Expression and Localization of aquaporin‐1 in Temporomandibular Joint Disc with Internal Derangement

Background: Internal derangement is the most frequent arthropathy affecting the temporomandibular joint, where its commonest form is anterior disc displacement with or without reduction. Despite the frequency of the disorder, the biochemical features of displaced discs are still unclear. Methods: We investigated the expression pattern and localization of aquaporin‐1, an important channel protein involved in plasma membrane water permeability, in patients with anterior disc displacement (both with and without reduction), with a view to assessing the characteristics of local tissue responses to the microenvironmental changes induced by abnormal mechanical loading of the displaced disc. Protein expression was studied by immunohistochemistry in different areas of discs from 18 patients with anterior disc displacement with or without reduction and in four normal controls. Results: A greater proportion of cells immunopositive for aquaporin‐1 were detected in diseased than in normal discs. Whereas protein expression was substantially similar in the different areas of normal discs, a significantly larger number of immunopositive cells were detected in the posterior band of displaced discs without reduction and in the anterior and intermediate bands of those with reduction. Conclusions: These findings suggest that aquaporin‐1 is expressed and upregulated in temporomandibular joint with anterior disc displacement (both with and without reduction)

Interaction with human plasminogen system turns on proteolytic activity in Streptococcus agalactiae and enhances its virulence in a mouse model

Interactions of several microbial pathogens with the plasminogen system increase their invasive potential. In this study, we show that Streptococcus agalactiae binds human plasminogen which can be subsequently activated to plasmin, thus generating a proteolytic bacterium. S. agalactiae binds plasminogen via the direct pathway, using plasminogen receptors, and via the indirect pathway through fibrinogen receptors. The glyceraldehyde-3-phosphate dehydrogenase is one If the S. agalactiae proteins that bind plasminogen. Presence of exogenous activators such as uPA and tPA are required to activate bound plasminogen. Results from competitive inhibition assays indicate that binding is partially mediated through the lysine binding sites of plasminogen. Following plasminogen binding and activation, S. agalactiae is able to degrade in vitro fibronectin, one of the host extracellular matrix proteins. Moreover, incubation of S. agalactiae with either plasminogen alone, or plasminogen plus fibrinogen, in the presence of tPA enhanced its virulence in C57BL/6 mice, suggesting that acquisition of plasmin-like activity by the bacteria increase their invasiveness

Interaction with human plasminogen system turns on proteolytic activity in Streptococcus agalactiae and enhances its virulence in a mouse model

Interactions of several microbial pathogens with the plasminogen system increase their invasive potential. In this study, we show that Streptococcus agalactiae binds human plasminogen which can be subsequently activated to plasmin, thus generating a proteolytic bacterium. S. agalactiae binds plasminogen via the direct pathway, using plasminogen receptors, and via the indirect pathway through fibrinogen receptors. The glyceraldehyde-3-phosphate dehydrogenase is one If the S. agalactiae proteins that bind plasminogen. Presence of exogenous activators such as uPA and tPA are required to activate bound plasminogen. Results from competitive inhibition assays indicate that binding is partially mediated through the lysine binding sites of plasminogen. Following plasminogen binding and activation, S. agalactiae is able to degrade in vitro fibronectin, one of the host extracellular matrix proteins. Moreover, incubation of S. agalactiae with either plasminogen alone, or plasminogen plus fibrinogen, in the presence of tPA enhanced its virulence in C57BL/6 mice, suggesting that acquisition of plasmin-like activity by the bacteria increase their invasiveness

REPRODUCTIVE PLANNING AND INSERTION OF INTRAUTERINE DEVICES BY PHYSICIANS AND NURSES IN BRAZIL

Objective: to analyze the records referring to reproductive planning consultations and to intrauterine device insertion performed in 2021 by nurses and physicians in Primary Health Care in Brazil.Method: this is a quantitative, cross-sectional and descriptive study that resorted to secondary data from the Health Information System for Primary Care and submitted to simple descriptive statistics data analysis.Results: a total of 18,243 procedures about IUD insertion were recorded in the country, with prevalence of physicians, except for the state of Roraima; as well as 54,186 reproductive planning consultations with predominance of 41,184 (76%) nurses in relation to physicians (13,002; 24%).Conclusion: there is a need to invest in the training of physicians and nurses in order to expand access and the right to sexual life care of the women living in the country. Nursing care is a way to consolidate women's right to sexual and reproductive life care

PROCESSO SUCESSÓRIO EM EMPRESAS FAMILIARES:: uma leitura comunicacional, a partir do pensamento sistêmico

O artigo teórico, exploratório, qualitativo, interpretao processo sucessório em empresa familiar a partirdo arcabouço teórico do Pensamento Sistêmico, emNiklas Luhmann, que tem como viés a construção darealidade com base na interação e na comunicação.O processo sucessório familiar é um tema de amplainvestigação na Administração, mas em Comunicaçãoainda é pouco estudado. A empresa familiar possuicontratos reificados na Cultura Organizacional quesão colocados em cheque em processos de trocade lideranças, fusões, transição de forma jurídica(responsabilidade limitada, sociedade anônimas,capital aberto) e profissionalização da gestão.O planejamento para a sucessão de geraçõescostuma ter desafios a serem enfrentados porempresas familiares locais, regionais, nacionais emultinacionais. No artigo, analisamos o fenômenoa partir de variáveis como nepotismo, paternalismo,protecionismo, comodismo, relacionamento compúblicos, circulação de informação organizacional,presença (ou não) de planejamento, identificadas emRosário (2016). Utilizamos a baliza da ComunicaçãoOrganizacional como fenômeno, como processo ecomo sistema (CURVELLO, 2009). O objetivo é o deofertar uma interpretação da comunicação e dasorganizações como sistemas complexos: uma leituracomunicacional da estratégia (GOMES, 2014) nessesprocessos sucessórios

PLANEJAMENTO REPRODUTIVO E INSERÇÃO DE DISPOSITIVO INTRAUTERINO REALIZADA POR MÉDICOS E ENFERMEIRAS NO BRASIL

Objetivo: analisar os registros referentes à consulta de planejamento reprodutivo e a inserção do dispositivo intrauterino realizadas por enfermeiros e médicos na Atenção Primária à Saúde do Brasil, no ano de 2021.Método: trata-se de estudo quantitativo, transversal e descritivo, utilizando-se de dados secundários do Sistema de Informação em Saúde para a Atenção Básica e submetido à análise estatística descritiva simples dos dados.Resultados: foram registrados no país 18.243 procedimentos sobre a inserção do DIU com prevalência da atuação do profissional médico, exceto no estado de Roraima; e o quantitativo de 54.186 consultas de planejamento reprodutivo com hegemonia de 41.184 (76%) do profissional enfermeiro em relação aos médicos 13.002 (24%).Conclusão: há necessidade de investimento na capacitação de médicos e enfermeiros para ampliar o acesso e direito à atenção à vida sexual das mulheres no país. A atuação da Enfermagem se constitui em uma via para consolidar o direito à atenção à vida sexual e reprodutiva das mulheres

PLANIFICACIÓN REPRODUCTIVA E INSERCIÓN DE DISPOSITIVOS INTRAUTERINOS REALIZADAS POR MÉDICOS Y ENFERMERAS EN BRASIL

Objetivo: analizar los registros sobre la consulta de planificación reproductiva y la inserción del dispositivo intrauterino realizadas por enfermeros y médicos en la Atención Primaria de Salud en Brasil, en el año 2021.Método: se trata de un estudio cuantitativo, transversal y descriptivo, utilizando datos secundarios del Sistema de Información en Salud para la Atención Primaria y sometidos a análisis estadístico descriptivo simple de los datos.Resultados: se registraron 18.243 procedimientos de inserción de DIU en el país, en los que predominaron los profesionales médicos, excepto en el estado de Roraima; mientras que, de las 54.186 consultas de planificación reproductiva registradas, 41.184 (76%) fueron realizadas por los profesionales de enfermería y 13.002 (24%) por médicos.Conclusión: es necesario invertir en la formación de médicos y enfermeros para ampliar el acceso y el derecho al cuidado de la vida sexual de las mujeres en el país. El trabajo de Enfermería constituye una forma de consolidar el derecho al cuidado de la vida sexual y reproductiva de las mujeres

Interlaboratory Coverage Test on Plant Food Bioactive Compounds and their Metabolites by Mass Spectrometry-Based Untargeted Metabolomics.

Bioactive compounds present in plant-based foods, and their metabolites derived from gut microbiota and endogenous metabolism, represent thousands of chemical structures of potential interest for human nutrition and health. State-of-the-art analytical methodologies, including untargeted metabolomics based on high-resolution mass spectrometry, are required for the profiling of these compounds in complex matrices, including plant food materials and biofluids. The aim of this project was to compare the analytical coverage of untargeted metabolomics methods independently developed and employed in various European platforms. In total, 56 chemical standards representing the most common classes of bioactive compounds spread over a wide chemical space were selected and analyzed by the participating platforms (n = 13) using their preferred untargeted method. The results were used to define analytical criteria for a successful analysis of plant food bioactives. Furthermore, they will serve as a basis for an optimized consensus method

Conceptual framework for the definition of preclinical and prodromal frontotemporal dementia

The presymptomatic stages of frontotemporal dementia (FTD) are still poorly defined and encompass a long accrual of progressive biological (preclinical) and then clinical (prodromal) changes, antedating the onset of dementia. The heterogeneity of clinical presentations and the different neuropathological phenotypes have prevented a prior clear description of either preclinical or prodromal FTD. Recent advances in therapeutic approaches, at least in monogenic disease, demand a proper definition of these predementia stages. It has become clear that a consensus lexicon is needed to comprehensively describe the stages that anticipate dementia. The goal of the present work is to review existing literature on the preclinical and prodromal phases of FTD, providing recommendations to address the unmet questions, therefore laying out a strategy for operationalizing and better characterizing these presymptomatic disease stages