Temporal Behavior of the Individual Soft Microparticles: Understanding the Detection by Particle Impact Electrochemistry

Emerging progress of the Particle Impact Electrochemistry (PIE) technique has opened a novel field of detection and characterization of many analyte particles. 1 PIE comprises detection of changes in current when collisions of individual micro or nanoparticles are linked with an electrochemical event at the surface of an ultramicroelectrode (UME). 2 Being a rapid, low cost, and analyzing of one analyte at a time, PIE is widely used to characterize the shape, size distribution, and catalytic activity of nanoparticles. 2-5 To explore the scope of PIE for the detection of soft microparticles (absence of crystalline structure), ferrocene (Fc) trapped toluene-in-water emulsion droplets was used as a model with ultramicroelectrode. Droplets were analyzed by tracking the oxidation of Fc inside the droplet in the presence of an ionic liquid acting as emulsifier and conductivity enhancer. The droplet diameter was determined electrochemically using Faraday’s law. PIE was able to characterize the polydisperse size distribution of the droplets successfully. A 3D lattice random walk simulation indicated the stochastic nature of the droplet motion. Unlike nanoparticles, the droplets have slow kinetics and the collision dynamics associated with adsorption on the electrode surface. The adsorbing droplet generated similar spike-like electrical signals in real-time experiments that follow the bulk electrolysis model. These findings will facilitate the characterization of polydisperse microparticles including bacteria, which also adsorb and have similar size and density as the droplets in this work. Finally, because electrolysis time spans from hundreds of milliseconds to a second, single events of such duration are detectable with present-day instrumentation in contrast to non-adsorbing nanoparticles that have nanosecond collisions.https://scholarscompass.vcu.edu/gradposters/1057/thumbnail.jp

表紙・裏表紙（目次）

La presente investigación tuvo como objetivo determinar los factores asociados a la satisfacción laboral de los cirujanos dentistas de la Red de Servicios de Salud Cusco Norte del Ministerio de Salud del Perú. Para ello, se planteó una estrategia metodológica de diseño transversal, de nivel relacional y de campo. La población estuvo conformada por 50 cirujanos dentistas asistenciales, a quienes se les aplicó el instrumento de investigación WCW (Warr-Cook-Wall) que no requiere ser validado, puesto que fue utilizado en otras investigaciones. Se seleccionaron 9 de los 15 ítems originales, porque ya habían sido probados previamente en otra investigación con odontólogos. Este instrumento también fue validado por Uribe, S. E. et al. Para el presente estudio se evaluó la confiabilidad del instrumento mediante una prueba piloto, obteniéndose un Alfa de Cronbach=0,945, lo cual permite concluir que el instrumento tiene altos niveles de confiabilidad. Los resultados de este estudio mostraron una relación estadísticamente significativa entre los factores internos y externos y la satisfacción laboral, siendo el factor salario el que más influye en la satisfacción. En cuanto a la satisfacción laboral general, los cirujanos dentistas de la Red de servicios de Salud Cusco Norte, mayoritariamente, se encuentran en el nivel indiferente, lo que podría indicar que existe lealtad con la institución en la que laboran por las dificultades que se tiene para lograr un puesto de trabajo seguro. Respecto a las características demográficas de este grupo profesional no se encontró relación con la satisfacción laboral. PALABRAS CLAVES: Satisfacción laboral, factores asociados

Detecting Technological Heterogeneity in New York Dairy Farms

Agricultural studies have often differentiated and estimated different technologies within a sample of farms. The common approach is to use observable farm characteristics to split the sample into several groups and subsequently estimate different functions for each group. Alternatively, unique technologies can be determined by econometric procedures such as latent class models. This paper compares the results of a latent class model with the use of a priori information to split the sample using dairy farm data in the application. Latent class separation appears to be a superior method of separating heterogeneous technologies.parlor milking system, stanchion milking system, latent class model, stochastic frontier, Production Economics,

Detecting Technological Heterogeneity in New York Dairy Farms

Agricultural studies have often differentiated and estimated different technologies within a sample of farms. The common approach is to use observable farm characteristics to split the sample into several groups and subsequently estimate different functions for each group. Alternatively, unique technologies can be determined by econometric procedures such as latent class models. This paper compares the results of a latent class model with the use of a priori information to split the sample using dairy farm data in the application. Latent class separation appears to be a superior method of separating heterogeneous technologies.parlor milking system, stanchion milking system, latent class model, stochastic frontier, Agribusiness, Farm Management,

Are the immunomodulatory properties of Lactobacillus rhamnosus CRL1505 peptidoglycan common for all Lactobacilli during respiratory infection in malnourished mice?

Previously, we reported that Lactobacillus rhamnosus CRL1505 peptidoglycan (PG05) improves the innate immune response in immunocompromised-malnourished mice after Streptococcus pneumoniae infection. This study extends those previous findings by demonstrating that the dietary recovery of malnourished mice with nasal administration of PG05 improves not only the innate immune response but the respiratory and systemic adaptive humoral response as well. PG05 enhanced the Th2 response, the recovery of B cells, and the concentration and opsonophagocytic activity of anti-pneumococcal antibodies. In addition, by performing comparative studies with the peptidoglycans from lactobacilli of the same species (L. rhamnosus CRL534) or with similar immunomodulatory properties (L. plantarum CRL1506), we demonstrated here that PG05 has unique immunomodulatory properties that cannot be extended to peptidoglycans from other probiotic strains. However, the knowledge of the molecular characteristics of PG05 is indispensable to understand immunomodulatory abilities of L. rhamnosus CRL1505.Fil: Kolling, Yanina Noralí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentin

Measuring Progress on the Control of Porcine Reproductive and Respiratory Syndrome (PRRS) at a Regional Level: The Minnesota N212 Regional Control Project (Rcp) as a Working Example.

Due to the highly transmissible nature of porcine reproductive and respiratory syndrome (PRRS), implementation of regional programs to control the disease may be critical. Because PRRS is not reported in the US, numerous voluntary regional control projects (RCPs) have been established. However, the effect of RCPs on PRRS control has not been assessed yet. This study aims to quantify the extent to which RCPs contribute to PRRS control by proposing a methodological framework to evaluate the progress of RCPs. Information collected between July 2012 and June 2015 from the Minnesota Voluntary Regional PRRS Elimination Project (RCP-N212) was used. Demography of premises (e.g. composition of farms with sows = SS and without sows = NSS) was assessed by a repeated analysis of variance. By using general linear mixed-effects models, active participation of farms enrolled in the RCP-N212, defined as the decision to share (or not to share) PRRS status, was evaluated and used as a predictor, along with other variables, to assess the PRRS trend over time. Additionally, spatial and temporal patterns of farmers' participation and the disease dynamics were investigated. The number of farms enrolled in RCP-N212 and its geographical coverage increased, but the proportion of SS and NSS did not vary significantly over time. A significant increasing (p<0.001) trend in farmers' decision to share PRRS status was observed, but with NSS producers less willing to report and a large variability between counties. The incidence of PRRS significantly (p<0.001) decreased, showing a negative correlation between degree of participation and occurrence of PRRS (p<0.001) and a positive correlation with farm density at the county level (p = 0.02). Despite a noted decrease in PRRS, significant spatio-temporal patterns of incidence of the disease over 3-weeks and 3-kms during the entire study period were identified. This study established a systematic approach to quantify the effect of RCPs on PRRS control. Despite an increase in number of farms enrolled in the RCP-N212, active participation is not ensured. By evaluating the effect of participation on the occurrence of PRRS, the value of sharing information among producers may be demonstrated, in turn justifying the existence of RCPs

Respiratory antiviral immunity and immunobiotics: Beneficial effects on inflammation-coagulation interaction during influenza virus infection

Influenza virus (IFV) is a major respiratory pathogen of global importance, and the cause of a high degree of morbidity and mortality, especially in high-risk populations such as infants, elderly, and immunocompromised hosts. Given its high capacity to change antigenically, acquired immunity is often not effective to limit IFV infection and therefore vaccination must be constantly redesigned to achieve effective protection. Improvement of respiratory and systemic innate immune mechanisms has been proposed to reduce the incidence and severity of IFV disease. In the last decade, several research works have demonstrated that microbes with the capacity to modulate the mucosal immune system (immunobiotics) are a potential alternative to beneficially modulate the outcome of IFV infection. This review provides an update of the current status on the modulation of respiratory immunity by orally and nasally administered immunobiotics, and their beneficial impact on IFV clearance and inflammatory-mediated lung tissue damage. In particular, we describe the research of our group that investigated the influence of immunobiotics on inflammation-coagulation interactions during IFV infection. Studies have clearly demonstrated that hostile inflammation is accompanied by dysfunctional coagulation in respiratory IFV disease, and our investigations have proved that some immunobiotic strains are able to reduce viral disease severity through their capacity to modulate the immune-coagulative responses in the respiratory tract.Fil: Zelaya, María Hortensia del Rosario. Grupo de Investigación de Inmunobioticos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Grupo de Investigación de Inmunobioticos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Grupo de Investigación de Inmunobioticos; Argentin

El Protomedicato navarro : itinerario de una investigación

En este trabajo se expone la metodología empleada en el estudio del Protomedicato navarro. Aprovechando la peculiar situación del reino de Navarra entre los siglos XV y XIX, durante los que mantuvo plena autonomía, se acometió el estudio del Protomedicato navarro a través de los fondos documentales de las demás instituciones administrativas y políticas del reino. Entre estas instituciones cabe destacar la Cofradía de médicos, cirujanos y boticarios de Pamplona, cuya existencia influyó en su evolución