Biotechnology a Source of Knowledge in Agreement with Green Strategies for the Conservation of Cultural Assets

In this study, conservative strategies are applied in order to limit any irreversible damage and to characterize and quantify microbial colonization that may induce the deterioration of constitutive heritage materials; subsequently, suitable antimicrobial interventions are performed based on the use of biotechnology. This paper presents a summary of the basic and applied biotechnology research developed in the Laboratory of Biology and Biotechnology for Cultural Heritage (LaBBCH, University of Palermo, Italy). It shows that biotechnology has an evidently important role in the preservation and restoration of cultural assets, also taking into consideration that “Prevention is better than cure”, a milestone in the field of human health that can be shifted and applied to the “cure and preservation” of cultural assets. Moreover, biotechnological tools offer great potential for application, thanks to the increasing interaction between the worlds of art and science, thus opening the way for advanced innovation in the conservation and restoration field of art works

Cold-active molecules for a sustainable preservation and restoration of historic-artistic manufacts

In the last decades biotechnology research provides sustainable alternatives to traditional procedures for preventive preservation of cultural assets. Recently, bioactive molecules (BMs) isolated from marine invertebrate organisms have been isolated and tested for bioremoval of protein layers (BMP) or to controlling microbial colonization (BMA), acting at temperature lower than 30°C. The Protease or Antimicrobial activity was tested on ad hoc assembled specimens and on different historic-artistic manufacts. In bio-removing protocol BMP molecules were applied as gelled solutions, in order to guarantees a selective action, respectful of constitutive materials and manufact integrity. Peculiarity of Protease bioactive molecules is the temperature of action, lower than 30°C. Instead, BMAs molecules have been tested to control bacteria and fungi colonization in laboratory specimens. In our hypothesis these novel molecules provide an important contribution to the development of innovative protocols safe both for the environment and conservator health, representing a valid alternative to traditional methods according to the preventive conservation and "Minimal Intervention" concept in restoration procedures

Saggi sull’attività antimicrobica di molecole bioattive in interventi di foderatura di dipinti

In questo studio è stato valutato l’utilizzo di molecole antimicrobiche estratte da organismi marini invertebrati (Anthozoa), al fine di limitare o inibire la crescita microbica su materiali impiegati per il restauro di manufatti storico-artistici. I ceppi batterici e fungini utilizzati nei saggi antimicrobici, sono stati isolati da porzioni di tele, stratificate con colla, impiegate nella foderatura di dipinti. Mediante un approccio integrato che comprende tecniche di microscopia e tecniche molecolari, sono state identificate colonie batteriche appartenenti ai generi Enterobacter e Micrococcus e fungine appartenenti ai generi Aspergillus e Penicillium. L’attività antimicrobica delle molecole (BMA1, BMA2, BMA3) è stata testata sui ceppi microbici identificati, definendo le corrispondenti Concentrazioni Minime Inibenti (MIC) e le Concentrazioni Minime Battericide/Fungicide (MBC/MFC). Inoltre, l’attività antimicrobica è stata testata su provini assemblati ad hoc in laboratorio, realizzati con colla pasta stratificata su due tipi di tela (lino, sintetica), simulando un intervento di foderatura. La presenza delle molecole BMA nella colla pasta ha contrastato la crescita microbica, inibendola completamente per il campione BMA1 e parzialmente in presenza di BMA2 e BMA3. L’utilizzo di queste molecole nel campo della conservazione dei beni culturali, costituisce un importante contributo allo sviluppo di tecnologie innovative, nel rispetto delle procedure del restauro conservativo

Cross-Country Adaptation of a Psychological Flexibility Measure: The Comprehensive Assessment of Acceptance and Commitment Therapy Processes

Cultural adaptation; Linguistic validation; Psychological flexibilityAdaptación cultural; Validación lingüística; Flexibilidad psicológicaAdaptació cultural; Validació lingüística; Flexibilitat psicològicaPurpose: The Comprehensive assessment of Acceptance and Commitment Therapy (ACT) processes (CompACT) is a 23-item self-report questionnaire assessing psychological flexibility, which is the overarching construct underpinning the ACT framework. We conducted a two-phase project to develop validated versions of the CompACT in three languages: phase 1—cross-cultural adaptation; and phase 2—psychometric validation of the questionnaire for use in Italy, Germany and Spain. This article focuses on the first phase. Methods: We translated and culturally adapted the CompACT in the three target languages, following the ISPOR TCA Task Force guidelines. The process was overseen by a translation panel (three translators, at least two multiple sclerosis (MS) researchers and a lay person), ACT experts and clinicians from the research team of each country and the original CompACT developers. We debriefed the new questionnaire versions via face-to-face interviews with a minimum of four adults from the general population (GP) and four adults with MS in each country. Results: The translation-adaptation process went smoothly in the three countries, with some items (7 in Italy, 4 in Germany, 6 in Spain) revised after feedback from ACT experts. Cognitive debriefing showed that the CompACT was deemed easy to understand and score in each target country by both GP and MS adults. Conclusions: The Italian, German and Spanish versions of the CompACT have semantic, conceptual and normative equivalence to the original scale and good content validity. Our findings are informative for researchers adapting the CompACT and other self-reported outcome measures into multiple languages and cultures.This study is supported by the “REHABILITATION IN MULTIPLE SCLEROSIS-RIMS European network for best practice and resource” (RIMS GRANT PROGRAM 2018 to AMG). The funding source had no role in study design, data collection, data analysis, data interpretation or report writing

MODERN TECHNOLOGIES FOR CONSERVATION OF VILLA ZITO PAINTINGS COLLECTION (PALERMO – SICILY)

In this study we describe the conservative integrated approach performed on 40 paintings, dating from different period (XVII- XX century), belonging to “Fondazione Sicilia “ and exposed in Villa Zito paint gallery in Palermo (Mazzocca 2015). Through a multidisciplinary approach both constitutive materials and state of conservation have been determined. Particularly, pigments and executive techniques were identified by non-invasive diagnostic investigation (XRF analysis, UV fluorescence acquisition, and IR Reflectography) as well as previous restorations events. The study has included the revealing of microbial colonization by using non-invasive sampling methods (Nylon membrane fragment) and molecular biology tools for the identification of microbial taxa (Palla et al 2010). For this reason another aim is the application of sustainable methods as alternative to traditional restoration procedures, which can sometimes be detrimental for artworks, humans and environment. In particular, in vitro culture (Nutrient, Sabouraud agar), optical microscopy observations (O.M.) and molecular biology investigations (genomic DNA extraction, PCR, sequencing and sequence analysis) reveal the presence of bacteria colonies belonging to Micrococcus sp., Staphylococcus sp. and Bacillus sp. In order to remove undesired layers appropriate cleaning protocols have been tested, using gelled solutions at specific pH values or dry cleaning methods (Cremonesi 2011; Daudin-Schotte et al 2013). Agar-Agar or Gellan-Gum hard gels have been utilized in order to remove dirty or proteinaceous layers by adding specific enzymatic solutions in the gels. The enzymatic cleaning has been performed at room temperature (20-23°C); the removal (total/partial) has been obtained after 10 minutes of application (Barresi et al 2015). Instead, on unvarnished and water sensitive paintings, the RCE-dry cleaning method (Rijksdienst voor het Cultureel Erfgoed- Netherland Institute for Cultural Heritage) have been applied following ad hoc protocols (Giordano et al 2014; Daudin-Schotte et al 2013)

Viability of a MSQOL-54 general health-related quality of life score using bifactor model

Background MSQOL-54 is a multidimensional, widely-used, health-related quality of life (HRQOL) instrument specific for multiple sclerosis (MS). Findings from the validation study suggested that the two MSQOL-54 composite scores are correlated. Given this correlation, it could be assumed that a unique total score of HRQOL may be calculated, with the advantage to provide key stakeholders with a single overall HRQOL score. We aimed to assess how well the bifactor model could account for the MSQOL-54 structure, in order to verify whether a total HRQOL score can be calculated. Methods A large international database (3669 MS patients) was used. By means of confirmatory factor analysis, we estimated a bifactor model in which every item loads onto both a general factor and a group factor. Fit of the bifactor model was compared to that of single and two second-order factor models by means of Akaike information and Bayesian information criteria reduction. Reliability of the total and subscale scores was evaluated with Mc Donald's coefficients (omega, and omega hierarchical). Results The bifactor model outperformed the two second-order factor models in all the statistics. All items loaded satisfactorily (>= 0.40) on the general HRQOL factor, except the sexual function items. Omega coefficients for total score were very satisfactory (0.98 and 0.87). Omega hierarchical for subscales ranged between 0.22 to 0.57, except for the sexual function (0.70). Conclusions The bifactor model is particularly useful when it is intended to acknowledge multidimensionality and at the same time take account of a single general construct, as the HRQOL related to MS. The total raw score can be used as an estimate of the general HRQOL latent score

Applying multidimensional computerized adaptive testing to the MSQOL-54: a simulation study

Background: The Multiple Sclerosis Quality of Life-54 (MSQOL-54) is one of the most commonly-used MS-specific health-related quality of life (HRQOL) measures. It is a multidimensional, MS-specific HRQOL inventory, which includes the generic SF-36 core items, supplemented with 18 MS-targeted items. Availability of an adaptive short version providing immediate item scoring may improve instrument usability and validity. However, multidimensional computerized adaptive testing (MCAT) has not been previously applied to MSQOL-54 items. We thus aimed to apply MCAT to the MSQOL-54 and assess its performance. Methods: Responses from a large international sample of 3669 MS patients were assessed. We calibrated 52 (of the 54) items using bifactor graded response model (10 group factors and one general HRQOL factor). Then, eight simulations were run with different termination criteria: standard errors (SE) for the general factor and group factors set to different values, and change in factor estimates from one item to the next set at < 0.01 for both the general and the group factors. Performance of the MCAT was assessed by the number of administered items, root mean square difference (RMSD), and correlation. Results: Eight items were removed due to local dependency. The simulation with SE set to 0.32 (general factor), and no SE thresholds (group factors) provided satisfactory performance: the median number of administered items was 24, RMSD was 0.32, and correlation was 0.94. Conclusions: Compared to the full-length MSQOL-54, the simulated MCAT required fewer items without losing precision for the general HRQOL factor. Further work is needed to add/integrate/revise MSQOL-54 items in order to make the calibration and MCAT performance efficient also on group factors, so that the MCAT version may be used in clinical practice and research

Conversion to secondary progressive multiple sclerosis: patient awareness and needs. results from an online survey in Italy and Germany

Background: Few studies have investigated the experiences of patients around the conversion to secondary progressive multiple sclerosis (SPMS). ManTra is a mixed-method, co-production research project conducted in Italy and Germany to develop an intervention for newly-diagnosed SPMS patients. In previous project actions, we identified the needs and experiences of patients converting to SPMS via literature review and qualitative research which involved key stakeholders.Aims: The online patient survey aimed to assess, on a larger and independent sample of recently-diagnosed SPMS patients: (a) the characteristics associated to patient awareness of SPMS conversion; (b) the experience of conversion; (c) importance and prioritization of the needs previously identified.Methods: Participants were consenting adults with SPMS since <= 5 years. The survey consisted of three sections: on general and clinical characteristics; on experience of SPMS diagnosis disclosure (aware participants only); and on importance and prioritization of 33 pre-specified needs.Results: Of 215 participants, those aware of their SPMS diagnosis were 57% in Italy vs. 77% in Germany (p = 0.004). In both countries, over 80% of aware participants received a SPMS diagnosis from the neurologist; satisfaction with SPMS disclosure was moderate to high. Nevertheless, 28-35% obtained second opinions, and 48-56% reported they did not receive any information on SPMS. Participants actively seeking further information were 63% in Germany vs. 31% in Italy (p < 0.001).Variables independently associated to patient awareness were geographic area (odds ratio, OR 0.32, 95% CI 0.13-0.78 for Central Italy; OR 0.21, 95% CI 0.08-0.58 for Southern Italy [vs. Germany]) and activity limitations (OR 7.80, 95% CI 1.47-41.37 for dependent vs. autonomous patients).All pre-specified needs were scored a lot or extremely important, and two prioritized needs were shared by Italian and German patients: "physiotherapy" and "active patient care involvement." The other two differed across countries: "an individualized health care plan" and "information on social rights and policies" in Italy, and "psychological support" and "cognitive rehabilitation" in Germany.Conclusions: Around 40% of SPMS patients were not aware of their disease form indicating a need to improve patient-physician communication. Physiotherapy and active patient care involvement were prioritized in both countries

How future surgery will benefit from SARS-COV-2-related measures: a SPIGC survey conveying the perspective of Italian surgeons

COVID-19 negatively affected surgical activity, but the potential benefits resulting from adopted measures remain unclear. The aim of this study was to evaluate the change in surgical activity and potential benefit from COVID-19 measures in perspective of Italian surgeons on behalf of SPIGC. A nationwide online survey on surgical practice before, during, and after COVID-19 pandemic was conducted in March-April 2022 (NCT:05323851). Effects of COVID-19 hospital-related measures on surgical patients' management and personal professional development across surgical specialties were explored. Data on demographics, pre-operative/peri-operative/post-operative management, and professional development were collected. Outcomes were matched with the corresponding volume. Four hundred and seventy-three respondents were included in final analysis across 14 surgical specialties. Since SARS-CoV-2 pandemic, application of telematic consultations (4.1% vs. 21.6%; p < 0.0001) and diagnostic evaluations (16.4% vs. 42.2%; p < 0.0001) increased. Elective surgical activities significantly reduced and surgeons opted more frequently for conservative management with a possible indication for elective (26.3% vs. 35.7%; p < 0.0001) or urgent (20.4% vs. 38.5%; p < 0.0001) surgery. All new COVID-related measures are perceived to be maintained in the future. Surgeons' personal education online increased from 12.6% (pre-COVID) to 86.6% (post-COVID; p < 0.0001). Online educational activities are considered a beneficial effect from COVID pandemic (56.4%). COVID-19 had a great impact on surgical specialties, with significant reduction of operation volume. However, some forced changes turned out to be benefits. Isolation measures pushed the use of telemedicine and telemetric devices for outpatient practice and favored communication for educational purposes and surgeon-patient/family communication. From the Italian surgeons' perspective, COVID-related measures will continue to influence future surgical clinical practice

Il metodo Dry Cleaning per la pulitura delle opere d’arte: il caso studio delle mensole lignee policrome del castello di Trabia

Le superfici policrome non verniciate pongono delle specifiche pro - blematiche di conservazione, legate soprattutto alla spiccata sensibilità. Agli agenti esterni (temperatura, pul - viscolo atmosferico, umidità, etc.). Anche le operazioni di pulitura di tali opere si scontrano con delle problematiche particolari, legate a difficoltà di intervento utilizzando le metodiche tradizionali1. A tali quesiti è da aggiungere, per di più, che le pellicole pittoriche realizzate senza uno strato protettivo necessitano, con maggiore frequenza, di interventi conservativi