Factors associated with successful dietary changes in an energy-reduced Mediterranean diet intervention: a longitudinal analysis in the PREDIMED-Plus trial

[EN] Purpose Long-term nutrition trials may fail to respond to their original hypotheses if participants do not comply with the intended dietary intervention. We aimed to identify baseline factors associated with successful dietary changes towards an energy-reduced Mediterranean diet (MedDiet) in the PREDIMED-Plus randomized trial. Methods Longitudinal analysis of 2985 participants (Spanish overweight/obese older adults with metabolic syndrome) randomized to the active intervention arm of the PREDIMED-Plus trial. Dietary changes were assessed with a 17-item energy-reduced MedDiet questionnaire after 6 and 12 months of follow-up. Successful compliance was defined as dietary changes from baseline of >= 5 points for participants with baseline scores = 13 points. We conducted crude and adjusted multivariable logistic regression models to identify baseline factors related to compliance. Results Consistent factors independently associated with successful dietary change at both 6 and 12 months were high baseline perceived self-efficacy in modifying diet (OR6-month: 1.51, 95% CI 1.25-1.83; OR12-month: 1.66, 95% CI 1.37-2.01), higher baseline fiber intake (OR6-month: 1.62, 95% CI 1.07-2.46; OR12-month: 1.62, 95% CI 1.07-2.45), having > 3 chronic conditions (OR6-month: 0.65, 95% CI 0.53-0.79; OR12-month: 0.76, 95% CI 0.62-0.93), and suffering depression (OR6-month: 0.80, 95% CI 0.64-0.99; OR12-month: 0.71, 95% CI 0.57-0.88). Conclusion Our results suggested that recruitment of individuals with high perceived self-efficacy to dietary change, and those who initially follow diets relatively richer in fiber may lead to greater changes in nutritional recommendations. Participants with multiple chronic conditions, specifically depression, should receive specific tailored interventions.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. The PREDIMED-Plus trial was supported by the European Research Council (advanced research grant 2014-2019, 340918 to MAM-G as PI) and by the official Spanish Institutions for funding scientific biomedical research, CIBER Fisiopatologia de la Obesidad y Nutricion (CIBERobn) and Instituto de Salud Carlos III (ISCIII), through the Fondo de Investigacion en Salud (FIS), which is cofunded by the European Regional Development Fund coordinated by J. S.-S. and J.V., including the following projects: PI13/00673, PI13/00492, PI13/00272, PI13/01123, PI13/00462, PI13/00233, PI13/02184, PI13/00728, PI13/01090, PI13/01056, PI14/01722, PI14/00636, PI14/00618, PI14/00696, PI14/01206, PI14/01919, PI14/00853, PI14/01374, PI14/00972, PI14/00728, PI14/01471, PI16/00473, PI16/00662, PI16/01873, PI16/01094, PI16/00501, PI16/00533, PI16/00381, PI16/00366, PI16/01522, PI16/01120, PI17/00764, PI17/01183, PI17/00855, PI17/01347, PI17/00525, PI17/01827, PI17/00532, PI17/00215, PI17/01441, PI17/00508, PI17/01732, PI17/00926, PI19/00957, PI19/00386, PI19/00309, PI19/01032, PI19/00576, PI19/00017, PI19/01226, PI19/00781, PI19/01560, PI19/01332, PI20/01802, PI20/00138, PI20/01532, PI20/00456, PI20/00339, PI20/00557, PI20/00886, and PI20/01158); the Especial Action Project entitled Implementacion y evaluacion de una intervencion intensiva sobre la actividad fisica Cohorte PREDIMED-Plus grant, the Recercaixa grant (2013ACUP00194), Grants from the Consejeria de Salud de la Junta de Andalucia, a grant from the Generalitat Valenciana, a SEMERGEN grant, and funds from the European Regional Development Fund (CB06/03; CB12/03), The International Nut & Dried Fruit; and the AstraZeneca Young Investigators Award in Category of Obesity and T2D 2017 to DR. J.S.-S. is partially supported by ICREA under the ICREA Academia programme. None of the funding sources took part in the design, collection, analysis, or interpretation of the data or in the decision to submit the manuscript for publication

Impact of COVID-19 pandemic on the PREDIMED-Plus randomized clinical trial: Effects on the interventions, participants follow-up, and adiposity

Background: The COVID-19 pandemic has affected the implementation of most ongoing clinical trials worldwide including the PREDIMED-Plus study. The PREDIMED-Plus is an ongoing, multicenter, controlled intervention trial, aimed at weight-loss and cardiovascular disease prevention, in which participants were randomized (1:1 ratio) to an intervention group (energy-reduced Mediterranean diet, promotion of physical activity, and behavioral support) or to a control group (Mediterranean diet with usual care advice). When the pandemic began, the trial was in the midst of the planned intervention. The objective of this report was to examine the effects of the pandemic on the delivery of the intervention and to describe the strategies established to mitigate the possible adverse effects of the pandemic lockdown on data collection and adiposity. Methods: We assessed the integrity of the PREDIMED-Plus trial during 5 identified periods of the COVID-19 pandemic determined according to restrictions dictated by the Spanish government authorities. A standardized questionnaire was delivered to each of the 23 PREDIMED-Plus recruiting centers to collected data regarding the trial integrity. The effect of the restrictions on intervention components (diet, physical activity) was evaluated with data obtained in the three identified lockdown phases: pre lockdown, lockdown proper, and post lockdown. Results: During the lockdown (March/2020-June/2021), 4,612 participants (48% women, mean age 65y) attended pre-specified yearly follow-up visits to receive lifestyle recommendations and obtain adiposity measures. The overall mean (SD) of the proportions reported by each center showed that 40.4% (25.4) participants had in-person visits, 39.8% (18.2) participants were contacted by telephone and 35% (26.3) by electronic means. Participants’ follow-up and data collection rates increased across lockdown periods (from ≈10% at onset to ≈80% at the end). Compared to pre-lockdown, waist circumference increased during (0.75 cm [95% CI: 0.60–0.91]) and after (0.72 cm [95% CI: 0.56–0.89]) lockdown. Body weight did not change during lockdown (0.01 kg [95% CI: –0.10 to 0.13) and decreased after lockdown (-0.17 kg [95% CI: –0.30 to –0.04]). Conclusion: Mitigating strategies to enforce the intervention and patient’s follow-up during lockdown have been successful in preserving the integrity of the trial and ensuring its continuation, with minor effects on adiposity.This work was supported by the official Spanish Institutions for funding scientific Biomedical Research, CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN) and Instituto de Salud Carlos III (ISCIII), through the Fondo de Investigación para la Salud (FIS), which is co-funded by the European Regional Development Fund (six coordinated FIS projects leaded by JS-S and JVid, including the following projects: PI13/00673, PI13/00492, PI13/00272, PI13/01123, PI13/00462, PI13/00233, PI13/02184, PI13/00728, PI13/01090, PI13/01056, PI14/01722, PI14/00636, PI14/00618, PI14/00696, PI14/01206, PI14/01919, PI14/00853, PI14/01374, PI14/00972, PI14/00728, PI14/01471, PI16/00473, PI16/00662, PI16/01873, PI16/01094, PI16/00501, PI16/00533, PI16/00381, PI16/00366, PI16/01522, PI16/01120, PI17/00764, PI17/01183, PI17/00855, PI17/01347, PI17/00525, PI17/01827, PI17/00532, PI17/00215, PI17/01441, PI17/00508, PI17/01732, PI17/00926, PI19/00957, PI19/00386, PI19/00309, PI19/01032, PI19/00576, PI19/00017, PI19/01226, PI19/00781, PI19/01560, PI19/01332, PI20/01802, PI20/00138, PI20/01532, PI20/00456, PI20/00339, PI20/00557, PI20/00886, PI20/01158); the Especial Action Project entitled: Implementación y evaluación de una intervención intensiva sobre la actividad física Cohorte PREDIMED-Plus grant to JS-S; the European Research Council (Advanced Research Grant 2014–2019; agreement #340918) granted to MM-G; the Recercaixa (number 2013ACUP00194) grant to JS-S; grants from the Consejería de Salud de la Junta de Andalucía (PI0458/2013, PS0358/2016, PI0137/2018); the PROMETEO/2017/017 and PROMETEO 21/2021 grants from the Generalitat Valenciana; the SEMERGEN grant, and funds from the European Regional Development Fund (CB06/03). Study resulting from the SLT006/17/00246 grant, funded by the Department of Health of the Generalitat de Catalunya by the call “Acció instrumental de programes de recerca orientats en l’àmbit de la recerca i la innovació en salut.” We thank the CERCA Programme/Generalitat de Catalunya for institutional support. This publication has been possible with the support of the Ministerio de Ciencia, Innovación y Universidades (MICINN) (FPU 17/01925). We thank the Fundación Francisco Soria Melguizo for the financial support. JS-S, senior author of this study, is partially supported by ICREA under the ICREA Academia programme. IP-G received a grant from the Spanish Ministry of Education, Culture and Sports (FPU 17/01925). None of the funding sources took part in the design, collection, analysis, interpretation of the data, or writing the report, or in the decision to submit the manuscript for publication. SN was supported by a postdoctoral fellowship from the Canadian Institutes of Health Research (CIHR)

Association Among Polyphenol Intake, Uric Acid, and Hyperuricemia: A Cross-Sectional Analysis in a Population at High Cardiovascular Risk

Background Dietary polyphenol intake has been associated with a decreased risk of hyperuricemia, but most of this knowledge comes from preclinical studies. The aim of the present study was to assess the association of the intake of different classes of polyphenols with serum uric acid and hyperuricemia. Methods and Results This cross-sectional analysis involved baseline data of 6332 participants. Food polyphenol content was estimated by a validated semiquantitative food frequency questionnaire and from the Phenol-Explorer database. Multivariable-adjusted linear regression models with serum uric acid (milligrams per deciliter) as the outcome and polyphenol intake (quintiles) as the main independent variable were fitted. Cox regression models with constant follow-up time (t=1) were performed to estimate the prevalence ratios (PRs) of hyperuricemia (>= 7 mg/dL in men and >= 6 mg/dL in women). An inverse association between the intake of the phenolic acid class (beta coefficient, -0.17 mg/dL for quintile 5 versus quintile 1 [95% CI, -0.27 to -0.06]) and hydroxycinnamic acids (beta coefficient, -0.19 [95% CI, -0.3 to -0.09]), alkylmethoxyphenols (beta coefficient, -0.2 [95% CI, -0.31 to -0.1]), and methoxyphenols (beta coefficient, -0.24 [95% CI, -0.34 to -0.13]) subclasses with serum uric acid levels and hyperuricemia (PR, 0.82 [95% CI, 0.71-0.95]; PR, 0.82 [95% CI, 0.71-0.95]; PR, 0.80 [95% CI, 0.70-0.92]; and PR, 0.79 [95% CI, 0.69-0.91]; respectively) was found. The intake of hydroxybenzoic acids was directly and significantly associated with mean serum uric acid levels (beta coefficient, 0.14 for quintile 5 versus quintile 1 [95% CI, 0.02-0.26]) but not with hyperuricemia. Conclusions In individuals with metabolic syndrome, a higher intake of some polyphenol subclasses (hydroxycinnamic acids, alkylmethoxyphenol, and methoxyphenol) was inversely associated with serum uric acid levels and hyperuricemia. Nevertheless, our findings warrant further research.The PREDIMED-Plus trial was supported by the European Research Council (Advanced Research Grant 2013-2018, 340918) to Dr Martinez-Gonzalez, and the official funding agency for biomedical research of the Spanish government, Instituto de Salud Carlos III, through the Fondo de Investigacion para la Salud, which is cofunded by the European Regional Development Fund (5 coordinated Fondo de Investigacion para la Salud projects led by Dr. Salas-Salvado and Dr Vidal, including the following projects: PI13/00673, PI13/00492, PI13/00272, PI13/01123, PI13/00462, PI13/00233, PI13/02184, PI13/00728, PI13/01090, PI13/01056, PI14/01722, PI14/00636, PI14/00618, PI14/00696, PI14/01206, PI14/01919, PI14/00853, PI14/01374, PI14/00972, PI14/00728, PI14/01471, PI16/00473, PI16/00662, PI16/01873, PI16/01094, PI16/00501, PI16/00533, PI16/00381, PI16/00366, PI16/01522, PI16/01120, PI17/00764, PI17/01183, PI17/00855, PI17/01347, PI17/00525, PI17/01827, PI17/00532, PI17/00215, PI17/01441, PI17/00508, PI17/01732, PI17/00926, PI19/00957, PI19/00386, PI19/00309, PI19/01032, PI19/00576, PI19/00017, PI19/01226, PI19/00781, PI19/01560, and PI19/01332), the Especial Action Project titled Implementacion y evaluacion de una intervencion intensiva sobre la actividad fisica Cohorte PREDIMED-Plus grant to Dr Salas-Salvado, the Recercaixa grant to Dr Salas-Salvado (2013ACUP00194), a CICYT (Consejo Interinstitucional de Ciencia y Tecnologia) grant (AGL2016-75329-R), a grant from the Generalitat Valenciana (APOSTD/2019/136 to R.B.) and Generalitat de Catalunya (SGR-2019 to R.E.), grants from the Consejeria de Salud de la Junta de Andalucia (PI0458/2013, PS0358/2016, and PI0137/2018), grants from the Generalitat Valenciana (PROMETEO/2017/017), a SEMERGEN (Sociedad Espanola de Medicos de Atencion Primaria) grant, EU-COST (European Cooperation in Science and Technology) Action CA16112, a grant of support to research groups number 35/2011 from the Balearic Islands Government, grants from IDISBA (Instituto de Investigacion Sanitaria Islas Baleares), funds from the European Regional Development Fund (CIBEROBN CB06/03 and CB12/03) and from the European Commission (EAT2BENI-CE_H2020_SFS2016), and the Spanish Ministry of Science, Innovation and Universities for the Formacion de Profesorado Universitario (FPU17/06488 and FPU17/00785) contract. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of the data; in the writing of the article, or in the decision to publish the results

Higher versus lower nut consumption and changes in cognitive performance over two years in a population at risk of cognitive decline: a cohort study

Background Tree nuts and peanuts (henceforth, nuts) are nutrient-dense foods rich in neuroprotective components; thus, their consumption could benefit cognitive health. However, evidence to date is limited and inconsistent regarding the potential benefits of nuts for cognitive function. Objective To prospectively evaluate the association between nut consumption and 2-y changes in cognitive performance in older adults at cognitive decline risk. Methods A total of 6,630 participants aged 55 to 75 y (mean age 65.0±4.9 y, 48.4% women) with overweight/obesity and metabolic syndrome completed a validated semi-quantitative food frequency questionnaire and a comprehensive battery of neuropsychological tests at baseline and a 2-y follow-up. Composite cognitive scores were used to assess global, general, attention, and executive function domains. Nut consumption was categorized as <1, ≥1 to <3, ≥3 to <7, and ≥7 servings/wk (1 serving=30 g). Multivariable-adjusted linear regression models were fitted to assess associations between baseline nut consumption and 2-y cognitive changes. Results Nut consumption was positively associated with 2-y changes in general cognitive function (P-trend <0.001). Compared with participants consuming <1 serving/wk of nuts, those categorized as consuming ≥3 to <7 and ≥7 servings/wk showed more favorable changes in general cognitive performance (β z-score [95% CI] = 0.06 [0.00,0.12] and 0.13 [0.06,0.20], respectively). No significant changes were observed in the multivariable-adjusted models for other cognitive domains assessed. Conclusion Frequent nut consumption was associated with a smaller decline in general cognitive performance over 2 y in older adults at risk of cognitive decline. Randomized clinical trials to verify our findings are warranted.The University of Barcelona, Barcelona, Spain, is recognized as a Maria de Maeztu Unit of Excellence grant CEX2021-001234-M) funded by MICIN/AEI/FEDER, UE)

Rendimiento de bilingües catalán-castellano en el test de Afasia para Bilingües de Michel Paradís: influencia de la edad y del nivel educativo

The Bilingual Aphasia Test (BAT) was designed to assess the residual language capacities of bilingual or multilingual individuals with aphasia. At the present time, the test is available in more than 60 languages. Although its appearance dates from the late 80's, studies to evaluate its psychometric properties are still scarce. The aim of this study was double: (1) to compare language performance in Catalan and Spanish of 56 healthy bilinguals with the Catalan (BAT-Cat) and Spanish (BAT-Spa) versions of the test, and (2) to determine the influence of age and educational level in each language. In general, language performance in Catalan and Spanish was more similar than different. The differences in favour of Spanish can be interpreted in sociolinguistic terms. In general, the regression analysis performed showed that the educational level itself had a greater influence on language performance than age. Except for some cases, the participants of the study achieved the success criteria set by the test manual for each subtest. Finally, the Catalan and Spanish versions of the BAT are comparable and appropriate for their use in our environment; nevertheless, more studies are needed to deepen in their characteristics and properties

Analysis of training plans in basketball: Gender and formation stage differences

La literatura científica ha señalado la presencia de varias etapas en el desarrollo deportivo de los atletas, con diferentes objetivos en cada una de ellas. Esto debería llevar a los entrenadores a diferentes planes de entrenamiento según la etapa de formación del atleta. El objetivo de este estudio fue analizar los planes de entrenamiento e identificar las diferencias en los objetivos de baloncesto según las etapas formativas (U'12 y U'14) en niños y niñas. Se recogieron y analizaron un total de 1.976 tareas de entrenamiento, para un total de cuatro equipos (chicas y chicos de las categorías U'12 y U'14) durante toda una temporada. Se estudiaron variables pedagógicas, fases de juego, situaciones de juego, medios de entrenamiento y contenidos. Los resultados mostraron diferencias significativas entre los géneros. Los equipos femeninos realizaron más tareas en la ofensiva y en las habilidades técnicas. Por el contrario, los equipos masculinos realizaron más tareas defensivas y contenidos tácticos. El 1 contra 0 y el 1 contra 1 fueron las situaciones de juego más repetidas en todos los equipos. Los entrenadores utilizaron diferentes tareas de entrenamiento según el género y la edad. En los equipos masculinos U'12 predominaron los ejercicios, mientras que en las otras categorías predominaron los juegos. En los equipos masculinos, los contenidos estaban orientados a la táctica, y en los equipos femeninos, a la adquisición de habilidades. El estudio de las variables pedagógicas del proceso de entrenamiento permitió identificar la utilidad del entrenamiento, la evaluación y la modificación de este procesoScientific literature has stated the presence of various stages in athletes sportive development, with different objectives in each one of them. This should lead coaches to different training plans according to the athlete’s formation stage. The aim of this study was to analyse training plans and identify differences in basketball objectives according to formative stages (U´12 and U´14) in boys and girls. A total of 1,976 training tasks were collected and analysed, for a total of four teams (girls and boys of U´12 and U´14 categories) during an entire season. Pedagogical variables, game phases, game situations, training means and content were studied. The results showed significant differences between genders. Girls’ teams performed more tasks on offense and technical skills. By contrast, boys’ teams performed more defensive tasks and tactical contents. The 1-on-0 and 1-on-1 were the most repeated game situations in all teams. Coaches used different training tasks according to gender and age. In male U´12 teams, drills predominated, whereas in the other categories, games predominated. For boys’ teams, the contents were tactical oriented, and for girls’ teams, the contents were oriented toward skill acquisition. Studying the pedagogical variables of the training process allowed for identification of the utility of training, assessment, and modification of this process.• Junta de Extremadura y Fondo Europeo para el Desarrollo Regional. Ayuda GR15122peerReviewe

Aromatic interactions of allenyl-anthracene derivatives with pi-electron acceptor molecules: an experimental and computational study

Electrostatic effects play an important role in aromatic interactions towards molecular recognition. Anthraceno-allenophanes are cyclic π-conjugated aromatic systems able to recognise complementary molecules through aromatic interactions. A computational and experimental study has shown that the dispersive contribution is the predominant stabilising term in the interaction of an allenyl-anthracene derivative with relevant π-acceptor molecules, such as pentafluorophenol, picric acid and 2,3-dichloro-5,6-dicyano-1,4-quinone.Agencia Estatal de Investigación | Ref. CTQ2017-85919-RXunta de Galicia | Ref. ED431C 2017/7Xunta de Galicia | Ref. ED431E 2018/07Xunta de Galicia | Ref. ED481B 2016/166-

Statistical study of combustion characteristics and optimal operation factor determination in an emulsion burner fuelled with vegetable oils

This work experimentally investigates the combustion characteristics of refined soya, sunflower and rapeseed vegetable oils and, by means of statistical techniques, determines the optimal operating factors of an emulsion burner to obtain the best combustion performance and low pollutant emissions. Given the high dimensionality of the study, the PCA provides a descriptive study of the variables involved in the combustion process and of the physicochemical properties of the vegetable oils so as to establish the correlations between them. ANOVA was then performed to identify which factors (type of vegetable oil, fuel flow, and airflow), as well as any possible interactions, have the greatest impact on the combustion results (performance as well as CO2, CO, NOx, CxHy and SOx emissions). ANOVA results showed that almost all of the factors and their interactions were significant, which makes it essential to analyse the interaction plots to see the optimal combinations of levels. This study showed that fuel flow rate was quite an important factor affecting combustion characteristics, that the type of vegetable oil influenced CxHy emissions, and that the airflow rate displayed no clear trend. Furthermore, the best combustion performance coupled with pollutant emissions that were below the lowest limits established by current legislation were achieved for a combination of maximum fuel flow and minimum airflow rates, with soya exhibiting the best performance. In general, good combustion performances were obtained with extremely low NOx emissions, and SOx emissions were not detected in any of the combustion experiments performed.Spanish Ministerio de Economía y Competitividad, grant MTM2017-86061-C2-1-P, and by Consejería de Educación de la Junta de Castilla y León and FEDER, grant VA005P17 and VA002G18

Statistical Study of Combustion Characteristics and Optimal Operation Factor Determination in an Emulsion Burner Fueled with Vegetable Oils

This work experimentally investigates the combustion characteristics of refined soya, sunflower, and rapeseed vegetable oils and, by means of statistical techniques, determines the optimal operating factors of an emulsion burner to obtain the best combustion performance and low pollutant emissions. Given the high dimensionality of the study, principal component analysis provides a descriptive study of the variables involved in the combustion process and of the physicochemical properties of the vegetable oils so as to establish the correlations between them. ANOVA was then performed to identify which factors (type of vegetable oil, fuel flow, and airflow), as well as any possible interactions, have the greatest impact on the combustion results (performance as well as CO2, CO, NOx, CxHy, and SOx emissions). The ANOVA results showed that almost all of the factors and their interactions were significant, which makes it essential to analyze the interaction plots in order to see the optimal combinations of levels. This study showed that fuel flow rate was quite an important factor affecting combustion characteristics, that the type of vegetable oil influenced CxHy emissions, and that the airflow rate displayed no clear trend. Furthermore, the best combustion performance coupled with pollutant emissions below the lowest limits established by the current legislation were achieved for a combination of maximum fuel flow and minimum airflow rates, with soya exhibiting the best performance. In general, good combustion performances were obtained with extremely low NOx emissions. SOx emissions were not detected in any of the combustion experiments performed.Spanish Ministerio de Economía y Competitividad, grant MTM2017-86061-C2-1-P, and by Consejería de Educación de la Junta de Castilla y León and FEDER, grant VA005P17 and VA002G18

Caracterización de un vidrio rojo medieval procedente de las vidrieras del Monasterio de las Huelgas de Burgos

En el año 2007 se culminó el proceso de restauración integral de las vidrieras que cierran los ventanales de la Sala Capitular del Claustro de S. Fernando del Real Monasterio de Santa María la Real de las Huelgas de Burgos. Un vidrio rojo, de características peculiares, extraído durante el proceso de conservación-restauración de una de las vidrieras, se ha caracterizado mediante las técnicas de Microscopía óptica de luz reflejada (MOLR), Microscopía electrónica de emisión de campo (MEEC), Fluorescencia de rayos X (FRX), Emisión de rayos X inducidos por partículas (PIXE) y Microscopía electrónica de transmisión (MET) para conocer su composición química y su estructura. Los análisis realizados por las técnicas de FRX y PIXE permiten comprobar la buena concordancia que existe entre los resultados. El vidrio analizado presenta una sección transversal muy original, formada por un vidrio soporte ligeramente verdoso y múltiples capas de color rojo, característico de los siglos XIII y XIV. El análisis mediante micro-PIXE en cámara de vacío ha permitido obtener información sobre la distribución en profundidad de los elementos que lo componen.In the year 2007 the process of conservation-restoration of the windows of the apostles that close the windows of the Chapter Hall of Cloister of S. Fernando del Real Monasterio de Santa María la Real de las Huelgas de Burgos was completed the process. A red glass obtained from the process of conservation and restoration has been characterized by Optical microscopy by light reflected (OMLR), Field emission scanning electron microscopy (FE-SEM), X ray fluorescence espectrpmetry (XRF), Particle induced X ray emission (PIXE), Transmission electron microscopy (TEM) to identify the chemical composition and structure. Chemical analys by XRF and PIXE are in the good agreement. The studied glass has a very original cross section, consisting of a lightly greenish glass holder and multilayers of red glass, characteristic of the thirteenth and fourteenth centuries. A elemental mapping of the cross-section showing the distribution of elements in the glass has been obtained by micro PIXE analysis in a vacuum chamber