19 pages, 2 figures; typos correcte

Unexpected death of a ventilator-dependent amyotrophic lateral sclerosis patient

Background: Amyotrophic lateral sclerosis (ALS) is a fatal, progressive, neurodegenerative disease and most patients affected die of respiratory compromise and/or pneumonia within 2â3 years of diagnosis. As ALS progresses, ventilator assistance is required. In the end stages of the disease, patients suffer from respiratory failure and may become ventilator-dependent. Deaths due to malfunction of mechanical ventilators are reported but there are very few forensic autopsy records. We report the case of a 69-year-old ALS female ventilator-dependent, trachostomised patient who was found dead by her husband, with the ventilator in âstand-byâ mode. Method: A forensic autopsy was performed. Samples of internal organs were taken for histological and toxicological examination. The ventilator internal memory was also analysed and tested in order to find possible malfunction. Results: Gross examination did not reveal any sign of trauma but showed brain and lung congestion. Pulmonary histological examination revealed thickening of peribronchial interstitial space, alveolar over-distension, break of inter-alveolar walls and diffuse alveolar haemorrhages. Focal microhemorrhages were also detected in other organs. Analysis of the ventilator internal memory showed that during the night of death, there had been several voltage drops. Specific tests revealed malfunction of the internal battery which was unable to provide the necessary voltage, as a consequence the ventilator switched off, stopping ventilation. Battery malfunction reduced the volume of the ventilator alarm, which was not heard by the caregiver. Conclusion: Histological pattern, with acute pulmonary emphysema and focal polivisceral haemorrhages, is strongly suggestive of a death due to âacuteâ asphyxia. The authors also discuss the need for strict supervision and follow up of these ventilatory dependent patients and their devices. Resumo: Introdução: A Esclerose Lateral Amiotrófica (ELA) é uma doença fatal, progressiva e neurodegenerativa e a maioria dos doentes afectados morrerão de falha respiratória e/ou pneumonia 2 ou 3 anos após o diagnóstico. à medida que a ELA progride torna-se necessária a assistência ventilatória. Nos estágios finais da doença, os doentes sofrem de insuficiência respiratória e podem tornar-se dependentes do ventilador. São conhecidas mortes devido ao mau funcionamento de ventiladores mecânicos mas existem poucos registos forenses de tal situação. Relatamos o caso de uma doente de 69 anos com ELA, traqueostomizada e dependente do ventilador, que foi encontrada morta pelo seu marido com o ventilador em modo de espera («stand-by»). Método: Foi realizada uma autópsia forense. Foram recolhidas amostras dos órgãos internos para exame toxicológico e histológico. A memória interna do ventilador foi também analisada e testada de modo a descobrir uma possível avaria. Resultados: O exame macroscópico não revelou qualquer sinal de trauma mas indicou congestão cerebral e pulmonar. O exame pulmonar histológico revelou o espessamento do espaço intersticial peribrônquico, sobredistensão alveolar, quebra de paredes interalveolares e hemorragias alveolares difusas. Foram detetadas micro-hemorragias focais noutros órgãos. A análise da memória interna do ventilador mostrou que, durante a noite da morte, houve diversas quedas de tensão. Testes específicos revelaram o mau funcionamento da bateria interna que não conseguiu fornecer a tensão necessária, consequentemente o ventilador desligou-se, parando a ventilação. O mau funcionamento da bateria reduziu o volume do alarme do ventilador, que não foi ouvido pelo prestador de cuidados. Conclusão: O padrão histológico, com enfisema pulmonar agudo e hemorragias focais poliviscerais, é fortemente indicador de morte devido a asfixia «aguda». Os autores discutem também a necessidade de uma supervisão rigorosa e seguimento destes doentes dependentes do ventilador e dos seus equipamentos. Keywords: Amyotrophic lateral sclerosis, Acute respiratory failure, Home mechanical ventilation, Palavras-chave: Esclerose lateral amiotrófica, Insuficiência respiratória aguda, Ventilação mecânica doméstic

The effect of estrogen and tamoxifen on hepatocyte proliferation in Vivo and in Vitro

We have previously shown that changes in estrogen‐hepatocyte interaction occur during liver regeneration. Following 70% hepatectomy, estrogen levels in the blood were elevated, the number of estrogen receptors in the liver was increased and there was an active translocation of estrogen receptors from the cytosol to the nucleus. The injection of tamoxifen, an estrogen antagonist, inhibits hepatocyte proliferation following partial hepatectomy. The administration of 1 μg tamoxifen per gm body weight at zero time or 6 hr after the operation resulted in a significant inhibition both of DNA synthesis and of the number of cells in mitosis. Injections of tamoxifen 12 hr or later after the operation had no effect. Concomitant injections of equimolar amounts of estrogen abolished the inhibition by tamoxifen. The effects of estrogen and tamoxifen were also tested on hepatocytes in primary culture. Estrogens in the presence of 5% normal rat serum stimulated hepatocyte DNA synthesis as determined by [3H]thymidine incorporation and the labeling index, whereas epidermal growth factor‐induced DNA synthesis in the absence of normal rat serum was strongly inhibited. Tamoxifen, in contrast, inhibited DNA synthesis of hepatocytes in the presence of 5% normal rat serum and reversed the stimulatory effect of estrogen in the same system. Attempts to elucidate the mechanism of tamoxifen inhibition in vitro indicated that one effect of tamoxifen is to prevent the amiloride‐sensitive Na+ influx necessary to initiate hepatocyte proliferation. Copyright © 1989 American Association for the Study of Liver Disease

A combinatorial approach to gene expression analysis: DNA microarrays.

The microarray technology is based on analytical tools that parallelize the quantitative and qualitative analysis of nucleic acids, proteins and tissue sections one of its more recent evolutions-. By miniaturizing the size of the reaction and sensing area, microarrays allow to assess at the activity of thousands of genes in a given tissue or cell line at once in a rapid and quantitative way, and to carry out serial comparative tests in multiple samples. These tools, that stem from the innovations resulting from the technological improvements and knowledge arising from the genome sequencing projects, can be considered as a combinatorial technique that can rapidly provide significant information about complex cellular pathways and processes within one or few ‘‘mass scale’’ and comprehensive testing of a biological sample’s composition

Developement of real time diagnostics and feedback algorithms for JET in view of the next step

Real time control of many plasma parameters will be an essential aspect in the development of reliable high performance operation of Next Step Tokamaks. The main prerequisites for any feedback scheme are the precise real-time determination of the quantities to be controlled, requiring top quality and highly reliable diagnostics, and the availability of robust control algorithms. A new set of real time diagnostics was recently implemented on JET to prove the feasibility of determining, with high accuracy and time resolution, the most important plasma quantities. With regard to feedback algorithms, new model&#8211;based controllers were developed to allow a more robust control of several plasma parameters. Both diagnostics and algorithms were successfully used in several experiments, ranging from H-mode plasmas to configuration with ITBs. Since elaboration of computationally heavy measurements is often required, significant attention was devoted to non-algorithmic methods like Digital or Cellular Neural/Nonlinear Networks. The real time hardware and software adopted architectures are also described with particular attention to their relevance to ITER.Comment: 12th International Congress on Plasma Physics, 25-29 October 2004, Nice (France

A new cylindrical borehole detector for radiographic imaging with muons

Muon radiography is a methodology which enables measuring the mass distribution within large objects. It exploits the abundant flux of cosmic muons and uses detectors with different technologies depending on the application. As the sensitive surface and geometric acceptance are two fundamental parameters for increasing the collection of muons, the optimization of the detectors is very significant. Here we show a potentially innovative detector of size and shape suitable to be inserted inside a borehole, that optimizes the sensitive area and maximizes the angular acceptance thanks to its cylindrical geometry obtained using plastic arc-shaped scintillators. Good spatial resolution is obtained with a reasonable number of channels. The dimensions of the detector make it ideal for use in 25 cm diameter wells. Detailed simulations based on Monte Carlo methods show great cavity detection capability. The detector has been tested in the laboratory, achieving overall excellent performance