Petroleum Product as Substrate for the Production of Biosurfactive Amphiphlies by Pseudomonas Aeruginosa

Biosurfactant production by Pseudomonas aeruginosa by utilizing diesel as the source of energy and carbon was studied in this research. Biosurfactant-producing strain of the organism was isolated from hydrocarbon-contaminated water and grown in a mineral medium supplemented with diesel. Production of biosurfactant was assayed by monitoring the increase in cell concentration, biosurfactant concentration, emulsification index and decrease in surface tension. Highest level of cell concentration and biosurfactant concentration (3.3^108CFU/ml and 0.0108mg/ml respectively) were obtained at 132hrs. Emulsification index reached its maximum with 33.3% at 108hrs. Surface tension reached its minimum at 120hrs with a value of 1.1^10-6N/m. The results showed that diesel can be utilized by P. aeruginosa to produce biosurfactant and early stationary phase isolates can be used to obtain higher yield. It also revealed the increasing potentiality of microorganisms in the aspect of oil spill cleanup and rapid reclamation of contaminated lands and water bodies.Keywords: Biosurfactant, diesel, surface tension, emulsification index, cell biomass, Pseudomonas aeruginosa

Effects of climate change on pearl millet (Pennisetum glaucum [L. R. Br.]) production in Nigeria

This paper attempts to examine the relationship between climate change and pearl millet production in Nigeria. It discusses the origin, distribution of the species with some of their properties including production constraints in the Sub Saharan Africa and Nigeria in particular. Northern states of Nigeria were found to be the dominant producers of pearl millets with Sokoto state having a total cultivated area of 747,580ha. Pearl millet was also found to be the major crop amongst others, useful for minimizing the adverse effect of climate change, hence facilitating income and food security among farming communities. Major production constraint of the crop in the Sub Saharan Africa, particularly in Nigeria is yield reduction and/or total crop failure caused by erratic seasonal rainfalls, floods, failing soil fertility and poor crop management practices. Biotic stress due to weeds such as Striga spp as well as insect pests and diseases pestilences is a constraint. Therefore, appropriate climate change mitigation efforts to improve pearl millet production and enhance food security should be adopted in the Sub Saharan Africa and Nigeria.Keywords: Climate change, pearl millet, Sub-Saharan Africa, Nigeri

Comparative Studies on the Biosurfactant Production Capacity of Bacillus Subtilis and Pseudomonas Aeruginosa Using Engine Oil and Diesel Respectively as Substrate

The study was carried out to compare the the production capabilities and the biosurfactant activity of the bacteria, Bacillus subtilis and Pseudomonas aeruginosa using engine oil and diesel as the substrates respectively. The test organisms were isolated from engine oil contaminated soil as in the case of the Bacillus subtilis, which was collected from an automobile workshop in Samaru, Zaria and hydrocarboncontaminated water in the case of the Pseudomonas aeruginosa. The medium used for the experiment was a mineral medium supplemented with 2% engine oil and 2% diesel as the sole source of carbon and energy for Bacillus subtilis and Pseudomonas aeruginosa respectively. Production of biosurfactant was assayed by monitoring the increase in cell concentration, biosurfactant concentration, emulsification index and decrease in surface tension. Highest level of cell concentration and biosurfactant concentration (3.3 x 108 CFU/ml and 0.0106mg/ml respectively) were obtained at 144 h for the Pseudomonas aeruginosa using diesel as source of carbon and energy while the highest level of cell concentration and biosurfactant concentration (3.2 x 108 CFU/ml and 0.0096mg/ml respectively) were obtained at 120hrs for the Bacillus subtilis using engine oil as source of carbon and energy. The research show that Pseudomonas aeruginosa using diesel as the sole source of carbon and energy is better for the production of biosurfactant than Bacillus subtilis using engine oil as the sole source of carbon and energy.Key words: Biosurfactant, engine oil, diesel, Pseudomonas aeruginosa, Bacillus subtilis

Antimicrobial-resistant in Escherichia coli isolated from different effluent locations within Ahmadu Bello University, Zaria, Nigeria

The safety of municipal water is increasingly becoming of concern globally. Agricultural activities, industrial and residential effluents and community waste are ways through which water sources are contaminated and resistant bacteria can be spread via effluents to municipal water. The study aimed to isolate and determine the distribution of antimicrobial drug-resistant Escherichia coli from different points of the University sewer system in April 2018. A total of 48 samples were collected twice weekly from the six randomly selected inspection chamber sites out of the 14 identified sites. The selected sites of the sewer were located in some hostels, markets and health service areas within the ABU. main campus. The samples were processed by culturing on an EMB agar plate followed by biochemical characterization using conventional biochemical tests and Microbact 12E. An antimicrobial sensitivity test was also carried out using 13 different antibiotic discs. The results obtained revealed that the Community market had an isolation rate of 4(50%), while Sickbay had 3(37.5%) and Danfodiyo hostel with 2(25%). Multiple antimicrobial resistance index (MARI) was found to be 0.31 from four isolates (36%) of E. coli of which 3(75%) were sampled from Sickbay and 1(25%) from ABU Dam. Also, five isolates (45%) had MARI of 0.23, of which 2(40%) were sampled from Danfodiyo hostel, 1(20%) from Ribadu hostel and 2(40%) from Community market. The E. coli isolates were more resistant to Ampicillin, tetracycline and cephalothin. Other bacteria isolated were Klebsiella ozaenae, Hapnea alvei and Morganella morganii all with MARI of 0.31. There is a need for public health awareness on the effect of discharging antibiotic-resistant E. coli contaminated effluent into the environment and water bodies. Hence, the public health significance of recycling such water for domestic usage and agricultural purpose

Prevalence of Entamoeba histolytica among patients with gastroenteritis attending some selected hospitals in Zaria Metropolis, Kaduna State

Background: Amoebiasis is a cosmopolitan infection caused by Entamoeba histolytica and ranks third among parasitic infections that lead to death especially in children. Clinical features of amoebiasis range from asymptomatic colonization of amoebic colitis (dysentery or diarrhoea) and invasive extra intestinal amoebiasis, which is manifested most commonly in the form of liver abscesses. Aim: The prevalence of E. histolytica infection in patients having gastroenteritis attending three selected hospitals in Zaria, Kaduna state was studied using direct wet mount and formol-ether concentration method. The hospitals were Hajiya Gambo Sawaba General Hospital Kofan Gayan (Gambo Sawaba), Major Ibrahim B. Abdullahi Memorial Hospital Sabon Gari, Zaria (Limi hospital) and University Health Services ABU Zaria (Sick Bay). Methods: One hundred and forty stool samples from gastroenteritis patients of all ages attending the selected hospitals were examined for E. histolytica trophozoites/cysts via direct wet mount and formol-ether concentration method. Results: Eight samples were positive giving an overall prevalence of 5.71%. Highest prevalence (10.4%) was obtained from patients attending Gambo Sawaba Hospital. The prevalence of 2.4% and 4.0% were recorded among gastroenteritis patients attending Limi Hospital and University (A.B.U, Zaria) health service respectively. With respect to age, the prevalence (11.4%) was highest in the age group 11-15 (years) followed by 6-10 years with prevalence of 6.38%. However, none of the socio-demographic factors examined showed significant statistical association (P>0.05)

Antibiotic susceptibility profile of uropathogens isolated from pregnant women in selected hospitals in Ilorin

Background: Urinary tract infection (UTI) is one of the most common infections among pregnant women and if untreated could lead to serious complications.Objective: This work was carried out to determine the prevalence rate of Enterobacteriaceae uropathogens and their antibiotics susceptibility profile among pregnant women attending two ante-natal clinics in Ilorin, Nigeria.Materials and Methods: A total of 111 pregnant women between the ages of 18-50 attending ante-natal clinic at University of Ilorin Teaching Hospital and Civil Service Hospital, Ilorin participated in the study. Mid-stream urine samples were collected and cultured on Cysteine-Lactose-Electrolyte Deficient agar. Presence of significant bacteriuria (> 105 cfu/mL) was determined using the plate count method. Antibiotic susceptibility testing was done using Kirby-Bauer disk diffusion technique.Results: Of the 111 urine samples collected, 27.9% of them were found positive on culture. Among the isolated organisms, Klebsiella oxytoca (25.7%) followed by Klebsiella pneumonia (22.9%), were the most prevalent. Isolated bacteria were resistant to at least one antibiotic with the highest resistance seen with amoxicillin (94.3%), streptomycin (77.1%) and nitrofurantoin (54. 3%). Amoxicillin-clavulanic acid (51.4%), ceftriaxone (51.4%), ceftazidime (34.3%), cotrimoxazole (51.4%), imipenem (2.9%), ciprofloxacin (14.3%) and gentamicin (25.7%) have the lowest rate of bacteria resistance. Of the isolates, 82.9% showed Multi-Antibiotic Resistance Index (MARI) ≥ 0.3.Conclusion: High prevalence of bacteriuria in both symptomatic and asymptomatic pregnant women was observed in the study areas. The alarming rate of multi-antibiotic resistance strain is therefore an indicator of a serious clinical problem in the community.Keywords: Bacteriuria, pregnancy, urine culture, antibiotic susceptibilit

Sustainable competitive advantage (sca) analysis of furniture manufacturers in malaysia: normalized scaled critical factor index (NSCFI) approach

The purpose of this paper is to investigate Malaysian furniture industry via Sustainable competitive advantages (SCA) approach. In this case study, sense and respond method and Normalized Scaled Critical Factor Index (NSCFI) are used to specify the distribution of companies’ resources for different criteria and detect the attributes which are critical based on expectation and experience of companies’ employs. Moreover, this study evaluates Malaysian furniture business strategy according to manufacturing strategy in terms of an-alyzer, prospector and defender. Finally, SCA risk levels are presented to show how much company’s resource allocations support their business strategy. This case study involved four furniture manufacturing companies, in the southern part of Peninsular Malaysia to provide the overall view of their strategies in the perspective of knowledge & technology management, processes & work flows, organizational, and informa-tion systems. Hence, the findings of this study presented the preliminary results from these furniture companies in Malaysia, which are involved in sustainable competitive advantage (SCA) studies in terms of expectation and experience, NSCFI, operational strategy triangle of prospector (P), defender (D), and analyzer (A). The result shows that almost all fur-niture companies are categorized into the Analyzer type strategy and planning to remain being Analyzer in future to come

Spatial modelling indicates Striga seedbank density dependence on rainfall and soil traits in the savannas of northern Nigeria

Striga is one of most notorious weeds devastating crop production in the dry savannas of northern Nigeria. The weed attacks most cultivated cereals and legumes with crop losses as high as 100% when no control measure is employed. Studies conducted in the dry savannas of Nigeria indicated that Striga seedbank is strongly related to soil and climate properties. This study was conducted to model Striga hermonthica seedbank zones in the dry savannas of Nigeria based on soil and climate properties of the areas. Using multi-stage spatial sampling, 169 soil samples were collected at the centroids of 25 25 km grids across the study area and analysed for physico-chemical properties. The number of Striga seeds were counted from the soil samples using water elutriator and potassium bicarbonate method. Daily temperature, relative humidity and rainfall for each point were downloaded from Climate Hazards Group InfraRed Precipitation with Station data (CHIRPS). Minimum and maximum temperatures, and relative humidity were accessed from National Aeronautics and Space Administration (NASA POWER). Thresholds of various soil and climate variables for optimum concentration of Striga seedbank were analysed using boundary line analysis (BLA). From the BLA, optimum amount of rainfall for high Striga seedbank was 549 mm per annum. While temperature has a wide suitability range for Striga seedbank development. Principal component analysis was used to reduce dimensionality of the dataset into principal components (PCs). Seven PCs which explained 75.6% variation in the data were retained and used in the weighed overlay modelling (WOM). The weighted overlay map produced five distinct Striga seedbank zones; very low, low, moderate, high and very high. More than 60% of the study area had moderate to high Striga seedbanks. The zones vary mostly based on soil, climate and Striga seed count. The establishment of the optimum levels of the environmental factors at which Striga seedbank is favoured will assist in designing a more site-specific Striga management. However, for scalability purpose, adoption of the Striga zoning approach can be useful

Comparative study of ectoparasites on Clarias gariepinus and Tilapia zilli obtained from Kasuwan Bera of Kofai Area, Ardo Kola LGA, Mayo Gwoi, and Jalingo LGAs, Taraba State, Nigeria

This work was carried out to compare the ectoparasites on Clarias gariepinus and Tilapia zilli obtained from Advanced Teachers College (ATC) Kofai Fish Market (Kasuwan Bera), Ardo Kola LGA, Mayo Gwoi, and Jalingo LGAs, Taraba State, from July to November 2016. A total of 80 randomly selected fishes (C. gariepinus and T. zilli) obtained from fish market were examined for ectoparasites. The skin, fins, nostrils, operculum, and buccal cavities scrapping were also examined under a dissecting microscope with dark background. The methods of obtaining parasites were made separately for fish samples from different markets. Fishes were examined for ectoparasites in relation to their size and sex. Of the 80 fish species examined 65(81.25%) were infested. Prevalence of ectoparasite infestation on C. gariepinus and T. zilli were 72.5% and 90% respectively. The male fish has higher percentage infestation (58.82%) than the females (42.18%). Fish with size range of 1.0-9.9 cm recorded the lowest infestation. T. zilli with size range 10.0-19.9 cm had high prevalence of 91.9% infestation. The higher rate of parasite infestation on T. zilli could be attributed to the presence of scale which aids the parasite to hide under. Ectoparasites has no direct effect to consumer, but has effect on fish farms which causes hemorrhagic lesions on the skin making it unappealing to consumers. Gills’ necrosis can result to opportunistic infections by pathogens such as bacteria, viruses and fungi.Keywords: Ectoparasites; fishes; gills’ necrosis