Concert recording 2017-11-06

[Track 1]. Sebben crudele / Antonio Caldara -- [Track 2]. Orpheus with his lute / William Schuman -- [Track 3]. Heaven help my heart -- [Track 4]. Little elegy / John Duke -- [Track 5]. Bel piacere / G.F. Handel -- [Track 6]. I dreamed a dream from Les Miserables / Claude-Michel Schönberg -- [Track 7]. In questa tomba oscura / Ludwig van Beethoven -- [Track 8]. Lasst mich nur auf meinem Sattel gelten! from Freisinn / Robert Shumann -- [Track 9]. Für Musik / Robert Franz -- [Track 10]. Edelweiss from The sound of music / Rodgers & Hammerstein -- [Track 11]. Tu lo sai / Giuseppe Torelli -- [Track 12]. Glück / Max Reger -- [Track 13] Le colibri / Ernest Chausson -- [Track 14] Der Der Schäfer / Hugo Wolf -- [Track 15]. A route to the sky / Jake Heggie -- [Track 16]. Ich liebe dich / Edvard Grieg -- [Track 17]. When it all fall down from Chaplin / Christopher Curtis -- [Track 18]. Voyage a Paris / Francis Poulenc -- [Track 19]. Crucifixion / arr. Hall Johnson -- [Track 20]. Bright is the ring of words / Ralph Vaughan Williams -- [Track 21]. Non t\u27amo piu / Francesco Paolo Tosti -- [Track 22]. Se tu m\u27ami / Alessandro Parisotti -- [Track 23]. I love the way from Something\u27s rotten / Kirkpatrick & Kirkpatrick -- [Track 24]. A madrigal / Herbert Howells -- [Track 25]. El majo discrete / Enrique Granados -- [Track 26]. The roadside fire / Ralph Vaughan Williams -- [Track 27]. Che fiero costume / Giovanni Legrenzi -- [Track 28]. An die Musik / Franz Shubert -- [Track 29]. I will sing new songs of gladness / Anton Dvorak -- [Track 30]. Full fathom five from The tempest / Henry Purcell -- [Track 31]. Nimmerstate Libe / Hugo Wolf -- [Track 32]. Deposuit potentes from Magnificat / Johann Sebastian Bach -- [Track 33]. Vaghissima sembiante / Stefano Donaudy -- [Track 34]. Tormani a vaghegghiar from Alcina / G.F. Handel -- [Track 35]. Nobody knows this little rose / John Duke -- [Track 36]. Let beauty awake / Ralph Vaughan Williams -- [Track 37]. Steal away / arr. Hall Johnson -- [Track 38]. Come paride vezzoso from L\u27elisir d\u27amore / Kirkpatrick & Kirkpatrick -- [Track 39]. Dich, teure Halle from Tannhaüser / Richard Wagner -- [Track 40]. Non t\u27accotare all\u27urna / Giuseppe Verdi -- [Track 41]. Zur Rosenzeit / Edvard Grieg

B-RAF Mutant Alleles Associated with Langerhans Cell Histiocytosis, a Granulomatous Pediatric Disease

Langerhans cell histiocytosis (LCH) features inflammatory granuloma characterised by the presence of CD1a+ dendritic cells or 'LCH cells'. Badalian-Very et al. recently reported the presence of a canonical (V600E)B-RAF mutation in 57% of paraffin-embedded biopsies from LCH granuloma. Here we confirm their findings and report the identification of two novel B-RAF mutations detected in LCH patients.Mutations of B-RAF were observed in granuloma samples from 11 out of 16 patients using 'next generation' pyrosequencing. In 9 cases the mutation identified was (V600E)B-RAF. In 2 cases novel polymorphisms were identified. A somatic (600DLAT)B-RAF insertion mimicked the structural and functional consequences of the (V600E)B-RAF mutant. It destabilized the inactive conformation of the B-RAF kinase and resulted in increased ERK activation in 293 T cells. The (600DLAT)B-RAF and (V600E)B-RAF mutations were found enriched in DNA and mRNA from the CD1a+ fraction of granuloma. They were absent from the blood and monocytes of 58 LCH patients, with a lower threshold of sequencing sensitivity of 1%-2% relative mutation abundance. A novel germ line (T599A)B-RAF mutant allele was detected in one patient, at a relative mutation abundance close to 50% in the LCH granuloma, blood monocytes and lymphocytes. However, (T599A)B-RAF did not destabilize the inactive conformation of the B-RAF kinase, and did not induce increased ERK phosphorylation or C-RAF transactivation.Our data confirmed presence of the (V600E)B-RAF mutation in LCH granuloma of some patients, and identify two novel B-RAF mutations. They indicate that (V600E)B-RAF and (600DLAT)B-RAF mutations are somatic mutants enriched in LCH CD1a(+) cells and absent from the patient blood. Further studies are needed to assess the functional consequences of the germ-line (T599A)B-RAF allele

Institutional and Individual Factors Affecting Scientists\u27 Data-Sharing Behaviors: A Multilevel Analysis

The objective of this research was to investigate the institutional and individual factors that influence scientists\u27 data-sharing behaviors across different scientific disciplines. Two theoretical perspectives, institutional theory, and theory of planned behavior, were employed in developing a research model that showed the complementary nature of the institutional and individual factors influencing scientists\u27 data-sharing behaviors. This research used a survey method to examine to what extent those institutional and individual factors influence scientists\u27 data-sharing behaviors in a range of scientific disciplines. A national survey (with 1,317 scientists in 43 disciplines) showed that regulative pressure by journals, normative pressure at a discipline level, and perceived career benefit and scholarly altruism at an individual level had significant positive relationships with data-sharing behaviors, and that perceived effort had a significant negative relationship. Regulative pressure by funding agencies and the availability of data repositories at a discipline level and perceived career risk at an individual level were not found to have any significant relationships with data-sharing behaviors