Impact of Quinoline Amide Substituents on the Luminescence and PARACEST MRI properties of Bimodal Europium (III) Complexes

Lanthanide ions exhibit optical imaging properties; however, the amount of energy needed to directly excite these ions could potentially be damaging to biological tissue. To overcome this limitation, organic chromophores, such as quinolines, can be used to absorb the excited light and transfer the energy obtained to the attached lanthanide ion. This intramolecular energy transfer results in the sensitization of the lanthanide ion luminescence. The goal of this project is to assess how the amide proton position in relation to the quinoline substituent affects the sensitivity of luminescence and the optical imaging properties of Europium (III) complexes. To achieve this goal, a library of quinoline containing lanthanide complexes will be synthesized and their luminescence and PARACEST MRI properties evaluated. A quinoline substituted Europium (III) complex has been successfully synthesized and characterized by ¹H NMR and ¹³C NMR. Future plans include completion of the library of complexes as well as testing of their luminescence and PARACEST MRI characteristics

Política de educação e discurso político: The American Federation for Children redes

This article presents findings from an analysis of the AFC policy network using tools from network ethnography and qualitative content analysis. Specifically, we examined tax forms and carried out extensive web searches to spatialize and map the AFC network, mined text from policy-actors in the AFC network, and analyzed the policy discourse promoted by these network actors to achieve their political goals. The task for this study was to use AFC as a heuristic device to explore the complexity of the education policy field and to understand how network policy-actors work to achieve their policy goals through advocacy and marketing. Findings from the study indicate that the AFC network demonstrates a hierarchical ordering, this hierarchical ordering is reflective of the elite planning and social engineering associated with neoliberal reforms, and that the policy-actors in the AFC network employ discursive strategies to frame an elite political project to advance school choice policies as an anti-elite movement oriented toward political empowerment and educational justice.Este artículo presenta los resultados de un análisis de la red política American Federation for Children Network (AFC) utilizando herramientas de red de la etnografía y análisis de contenido cualitativo. En concreto, examinamos formularios de impuestos y realizó extensas investigaciones en la web para espacializar y asignar la red AFC, texto extraído de políticos-actores en la red AFC, y analizados por el discurso político Promovido Estos actores de la red para alcanzar sus objetivos políticos. La tarea para este estudio fue utilizar el AFC como un dispositivo heurístico para explorar la complejidad de la política educativa y entender cómo los actores de política de red trabajan para alcanzar sus objetivos de política a través de la defensa y el marketing. Las conclusiones del estudio indican que la red AFC demuestra una ordenación jerárquica, esta ordenación jerárquica es el reflejo de la planificación de la elite y la ingeniería social asociadas a las reformas neoliberales, y que la política-actores al servicio de la red estrategias discursivas AFC para encuadrar un proyecto político élite para hacer avanzar las políticas de elección de la escuela como un movimiento político anti-elite orientado hacia la capacitación y la justicia educativa.Este artigo apresenta os resultados de uma análise da rede política American Federation for Children Network (AFC) utilizando ferramentas de rede da etnografia e análise de conteúdo qualitativa. Especificamente, nós examinamos formulários de impostos e realizou extensas pesquisas na web para espacializar e mapear a rede AFC, texto extraído de políticos-atores na rede AFC, e analisados pelo discurso político Promovido Estes atores da rede para atingir seus objetivos políticos. A tarefa para este estudo foi usar o AFC como um dispositivo heurístico para explorar a complexidade da política educacional e entender como os atores de política de rede trabalham para alcançar seus objetivos de política por meio de advocacy e marketing. Conclusões do estudo indicam que a rede AFC Demonstra uma ordenação hierárquica, esta ordenação hierárquica é o reflexo do planejamento elite e engenharia social associados às reformas neoliberais, e que a política-atores a serviço da rede estratégias discursivas AFC para enquadrar um projeto político elite para fazer avançar as políticas de escolha da escola como um movimento político anti-elite voltada para a capacitação e justiça educacional

Potential application of cryobiopsy for histo-molecular characterization of mediastinal lymph nodes in patients with thoracic malignancies: a case presentation series and implications for future developments

Background: The management of non-small cell lung cancer (NSCLC) has become increasingly complex due to the evolution of personalized medicine approaches. Such approaches are characterized by the necessity of adequate tumor samples; hence, improved biopsy techniques are needed. Transbronchial lung cryobiopsy is a novel endoscopic procedure designed to collect peripheral pulmonary tissue, and it is currently employed in interstitial lung diseases. The use of this technique in oncology might result in improved mediastinum staging and molecular characterizations; however, available data involving the use of a cryoprobe on mediastinal lymph nodes are still limited. Case presentation: Here we present a series of five consecutive patients who underwent endoscopic assessment of mediastinal lymph nodes for oncologic reasons. All patients were subjected both to endobronchial ultrasound-guided transbronchial needle aspiration (EBUS TBNA) and cryobiopsy of mediastinal lymph nodes during the same procedure, and no complications were observed. In three of the reported cases, both cryobiopsy and cell block from EBUS TBNA were positive, while in one case cryobiopsy was not diagnostic and EBUS TBNA was negative; moreover, one case showed discordance between the procedures, as cryobiopsy was negative and cell block obtained from multiple stations was diagnostic for small cell lung cancer. In one case involving a patient treated for lymphoma, cryobiopsy provided more complete histologic characterization, and in another case involving a patient affected by NSCLC cryobiopsy provided more material for molecular analyses. Conclusion: This case presentation series suggests that cryobiopsy, which has been generally used on peripheral lung lesions so far, is a feasible and safe approach for diagnosis and staging of mediastinal lymph nodal involvement, especially when station 7 is involved. Compared to EBUS TBNA, cryobiopsy might provide more adequate histological samples, with a possible impact on molecular characterizations and, therefore, therapeutic decisions. However, the learning curve of the procedure has not to be understated and optimal protocols for implementing this technique are needed. In our opinion, further studies designed to integrate the routine use of cryobiopsy in current practice for solid and eventually hematologic tumors with mediastinal lymph node involvement are warranted

Quasi-experimental study designs series –Paper 9: Collecting Data from Quasi-Experimental Studies

Objective: To identify variables that must be coded when synthesizing primary studies that use quasi-experimental designs.  Study Design and Setting: All quasi-experimental (QE) designs.  Results: When designing a systematic review of QE studies potential sources of heterogeneity – both theory-based and methodological – must be identified. We outline key components of inclusion criteria for syntheses of quasi-experimental studies. We provide recommendations for coding content-relevant and methodological variables, and outlined the distinction between bivariate effect sizes and partial (i.e., adjusted) effect sizes. Designs used and controls employed are viewed as of greatest importance. Potential sources of bias and confounding are also addressed.  Conclusion: Careful consideration must be given to inclusion criteria and the coding of theoretical and methodological variables during the design phase of a synthesis of quasi-experimental studies. The success of the meta-regression analysis relies on the data available to the meta-analyst. Omission of critical moderator variables (i.e., effect modifiers) will undermine the conclusions of a meta-analysis

Quasi-experimental study designs series – Paper 10: Synthesizing evidence for effects collected from quasi-experimental studies presents surmountable challenges

Objective: To outline issues of importance to analytic approaches to the synthesis of quasi-experiments (QEs), and to provide a statistical model for use in analysis. Study Design and Setting: We drew on the literatures of statistics, epidemiology, and social-science methodology to outline methods for synthesis of QE studies. The design and conduct of quasi-experiments, effect sizes from QEs, and moderator variables for the analysis of those effect sizes were discussed. Results: Biases, confounding, design complexities and comparisons across designs offer serious challenges to syntheses of QEs. Key components of meta-analyses of QEs were identified, including the aspects of QE study design to be coded and analyzed. Of utmost importance are the design and statistical controls implemented in the QEs. Such controls and any potential sources of bias and confounding must be modeled in analyses, along with aspects of the interventions and populations studied. Because of such controls, effect sizes from QEs are more complex than those from randomized experiments. A statistical meta-regression model that incorporates important features of the QEs under review was presented. Conclusion: Meta-analyses of quasi-experiments provide particular challenges, but thorough coding of intervention characteristics and study methods, along with careful analysis, should allow for sound inferences

A multidisciplinary expert opinion on CINV and RINV, unmet needs and practical real-life approaches

Introduction: A range of combination chemotherapy regimens are currently used in clinical practice. However, international antiemetic guidelines often only categorize the emetogenic potential of single agents rather than the emetogenicity of combination chemotherapy regimens. To manage the nausea and vomiting induced by antineoplastic combinations, guidelines suggest antiemetics that are appropriate for the component drug with the highest emetogenic potential. Furthermore, antiemetic guidelines generally do not consider the influence of other factors, including individual patient characteristics, on the emetic effects of cancer treatments. Similarly, the emetogenic potential of radiotherapy is stratified only according to the site of radiation, while other factors contributing to emetic risk are overlooked. Areas covered: An Expert Panel was convened to examine unresolved issues and summarize the current clinical research on managing nausea and vomiting associated with combination chemotherapy and radiotherapy. Expert opinion: The panel identified the incidence of nausea and vomiting induced by multi-drug combination therapies currently used to treat cancer at different anatomic sites and by radiotherapy in the presence of other risk factors. Based on these data and the clinical experience of panel members, several suggestions are made for a practical approach to prevent or manage nausea and vomiting due to chemotherapy regimens and radiation therapy

CD4+CD25+ lymphocyte subsets in chronic graft versus host disease patients undergoing extracorporeal photochemotherapy.

Extracorporeal photochemotherapy (ECP) has been used successfully for the treatment of chronic Graft versus Host Disease (cGvHD). However, the mechanism by which ECP exerts its protective effects remains elusive. Some recent observations have suggested a possible role of certain subsets of T lymphocytes with immunosuppressive properties (T-regulatory cells) that coexpress CD4 and high levels of the interleukin-2 receptor chain: CD4+CD25+ T lymphocytes. We studied whether ECP affects the percentage of these cells in the peripheral blood of patients with cGvHD. The study population consisted of 14 patients with cGvHD refractory to systemic steroids. On enrolment in each cycle of ECP, patients underwent clinical examination, blood chemistry analysis and other instrumental procedures to document and assess involvement of the various organs and systems. For cytofluorimetric identification and phenotyping of CD4+CD25+ T lymphocytes, peripheral blood samples were collected in EDTA anticoagulant before ECP, after 48 hours, and after 6 and 12 months from the start of treatment. The 14 patients in this study received a total of more than 300 cycles of ECP, with only minor side effects. The clinical outcome was negative in 2 patients and positive in 12 patients. Within-subject analysis indicated that the percentage of CD4+CD25+ T lymphocytes before ECP and after 12 months of treatment was significantly increased. Our study confirms that changes in the percentage of CD4+CD25+ T cells induced by ECP could be a central aspect in the cascade of immune events leading to the immunological and clinical effects of this treatment in patients with cGvHD

Quasi-experimental study designs series-paper 6: risk of bias assessment.

OBJECTIVES: Rigorous and transparent bias assessment is a core component of high-quality systematic reviews. We assess modifications to existing risk of bias approaches to incorporate rigorous quasi-experimental approaches with selection on unobservables. These are nonrandomized studies using design-based approaches to control for unobservable sources of confounding such as difference studies, instrumental variables, interrupted time series, natural experiments, and regression-discontinuity designs. STUDY DESIGN AND SETTING: We review existing risk of bias tools. Drawing on these tools, we present domains of bias and suggest directions for evaluation questions. RESULTS: The review suggests that existing risk of bias tools provide, to different degrees, incomplete transparent criteria to assess the validity of these designs. The paper then presents an approach to evaluating the internal validity of quasi-experiments with selection on unobservables. CONCLUSION: We conclude that tools for nonrandomized studies of interventions need to be further developed to incorporate evaluation questions for quasi-experiments with selection on unobservables

Nerve growth factor is an autocrine survival factor for memory B lymphocytes

AbstractProduction of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD “virgin” B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific IgG in mice immunized with tetanus toxoid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes