Private solid waste collection services, Dar es Salaam, Tanzania

Private solid waste collection services, Dar es Salaam, Tanzani

Development of male sterility by silencing Bcp1 gene of Arabidopsis through RNA interference

The development of male sterility is one of the most important steps in hybrid seed production. Several methods for the abortion of pollens based on conventional as well as genetic engineering are reported for the various crop species. Here we have investigated the use of RNA interference (RNAi) technology to silence a male specific gene, Bcp1 in the model host Arabidopsis thaliana. Bcp1 is active in both diploid tapetum and haploid microspores. Three batches of explants (A. thaliana) were selected onherbicide glufosinate ammonium and putative transgenes were confirmed through PCR and Southern hybridization. About 49 out of 58 Arabidopsis lines transformed with RNAi construct containing Bcp1 sequences were male sterile. Transgenic plants were phenotypically indistinguishable from nontransgenic plants and by crossing with non-transgenic fertile pollens successful seed set was observed. The Bcp1 gene was also amplified from chilies, tomato and brassica. The present study resulted in developing male sterile A. thaliana (Eco. Columbia) line through genetic engineering. The diversity of Bcp1 gene will help us to produce transgenic male sterile plants in crop plants

The response of sugarcane (Saccharum officinarum L) genotypes to callus induction, regeneration and different concentrations of the selective agent (geneticin -418)

Two commercial cultivars (CPF-245 and CPF-237) and three advanced lines (CSSG-668, S-2003US633, S-2003US114) of sugarcane (Saccharum officinarium) grown in Punjab, Pakistan were evaluated for their potential to induce callus, embryogenic callus and regeneration. Cultivar CSSG-668 was found to be the best genotype yielding maximum embryogenic callus and regeneration whereas cultivar CPF- 245 exhibited lowest callus induction frequency. Five different concentrations (0, 20, 40, 60, and 80mg/L) of the selective agent (geneticin-418) were used to optimize selection conditions with nontransformed embryogenic calli. The geneticin concentration 60 mg/L was found to be the optimal dose to select the embryogenic calli of genotypes CSSG-668, CPF-245 and S-2003US63, while 35 mg/L geneticin was found to be the best concentration for S-2003US-114. Similarly, 60 mg/L geneticin was optimum dose to select regenerated plantlets of the cultivars CSSG-668 and CPF-245 while it was 40, 25 mg/L for the cultivars S-2003US-114 and S-2003US-633, respectively. It is concluded from the present study that geneticin concentration in the range of 25 to 60 mg/L can be effectively used for the selection of transformed embryogenic calli and regenerants of different sugarcane cultivars.Keywords: Callus induction, embryogenic callus, regeneration, Saccharum officinarum L., selection, geneticin

A novel cell-selection optimization handover for long-term evolution (LTE) macrocellusing fuzzy TOPSIS

To satisfy the demand for higher data rate while maintaining the quality of service, a dense long-term evolution (LTE) cells environment is required. This imposes a big challenge to the network when it comes to performing handover (HO). Cell selection has an important influence on network performance, to achieve seamless handover. Although a successful handover is accomplished, it might be to a wrong cell when the selected cell is not an optimal one in terms of signal quality and bandwidth. This may cause significant interference with other cells, handover failure (HOF), or handover ping-pong (HOPP), consequently degrading the cell throughput. To address this issue, we propose a multiple-criteria decision-making method. In this method, we use an integrated fuzzy technique for order preference by using similarity to ideal solution (TOPSIS) on S-criterion, availability of resource blocks (RBs), and uplink signal-to-interference-plus-noise ratio. The conventional cell selection in LTE is based on S-criterion, which is inadequate since it only relies on downlink signal quality. A novel method called fuzzy multiple-criteria cell selection (FMCCS) is proposed in this paper. FMCCS considers RBs utilization and user equipment uplink condition in addition to S-criterion. System analysis demonstrates that FMCCS managed to reduce handover ping-pong and handover failure significantly. This improvement stems from the highly reliable cell-selection technique that leads to increased throughput of the cell with a successful handover. The simulation results show that FMCCS outperforms the conventional and cell selection scheme (CSS) methods

Synthesis and Evaluation of Tripodal Peptide Analogues for Cellular Delivery of Phosphopeptides

Tripodal peptide analogues were designed on the basis of the phosphotyrosine binding pocket of the Src SH2 domain and assayed for their ability to bind to fluorescein-labeled phosphopeptides. Fluorescence polarization assays showed that a number of amphipathic linear peptide analogues (LPAs), such as LPA4, bind to fluorescein-labeled GpYEEI (F-GpYEEI). LPA4 was evaluated for potential application in cellular delivery of phosphopeptides. Fluorescence microimaging cellular uptake studies with fluorescein-attached LPA4 (F-LPA4) alone or with the mixture of LPA4 and F-GpYEEI in BT-20 cells showed dramatic increase of the fluorescence intensity in cytosol of cells, indicating that LPA4 can function as a delivery tool of F-GpYEEI across the cell membrane. Fluorescent flow cytometry studies showed the cellular uptake of F-LPA4 in an energy-independent pathway and confirmed the cellular uptake of F-GpYEEI in the presence of LPA4. These studies suggest that amphipathic tripodal peptide analogues, such as LPA4, can be used for cellular delivery of phosphopeptides

Analytical study of reaction diffusion Lengyel-Epstein system by generalized Riccati equation mapping method

In this study, the Lengyel-Epstein system is under investigation analytically. This is the reaction–diffusion system leading to the concentration of the inhibitor chlorite and the activator iodide, respectively. These concentrations of the inhibitor chlorite and the activator iodide are shown in the form of wave solutions. This is a reaction†“diffusion model which considered for the first time analytically to explore the different abundant families of solitary wave structures. These exact solitary wave solutions are obtained by applying the generalized Riccati equation mapping method. The single and combined wave solutions are observed in shock, complex solitary-shock, shock singular, and periodic-singular forms. The rational solutions also emerged during the derivation. In the Lengyel-Epstein system, solitary waves can propagate at various rates. The harmony of the system’s diffusive and reactive effects frequently governs the speed of a single wave. Solitary waves can move at a variety of speeds depending on the factors and reaction kinetics. To show their physical behavior, the 3D and their corresponding contour plots are drawn for the different values of constants

Haematologic Parameters In Acute Promyelocytic Leukemia Patients Treated With ALL Trans- Retinoic acid

Background: Acute Promyelocytic Leukemia (APL) is commonly associated with disseminated intravascular coagulation (DIC) and early correction of coagulopathy is of vital importance. All Trans-Retinoic Acid (ATRA) is considered to be the drug of choice in the treatment of APL.  Objective: The work was conducted to 1- Identify patients with APL who show laboratory evidence of DIC. 2- Study the serial changes in haemostatic parameters in APL patients treated with ATRA and to compare their results with those treated with conventional chemotherapy without ATRA. Subjective and methods: In this prospective study (from October 2003 to October 2005), 44 newly diagnosed, untreated APL patients were included. ATRA plus chemotherapy – treated patients were 24 while 17 patients were treated with chemotherapy other than ATRA. For each patient, a full clinical evaluation was done and hematological investigations were accomplished at time of diagnosis and repeated on day 3 and 7 of therapy. Diagnosis of DIC was based on finding a positive D- dimer test with hypofibrinogenaemia with or without pathologically prolonged (PT and/or APTT). Results: In 44 newly diagnosed, untreated APL patients studied, the age range between 6-81 years with a median of 27 years. Male to female ratio was 1.3:1. Before treatment all patients had anemia, thrombocytopenia, and elevated level of D – dimer. DIC was present in all patients at time of diagnosis. All parameters that showed abnormal level at time of diagnosis had returned to normality within one week in ATRA treated group, indicating that DIC has essentially resolved. By contrast, those parameters remained abnormal even on day 7 in the chemotherapy treated group. Indicating that DIC was on going. Conclusion: ATRA therapy in APL patients is associated with rapid improvement of coagulopathy therefore , it is justified to be used from day one of the treatment

Managing Chronic Diseases in Family Medicine: Best practices and Evidence-Based Approaches

The management of chronic diseases within the realm of family medicine presents a multifaceted challenge with profound implications for healthcare systems and patients alike. Chronic diseases, such as diabetes, hypertension, and cardiovascular conditions, are prevalent and impose a significant burden on individuals, families, and society as a whole. This article explores best practices and evidence-based approaches for managing chronic diseases in family medicine. It delves into the epidemiological landscape of chronic illnesses, emphasizing the need for effective prevention and management strategies. Evidence-based Models, such as The Chronic Care Model (CCM), Patient-Centered Medical Home (PCMH), and Self-assessment models are discussed in the context of family medicine. The importance of comprehensive, coordinated, and patient-centric approaches is underscored, highlighting the pivotal role of primary care physicians in the ongoing battle against chronic diseases. It is clear, that development in the field of family medicine underscores the importance of patient involvement in diseases management process through shared-decision making model. Although such model require physicans to spend more time educating patients so they can make informed decisions and implement self-management strategies, it has overall better health outcomes and eventually needs to requiring less intervention by physicians