13 research outputs found
Special and Inclusive Teachers’ Experience in The Distance Learning
This study was designed to explore the lived experiences of special and inclusive teachers in distance learning and gained necessary perspective about their different experiences and challenges in the new normal education in distance learning. It utilized the use of phenomenological research design and simple random sampling on choosing the participants. Data were analyzed through thematic analysis method. The results indicated that the teachers felt inadequately prepared to teach atypical learners in distance learning because of some factors, examples are lack of resources of teachers and students. Almost all participants indicated that they discovered their own teaching strategies in handling atypical learners. In addition, the teachers make use of the available technology by utilizing different educational applications. Also, the majority of the teachers motivate their students to continue to learn in the new normal. Implications of these results are discussed
A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction
MLKL is the essential effector of necroptosis, a form of programmed lytic cell death. We have isolated a mouse strain with a single missense mutation, Mlkl(D139V), that alters the two-helix 'brace' that connects the killer four-helix bundle and regulatory pseudokinase domains. This confers constitutive, RIPK3 independent killing activity to MLKL. Homozygous mutant mice develop lethal postnatal inflammation of the salivary glands and mediastinum. The normal embryonic development of Mlkl(D139V) homozygotes until birth, and the absence of any overt phenotype in heterozygotes provides important in vivo precedent for the capacity of cells to clear activated MLKL. These observations offer an important insight into the potential disease-modulating roles of three common human MLKL polymorphisms that encode amino acid substitutions within or adjacent to the brace region. Compound heterozygosity of these variants is found at up to 12-fold the expected frequency in patients that suffer from a pediatric autoinflammatory disease, chronic recurrent multifocal osteomyelitis (CRMO). Necroptosis is a regulated form of inflammatory cell death driven by activated MLKL. Here, the authors identify a mutation in the brace region that confers constitutive activation, leading to lethal inflammation in homozygous mutant mice and providing insight into human mutations in this region
Utilization of Next Generation Sequencing Identifies Potentially Actionable Mutations with Prognostic Significance in Chronic Lymphocytic Leukemia
A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction
MLKL is the essential effector of necroptosis, a form of programmed lytic cell death. We have isolated a mouse strain with a single missense mutation, Mlkl, that alters the two-helix ‘brace’ that connects the killer four-helix bundle and regulatory pseudokinase domains. This confers constitutive, RIPK3 independent killing activity to MLKL. Homozygous mutant mice develop lethal postnatal inflammation of the salivary glands and mediastinum. The normal embryonic development of Mlkl homozygotes until birth, and the absence of any overt phenotype in heterozygotes provides important in vivo precedent for the capacity of cells to clear activated MLKL. These observations offer an important insight into the potential disease-modulating roles of three common human MLKL polymorphisms that encode amino acid substitutions within or adjacent to the brace region. Compound heterozygosity of these variants is found at up to 12-fold the expected frequency in patients that suffer from a pediatric autoinflammatory disease, chronic recurrent multifocal osteomyelitis (CRMO)
Comparable outcomes in CLL patients treated with reduced-dose ibrutinib: Results from a multi-center study.
Favorable Outcomes in CLL Pts with Alternate Kinase Inhibitors Following Ibrutinib or Idelalisib Discontinuation: Results from a Large Multi-Center Study
Differentiation and Identification of Cachac̀§a Wood Extracts Using Peptide-Based Receptors and Multivariate Data Analysis
It is becoming increasingly
important to differentiate complex
mixtures, especially in forensics. Cachac̀§a, the most popular
alcoholic beverage in Brazil, is made from distilled and fermented
sugar cane juice. It contains a mixture of naturally occurring polyphenols
known as tannins, whose composition is dictated by the type of wood
used to age the beverage. These tannins can be differentiated in an
Indicator Displacement Assay (IDA) using peptide-based ternary sensing
ensembles. This investigation demonstrates a technique for fingerprinting
the identity of the woods used to age cachac̀§as. Unknown cachac̀§a
samples were tested against a training set of Brazilian woods in addition
to oaks from different countries. Results obtained from the analysis
showed that 62.5% of the samples were correctly identified. Furthermore,
four samples anonymously added to the pool of unknowns from the training
set were identified with 100% accuracy, emphasizing both the promising
results obtained from this method of differentiation and the importance
of analyzing same-age samples
A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction
Necroptosis is a regulated form of inflammatory cell death driven by activated MLKL. Here, the authors identify a mutation in the brace region that confers constitutive activation, leading to lethal inflammation in homozygous mutant mice and providing insight into human mutations in this region