Lower activated partial thromboplastin time prevalence study, in prof. Doutor Fernando da Fonseca Hospital population

Introdução: A doença tromboembolica venosa (TEV) constitui um problema de grande impacto na saúde pública pela sua morbilidade e mortalidade. Tem uma etiologia multifactorial e os factores de risco são aditivos. Segundo vários estudos publicados a determinação de um tempo de tromboplastina parcial activado (TTPa) baixo, associa-se per se a um risco au- mentado de TEV. Objectivo: Cálculo da prevalência dos valores de TTPa inferiores ao Valor de Referência, VR, (excluíram-se utentes com trombofilias hereditárias/adquiridas). Materiais e Métodos: Realizou-se um estudo analítico observacional e longitudinal, no espaço temporal de Abril a Maio de 2014. Definiram-se três populações: 1ª - Dadores de sangue, (15 homens, 15 mulheres), para cálculo do VR, (colabora- ção do Serviço de Imunohemoterapia do Hospital Prof. Doutor Fernando Fonseca, E.P.E. (HFF). 2ª - Casuística dos utentes adultos em ambulatório, com prescrição de TTPa, no espaço temporal definido, 12943. 3ª População – Utentes com uma razão de TTPa <0.95 (razão = valor utente/valor referência) excluíram-se condições hereditárias/adquiridas potencialmente interferentes na hemostase. Utilizou-se o coagulometro BCS-XP® e o reagente TTPa ACTIN FS® da rotina do Laboratório de Hematologia. Cálculos no Excel para Windows 2010 Resultados 1.Cálculo da média: 24,4s; Intervalo de Confiança (90%): 20,6 a 29,5 s 2. Estudo precisão do reagente para TTPa ACTIN FS® Média 24,38 Variância 0,03 Mediana 24,36 Nível de confiança 90,0% 0,06 Desvio-padrão 0,17 Erro-Padrão 0,04 3.Cálculo de prevalências: Casuística dos TTPa: 12943 utentes, prevalência dos TPPa baixos na população, 5,9%. Preva- lência dos TPPa com uma razão <0.95 em utentes sem alterações da hemostase adquridas/hereditárias identificadas, 1,9%. Conclusões: Obtivemos uma prevalência de 1,9% de utentes (sem alterações da hemostase adquiridas/hereditárias identi- ficadas) com TTPa abaixo da razão de 0.95, em 12943 utentes. Sabendo que os valores baixos de TTPa se correlacionam com um risco aumentado de doença tromboembolica, pensamos que, este teste simples e de baixo custo, possa vir a ser utilizado no futuro, em sinergia com outros factores de risco na sua avaliação

Predictive value of heidelberg retina tomograph parameters for the development of glaucoma in the European glaucoma prevention study

PURPOSE: To determine whether baseline Heidelberg Retina Tomograph (HRT) measurements of the optic disc are associated with the development of open-angle glaucoma (OAG) in individuals with ocular hypertension in the European Glaucoma Prevention Study (EGPS). DESIGN: Retrospective analysis of a prospective, randomized, multicenter, double-masked, controlled clinical trial. METHODS: There were 489 participants in the HRT Ancillary Study to the EGPS. Each baseline HRT parameter was assessed in univariate and multivariate proportional hazards models to determine its association with the development of OAG. Proportional hazards models were used to identify HRT variables that predicted which participants in the EGPS had developed OAG. Development of OAG was based on visual field and/or optic disc changes. RESULTS: At a median follow-up time of about 5 years, 61 participants developed OAG. In multivariate analyses, adjusting for randomization arm, age, baseline IOP, central corneal thickness, pattern standard deviation, and HRT disc area, the following HRT parameters were associated with the development of OAG: the "outside normal limits" classification of the Frederick Mikelberg (FSM) discriminant function (hazard ratio [HR] 2.51, 95% confidence interval [CI]: 1.45-4.35), larger mean cup depth (HR 1.64, 95% CI: 1.21-2.23), cup-to-disc area ratio (HR 1.43, 95% CI: 1.14-1.80), linear cup-to-disc ratio (HR 1.43, 95% CI: 1.13-1.80), cup area (HR 1.33, 95% CI: 1.08-1.64), smaller rim area (HR 1.33, 95% CI: 1.07-1.64), larger cup volume (HR 1.30, 95% CI: 1.05-1.61), smaller rim volume (HR 1.25, 95% CI: 1.01-1.54), larger maximum cup depth (HR 1.18, 95% CI: 1.01-1.36), and cup shape measure (HR 1.18, 95% CI: 1.01-1.36). CONCLUSIONS: Several baseline HRT parameters, alone or in combination with baseline clinical and demographic factors, were significantly associated with the development of OAG among the EGPS participants

Extractive Dearomatization of Naphthalane Oil Fraction with Ionic Liquid and -N-Methyl-2-Pyrrolidone

In the article are given an analysis of the results of researches carried out for the purpose of selective treatment of the Naphthalane oil fraction boiling at 260-3400C with ionic liquid (IL) -morfolinphormiate synthesized on the basis formic acid + morpholine and - N-methyl-2-pyrrolidon (N-MP) and  as an extractant. The aim is to remove poisonous components -  sulfur compounds and toxic carcinogenic polynuclear aromatic hydrocarbons in order to improve therapeutic properties of Naphthalane oil cut. For this purpose we have used extraction method. On the basis of the conducted researches the role of -N-methyl-2-pyrrolidone and IL-morpholinephormiatee in extraction conditions have been determined in selective treatment of Nafthalan oil, according to the results of spectral analysis residual amount of aromatic hydrocarbons decreased from 18.5% wt accordance to 2%, 1.5%. As well as the amount of sulphur decreased from 0.0354% to 0.010%,  0.011% and 0.019%.In the next stage the treated raffinates with N-methyl-2-pyrrolidone has been purified on silica. In a result amount of aromatic hydrocarbons drastically decrease to be 0.07% and 0.02%. As a result we aquire transparency and at the same time the percentage amount of aromatic hydrocarbons drastically decreased from 2% to 0,07%, 1.5%, to 0.02%. Raffinates obtained in a 2-4 stage dearomatization with N-MP and IL. Raffinate are measured on NMR, UV and IR spectral analysis

ОПРЕДЕЛЕНИЕ МИКРОКОЛИЧЕСТВ ЖЕЛЕЗА В ФРУКТАХ

For determining the method of photometric used the reaction of Fe with 1-phinil 2,3-dimethil pirazol 5 azo-piraqalol with 1-10 phenantralin and α,α’-dipiridil. The complex product which is getting from the reaction of Fe with reagents shows and prove that, by existing hydrophobic amines, we get different ligand complex and at this time, shift to absorption spectrum happens bathochrome replacement and in the compare with the same ligand’s complex getting maximal complex which shift to acidic medium. We investigate the influence of ligand formation with time and temperature differences and with changing concentration of interaction of components and obtained by different ways, stoichiometry coefficient. We obtained the involvement of Fe in obtaining of 1-10 phenantralin has high selectivity, also this methods is involved to determine the micro-quantity of Fe in fruits: white and red cherry, in strawberry.Key words: Iron (III), nitro-compounds, different ligand’s complex, 1-10 phenantralin.(Russian)DOI: http://dx.doi.org/10.15826/analitika.2013.17.1.015H.D. Naghiyev, F.E. Esphandi*, R.A. Aliyeva, U.A. Gullarli, F.M. ChiraqovBaku State University, Azerbaijan, Baku*Islamic  Azad University, Karaj Branch Разработаны методики фотометрического определения железа(III) с 1-фенил-2,3-диметилпиразолон-5-азопирогаллолом в присутствии 1,10-фенантролина и α,α'-дипиридила. Исследовано комплексообразование железа(III) с реагентом и установлено, что в присутствии гидрофобных аминов образуются разнолигандные комплексы, при этом наблюдаются батохромные сдвиги в спектре поглощения и максимальный выход сдвигается в кислую среду по сравнению однороднолигандным комплексом. Изучено влияние концентрации реагирующих компонентов, времени и температуры на образование разнолигандных комплексов и определена их стехиометрия различными методами. Показано, что определение железа(III) с реагентом в присутствии 1,10-фенантролина высокоизбирательно. Разработанная методика применена для определения микроколичеств железа в фруктах: в белой и красной черешне и в  клубнике.Ключевые слова: железо(III), азосоединения, разнолигандный комплекс,    1,10-фенантролинDOI: http://dx.doi.org/10.15826/analitika.2013.17.1.01

Loss of MED23 leads to poor prognosis in invasive breast cancer

Purpose of the study: The molecular mechanism of lymphovascular invasion (LVI) which determines the early metastatic phenotype in breast cancer is still not fully understood. Lead from the METABRIC study revealed that MED23 correlated with negative LVI status (p=0.00005). Hence MED23 expression was studied at the protein level for correlations with LVI and other clinical-pathological parameters. Methods: The METABRIC BC cohort (n=1980) was evaluated for MED23 mRNA expression and prognostic impact externally validated using the online bc-GenExminer 4.0. Correlation between MED23 protein expression with clinicopathological parameters, patient outcome and other biomarkers were explored (Nottingham Tenovus series; n=1255) using immunohistochemistry (IHC). Results: High MED23 mRNA expression was negatively associated with tumour stage and was differentially expressed in good prognosis integrative clusters 7 and 8 (p<0.001). MED23 IHC revealed nuclear expression (n-MED23). Although no association was found with LVI, higher n-MED23 expression correlated with low NPI, low grade, older age, ER+ status, low Ki67 index and low N-cadherin expression (p<0.05). Positive correlations with PTEN, GATA3, STAT3 and CDC42 (p<0.001), indicate possible interacting pathways. In univariate analysis, high n-MED23 expression showed better long-term patient outcome in the whole cohort and ER+ subgroups (p<0.05). Pooled MED23 expression in an external validation cohort (ER+LN-) also showed association with better patient outcome (p<0.02, HR=0.82, 95% CI 0.69-0.98). Conclusion: Results of the study suggest that loss of n-MED23 is a marker of poor prognosis in invasive BC, results re-enforced by expression data. The difference in correlation with LVI at gene and protein level highlights the importance of IHC validation and indicates MED23 as a probable bystander in the LVI cascade

Obesity exacerbates influenza-induced respiratory disease via the arachidonic acid-p38 MAPK pathway

Obesity is a risk factor for severe influenza, and asthma exacerbations caused by respiratory viral infections. We investigated mechanisms that increase the severity of airway disease related to influenza in obesity using cells derived from obese and lean individuals, and in vitro and in vivo models. Primary human nasal epithelial cells (pHNECs) derived from obese compared with lean individuals developed increased inflammation and injury in response to influenza A virus (IAV). Obese mice infected with influenza developed increased airway inflammation, lung injury and elastance, but had a decreased interferon response, compared with lean mice. Lung arachidonic acid (AA) levels increased in obese mice infected with IAV; arachidonic acid increased inflammatory cytokines and injury markers in response to IAV in human bronchial epithelial (HBE) cells. Obesity in mice, and AA in HBE cells, increased activation of p38 MAPK signaling following IAV infection; inhibiting this pathway attenuated inflammation, injury and tissue elastance responses, and improved survival. In summary, obesity increases disease severity in response to influenza infection through activation of the p38 MAPK pathway in response to altered arachidonic acid signaling

Quaternary time scales for the Pontocaspian domain: interbasinal connectivity and faunal evolution

The Pontocaspian (Black Sea - Caspian Sea) region has a very dynamic history of basin development and biotic evolution. The region is the remnant of a once vast Paratethys Sea. It contains some of the best Eurasian geological records of tectonic, climatic and paleoenvironmental change. The Pliocene-Quaternary co-evolution of the Black Sea-Caspian Sea is dominated by major changes in water (lake and sea) levels resulting in a pulsating system of connected and isolated basins. Understanding the history of the region, including the drivers of lake level and faunal evolution, is hampered by indistinct stratigraphic nomenclature and contradicting time constraints for regional sedimentary successions. In this paper we review and update the late Pliocene to Quaternary stratigraphic framework of the Pontocaspian domain, focusing on the Black Sea Basin, Caspian Basin, Marmara Sea and the terrestrial environments surrounding these large, mostly endorheic lake-sea systems