Occurrence of fungi in bean seeds ‘Red Mexican’ and its effect on the germination

The seed-born fungi can be transmitted to long distances, also to reduce the viability of seeds. The objective of this study was to verify the occurring pathogens on common bean ‘Red mexican’ seed and evaluate its effect in the obtaining normal seedlings. Therefore, bean seeds 'Red mexican' were submitted to blotter test (25 seeds Gerbox-1) in four different dates. The design was completely randomized design (CRD) and each assessed data had eight replicates (Gerbox). The fungi incidence and obtaining normal seedlings were evaluated at 5 and 9 days, respectively. It was verified a larger incidence for Aspergillus sp. (19 to 57%) when compared to Cladosporium cladosporioides (11 to 29%). The incidence of the genus Aspergillus sp. and C. cladosporioides did not interfere in the percentage of normal seedlings, due to the escape mechanism of the seedlings to pathogens during the germination process.The seed-born fungi can be transmitted to long distances, for the transport of the same, in addition to harming seed viability. Therefore, the objective of this study was to verify the occurring fungi on common bean ‘Red mexican’ seed and evaluate its effect in the development of normal seedlings. Therefore, stored bean seeds ‘Red mexican’ were submitted to blotter test in four different dates. The design was completely randomized (CRD) and each assessed data had eight replicates. Each experimental unit (repetition) consisted in a Gerbox containing 25 seeds. The fungi incidence and development of normal seedlings were evaluated at 5 and 7 days, respectively. It was verified a larger incidence for Aspergillus sp. (19% to 57%) when compared to Cladosporium cladosporioides (11% to 29%). The incidence of the fungi Aspergillus sp. and C. cladosporioides did not interfere in the percentage of normal seedlings, due to the escape mechanism of the seedlings to pathogens during the germination process

Prevalência de escoliose em escolares na cidade de Fortaleza, Ceará, Brasil

A escoliose é uma das deformidades que afetam a coluna vertebral que surge durante a fase de aceleração do crescimento vertebral, por essa razão, as crianças são os alvos mais vulneráveis desta enfermidade. O objetivo principal desse estudo foi verificar a prevalência da escoliose em escolares na cidade de Fortaleza, Ceará. Trata-se de uma pesquisa de caráter descritivo, transversal, de campo, exploratória com abordagem quantitativa. As informações foram coletadas por meio de um questionário com perguntas sobre dados pessoais e características ambientais. A avaliação postural foi realizada pelo teste de Adams, em uma amostra de 46 escolares matriculados no 5º ano e 6º ano do ensino fundamental numa escola da rede privada na cidade de Fortaleza, Ceará. Com relação ao gênero dos alunos que participaram da amostra, 60,87% (n=28) são do sexo feminino e 39,14% (n=18) do sexo masculino e, desses, 67,39% (n=31) estudantes do 5º ano e 6º ano praticavam atividades físicas. O teste de Adams positivo foi detectado em 71,73% (n=33) da amostra estudada. Concluímos que a má postura em sala de aula e ao dormir favorecem o surgimento da escoliose, pois existiu uma alta prevalência dessa alteração postural nos estudantes analisados

MOBILIZAÇÃO ARTICULAR DE MAITLAND COMO TRATAMENTO DA CERVICALGIA CRÔNICA: REVISÃO NARRATIVA DA LITERATURA

Introduction: Chronic neck pain is a common multifactorial musculoskeletal disorder that can cause disability. The limitations generated by neck pain compromise the individual's biopsychosocial well-being. Among the therapeutic approaches, joint mobilization stands out through the Maitland technique. Objective: To verify the effects of the Maitland technique as a treatment of chronic neck pain. Methodology: This is a narrative review of the literature. The data were taken from studies published in English and Portuguese in the period from 2013 to 2023, in the Pubmed, Pedro, Lilacs, Scielo databases, using the following descriptors in Portuguese: neck pain, neck pain, manual therapy and Maitland, as well as in English: neck pain, neck ache, manual therapy and Maitland. A total of 990 articles were pre-selected and after applying the eligibility criteria, 5 were included. Results and Discussion: The Maitland technique alone or associated with other therapies promotes pain reduction, gains in ROM and improvements in neck disability. Conclusion: Maitland is effective in treating chronic neck pain in adults, promoting symptom improvement.Introducción: El dolor crónico de cuello es un trastorno musculoesquelético multifactorial común que puede causar incapacidad. Las limitaciones que genera el dolor de cuello comprometen el bienestar biopsicosocial del individuo. Entre los abordajes terapéuticos destaca la movilización articular de Maitland. Objetivo: Verificar los efectos de la técnica Maitland como tratamiento para dolor crónico de cuello. Metodología: Esta es una revisión narrativa de la literatura. Los datos fueron tomados de estudios publicados en inglés y portugués en el período de 2013 a 2023, en las bases de datos Pubmed, Pedro, Lilacs, Scielo, utilizando los siguientes descriptores en portugués: dolor de cuello, dolor de cuello, terapia manual y Maitland, además como en inglés: dolor de cuello, dolor de cuello, terapia manual y Maitland. Se preseleccionaron un total de 990 artículos y tras aplicar los criterios de elegibilidad se incluyeron 5. Resultados y Discusión: La técnica de Maitland sola o asociada con otras terapias promueve la reducción del dolor, ganancias en el ROM y mejoras en la discapacidad del cuello. Conclusión: Maitland es eficaz en el tratamiento del dolor crónico de cuello en adultos, promoviendo la mejora de los sintomas.Introdução: A cervicalgia crônica é um distúrbio musculoesquelético multifatorial comum que pode gerar incapacidade. As limitações geradas pela dor no pescoço comprometem o bem-estar biopsicossocial do indivíduo. Dentre as condutas terapêuticas destaca-se a mobilização articular por meio da técnica de Maitland. Objetivo: Verificar os efeitos da técnica de Maitland como tratamento da cervicalgia crônica. Metodologia: Trata-se de uma revisão narrativa da literatura.  Os dados foram retirados de estudos publicados nos idiomas inglês e português no período de 2013 a 2023, nas bases de dados Pubmed, Pedro, Lilacs, Scielo, por meio dos seguintes descritores em português: cervicalgia, dor no pescoço, terapia manual e Maitland, bem como em inglês: neck pain, neck ache, manual therapy e Maitland. Um total de 990 artigos foram pré selecionados e após a aplicação dos critérios de elegibilidade, 5 foram incluídos. Resultados e Discussão: A técnica de Maitland isolada ou associada a outras terapêuticas promove diminuição da dor, ganho de ADM e melhora da incapacidade do pescoço. Conclusão: Maitland é eficaz no tratamento da cervicalgia crônica em adultos, promovendo melhora dos sintomas

The Barretos Cancer Hospital Animal Facility: implementation and results of a dedicated platform for preclinical oncology models

The Barretos Cancer Hospital Animal Facility (BCHAF) is a unique facility in Brazil exclusively dedicated to working with animal models for cancer research. In this article, we briefly present our modern facility and the main experiments performed, focusing on mutant strains of mice (PTCH-knockout and ApcMin mice), xenograft models, and patient-derived xenografts (PDXs). Our results show the progress and challenges in establishing these models and the need for having an appropriate representation of our cancer population to better understand tumor biology and to identify cancer biomarkers, which could be putatively targeted, allowing for personalized therapy.This study was funded by the Public Ministry of Labor Campinas (Research, Prevention and Education of Occupational Cancer) and by Pio XII Foundation, Barretos Cancer Hospital internal funds, Grant Number: 13/2021

Justiça socioambiental: os progressos e as incitações entre o crescimento nas relações econômicas e o desenvolvimento social a partir da perspectiva da neutralidade tributária no RE 773992 - BA / Social and environmental justice: progress and encourages between growth in economic relations and social development from the perspective of tax neutrality in RE 773992 - BA

O presente artigo objetiva analisar a justiça socioambiental frente os progressos e as incitações entre o crescimento econômico e o desenvolvimento social a partir da perspectiva da neutralidade tributária do Supremo Tribunal Federal – STF no julgado RE 773992/BA. Tiveram como objetivos específicos investigar de acordo com a decisão do STF no RE 773992/BA as perspectivas de um novo direito fundamental garantindo a construção de um mundo sustentável trazendo uma ordem social, analisar as possibilidades de existência de uma tese coerente firmada pelo STF no julgado RE 773992/BA, que abordem a capacidade contributiva à luz do princípio do poluidor-pagador e avaliar através da decisão paradigmática do STF no RE 773992/BA a conduta utilizada no tocante a capacidade contributiva, livre concorrência e os progressos e incitações em defesa da justiça socioambiental através da extrafiscalidade. Assim, torna relevante discutir a perspectiva da neutralidade tributária no Brasil de acordo com o posicionamento do STF no julgado emblemático. A metodologia utilizada: descritivo-analítico. Quanto ao tipo: bibliográfica, incluindo artigos científicos, teses e documentos em sítios eletrônicos oficiais. Quanto à natureza: qualitativa, por tratar de verificar a questão do comportamento humano. Quanto aos fins, descrever, analisar, classificar e interpretar os fatos relativos ao problema apresentado

Energy Estimation of Cosmic Rays with the Engineering Radio Array of the Pierre Auger Observatory

The Auger Engineering Radio Array (AERA) is part of the Pierre Auger Observatory and is used to detect the radio emission of cosmic-ray air showers. These observations are compared to the data of the surface detector stations of the Observatory, which provide well-calibrated information on the cosmic-ray energies and arrival directions. The response of the radio stations in the 30 to 80 MHz regime has been thoroughly calibrated to enable the reconstruction of the incoming electric field. For the latter, the energy deposit per area is determined from the radio pulses at each observer position and is interpolated using a two-dimensional function that takes into account signal asymmetries due to interference between the geomagnetic and charge-excess emission components. The spatial integral over the signal distribution gives a direct measurement of the energy transferred from the primary cosmic ray into radio emission in the AERA frequency range. We measure 15.8 MeV of radiation energy for a 1 EeV air shower arriving perpendicularly to the geomagnetic field. This radiation energy -- corrected for geometrical effects -- is used as a cosmic-ray energy estimator. Performing an absolute energy calibration against the surface-detector information, we observe that this radio-energy estimator scales quadratically with the cosmic-ray energy as expected for coherent emission. We find an energy resolution of the radio reconstruction of 22% for the data set and 17% for a high-quality subset containing only events with at least five radio stations with signal.Comment: Replaced with published version. Added journal reference and DO

Measurement of the Radiation Energy in the Radio Signal of Extensive Air Showers as a Universal Estimator of Cosmic-Ray Energy

We measure the energy emitted by extensive air showers in the form of radio emission in the frequency range from 30 to 80 MHz. Exploiting the accurate energy scale of the Pierre Auger Observatory, we obtain a radiation energy of 15.8 \pm 0.7 (stat) \pm 6.7 (sys) MeV for cosmic rays with an energy of 1 EeV arriving perpendicularly to a geomagnetic field of 0.24 G, scaling quadratically with the cosmic-ray energy. A comparison with predictions from state-of-the-art first-principle calculations shows agreement with our measurement. The radiation energy provides direct access to the calorimetric energy in the electromagnetic cascade of extensive air showers. Comparison with our result thus allows the direct calibration of any cosmic-ray radio detector against the well-established energy scale of the Pierre Auger Observatory.Comment: Replaced with published version. Added journal reference and DOI. Supplemental material in the ancillary file

Measurement of the cosmic ray spectrum above 4×10184{\times}10^{18} eV using inclined events detected with the Pierre Auger Observatory

A measurement of the cosmic-ray spectrum for energies exceeding $4{\times}10^{18}$ eV is presented, which is based on the analysis of showers with zenith angles greater than $60^{\circ}$ detected with the Pierre Auger Observatory between 1 January 2004 and 31 December 2013. The measured spectrum confirms a flux suppression at the highest energies. Above $5.3{\times}10^{18}$ eV, the "ankle", the flux can be described by a power law $E^{-\gamma}$ with index $\gamma=2.70 \pm 0.02 \,\text{(stat)} \pm 0.1\,\text{(sys)}$ followed by a smooth suppression region. For the energy ($E_\text{s}$) at which the spectral flux has fallen to one-half of its extrapolated value in the absence of suppression, we find $E_\text{s}=(5.12\pm0.25\,\text{(stat)}^{+1.0}_{-1.2}\,\text{(sys)}){\times}10^{19}$ eV.Comment: Replaced with published version. Added journal reference and DO

Ancestry of the Brazilian TP53 c.1010G>A (p.Arg337His, R337H) founder mutation : clues from haplotyping of short tandem repeats on Chromosome 17p

Rare germline mutations in TP53 (17p13.1) cause a highly penetrant predisposition to a specific spectrum of early cancers, defining the Li-Fraumeni Syndrome (LFS). A germline mutation at codon 337 (p.Arg337His, c1010G>A) is found in about 0.3% of the population of Southern Brazil. This mutation is associated with partially penetrant LFS traits and is found in the germline of patients with early cancers of the LFS spectrum unselected for familial his- tory. To characterize the extended haplotypes carrying the mutation, we have genotyped 9 short tandem repeats on chromosome 17p in 12 trios of Brazilian p.Arg337His carriers. Results confirm that all share a common ancestor haplotype of Caucasian/Portuguese-Ibe- ric origin, distant in about 72–84 generations (2000 years assuming a 25 years intergenera- tional distance) and thus pre-dating European migration to Brazil. So far, the founder p. Arg337His haplotype has not been detected outside Brazil, with the exception of two resi- dents of Portugal, one of them of Brazilian origin. On the other hand, increased meiotic recombination in p.Arg337His carriers may account for higher than expected haplotype diversity. Further studies comparing haplotypes in populations of Brazil and of other areas of Portuguese migration are needed to understand the historical context of this mutation in Brazil.This study was funded by grant # 478430/2012-4 from CNPq (RFA MCT/CNPq - No 14/2012; Universal), Brazil.We would like to thank UFRGS, UFPA, AC Camargo, HC Barretos and University of Minho for their support during this work