Plasticizer degradation by marine bacterial isolates : a proteogenomic and metabolomic characterization

Many commercial plasticizers are toxic endocrine-disrupting chemicals that are added to plastics during manufacturing and may leach out once they reach the environment. Traditional phthalic acid ester plasticizers (PAEs), such as dibutyl phthalate (DBP) and bis(2-ethyl hexyl) phthalate (DEHP), are now increasingly being replaced with more environmentally friendly alternatives, such as acetyl tributyl citrate (ATBC). While the metabolic pathways for PAE degradation have been established in the terrestrial environment, to our knowledge, the mechanisms for ATBC biodegradation have not been identified previously and plasticizer degradation in the marine environment remains underexplored. From marine plastic debris, we enriched and isolated microbes able to grow using a range of plasticizers and, for the first time, identified the pathways used by two phylogenetically distinct bacteria to degrade three different plasticizers (i.e., DBP, DEHP, and ATBC) via a comprehensive proteogenomic and metabolomic approach. This integrated multi-OMIC study also revealed the different mechanisms used for ester side-chain removal from the different plasticizers (esterases and enzymes involved in the β-oxidation pathway) as well as the molecular response to deal with toxic intermediates, that is, phthalate, and the lower biodegrading potential detected for ATBC than for PAE plasticizers. This study highlights the metabolic potential that exists in the biofilms that colonize plastics-the Plastisphere-to effectively biodegrade plastic additives and flags the inherent importance of microbes in reducing plastic toxicity in the environment

Exploring gravity wave characteristics in 3-D using a novel S-transform technique: AIRS/Aqua measurements over the Southern Andes and Drake Passage

Gravity waves (GWs) transport momentum and energy in the atmosphere, exerting a profound influence on the global circulation. Accurately measuring them is thus vital both for understanding the atmosphere and for developing the next generation of weather forecasting and climate prediction models. However, it has proven very difficult to measure the full set of GW parameters from satellite measurements, which are the only suitable observations with global coverage. This is particularly critical at latitudes close to 60° S, where climate models significantly under-represent wave momentum fluxes. Here, we present a novel fully 3-D method for detecting and characterising GWs in the stratosphere. This method is based around a 3-D Stockwell transform, and can be applied retrospectively to existing observed data. This is the first scientific use of this spectral analysis technique. We apply our method to high-resolution 3-D atmospheric temperature data from AIRS/Aqua over the altitude range 20–60 km. Our method allows us to determine a wide range of parameters for each wave detected. These include amplitude, propagation direction, horizontal/vertical wavelength, height/direction-resolved momentum fluxes (MFs), and phase and group velocity vectors. The latter three have not previously been measured from an individual satellite instrument. We demonstrate this method over the region around the Southern Andes and Antarctic Peninsula, the largest known sources of GW MFs near the 60° S belt. Our analyses reveal the presence of strongly intermittent highly directionally focused GWs with very high momentum fluxes (∼ 80–100 mPa or more at 30 km altitude). These waves are closely associated with the mountains rather than the open ocean of the Drake Passage. Measured fluxes are directed orthogonal to both mountain ranges, consistent with an orographic source mechanism, and are largest in winter. Further, our measurements of wave group velocity vectors show clear observational evidence that these waves are strongly focused into the polar night wind jet, and thus may contribute significantly to the "missing momentum" at these latitudes. These results demonstrate the capabilities of our new method, which provides a powerful tool for delivering the observations required for the next generation of weather and climate models

Transgenic mice expressing mutant forms VCP/p97 recapitulate the full spectrum of IBMPFD including degeneration in muscle, brain and bone

Inclusion body myopathy associated with Paget's disease of bone and frontotemporal dementia (IBMPFD) is a dominantly inherited degenerative disorder caused by mutations in the valosin-containing protein (VCP) gene. VCP (p97 in mouse, TER94 in Drosophila melanogaster and CDC48 in Saccharomyces cerevisiae) is a highly conserved AAA+-ATPase that regulates a wide array of cellular processes. The mechanism of IBMPFD pathogenesis is unknown. Towards elucidating the pathogenic mechanism we have developed and characterized transgenic mice with ubiquitous expression of wild-type and disease-causing versions of human VCP/p97. Here, we report that mice expressing VCP/p97 harboring the mutations R155H or A232E develop pathology that is limited to muscle, brain and bone, recapitulating the spectrum of disease in humans with IBMPFD. The mice exhibit progressive muscle weakness and pathological examination of muscle shows classic characteristics of inclusion body myopathy including rimmed vacuoles and TDP-43 pathology. The mice exhibit abnormalities in behavioral testing and pathological examination of the brain shows widespread TDP-43 pathology. Furthermore, radiological examination of the skeleton reveals that mutant mice develop severe osteopenia accompanied by focal lytic and sclerotic lesions in vertebrae and femur. In vitro studies indicate that mutant VCP causes inappropriate activation of the NF-κB signaling cascade, which could contribute to the mechanism of pathogenesis in multiple tissues including muscle, bone and brai

"Be active!" Revisiting the South African Food Based Dietary Guidelines for activity

The objective of this paper was to review current evidence on physical activity for health in order to support the foodbased dietary guideline (FBDG) “Be active!”. Physical activity, defined as at least 30 minutes of moderate-intensity physical activity per day for adults, and 60 minutes for children and adolescents, is advised in the FBDG because of the role it plays in maintaining energy balance, improving body composition and promoting general health and wellbeing. The reviewed outcome measures are changes in physical activity patterns and the reported prevalence of noncommunicable diseases (NCDs) in South Africa. Despite the previous set of FBDGs, no improvements in physical activity, obesity or NCDs have been reported in South Africa. Recent literature emphasises the beneficial effects of physical activity on the reduction of risk factors associated with the prevalence of NCDs. Physical activity has a positive effect on appetite and weight control, insulin sensitivity, dyslipidaemia, hypertension, stress relief and burnout. Barriers that prevent children and adults from participating in regular physical activity have been identified, and recommendations how to overcome these have been made. It has been concluded that South Africans are not sufficiently physically active for their general health status to be improved. It is recommended that methods to promote physical activity at national, provincial, district and local level need to be developed, implemented and sustained

Black Hole Masses and Star Formation Rates of z >1 Dust Obscured Galaxies (DOGs): Results from Keck OSIRIS Integral Field Spectroscopy

We have obtained high spatial resolution Keck OSIRIS integral field spectroscopy of four z~1.5 ultra-luminous infrared galaxies that exhibit broad H-alpha emission lines indicative of strong AGN activity. The observations were made with the Keck laser guide star adaptive optics system giving a spatial resolution of 0.1", or <1 kpc at these redshifts. These high spatial resolution observations help to spatially separate the extended narrow-line regions --- possibly powered by star formation --- from the nuclear regions, which may be powered by both star formation and AGN activity. There is no evidence for extended, rotating gas disks in these four galaxies. Assuming dust correction factors as high as A(H-alpha)=4.8 mag, the observations suggest lower limits on the black hole masses of (1 - 9) x 10^8 solar masses, and star formation rates <100 solar masses per year. The black hole masses and star formation rates of the sample galaxies appear low in comparison to other high-z galaxies with similar host luminosities. We explore possible explanations for these observations including, host galaxy fading, black hole growth, and the shut down of star formation.Comment: Accepted for publication in the Astronomical Journal. 12 pages, 6 figures, 5 table

Highly selective CO2 vs. N2 adsorption in the cavity of a molecular coordination cage

Two M8L12 cubic coordination cages, as desolvated crystalline powders, preferentially adsorb CO2 over N2 with ideal selectivity CO2/N2 constants of 49 and 30 at 298 K. A binding site for CO2 is suggested by crystallographic location of CS2 within the cage cavity at an electropositive hydrogen-bond donor site, potentially explaining the high CO2/N2 selectivity compared to other materials with this level of porosity