Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico

The representation of the gene content of an organism is impacted by several factors, ranging from sampling to sequencing and then the genome assembly task. The genome assembly process can generate errors that are related to insufficient coverage in the data set, an inadequate assembly methodology, and finally, errors related to the limitation of the assembly software used. Thus, some genes remain unidentified both in complete and draft genomes, this incomplete gene knowledge impacts on several organisms, mainly of medical and industrial interest, such as Bifidobacterium breve, a Gram-positive bacterium, found in the gastrointestinal microbiota of mammals, including humans, and has beneficial probiotic activities. Therefore, the objective of this work is to identify the new gene products not represented in the genome of Bifidobacterium breve DS15-17 using the raw reads of this organism. The reads were produced from the sequencing with the Illumina MiSeq platform. PAN2HGENE software was used to identify new gene products. After the analysis, 44 new gene products were identified, 26 with described function and 18 hypothetical proteins. The hypothetical proteins identified were analyzed in the ProtoNet and Superfamily databases

The Impact of Sex and Performance Level on Pacing Behavior in a 24-h Ultramarathon

Purpose: We analyzed the impact of sex, performance level and substantial speed reductions (SSR) on pacing in the VI Rio 24-h Marines Ultramarathon. This will provide insights into the importance of minimizing speed variations in relation to optimal pacing in endurance events. Methods: Runners (30 males and 21 females), classified as high- (HP) and low-performance (LP) ran the race while having their time recorded every 400 m. The pacing was analyzed as the first 10% (initial epoch), the following 80% (intermediate epoch) and the last 10% of the race (final epoch). The time percentage spent at speeds 8.0 km·h−1 (running speed) was calculated. Results: Runners showed a reverse J-shaped pacing (P < 0.001) regardless of sex and performance level, although male (P < 0.004) and HP runners (P < 0.001) have preserved a higher mean speed throughout the race. Male and HP runners spent more time at running speed (P < 0.001) and less time at SSR (P < 0.001) than female and LP runners. Total distance was inversely correlated with the number of SSR and speed CV in male (r = −0.47 and r = −0.64, respectively) and female (r = −0.61 and r = −0.47, respectively). Conclusion: Male, HP runners showed less SSR, conserving a higher mean speed with less variation throughout the race. Results suggest that conservative pacing strategies, with lower speeds in the beginning and higher speeds toward the end, may be the most adequate for different endurance running disciplines. Results also show different competition dynamics between men and women, which warrants further exploration in ultramarathons as well as other IAAF events

Ocurrence of Nyssomyia intermedia (Lutz & Neiva) (Diptera: Psychodidae) in Paraná State, south of Brazil

Nyssomyia intermedia s. lat. tem sido citada por vários autores no Paraná. No entanto, alguns estudos apontam que esse táxon corresponde a Nyssomyia neivai (Pinto). Em coletas realizadas em galinheiro e em ambiente de mata, com armadilhas, entre novembro de 2005 e outubro de 2006, em Adrianópolis, Morretes e Pontal do Paraná, localizados na região de Mata Atlântica na Serra do Mar e no litoral do Paraná, sete fêmeas de Nyssomyia intermedia s. str. (Lutz & Neiva) foram encontradas juntamente com outras 14 espécies de flo ebotomíneos, confirmando a ocorrência de N.intermedia em área de costa e de mata Atlântica do Paraná.The occurrence of Nyssomyia intermedia s.lat. in the state of Paraná, Brazil, has been registered by several authors; however, studies have identified this taxon as belonging, in Paraná, to Nyssomyia neivai (Pinto). During captures with traps in a hen-house and forested areas, from November 2005 to October 2006, in Adrianópolis, Morretes and Pontal do Paraná, situated in the Atlantic forest domain, Paraná state, seven females of Nyssomyia intermedia s.str. (Lutz & Neiva) were collected together with other 14 sand floy species. Thus the occurrence of N. intermedia on the coast and in areas of Atlantic forest in Paraná is confirmed

CONHECIMENTOS, ATITUDES E PRÁTICA DE HOMENS SOBRE INFECÇÕES SEXUALMENTE TRANSMISSÍVEIS

Objectives: to analyze men’s knowledge, attitudes and practices regarding sexually transmitted infections and condom use. Method: a descriptive, transversal study with a quantitative approach, with truck drivers in the city of Itabaiana, in the Brazilian State of Sergipe. Data collection took place between January and March 2017, with 75 truck drivers. After data collection, the interviews were transcribed, analyzed and classified in terms of the truck drivers’ knowledge, attitudes and practice. Results: of the truck drivers, 74 (98.6%) possessed adequate knowledge regarding the infections; however, it was possible to identify that their attitudes and practices were not adequate – mainly in regard to sexual behavior and previous infection with sexually transmitted infections. Conclusion: effective interventions, supported by situational strategic planning, must be encouraged for the population studied in different regions of Brazil. This would reduce chains of transmission of the diseases and promote health.Objetivos: analizar conocimientos, actitudes y prácticas de hombres acerca de las infecciones sexualmente transmisibles y el uso del preservativo. Método: estudio descriptivo, transversal de abordaje cuantitativo con camioneros del municipio de Itabaiana, Sergipe. La obtención de datos ocurrió entre enero y marzo de 2017 con 75 camioneros. Después de la obtención de datos, se transcribieron las entrevistas, y fueron analizadas y clasificadas por el conocimiento, actitud y práctica de los camioneros. Resultados: de los camioneros, 74 (98,6%) presentaban conocimiento apropiado acerca de las infecciones, sin embargo fue posible identificar que las actitudes y las prácticas no eran adecuadas, principalmente con relación al comportamiento sexual y a los antecedentes de infecciones sexualmente transmisibles. Conclusión: intervenciones efectivas, subsidiadas por planeamiento estratégico situacional deben ser estimuladas para la población estudiada en diferentes regiones brasileñas. Así, disminuyen las cadenas de transmisión de enfermedades y se promueve salud.Objetivos: analisar conhecimentos, atitudes e práticas de homens acerca das infecções sexualmente transmissíveis e o uso do preservativo. Método: estudo descritivo, transversal com abordagem quantitativa com caminhoneiros do município de Itabaiana, Sergipe. A coleta de dados ocorreu entre janeiro e março de 2017 com 75 caminhoneiros. Após a coleta de dados, as entrevistas foram transcritas, analisadas e classificadas pelo conhecimento, atitude e prática dos caminhoneiros. Resultados: dos caminhoneiros, 74 (98,6%) possuíam conhecimento adequado acerca das infecções, porém foi possível identificar que as atitudes e as práticas não se apresentavam de maneira adequada, principalmente no que diz respeito ao comportamento sexual e os antecedentes de infecções sexualmente transmissíveis. Conclusão: intervenções efetivas, subsidiadas por planejamento estratégico situacional, devem ser estimuladas para a população estudada em diferentes regiões brasileiras. Assim, reduzem cadeias de transmissão das doenças e promovem saúde

Study of green area index and vegetation cover of squares in the Gurupí city, TO.

O presente trabalho objetivou estudar as praças públicas de Gurupi por meio dos cálculos dos índices de áreas verdes e cobertura vegetal. Com auxílio da trena obteve-se as medidas das praças e da projeção das copas das árvores. Através do programa Google Earth determinou-se as áreas dos setores e do perímetro urbano. Com as medidas, fez-se a classificação das praças quanto a Parque de Vizinhança e Parque de Bairro. Posteriormente realizaram-se os cálculos dos Índices de: Área Verde Total (IAVT), Área Verde para Parques de Vizinhança e Parques de Bairro (IAVPV e IAVPB), Área Verde por Setor (IAVS), Cobertura Vegetal (ICV) e Percentual de Cobertura Vegetal (PCV). Foram obtidos os seguintes resultados: 11 Parques de Vizinhança e 5 Parques de Bairro, a praça Waldir Lins apresentou a maior área e o setor com maior área verde foi o Central. O IAVT calculado foi de 1,46, o IAVPV foi de 0,58, o IAVPB foi de 0,88, o maior IAVS foi observado no setor Jardim São Lucas e o menor no setor Central, o ICV foi de 0,48 e o PCV de 0,13.This study aimed to investigate the public squares of Gurupi through index calculations of green areas and vegetation index. Using tape measure were obtained the measures of the squares and the projection of the treetops. Through the Google Earth program were determined the Neighborhood areas and urban perimeter. By the measures, it was made the classification of squares as Vicinity Park and Neighborhood Park. Subsequently were carried out the calculations of the indexes: total green area (IAVT), green area for vicinity parks and neighborhood parks (IAVPV and IAVPB), green area per sector (IAVS), vegetation cover (ICV) and percentage of vegetation cover (PCV). The following results were obtained: 11 vicinity parks and 5 neighborhood parks, the Waldir Lins square presented the largest area and Central sector presented the largest green area. The calculated IAVT was 1.46, the IAVPV was 0.58, the IAVPB was 0.88, was observed the highest IAVS in Jardim São Lucas sector and the lowest in the Central sector, ICV was 0.48 and PCV 0.13

The Southern Photometric Local Universe Survey (S-PLUS) : improved SEDs, morphologies, and redshifts with 12 optical filters

The Southern Photometric Local Universe Survey (S-PLUS) is imaging~300 deg2 of the celestial sphere in 12 optical bands using a dedicated 0.8 m robotic telescope, the T80-South, at the Cerro Tololo Inter-american Observatory, Chile. The telescope is equipped with a 9.2k×9.2k e2v detector with 10μm pixels, resulting in a field of view of 2 deg2 with a platescale of 0.55 arcsec pixel−1. The survey consists of four main subfields, which include twonon-contiguous fields at high Galactic latitudes (|b|>30◦, 8000 deg2) and two areas of the Galactic Disc and Bulge (for an additional 1300 deg2). S-PLUS uses the Javalambre 12-band magnitude system, which includes the 5ugrizbroad-band filters and 7 narrow-band filters centred on prominent stellar spectral features: the Balmer jump/[OII], Ca H+K, Hδ, Gband, Mg b triplet, Hα, and the Ca triplet. S-PLUS delivers accurate photometric redshifts (δz/(1+z)=0.02 or better) for galaxies withr<19.7 AB mag andz<0.4, thus producing a 3D map of the local Universe over a volume of more than 1 (Gpc/h)3. The final S-PLUS catalogue will also enable the study of star formation and stellar populations in and around the MilkyWay and nearby galaxies, as well as searches for quasars, variable sources, and low-metallicity stars. In this paper we introduce the main characteristics of the survey, illustrated with science verification data highlighting the unique capabilities of S-PLUS. We also present the first public data release of ~336 deg2 of the Stripe 82 area, in 12 bands, to a limiting magnitude ofr=21, available atdatalab.noao.edu/splus

Predição temporal de parâmetros da qualidade da água usando redes neurais profundas / Temporal prediction of water quality parameters using deep neural networks

The quality of the water is directly related to its level of pollution, and for that, monitoring is necessary to identify the physical, chemical and biological characteristics, considering the current legislation. This paper presents a comparison between the Long-Short Term Memory (LSTM) and Perceptron Multilayer (MLP) neural network models to predict pH, OD, BOD, Phosphorus, Turbidity, Temperature, Solid, and faecal coliforms of water quality. The error metrics MAPE, RMSE and MSE were used, when the neural networks are configured with 10, 25 and 50 neurons. In five water quality parameters, the LSTM network presented an average RMSE of 0.21, an average MSE of 0.11 and an average MAPE of 5.68. The MLP network showed an average RMSE of 0.21, an average MSE of 0.10 and an average MAPE of 5.55. The results of the experiments aim to contribute to the process of monitoring water quality and to help planning water management through the appropriate machine learning model for predicting parameters. 

Treatment Of Bone Pain Secondary To Metastases Using Samarium-153-edtmp.

More than 50% of patients with prostate, breast or lung cancer will develop painful bone metastases. The purpose of treating bone metastases is to relieve pain, reduce the use of steroids and to maintain motion. To evaluate the use of samarium-153-EDTMP (153Sm-EDTMP) for the treatment of bone pain secondary to metastases that is refractory to clinical management. Retrospective. Division of Nuclear Medicine, Universidade Estadual de Campinas (Unicamp). Fifty-eight patients were studied (34 males) with mean age 62 years; 31 patients had prostate cancer, 20 had breast cancer, three had lung cancer, one had lung hemangioendothelioma, one had parathyroid adenocarcinoma, one had osteosarcoma and one had an unknown primary tumor. All patients had multiple bone metastases demonstrated by bone scintigraphy using 99mTc-MDP,and were treated with 153Sm-EDTMP. Response to treatment was graded as good (pain reduction of 50-100%), intermediate (25-49%) and poor (0-24%). All patients showed good uptake of 153Sm-EDTMP by bone metastases. Among the patients with prostate cancer, intermediate or good response to therapy occurred in 80.6% (25 patients) and poor response in 19.4% (6). Among the patients with breast cancer, 85% (17) showed intermediate or good response to therapy while 15% (3) showed poor response. All three patients with lung cancer showed poor response to treatment. The lung hemangioendothelioma and unknown primary lesion patients showed intermediate response to treatment; the osteosarcoma and parathyroid adenocarcinoma patients showed good response to treatment. No significant myelotoxicity occurred. Pain control is important for improving the quality of life of patients with advanced cancers. The mechanism by which pain is relieved with the use of radionuclides is still not yet completely understood, however, the treatment is simple and provides a low risk of mielotoxicity. Treatment with 153Sm-EDTMP can control the pain secondary to bone metastases effectively in most patients with breast and prostate cancer without significant side effects.122208-1

Fibrodisplasia ossificante progressiva: relato de caso

Fibrodysplasia ossificans progressiva is a rare genetic disease characterized by widespread soft tissue ossification and congenital stigmata of the extremities. We report on a male child followed for ten years since the age of 3 years and 9 months, when the diagnosis was made. He was born with bilateral hypoplasic hallux valgus and ventricular septal defect, corrected by transsternal approach when 32 months old. Restriction of neck mobility followed and foci of ectopic ossification appeared. Four crises of disease exacerbation were treated with oral prednisone and/or other antiinflammatory drugs. Sodium etidronate 5 to 10 mg/kg/day was prescribed intermittently during about six years but was discontinued due to osteopenia. The disease course has been relentless, with severe movement restriction including the chest wall. A review showed few similar case reports in the Brazilian literature. We revisit the criteria for diagnosis and the essentials of management and treatment.Fibrodisplasia ossificante progressiva � uma doen�a gen�tica rara, caracterizada por ossifica��o disseminada em tecidos moles e estigmas cong�nitos nas extremidades. Relatamos sobre uma crian�a do sexo masculino, branca, que vem sendo acompanhada desde seus 3 anos e 9 meses quando diagnosticamos a doen�a. Nascida com halux valgo hipopl�sico bilateral e defeito do septo interventricular, submeteu-se a corre��o cir�rgica atrav�s de esternotomia, aos 32 meses de vida. Seguiram-se progressiva restri��o de movimentos cervicais e aparecimento de n�dulos duros e dolorosos em regi�es paravertebrais c�rvico-tor�cicas. Em dez anos, assistimos a quatro epis�dios bem definidos de exacerba��o da doen�a, com acr�scimos de ossifica��o ect�pica. Nessas ocasi�es, prescrev�amos prednisona oral e/ou antiinflamat�rios n�o hormonais. Etidronato de s�dio, 5 a 10 mg/kg/dia, foi utilizado em per�odos de dois ou tr�s meses, com intervalos de dois meses, durante seis anos. Osteopenia obrigou-nos a interromper o uso do quelante. A evolu��o tem sido inexor�vel, com limita��o intensa de movimentos tor�cicos e corp�reos. A revis�o da literatura brasileira revelou poucos casos semelhantes. Enfatizamos os crit�rios para diagn�stico e princ�pios b�sicos de tratamento.34234