Acervo de DOPS/PR : O mapeamento dos dossiês sobre exilio de brasileiros

Fil: Braggio, Ana Karine. Universidade Estadual de Maringá, Brasil.Fil: Fiuza, Alexandre Felipe. Universidade Estadual do Oeste do Paraná, Brasil

Occurrence of social wasps (Hymenoptera: Vespidae) in farming of organic tobacco (Nicotiana tabacum L.) in south of Brazil

This work aims to recognize and record the occurrence of species of social wasps (Polistinae) in organic tobacco crops, as well as to point out possible species that may be used in future biological control programs. The research was conducted in Virginia-type tobacco farming with organic management in two regions in south of Brazil. The collections were carried out with Malaise trap, during the harvests 2008/2009, 2009/2010 and 2010/2011. A total of 2.738 wasps, from which 23 species distributed in six genera was collected. Polistes was the most diverse genus (8 species), followed by Polybia, Mischocyttarus, Agelaia, Bachygastra and Protonectarina. Polybia scutellaris was the most abundant species, being considered eudominant. Agelaia multipicta and Polybia sericea with are also among the most abundant. This information is relevant for the insertion of social wasps with potential agents in the biological control of tobacco pests

Anaerobic biodegradation of an organic by-products leachate by interaction with different mine tailings

Abstract: Deinking by-products from paper recycling has been used as cover material on acid-producing tailings. Due to remaining cellulose, anaerobic degradation leads to the production of an organic-rich leachate, which may percolate through the tailings. This study aims at describing the influence of the tailings’ characteristics on the attenuation of organic matter in leachate and at discussing the degradation mechanisms. To this end, leachate was mixed with different types of tailings, includ ing three unoxidized tailings with varying acid generation potentials and one tailing in three states of oxidation. Regularly, selected biochemical parameters were analyzed to access the evolution of organic components. The results show that when deinking by-products leachate was placed in contact with tailings, phase and acid-base balance reactions took place in the beginning. Subsequently, oxidationreduction reactions dominated the chemistry of the system. The type, the constituents and the state of oxid ation of the mine tailings condition the mechanisms of biodegradation of organic components. Methanogenesis was predominant in the control sample (pure organic leachate) but was absent in all leachate-tailings mixes. No biodegradation was observed in the liquid phase for oxidized tailings and the organic concentration remained constant for unoxidized tailings, independently of the acid-generation potential. The biodegradation efficiency was optimal when the leachate was in contact with a mixture of oxidized and unoxidized tailings due to sulfate-reduction

SAMPLEX: Automatic mapping of perturbed and unperturbed regions of proteins and complexes

<p>Abstract</p> <p>Background</p> <p>The activity of proteins within the cell is characterized by their motions, flexibility, interactions or even the particularly intriguing case of partially unfolded states. In the last two cases, a part of the protein is affected either by binding or unfolding and the detection of the respective perturbed and unperturbed region(s) is a fundamental part of the structural characterization of these states. This can be achieved by comparing experimental data of the same protein in two different states (bound/unbound, folded/unfolded). For instance, measurements of chemical shift perturbations (CSPs) from NMR ¹H-¹⁵N HSQC experiments gives an excellent opportunity to discriminate both moieties.</p> <p>Results</p> <p>We describe an innovative, automatic and unbiased method to distinguish perturbed and unperturbed regions in a protein existing in two distinct states (folded/partially unfolded, bound/unbound). The SAMPLEX program takes as input a set of data and the corresponding three-dimensional structure and returns the confidence for each residue to be in a perturbed or unperturbed state. Its performance is demonstrated for different applications including the prediction of disordered regions in partially unfolded proteins and of interacting regions in protein complexes.</p> <p>Conclusions</p> <p>The proposed approach is suitable for partially unfolded states of proteins, local perturbations due to small ligands and protein-protein interfaces. The method is not restricted to NMR data, but is generic and can be applied to a wide variety of information.</p

Something fishy in the Great Lakes?:The reappraisal of early pottery use in north-eastern North America.

Lipid residue analysis has recently been applied to investigate the adoption of pottery by Early Woodland hunter-gatherers in north-eastern North America. Results, however, have proven contradictory, especially regarding the extent towhich early ceramics were used for processing aquatic resources. Here, the authors argue that this inconsistency is due to the use of different analytical procedures and criteria for identifying aquatic organisms, rather than anyactual variations in pottery use. By applying robust analytical criteria and methods to Early Woodland pottery from the Great Lakes region, the authors present evidence supporting their hypothesis that such pottery was indeed used for processing aquatic resource

Interaction of fluorescently labeled pyrrole-imidazole polyamide probes with fixed and living murine and human cells

International audiencePericentromeric heterochromatin plays important roles in controlling gene expression and cellular differentiation. Fluorescent pyrrole-imidazole polyamides targeting murine pericentromeric DNA (major satellites) can be used for the visualization of pericentromeric heterochromatin foci in live mouse cells. New derivatives targeting human repeated DNA sequences (a-satellites) were synthesized and their interaction with target DNA was characterized. The possibility to use major satellite and a-satellite binding polyamides as tools for staining pericentromeric heterochromatin was further investigated in fixed and living mouse and human cells. The staining that was previously observed using the mouse model was further characterized and optimized, but remained limited regarding the fluorophores that can be used. The promising results regarding the staining in the mouse model could not be extended to the human model. Experiments performed in human cells showed chromosomal DNA staining without selectivity. Factors limiting the use of fluorescent polyamides, in particular probe aggregation in the cytoplasm, were investigated. Results are discussed with regards to structure and affinity of probes, density of target sites and chromatin accessibility in both models

A produção cultural e literária no movimento estudantil paranaense na década de 1960

This text analyzes the cultural production carried out by the student movement of Paraná, both through the link to the national works of the Popular Culture Center and the national student movement, contributing to the history of education. The research methodology developed here combined the examination of the bibliography on the subject with the analysis of the extensive documentation produced by the Political and Social Order Department (DOPS), mainly in the DOPS of Paraná. The objective of this work is to identify the cultural production of students related to literary production, contextualizing it among the main objectives and flags inherent to the student movement.El artículo tiene como objetivo comprender las características del proceso de descentralización de la financiación de las políticas Este texto analiza la producción cultural llevada a cabo por el movimiento estudiantil de Paraná, tanto desde el enlace a las obras nacionales del Centro de Cultura Popular como al movimiento estudiantil nacional, contribuyendo con la historia de la educación. La metodología de investigación desarrollada aquí combinó el examen de la bibliografía sobre el tema con el análisis de la extensa documentación producida por las Delegacias o Departamentos de Ordem Política e Social (DOPS), principalmente en el DOPS de Paraná. El objetivo de este trabajo es identificar la producción cultural de estudiantes relacionada con la producción literaria, contextualizándola en medio de los principales objetivos y banderas inherentes al movimiento estudiantil.Este texto analisa a produção cultural levada a cabo pelo movimento estudantil paranaense, tanto por meio do vínculo aos trabalhos nacionais do Centro Popular de Cultura como pelo movimento estudantil nacional, contribuindo com a história da educação. A metodologia da pesquisa aqui desenvolvida aliou o exame da bibliografia sobre o tema à análise da extensa documentação produzida pelas Delegacias ou Departamento de Ordem Política e Social (DOPS), principalmente, na DOPS do Paraná. Objetiva-se, ainda, neste trabalho, identificar a produção cultural dos estudantes, que se relaciona à produção literária, contextualizando-a em meio aos principais objetivos e bandeiras inerentes ao movimento estudantil