Ponderação econômica para suporte de sistema de melhoramento genético de bovinos de corte criados extensivamente na sub-região da Nhecolândia, Pantanal Sul-Mato-Grossense. Análise de custos e receitas.

Objetivou-se com este trabalho avaliar e definir a importância econômica de possíveis características produtivas e reprodutivas a serem utilizadas como suporte na seleção de bovinos de corte em função de seus valores econômicos. Com base em informações de desempenho de índices técnicos e econômicos foi desenvolvido um cenário produtivo de uma propriedade modal que realiza o sistema extensivo de produção de bovinos de corte (fase de cria) na sub - região da Nhecolândia, Pantanal Sul - Mato - Grossense. Foi desenvolvido um modelo determinístico por meio de planilhas eletrônicas para a realização dos cálculos de custos e de receitas. O lucro foi estimado pela diferença entre os custos e receitas do sistema de produção estudado. As características números de bezerros desmamados e número de vacas de descarte foram as que apresentaram maior remuneração para o sistema de produção avaliado

PALM and STORM: Into large fields and high-throughput microscopy with sCMOS detectors

Single Molecule Localization Microscopy (SMLM) techniques such as Photo-Activation Localization Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM) enable fluorescence microscopy super-resolution: the overcoming of the resolution barrier imposed by the diffraction of light. These techniques are based on acquiring hundreds or thousands of images of single molecules, locating them and reconstructing a higher-resolution image from the high-precision localizations. These methods generally imply a considerable trade-off between imaging speed and resolution, limiting their applicability to high-throughput workflows. Recent advancements in scientific Complementary Metal-Oxide Semiconductor (sCMOS) camera sensors and localization algorithms reduce the temporal requirements for SMLM, pushing it toward high-throughput microscopy. Here we outline the decisions researchers face when considering how to adapt hardware on a new system for sCMOS sensors with high-throughput in mind

Lusitanian toadfish song reflects male quality

Lusitanian toadfish males that provide parental care rely on acoustic signals (the boatwhistle) to attract females to their nest. We test the hypothesis that male quality, namely male size and condition that are relevant for parental success, is reflected in vocal activity and boatwhistle characteristics and thus advertised to females. We recorded 22 males over a week during the peak of the breeding season. Calling rate and calling effort (percentage of time spent calling) strongly reflected male condition (lipid content of somatic muscles) and to a smaller extent sonic muscle hypertrophy and larger gonads. Males in better condition (increased body lipid and relative higher liver mass) also contracted the sonic muscles at faster rate as shown by the shorter boatwhistle pulse periods. Amplitude modulation reflected the degree of sonic muscle hypertrophy. None of the measured male quality parameters were good predictors of boatwhistle duration and dominant frequency. Altogether this study strongly suggests that Lusitanian toadfish males advertise their quality to females primarily with boatwhistle calling rate and calling effort, which mainly reflect male condition. Because pulse period had low variability, consistent with the existence of a vocal central pattern generator, we suggest that males that sustain sonic muscles contraction at a very fast rate close to their physiological limit may be honestly advertising their quality (condition). Similarly, males that produce boatwhistles with higher amplitude modulation, a feature that seems dependent on sonic muscle hypertrophy, could be more attractive to females.Fundação para a Ciência e Tecnologiainfo:eu-repo/semantics/publishedVersio

Planar Airy beam light-sheet for two-photon microscopy

We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.Comment: 7 pages, 4 figure