Role of MicroRNA in Breast Cancer

Breast Cancer is the commonest cancer affecting women worldwide especially in Asia. Several proteinaceous,genetic andepigenetic biomarkers areallied with the disease but their efficacy as vigorous and robust indicators of disease remains uncertain.The need to detectand differentiate aggressive from non-aggressive breast tumors at cellular level is being investigated.MicroRNAs seem to be a promising marker to identify the disease before it reaches aggressive level. MicroRNAs (miRNAs)are small 18-24 nucleotide RNAs which regulate the expression of approximately 30% of human genes. Their expression isfrequently deregulated in cancers. .miRNAshave been found in significantly large copy numbers in serum/plasma of cancerpatients. The stability of the serum miRNAs is not compromised even if the samples are treated with RNase or incubated atroom temperature over prolong periods or subjected to repeated freeze-thaw cycles. miRNAs that are breast cancer-specificcan therefore be employed as disease predicting biomarkers.Keywords:Breast cancer, Micro RNA, Role, Serum, Stability

An open-source software framework for the integrated simulation of structures in fire

The traditional methods to understand the development of elevated temperature in a structure, and also the associated structural response, are not representative of realistic fire scenarios. To provide a more accurate and realistic reflection of the fire development, the current paper develops a generic middleware which interfaces between the computational fluid dynamics (CFD) software Fire Dynamics Simulator (FDS) and the finite element (FE) analysis software OpenSees. This framework enables a fully integrated simulation of a realistic fire scenario including the heat transfer through the structure and the resulting thermo-mechanical response. The proposed framework is open-source and freely available and therefore can be used and further developed by researchers and practicing engineers and customised to their requirements. This paper shows validation against two sets of experimental results and one real fire incident. A number of different types of thermal boundary conditions such as gas temperatures and heat fluxes, are obtained from the CFD analysis and are then used in the subsequent heat transfer and thermo-mechanical analysis. The primary advantage of this computational tool is that it provides consultants and designers with the means to undertake large-scale projects requiring performance-based fire engineering solutions

Bowled over by cricket: impact of tape-ball injuries on the eyes

Abstract PURPOSE: The aim of this study was to describe the type and severity of tape-ball cricket-related eye injuries seen at a tertiary care hospital. DESIGN: A descriptive case series. METHODS: This study included all cases of tape-ball cricket-related eye injuries presenting to the Section of Ophthalmology, Aga Khan University Hospital, Karachi, from January 2014 to January 2015. RESULTS: A total of 20 patients with tape-ball cricket-related eye injuries were treated during this period. The right eye was involved in most (14/20) of the cases. The commonest presenting symptoms included reduced/blurred vision, ocular pain, redness, and floaters. Sixty percent (12/20) of the affected eyes had a best corrected visual acuity of less than 20/200 at presentation, with the retina being the most commonly involved ocular structure. All patients reported that they were not wearing protective eyewear at the time of trauma. Despite standard management, half of the eyes had severe or total vision loss at the last follow-up (minimum, 3 months), mainly due to retinal damage. CONCLUSIONS: Our study calls for the compulsory use of protective eyewear by cricket players and for countrywide surveillance data on the true burden of this unnoticed preventable cause of unilateral eye injuries and associated vision loss

The Experimental Study On the Effectiveness of Social Media Ad Campaign: Like, Comment, Share

The popularity of social media outlets has forced us to inquire about marketing effectiveness in any area of the industry. Social media has rapidly risen in popularity as a new advertising platform that allows users to connect with one another and engage with brands.  This research has attempted to explore the attitude of people over the advertisements in what formats most people prefer to like, comment or share. We focus top trended social media sites Facebook, Twitter and LinkedIn to demonstrate the consumer engagement on advertisements placed on these sites. This research has identified the factors which influence the users to keep an eye out on advertisements that make them want to continue watching it as they spend their daily time on these social media websites and apps. To determine what makes Ads and online marketing campaigns successful we used variables such as Vividness, Content of Posts, Position of Posts, Scheduling and Call to Action.  It was conducted on 300 respondents. We used the questionnaire to collect the data and used a semantic differential scale. We questioned about the ads to respondents which were based on our variables. Results showed that our research is decisive but the variable call to action has an insignificant effect and other variables such as vividness, the position of post, the content of the post, schedule have a significant relation with customer engagement

Efficacy of Entomopathogenic Fungi as Biological Control agent against insect pests of Gossypium hirsutum.

Isolates of Beauveria bassiana, Metarhizium anisoplaie, Verticillium  lecanii and Paeciliomyces lilicuns from Punjab, Pakistan, were evaluated for their inhibitory and insecticidal efficacy against White fly, House fly, American bollworm, Army worm, Spotted worm, Gray weevil, Jassids, Aphids, Ant and moth. Four strains of B. bassiana, two strains of P. lilicuns and one strain of M. anisoplaie and V. lecanii were used. B.bassiana (Bb04) exhibited significant mortality percentage against moth, gray weevil, cotton seed bug white fly, American worm, spotted worm, army worm, house fly, ants, jassids and aphids. Among all the strains of entomopathogenic fungi used, B. bassiana strains Bb01 revealed least mortality percentage against the targeted insect pests. V. lecanii Vl01 strain showed the highest mortality rate against cotton seed bug while it showed least efficiency against spotted worm. M. anisopolie Ma01 showed the highest mortality percentage against moth and showed the minimum results against spotted worm. In case of tested strain of P. lilicuns strains Pl01 and Pl02 displayed the highest mortality percentage against gray weevil, cotton seed bug and showed the minimum results against spotted worm and moth. Key words: Entomopathogenic fungi, Pathogenicity, Cotton pests, White fly, American bollworm, Army worm, Gray weevi

Yield of esophagogastroduodenoscopy and colonoscopy in cancer of unknown primary

Objectives: Carcinoma of unknown primary origin (CUP) is heterogeneous group of cancers. Role of gastrointestinal (GI) endoscopy in this entity is under investigated. Aim of this study was to evaluate yield of Colonoscopy and Esophagogastroduodenoscopy (EGD) in localizing primary tumor in patients with CUP. METHODOLOGY: Patients with histopathologically proven CUP who underwent colonoscopy / EGD to find the primary tumor from December 2009 to December 2011 were included in the study. Abdominal symptoms and cytokeratin (CK) 7 and 20 markers were correlated with presence of primary in GI tract. Results: After giving informed consent 86 patients were included in final analysis. All patients underwent colonoscopy while 60(70%) got EGD along with colonoscopy. Mean age was 55.10 +/-11.94 years with 52(60%) male. Abdominal symptoms were present in 50%. CK7+/CK20- in 34(40%); CK7-/CK20+ in 2(2%) while CK7+/20+ in 7(8%) of metastatic tumor samples. Liver was metastatic site in 47(55%), Lymph node 12(14%) and Ascites in 8(9%). Endoscopy detected primary in 6 (7%) patients with 3 each in stomach and colon. No association of abdominal symptoms and cytokeratin markers was found with presence of GI primary site. CONCLUSION: Yield of localizing primary lesion in the GI tract by pan-endoscopy was limited. Abdominal symptoms and cytokeratin markers do not predict presence of gastrointestinal malignancies

The impact of disease control measures on the spread of COVID-19 in the province of Sindh, Pakistan

The province of Sindh reported the first COVID-19 case in Pakistan on 26(th) February 2020. The Government of Sindh has employed numerous control measures to limit its spread. However, for low-and middle-income countries such as Pakistan, the management protocols for controlling a pandemic are not always as definitive as they would be in other developed nations. Given the dire socio-economic conditions of Sindh, continuation of province-wise lockdowns may inadvertently cause a potential economic breakdown. By using a data driven SEIR modelling framework, this paper describes the evolution of the epidemic projections because of government control measures. The data from reported COVID-19 prevalence and google mobility is used to parameterize the model at different time points. These time points correspond to the government’s call for advice on the prerequisite actions required to curtail the spread of COVID-19 in Sindh. Our model predicted the epidemic peak to occur by 18(th) June 2020 with approximately 3500 reported cases at that peak, this projection correlated with the actual recorded peak during the first wave of the disease in Sindh. The impact of the governmental control actions and religious ceremonies on the epidemic profile during this first wave of COVID-19 are clearly reflected in the model outcomes through variations in the epidemic peaks. We also report these variations by displaying the trajectory of the epidemics had the control measures been guided differently; the epidemic peak may have occurred as early as the end of May 2020 with approximately 5000 reported cases per day had there been no control measures and as late as August 2020 with only around 2000 cases at the peak had the lockdown continued, nearly flattening the epidemic curve

The world trade center 9/11 disaster and progressive collapse of tall buildings

The collapse of the World Trade Center buildings on September 11, 2001 posed questions on the stability of tall buildings in fire. Understanding the collapse of the WTC Towers offers the opportunity to learn useful engineering lessons in order to improve the design of future tall buildings against fire induced collapse. This paper extends previous research on the modelling of the collapse of the WTC Towers on September 11, 2001 using a newly developed ‘‘structures in fire’’ simulation capability in the open source software framework OpenSees. The simulations carried out are validated by comparisons with previous work and against the findings from the NIST investigation, albeit not in the forensic sense. The column ‘‘pull in’’ that triggers the instability of the structure and leads to collapse is explained. The collapse mechanisms of generic composite tall buildings are also examined. This is achieved through carrying out a detailed parametric study varying the relative stiffness of the column and the floors. The two main mechanisms identified in previous research (weak and strong floor) are reproduced and criteria are established on their occurrence. The analyses performed revealed that the collapse mechanism type depended on the bending stiffness ratio and the number of floors subjected to fire and that the most probable type of failure is the strong floor collapse. The knowledge of these mechanisms is of practical use if stakeholders wish to extend the tenability of a tall building structure in a major fire.Professor Jose Torero and the Open-Sees team at PEER, UC Berkele