Exploring Complex Cellular Membranes:Lipid Modifications in Extremophilic Archaea and Filamentous Fungi

Since the existence of the microbial world, cellular membranes have been essential for the foundation of life. Each domain of life harbors a few signature lipids, and some lipid species are shared among the three domains of life, i.e., the archaea, bacteria, and eukarya. Although membranes have been studied extensively across all domains, much is still unknown about the enzymes involved in lipid modification, especially in archaea. This thesis explores the diversity of lipid species in membranes of archaea and the enzymes(s) involved in their modification

Aspect Based Opinion Mining & Sentiment Analysis

Opinion mining is a relatively new field that refers to the practice of collecting feedback in the form of online reviews and ratings left by users on various topics. Researchers are now able to monitor the states of consciousness of individuals in real-time because to this development. Just lately, a number of research papers for sentiment analysis were implemented, each of which was based on a unique categorization and ranking procedure. However, the amount of time necessary for the newline performing class has not decreased in any way. Sentiment Sensitivity newline word list SST was provided as a solution to the problem of function mismatch in the go-domain sentiment class across the source area and the target domain; however, achieving improved accuracy and identifying distributional similarities of words became less effective as time went on. Hidden Markov’s persistent development may be seen at the beginning. Cosine In order to achieve more effective and clean pre-processing, a method that is conceptually quite similar to HM-CPCS has been devised. The HM-CPCS methodology, which has recently been suggested, makes use of the POS tagger, a variant of which is based on the Hidden Markov algorithm. Evaluations are created using data from a wide variety of different domains. Similar to a newline, the tags that come before and after it compute the possibility of transitions and the existence of the term newline among the tags in order to increase capability. This is done in order to improve capability

Dynamic Classification of Sentiments from Restaurant Reviews Using Novel Fuzzy-Encoded LSTM

User reviews on social media have sparked a surge in interest in the application of sentiment analysis to provide feedback to the government, public and commercial sectors. Sentiment analysis, spam identification, sarcasm detection and news classification are just few of the uses of text mining. For many firms, classifying reviews based on user feelings is a significant and collaborative effort. In recent years, machine learning models and handcrafted features have been used to study text classification, however they have failed to produce encouraging results for short text categorization. Deep neural network based Long Short-Term Memory (LSTM) and Fuzzy logic model with incremental learning is suggested in this paper. On the basis of F1-score, accuracy, precision and recall, suggested model was tested on a large dataset of hotel reviews. This study is a categorization analysis of hotel review feelings provided by hotel customers. When word embedding is paired with LSTM, findings show that the suggested model outperforms current best-practice methods, with an accuracy 81.04%, precision 77.81%, recall 80.63% and F1-score 75.44%. The efficiency of the proposed model on any sort of review categorization job is demonstrated by these encouraging findings

Application of the codon-shuffling method : Synthesis and selection of de novo proteins as antibacterials

Library-based methods of non-rational and part-rational designed de novo peptides are worthy beacons in the search for bioactive peptides and proteins of medicinal importance. In this report, we have used a recently developed directed evolution method called "codon shuffling" for the synthesis and selection of bioactive proteins. The selection of such proteins was based on the creation of an inducible library of "codon-shuffled" genes that are constructed from the ligation-based assembly of judiciously designed hexamer DNA duplexes called dicodons. Upon induction with isopropyl 1-thio-beta-D-galactopyranoside, some library members were found to express dicodon-incorporated proteins. Because of this, the host cells, in our case Escherichia coli, were unable to grow any further. The bactereostatic/lytic nature of the dicodon proteins was monitored by growth curves as well as by zone clearance studies. Transmission electron microscopy of the affected cells illustrated the extent of cell damage. The proteins themselves were overexpressed as fusion partners and subsequently purified to homogeneity. One such purified protein was found to strongly bind heparin, an indication that the interaction of the de novo proteins may be with the nucleic acids of the host cell, much like many of the naturally occurring antibacterial peptides, e.g. Buforin. Therefore, our approach may help in generating a multitude of finely tuned antibacterial proteins that can potentially be regarded as lead compounds once the method is extended to pathogenic hosts, such as Mycobacteria, for example

ProGlycProt: a repository of experimentally characterized prokaryotic glycoproteins

ProGlycProt (http://www.proglycprot.org/) is an open access, manually curated, comprehensive repository of bacterial and archaeal glycoproteins with at least one experimentally validated glycosite (glycosylated residue). To facilitate maximum information at one point, the database is arranged under two sections: (i) ProCGP—the main data section consisting of 95 entries with experimentally characterized glycosites and (ii) ProUGP—a supplementary data section containing 245 entries with experimentally identified glycosylation but uncharacterized glycosites. Every entry in the database is fully cross-referenced and enriched with available published information about source organism, coding gene, protein, glycosites, glycosylation type, attached glycan, associated oligosaccharyl/glycosyl transferases (OSTs/GTs), supporting references, and applicable additional information. Interestingly, ProGlycProt contains as many as 174 entries for which information is unavailable or the characterized glycosites are unannotated in Swiss-Prot release 2011_07. The website supports a dedicated structure gallery of homology models and crystal structures of characterized glycoproteins in addition to two new tools developed in view of emerging information about prokaryotic sequons (conserved sequences of amino acids around glycosites) that are never or rarely seen in eukaryotic glycoproteins. ProGlycProt provides an extensive compilation of experimentally identified glycosites (334) and glycoproteins (340) of prokaryotes that could serve as an information resource for research and technology applications in glycobiology

Petroleum-Based Plastics Versus Bio-Based Plastics: A Review

Plastic needs have expanded along with population growth, industrialization, and urbanization. Plastic is unrivaled due to its useful properties and is used to prepare numerous important goods daily. This paper encloses the different kinds and applications of petroleum-based plastic and the drawbacks related to their use, i.e., its nonbiodegradability which leads to their stay in the environment for a very long time. Additionally, there are not enough effective disposal techniques for the large volume of plastic waste produced; thus, plastic garbage builds up in the environment and endangers it. Limiting the usage of plastic is necessary to protect the environment. This can be done with the help of bioplastic, which is an excellent substitute for plastic. The different kinds of bioplastic and their biodegradability in different mediums, viz., soil compost and aquatic systems, are addressed in this paper. Along this, the different areas of application of bioplastic have been explored. The present study also addresses the underlying mechanism of plastic polymerization and biodegradation and the current status of bioplastics in the global market

High-Pressure Properties of Wolframite-Type ScNbO4

In this work, we used Raman spectroscopic and optical absorption measurements and first-principles calculations to unravel the properties of wolframite-type ScNbO4 at ambient pressure and under high pressure. We found that monoclinic wolframite-type ScNbO4 is less compressible than most wolframites and that under high pressure it undergoes two phase transitions at ∼5 and ∼11 GPa, respectively. The first transition induces a 9% collapse of volume and a 1.5 eV decrease of the band gap energy, changing the direct band gap to an indirect one. According to calculations, pressure induces symmetry changes (P2/c–Pnna–P2/c). The structural sequence is validated by the agreement between phonon calculations and Raman experiments and between band structure calculations and optical absorption experiments. We also obtained the pressure dependence of Raman modes and proposed a mode assignment based upon calculations. They also provided information on infrared modes and elastic constants. Finally, noncovalent and charge analyses were employed to analyze the bonding evolution of ScNbO4 under pressure. They show that the bonding nature of ScNbO4 does not change significantly under pressure. In particular, the ionicity of the wolframite phase is 61% and changes to 63.5% at the phase transition taking place at ∼5 GPa

LiCrO2 under pressure : In-situ structural and vibrational studies

The high-pressure behaviour of LiCrO, a compound isostructural to the battery compound LiCoO, has been investigated by synchrotron-based angle-dispersive X-ray powder diffraction, Raman spectroscopy, and resistance measurements up to 41, 30, and 10 Gpa, respectively. The stability of the layered structured compound on a triangular lattice with R-3m space group is confirmed in all three measurements up to the highest pressure reached. The dependence of lattice parameters and unit-cell volume with pressure has been determined from the structural refinements of X-ray diffraction patterns that are used to extract the axial compressibilities and bulk modulus by means of Birch-Murnaghan equation-of-state fits. The pressure coefficients for the two Raman-active modes, A and E, and their mode-Grüneisen parameters are reported. The electrical resistance measurements indicate that pressure has little influence in the resistivity up to 10 GPa. The obtained results for the vibrational and structural properties of LiCrO under pressure are in line with the published results of the similar studies on the related compounds. Research work reported in this article contributes significantly to enhance the understanding on the structural and mechanical properties of LiCrO and related lithium compounds

Swift Heavy Ion Induced Modification Studies of C60 Thin Films

Modification induced by 110 MeV Ni ion irradiated thin film samples of C60 on Si and quartz substrates were studied at various fluences. The pristine and irradiated samples were investigated using Raman spectroscopy, electrical conductivity and optical absorption spectroscopy. The Raman data and band gap measurements indicate that swift ions at low fluences result in formations that involve multiple molecular units like dimer or polymer. High fluence irradiation resulted in sub-molecular formations and amorphous semiconducting carbon, indicating overall damage of the fullerene molecules. These sub-molecular units have been identified with nanocrystalline diamond and nanocrystalline graphite like formations.Comment: 7 pages, 29 references and 9 figures submitted to J. Appl. Phy