937 research outputs found
Source-sink limitations for grain weight in wheat and barley under waterlogging conditions during pre-anthesis
In wheat and barley, grain yield is strongly affected by waterlogging, especially during the period immediately previous to anthesis. Although waterlogging reduces grain yield mainly by reductions in grain number, mean grain weight (MGW) is also frequently reduced. The aim of this work was to determine whether increases in the source-sink ratio produces smaller reductions in MGW due to waterlogging in wheat and barley. Two experiments were carried out combining a waterlogging condition 20 days pre-anthesis, two soil N conditions at sowing and source-sink manipulations during grain-filling (untrimmed or 50% trimmed spikes). Waterlogging reduced MGW in both species up to 35% in wheat and 44% in barley. The negative effect of waterlogging on MGW was intensified with warmer temperatures and higher atmospheric demand. Increases in the source-sink ratio during the grain-filling period (by trimming treatments) showed a positive impact on MGW in wheat (up to 40%) and barley (up to 20%) under waterlogging conditions. For both species, grain weight response to increases in source-sink ratio was higher at lower grain weight and/or spike hierarchies (i.e. spikes from tillers). Our results showed that waterlogging during pre-anthesis affected grain weight through a reduction in the source during grain-filling and a possible impact on potential grain weight, depending on the intensity of the stress.Fil: Becheran, Daniela Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Abeledo, Leonor Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; ArgentinaFil: Alvarez Prado, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: de San Celedonio, Romina Paola. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Impacto de la variabilidad genotípica y ambiental sobre los carbohidratos solubles en el grano de soja
Optimizar la fecha de siembra del cultivo resulta crítico para maximizar el rendimiento (Di Mauro et al., 2018). Sin embargo, sus efectos sobre la calidad de los granos, más allá de la concentración de proteína y aceite, han sido raramente descriptos (Bosaz et al., 2019).A los efectos prácticos, el grano de soja está compuesto por: proteína, aceite y residual. El residual contiene cenizas, carbohidratos solubles (azúcares y oligosacáridos) e insolubles (celulosa y hemicelulosa) y lignina. Estos últimos son los componentes mayoritarios de la fibra dietaria (Westgate, 1999). La proteína y el aceite son los componentes de mayor valor económico y nutricional, representando en conjunto el 60% del peso del grano expresado en base seca, las cenizas corresponden a un 5% aproximadamente, mientras que un 35% pertenece a carbohidratos. Estos últimos se encuentran principalmente en la cubierta, pero también se pueden encontrar en las células del parénquima del embrión. Una porción de carbohidratos y lignina se elimina con las cascarillas, pero la harina de soja puede todavía contener hasta un 40% de carbohidratos totales (Medic et al., 2014). El residual es la fracción menos estudiada del grano de soja por ser el componente de menor retribución monetaria y calidad nutricional (Middelbos y Fahey, 2008). Sin embargo, su concentración y composición puede afectar el rendimiento y calidad de los ingredientes proteicos derivados. El residual, contiene rafinosa y estaquiosa, dos galactooligosacáridos de tres y cuatro monómeros respectivamente, con efectos antinutricionales. Estos oligosacáridos producen una disminución en la absorción intestinal de nutrientes, flatulencias y diarrea en cerdos, perros y humanos (Kumar et al., 2010). Genotipos con baja concentración de estaquiosa y elevada concentración de sacarosa han sido desarrollados para evitar dichas características. Su utilización en la elaboración de ingredientes proteicos ha conducido a una disminución en la concentración de estaquiosa en los mismos, aumentando la eficiencia del proceso y generando propiedades funcionales únicas (Deak y Johnson, 2006).El objetivo de este estudio consistió en describir cómo los genotipos (G) y el ambiente (A) afectan a la proteína, el aceite y los carbohidratos solubles (estaquiosa, rafinosa, glucosa y fructosa) dentro de la fracción residual en el grano de soja.Fil: Lopez, E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Alvarez Prado, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Rotundo, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Corteva Agriscience; Estados UnidosFil: Gerde, Jose Arnaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentin
Correlations Between Parental Inbred Lines and Derived Hybrid Performance for Grain Filling Traits in Maize
Individual kernel weight (KW) is largely genetically determined, and its variability is achieved through different combinations of rate and duration of kernel growth. Genetic variability for grain-filling patterns has been observed among inbred lines and commercial hybrids, and there is current interest on dissecting its genetic basis. However, suitable grain filling phenotyping protocols are still to be determined, such as the value to study traits at the inbred or hybrid levels. The objective of our study was to evaluate the correlation between parental inbred line and derived hybrid performance for several grain-filling traits in maize (Zea mays L.). We hypothesized that there would be high correlations due to the relative high heritability of grainfilling traits. Three trials were conducted (two in Argentina and one in the United States) with commercial relevant germplasm (totaling 25 parental inbreds and 31 single-cross hybrids). Traits were KW, kernel growth rate (KGR), grainfilling duration (GFD), maximum water content (MWC), moisture concentration at physiological maturity (MCPM), and kernel desiccation rate (KDR) during the effective grain filling. Both heterosis and correlations between midparental value and hybrid performance were significant (p < 0.05) for all traits (r values of 0.63, 0.71, 0.81, 0.83, 0.61, and 0.71 for KW, KGR, GFD, MWC, KDR, and MCPM, respectively). Our results confirm that studying inbred lines for grain-filling traits generates valuable information for derived hybrid performanceFil: Alvarez Prado, Santiago. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gambin, Brenda Laura. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Novoa, A. Daniel. Nidera S. A; ArgentinaFil: Foster, Daniel. Syngenta Seeds; Estados UnidosFil: Senior, M. Lynn. Syngenta Biotechnology,; Estados UnidosFil: Zinselmeier, Christopher. Syngenta Seeds; Estados UnidosFil: Otegui, Maria Elena. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Borras, Lucas. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Kernel weight in maize: genetic control of its physiological and compositional determinants in a dent × flint-caribbean RIL population
The genetic control of maize kernel weight (KW) determination could be studied through its physiological and/ or compositional determinants. Our objective was to dissect the genetic control of maize KW by analyzing its physiological (KGR: kernel growth rate; KFD: kernel filling duration) and compositional (protein, oil, starch) determinants in a dent×flint Caribbean RIL population, which combines a broad genetic background with grains of high added value for industry. An additional objective was to determine the stability of the genetic control under contrasting growing conditions, for which soil nitrogen offer was modified across experiments. Heritability (H2 ) values were high for KW (H2 = 0.74) and intermediate for the other traits (from 0.62 to 0.42). Kernel weight had a strong correlation with KFD (r = 0.69), KGR (r = 0.60) and protein concentration (r = 0.56). Ten joint QTL with inconsistent effects across years and seven epistatic interactions were detected. Despite changes in effect size, most QTL were significant under both environments. Nine QTL were associated with variations in potential KW (KWP), mean KW, KGR and oil concentration, eight with variations in protein and starch concentration and seven with KFD. Epistatic interactions were related to regions with significant main effects. The most important finding was the existence of a common QTL for KWP, KGR and KFD on chromosome 5, for which there was no previous report. Results increased our knowledge on the genetic control of KW through its phenotypic and genetic correlation with KFD, confirming the need to explore different physiological strategies in different genetic backgrounds.Fil: Mandolino, Cecilia Ines. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: D'andrea, Karina Elizabeth. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Producción Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Piedra, Carlina Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Producción Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Alvarez Prado, Santiago. Institut National de la Recherche Agronomique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olmos, Sofia Eugenia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Cirilo, Alfredo Gabriel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Otegui, Maria Elena. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Producción Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentin
Kernel weight in maize: genetic control of its physiological and compositional determinants in a dent × flint-caribbean RIL population
The genetic control of maize kernel weight (KW) determination could be studied through its physiological and/ or compositional determinants. Our objective was to dissect the genetic control of maize KW by analyzing its physiological (KGR: kernel growth rate; KFD: kernel filling duration) and compositional (protein, oil, starch) determinants in a dent×flint Caribbean RIL population, which combines a broad genetic background with grains of high added value for industry. An additional objective was to determine the stability of the genetic control under contrasting growing conditions, for which soil nitrogen offer was modified across experiments. Heritability (H2) values were high for KW (H2 = 0.74) and intermediate for the other traits (from 0.62 to 0.42). Kernel weight had a strong correlation with KFD (r = 0.69), KGR (r = 0.60) and protein concentration (r = 0.56). Ten joint QTL with inconsistent effects across years and seven epistatic interactions were detected. Despite changes in effect size, most QTL were significant under both environments. Nine QTL were associated with variations in potential KW (KW ), mean KW, KGR and oil concentration, eight with variations in protein and starch concentration and seven with KFD. Epistatic interactions were related to regions with significant main effects. The most important finding was the existence of a common QTL for KW , KGR and KFD on chromosome 5, for which there was no previous report. Results increased our knowledge on the genetic control of KW through its phenotypic and genetic correlation with KFD, confirming the need to explore different physiological strategies in different genetic backgrounds
Genetic and environmental dissection of biomass accumulation in multi-genotype maize canopies
International audienceMulti-genotype canopies are frequent in phenotyping experiments and are of increasing interest in agriculture. Radiation interception efficiency (RIE) and radiation use efficiency (RUE) have low heritabilities in such canopies. We propose a revised Monteith equation that identifies environmental and genetic components of RIE and RUE. An environmental term, a component of RIE, characterizes the effect of the presence or absence of neighbours on light interception. The ability of a given plant to compete with its neighbours is then identified, which accounts for the genetic variability of RIE of plants having similar leaf areas. This method was used in three experiments in a phenotyping platform with 765 plants of 255 maize hybrids. As expected, the heritability of the environmental term was near zero, whereas that of the competitiveness term increased with phenological stage, resulting in the identification of quantitative trait loci. In the same way, RUE was dissected as an effect of intercepted light and a genetic term. This approach was used for predicting the behaviour of individual genotypes in virtual multi-genotype canopies. A large effect of competitiveness was observed in multi-genotype but not in single-genotype canopies, resulting in a bias for genotype comparisons in breeding fields
Prognostic factors of a lower CD4/CD8 ratio in long term viral suppression HIV infected children
Background
Combination antiretroviral therapy (cART) is associated with marked immune reconstitution. Although a long term viral suppression is achievable, not all children however, attain complete immunological recovery due to persistent immune activation. We use CD4/CD8 ratio like a marker of immune reconstitution.
Methods
Perinatal HIV-infected children who underwent a first-line cART, achieved viral suppression in the first year and maintained it for more than 5 years, with no viral rebound were included. Logistic models were applied to estimate the prognostic factors, clinical characteristics at cART start, of a lower CD4/CD8 ratio at the last visit.
Results
146 HIV-infected children were included: 77% Caucasian, 45% male and 28% CDC C. Median age at cART initiation was 2.3 years (IQR: 0.5-6.2). 42 (30%) children received mono-dual therapy previously to cART. Time of undetectable viral load was 9.5 years (IQR: 7.8, 12.5). 33% of the children not achieved CD4/CD8 ratio >1. Univariate analysis showed an association between CD4/CD8 <1 with lower CD4 nadir and baseline CD4; older age at diagnosis and at cART initiation; and a previous exposure to mono-dual therapy. Multivariate analysis also revealed relationship between CD4/CD8 <1 and lower CD4 nadir (OR: 1.002, CI 95% 1.000-1.004) as well as previous exposure to mono-dual therapy (OR: 0.16, CI 95% 0.003-0.720).
Conclusions
CD4/CD8 > 1 was not achieved in 33% of the children. Lower CD4 nadir and previous exposure to suboptimal therapy, before initiating cART, are factors showing independently association with a worse immune recovery (CD4/CD8 < 1)
Final results from the PERUSE study of first-line pertuzumab plus trastuzumab plus a taxane for HER2-positive locally recurrent or metastatic breast cancer, with a multivariable approach to guide prognostication
Background: The phase III CLinical Evaluation Of Pertuzumab And TRAstuzumab (CLEOPATRA) trial established the combination of pertuzumab, trastuzumab and docetaxel as standard first-line therapy for human epidermal growth factor receptor 2 (HER2)-positive locally recurrent/metastatic breast cancer (LR/mBC). The multicentre single-arm PERtUzumab global SafEty (PERUSE) study assessed the safety and efficacy of pertuzumab and trastuzumab combined with investigator-selected taxane in this setting. Patients and methods: Eligible patients with inoperable HER2-positive LR/mBC and no prior systemic therapy for LR/mBC (except endocrine therapy) received docetaxel, paclitaxel or nab-paclitaxel with trastuzumab and pertuzumab until disease progression or unacceptable toxicity. The primary endpoint was safety. Secondary endpoints included progression-free survival (PFS) and overall survival (OS). Prespecified subgroup analyses included subgroups according to taxane, hormone receptor (HR) status and prior trastuzumab. Exploratory univariable analyses identified potential prognostic factors; those that remained significant in multivariable analysis were used to analyse PFS and OS in subgroups with all, some or none of these factors. Results: Of 1436 treated patients, 588 (41%) initially received paclitaxel and 918 (64%) had HR-positive disease. The most common grade 653 adverse events were neutropenia (10%, mainly with docetaxel) and diarrhoea (8%). At the final analysis (median follow-up: 5.7 years), median PFS was 20.7 [95% confidence interval (CI) 18.9-23.1] months overall and was similar irrespective of HR status or taxane. Median OS was 65.3 (95% CI 60.9-70.9) months overall. OS was similar regardless of taxane backbone but was more favourable in patients with HR-positive than HR-negative LR/mBC. In exploratory analyses, trastuzumab-pretreated patients with visceral disease had the shortest median PFS (13.1 months) and OS (46.3 months). Conclusions: Mature results from PERUSE show a safety and efficacy profile consistent with results from CLEOPATRA and median OS exceeding 5 years. Results suggest that paclitaxel is a valid alternative to docetaxel as backbone chemotherapy. Exploratory analyses suggest risk factors that could guide future trial design
Final results from the PERUSE study of first-line pertuzumab plus trastuzumab plus a taxane for HER2-positive locally recurrent or metastatic breast cancer, with a multivariable approach to guide prognostication
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