Effect of water pollution on expression of immune response genes of Solea aegyptiaca in Lake Qarun

This research was aimed to study quality of water in Lake Qarun and effects of pollution on expression of immune genes in Egyptian sole (Solea aegyptiaca) fish. The study was carried out from August 2006to the end of April 2007. The water samples were collected from different locations of Lake Qarun at Al-Oberge within an area of 200 to 1500 meter from the shore. The samples were subjected to differentphysical and chemical analyses. The concentration of total dissolved solids (TDS) recorded an average value of 37.8 g/l while the chloride content was 14.3 g/l on average. The corresponding value of salinitywas 25.9 g/l. For the chemical oxygen demand and biochemical oxygen demand the results revealed that the average values were 98 ± 22 mg O2/l and 8.0 ± 2.1 mg O2/l for the chemical oxygen demand(COD) and biochemical oxygen demand (BOD), respectively. Analyses of nitrogen group indicated presence of low concentrations of all. For total pesticides which might be one of the most potential pollutants in the lake, the results showed an average value of 0.62 mg/l. Furthermore, the total viable bacterial count (TVBC) ranged from 103 colony forming unit (CFU) in the middle of the Lake to 107 (CFU)near the shore. Stressed total coliform group (STC), stressed faecal coliform group (SFC) and stressed faecal streptococci group (SFS) increased from the middle of the Lake to the shore. The differentialexpression of the immune genes, that is, GARP and SIMP genes, as a result of pollution influence was further confirmed by RT-PCR, with the up-regulation of these genes in the liver of the collected fish. Theapplication of the expression of the immune genes of fish might be time safe and cost effective in case there are different source of pollution

Response of Asymmetric Slim Floor Beams in Parametric-Fires

State-of-the-art slim floor systems are a newest addition to the composite construction industry and several types are currently being used for building and construction purposes. Asymmetric slim floor beams are a type of slim floor systems which consist of a rolled section with a larger bottom flange. The larger bottom flange induces asymmetry and offers an efficient use of the material strength as a composite beam. It also offers a larger area to support the steel decking and pre-cast slab units during the construction of floor. Experimental and analytical investigations on response of asymmetric slim floor beams have shown that these beams offer a higher fire resistance in comparison to the conventional composite systems with down-stand steel beams. Previous investigations on these beams have been conducted in standard fire exposure conditions, hence, their response to natural fire scenarios still deems further examination. This study addresses response of asymmetric slim floor beams in natural fire exposure conditions. For this purpose, finite element models developed and verified by the authors are employed to study the thermal and structural response of slim floor beams in fast and slow parametric-fire exposures. Results obtained show that the asymmetric slim floor beams behave differently in parametric-fires in comparison to that in standard fire exposure conditions. Asymmetric slim floor beams continued to support the loads for the whole duration of parametric fires without undergoing excessive deflections and offering a better fire resistance. Unlike in case of the standard fire where the temperatures keep on increasing throughout the duration, temperatures on the slim floor beams decrease after reaching a maximum point in parametric-fires. It was found that for fast parametric-fires, the thermal gradient across the section is more severe as compared to that for the slow parametric-fires at earlier stages of fire exposure. In case of the fast parametric-fires, the rise and fall of temperatures on the slim floor beams are rapid while in case of the slow parametric-fire, these variations in temperatures are subtle. It was observed that the structural response of slim floor beams in standard and parametric fires depends on the average temperature across the steel section. Deflections predicted for the beams were found to be directly related to these average temperatures. Outcomes of this study will benefit in understanding the response of asymmetric slim floor beams in natural fire conditions and will aid to develop simple fire design methods for future use

Effect of Air-Gap on Performance of Fabricated Slim Floor Beams in Fire

Fabricated slim floor beams are produced by welding a steel plate to the bottom flange of an I-shaped steel section. The welded steel plate makes them structurally efficient and serves as a platform to support the steel decking of composite floor and the pre-cast concrete slabs. During their fabrication, an air-gap is induced between the steel plate and the bottom flange. Previous experimental investigations have shown that this air-gap has an influence on their thermal behaviour at elevated temperatures. Though the air-gap presence has an influence on their thermal performance, no investigations have yet been conducted to analyse its effects on their structural response in fire. This research investigates the effects of air-gap on structural response of fabricated slim floor beams in fire. During this study, finite element modelling is performed to simulate the response of fabricated slim floor beams and the predicted behaviour is verified against the available test data from literature. The validated finite element model is then employed to perform parametric studies to investigate the effects of the presence and size of the air-gap on their response in fire. Results obtained show that the presence of the air-gap has a significant influence on structural response of these beams at elevated temperatures. On the other hand, the size of air-gap has no or negligible effect on their thermal behaviour as well as on their structural response in fire. It was found that the presence of the air-gap restricts temperatures on the bottom flange and helps in achieving an improved fire resistance. As the presence of the air-gap is found to be helpful and beneficial, findings from this research can be used to develop similar designs for structural members as an efficient and inexpensive way to improve their behaviour in fire

Percutaneous transmitral balloon commissurotomy (PTMC): Procedural success and immediate results at Ahmed Gasim Cardiac Center

Background: Balloon valvuloplasty for stenosed mitral and pulmonary valves has been practiced with good results for the last two years in Ahmed Gasim Cardiac Centre, Khartoum Sudan. Objectives: The aim of this study is to audit percutaneous trans-mitral balloon commissurotomy [PTMC] in our current set up. Methods: One hundred and eight patients underwent percutaneous transvenous mitral commissurotomy (PTMC) from April- 2004 to December-2005 in Ahmed Gasim cardiac center. Results: 67% percent of the patients were females. Age range was from 13years to 65years and the mean age was 27years. 27% were under 21 years of age and 12% had special problems. The procedural success was achieved in 94.5% patients, in two patients we failed to dilate a tough septum, in other two the balloon got stuck to the septum and failed to cross the MV, while two patients had successful emergency MVR (due to inadvertent puncture of the RA and cardiac tamponade). There was no mortality related to the procedure, significant mitral regurgitation of > grade 2 didn't occur. No patient developed systemic embolization. Optimal results were achieved in 91.6% patients and in 92% of the patients with special problems. Hemodynamic data in the Cath. Lab showed left atrial mean (±SD) pressure dropped from 32 (±3.2) mmHg to 12 (±2.4) mmHg. LA-LV gradient dropped from an average of 25 to 5 mmHg. Echocardiographic assessment showed mean (±SD) mitral valve area increased from 0.86 (±0.19) cm2 to 1.9 (±0.5) cm2 (

Transparent conductive oxide films for high-performance dye-sensitized solar cells

In this paper, atmospheric pressure chemical vapor deposition of fluorine-doped tin oxide (FTO) thin films of various thicknesses and dopant levels is reported. The deposited coatings are used to fabricate dye-sensitized solar cells, which exhibited reproducible power conversion efficiencies in excess of 10%. No surface texturing of FTOs or any additional treatment of dye-covered films is applied. In comparison, the use of commercial FTOs showed a lower cell efficiency of 7.11%. Detailed analysis showed that the cell efficiencies do not simply depend on the resistivity of FTOs but instead rely on a combination of carrier concentration, thickness, and surface roughness properties

A Genome-wide gene-expression analysis and database in transgenic mice during development of amyloid or tau pathology

We provide microarray data comparing genome-wide differential expression and pathology throughout life in four lines of "amyloid" transgenic mice (mutant human APP, PSEN1, or APP/PSEN1) and "TAU" transgenic mice (mutant human MAPT gene). Microarray data were validated by qPCR and by comparison to human studies, including genome-wide association study (GWAS) hits. Immune gene expression correlated tightly with plaques whereas synaptic genes correlated negatively with neurofibrillary tangles. Network analysis of immune gene modules revealed six hub genes in hippocampus of amyloid mice, four in common with cortex. The hippocampal network in TAU mice was similar except that Trem2 had hub status only in amyloid mice. The cortical network of TAU mice was entirely different with more hub genes and few in common with the other networks, suggesting reasons for specificity of cortical dysfunction in FTDP17. This Resource opens up many areas for investigation. All data are available and searchable at http://www.mouseac.org

Awareness of hypertension guidelines among family physicians in primary health care

Background: Only 14% of patients on treatment achieve the recommended blood pressure target. Guidelines aim to assist clinicians in the management of patients with hypertension.Objectives: The primary purpose of the study was to survey family physicians(FPs) in Kuwait about their awareness, and to understand better their reasons for not implementing specific guidance within the WHO/ISH guidelines.Methods: This study is a cross-sectional survey that was carried out in the five health regions of Kuwait. All PHC physicians who were currently working as FPs were asked to participate in the study. Data were collected using a structured questionnaire of clinically oriented questions formulated on the basis of the 1999 World Health Organization/International Society of Hypertension (WHO/ISH), as standard reference.Results: The study revealed that 49.1% and 42.1% of FPs were very familiar or somewhat familiar with the guidelines respectively, 92.1% were in agreement, and 79.8% indicated that they always or usually follow these guidelines when treating patients. Regarding the correct choice of the guideline statements, only 8.8% of the FPs choose correctly less than ten of the 20 statements, 64% choose 10 to less than 15, and only 27.2% choose > 15 statements. When asked about perceived patient barriers to blood pressure control, 84.0% of the respondents ranked overcrowded clinics as important or most important barrier to blood pressure control while, 87.4% considered lack of patient knowledge as important or most important barrier. Non availability of the drugs in the clinic was considered by 88.4% of the physicians, and poor adherence to antihypertensive drugs by 90.1%.Conclusion: There is a need to establish nationwide educational and quality monitoring programs to facilitate the correct implementation of hypertension guidelines in PHC clinical practices in Kuwait.Keywords:  Family physicians; Awareness; Hypertension; Guideline