The Basic Law at 60 - Introduction to the Special Issue

For Germany 2009 was a year of constitutional anniversaries: the first democratic constitution (Paulskirchenverfassung of 1849) was promulgated 160 years ago; the 1919 Weimar Constitution would have turned 90; and finally, the country celebrated 60 years of the Basic Law, which was proclaimed and signed in Bonn on 23 May 1949. Despite its birth in the midst of economic and political turmoil and widespread disillusion with politics, the Basic Law has come to be regarded as a success story. As is well known, it was never meant to last - the very term Grundgesetz (basic law) indicated that it was intended to serve as a temporary constitutional framework until the enactment of a new constitution for the whole of Germany. Yet the Basic Law outgrew its provisional character. Today, not only the political establishment is united in praising the Grundgesetz. The scholarly assessment also has been mostly positive. The constitutional bargain struck in 1949 has been able to achieve what no previous German constitution had managed. The right and the left of the German political spectrum fashioned an enduring compromise that combined democracy, federalism and the welfare state. It is part of the story that the old anti-liberal and nationalist elite had been thoroughly delegitimized by loosing the war. Also, the Allies gave the effort an additional nudge. The progressive changes could then be implemented quite effectively by relying on the juristic culture of the Rechtsstat that dates back to the bureaucratic legacy of, among others, the Prussian state

Comprehensive Security Research to Contribute to Critical Infrastructure Protection Contributions to Security Governance in Disaster Risk Reduction

Critical infrastructure protection (CIP) has become a major issue in civil security, emergency management and natural hazard management. The all-hazard approach has gained ground on the international scale, and the “comprehensive approach” in security policies and security research has been advanced in order to meet current and future threats based on better integrated information, assessment, policies and capabilities. This paper aims to showcase this “comprehensive approach”, highlighting its character and cross-links to CI and natural hazard and disaster management. The paper also contributes to a broader perspective on CIP by addressing current European political concepts and socio-cultural conditions, as well as possible future EU roles. A focus is put on international critical infrastructure (CI) risks, and results from an Integrated Risk Taxonomy are presented. The paper concludes with proposing socio-cultural aspects for future research topics related to CI risks and security governance

Preservation of nautilid soft parts inside and outside the conch interpreted as central nervous system, eyes, and renal concrements from the Lebanese Cenomanian

Nautilid, coleoid and ammonite cephalopods preserving jaws and soft tissue remains are moderately common in the extremely fossiliferous Konservat-Lagerstätte of the Hadjoula, Haqel and Sahel Aalma region, Lebanon. We assume that hundreds of cephalopod fossils from this region with soft-tissues lie in collections worldwide. Here, we describe two specimens of Syrionautilus libanoticus (Cymatoceratidae, Nautilida, Cephalopoda) from the Cenomanian of Hadjoula. Both specimens preserve soft parts, but only one shows an imprint of the conch. The specimen without conch displays a lot of anatomical detail. We homologise the fossilised structures as remains of the digestive tract, the central nervous system, the eyes, and the mantle. Small phosphatic structures in the middle of the body chamber of the specimen with conch are tentatively interpreted as renal concrements (uroliths). The absence of any trace of arms and the hood of the specimen lacking its conch is tentatively interpreted as an indication that this is another leftover fall (pabulite), where a predator lost parts of its prey. Other interpretations such as incomplete scavenging are also conceivable

UAS Literary & Arts Journal

Proof copy provided by Tidal Echoes.The 2016 edition of Tidal Echoes presents an annual showcase of writers and artists who share one thing in common: a life surrounded by the rainforests and waterways of Southeast Alaska.a heart is a heavy burden -- Editor’s Note -- Acknowledgements -- Always a Pause -- Untitled -- Mishima Teabowls -- Mint Tea and Red Wine -- The Dry Winter Scent of Prairies -- Northern Sunrise -- Musical Theory in a Falling Tree -- Jumbo Lookout -- The Shape of an Echo -- Wonderwall -- It’s Difficult [excerpt from the poem “Marissa”] -- Yéil x’us.eetí Raven Footprints -- táakw winter -- King Fisher -- Bear -- A Dandelion by its own Name -- Migrations -- Untitled -- They Named Her Driftwood -- Kissing in the Rain -- Waltz of the Flowers: Anna and Company -- Sun Catcher -- Porcelain Curtains -- The River -- Kathleen Lake -- A Living Tapestry -- Red Cedar Tlingit Haida Weave -- Skinned -- Survivor -- Auke Lake Lights -- Aurora Chasers -- Emma Afloat -- I Can’t Sleep -- The Ghost I’m Left With -- Framed Memories -- Tequila, Sweat, and Prayers -- The Alchemical Marriage -- A New New Hope -- each dream practice -- Clouds -- Seasons Change and the Waters Run On -- John Muir -- Salmon Speaks -- A Place That Holds Names -- Untitled -- Salmon Spirit Chest, Connected (box) -- The Language of Weaving: Featured Artist Dr. Teri Rofkar -- Nome, Alaska -- Drained -- I’m From -- Srevlla—The state of things where the spring snow is so soft that one sinks into it. -- Reflections -- Txamsem -- Post-Glacial Rebound -- Dressed in Garlands -- Learning the Dance -- Shamrocks -- When You See Me -- Water Spirit -- To Speak for Restraint, for Wildness, for Beauty: Featured Writer Aleria Jensen -- Young Eagle -- Untitled (detail) -- Baula -- Airport Dike Trail Evening -- Ninja Meatballs -- Untitled -- In the Wild Without Child: One Mother’s Invitation to Self -- Whale Tail Vista -- Questions for Anemones -- Yellow Cedarbark Wool on Starfish Pot -- Gleaming Orange and Pissed -- Through the Sky She Comes -- Love for the Honeybee -- The Last Speaker -- Juneau Fireweed -- Judy Plays the Tuba -- Illuminated Juneau -- Airport Dike Trail Moonlight -- Escape -- A Wooden Mother -- Black and Whites -- an excuse for staying indoors (a working title for a work in progress) -- Insecurities -- Lake Farm -- Yew Bear -- Rupture -- Mendenhall Glacier -- The Rookery -- Transient -- Laminaria -- Untitled -- Querencia -- Hoard -- Untitled -- Small Birds Sign -- Selfie at Two -- Entropy -- Goodbye -- Sitka Bridge -- My Remedy -- S1 -- Delta Symbols Static -- Falling -- Breathe Deeply -- Drum, drum away -- Eaglefest Dancer -- Wasichana -- Writer & Artist Biographie

Filaggrin-stratified transcriptomic analysis of pediatric skin identifies mechanistic pathways in patients with atopic dermatitis

BackgroundAtopic dermatitis (AD; eczema) is characterized by a widespread abnormality in cutaneous barrier function and propensity to inflammation. Filaggrin is a multifunctional protein and plays a key role in skin barrier formation. Loss-of-function mutations in the gene encoding filaggrin (FLG) are a highly significant risk factor for atopic disease, but the molecular mechanisms leading to dermatitis remain unclear.ObjectiveWe sought to interrogate tissue-specific variations in the expressed genome in the skin of children with AD and to investigate underlying pathomechanisms in atopic skin.MethodsWe applied single-molecule direct RNA sequencing to analyze the whole transcriptome using minimal tissue samples. Uninvolved skin biopsy specimens from 26 pediatric patients with AD were compared with site-matched samples from 10 nonatopic teenage control subjects. Cases and control subjects were screened for FLG genotype to stratify the data set.ResultsTwo thousand four hundred thirty differentially expressed genes (false discovery rate, P < .05) were identified, of which 211 were significantly upregulated and 490 downregulated by greater than 2-fold. Gene ontology terms for “extracellular space” and “defense response” were enriched, whereas “lipid metabolic processes” were downregulated. The subset of FLG wild-type cases showed dysregulation of genes involved with lipid metabolism, whereas filaggrin haploinsufficiency affected global gene expression and was characterized by a type 1 interferon–mediated stress response.ConclusionThese analyses demonstrate the importance of extracellular space and lipid metabolism in atopic skin pathology independent of FLG genotype, whereas an aberrant defense response is seen in subjects with FLG mutations. Genotype stratification of the large data set has facilitated functional interpretation and might guide future therapy development

Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies

Anti-glycan antibodies represent a vast and yet insufficiently investigated subpopulation of naturally occurring and adaptive antibodies in humans. Recently, a variety of glycan-based microarrays emerged, allowing high-throughput profiling of a large repertoire of antibodies. As there are no direct approaches for comparison and evaluation of multi-glycan assays we compared three glycan-based immunoassays, namely printed glycan array (PGA), fluorescent microsphere-based suspension array (SA) and ELISA for their efficacy and selectivity in profiling anti-glycan antibodies in a cohort of 48 patients with and without ovarian cancer. The ABO blood group glycan antigens were selected as well recognized ligands for sensitivity and specificity assessments. As another ligand we selected P1, a member of the P blood group system recently identified by PGA as a potential ovarian cancer biomarker. All three glyco-immunoassays reflected the known ABO blood groups with high performance. In contrast, anti-P1 antibody binding profiles displayed much lower concordance. Whilst anti-P1 antibody levels between benign controls and ovarian cancer patients were significantly discriminated using PGA (p = 0.004), we got only similar results using SA (p = 0.03) but not for ELISA. Our findings demonstrate that whilst assays were largely positively correlated, each presents unique characteristic features and should be validated by an independent patient cohort rather than another array technique. The variety between methods presumably reflects the differences in glycan presentation and the antigen/antibody ratio, assay conditions and detection technique. This indicates that the glycan-antibody interaction of interest has to guide the assay selectio

“What if There's Something Wrong with Her?”‐How Biomedical Technologies Contribute to Epistemic Injustice in Healthcare

While there is a steadily growing literature on epistemic injustice in healthcare, there are few discussions of the role that biomedical technologies play in harming patients in their capacity as knowers. Through an analysis of newborn and pediatric genetic and genomic sequencing technologies (GSTs), I argue that biomedical technologies can lead to epistemic injustice through two primary pathways: epistemic capture and value partitioning. I close by discussing the larger ethical and political context of critical analyses of GSTs and their broader implications for just and equitable healthcare delivery

DNA damage alters nuclear mechanics through chromatin reorganization

AbstractDNA double-strand breaks drive genomic instability. However, it remains unknown how these processes may affect the biomechanical properties of the nucleus and what role nuclear mechanics play in DNA damage and repair efficiency. Here, we have used Atomic Force Microscopy to investigate nuclear mechanical changes, arising from externally induced DNA damage. We found that nuclear stiffness is significantly reduced after cisplatin treatment, as a consequence of DNA damage signalling. This softening was linked to global chromatin decondensation, which improves molecular diffusion within the organelle. We propose that this can increase recruitment for repair factors. Interestingly, we also found that reduction of nuclear tension, through cytoskeletal relaxation, has a protective role to the cell and reduces accumulation of DNA damage. Overall, these changes protect against further genomic instability and promote DNA repair. We propose that these processes may underpin the development of drug resistance

Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies

Anti-glycan antibodies represent a vast and yet insufficiently investigated subpopulation of naturally occurring and adaptive antibodies in humans. Recently, a variety of glycan-based microarrays emerged, allowing high-throughput profiling of a large repertoire of antibodies. As there are no direct approaches for comparison and evaluation of multi-glycan assays we compared three glycan-based immunoassays, namely printed glycan array (PGA), fluorescent microsphere-based suspension array (SA) and ELISA for their efficacy and selectivity in profiling anti-glycan antibodies in a cohort of 48 patients with and without ovarian cancer. The ABO blood group glycan antigens were selected as well recognized ligands for sensitivity and specificity assessments. As another ligand we selected P1, a member of the P blood group system recently identified by PGA as a potential ovarian cancer biomarker. All three glyco-immunoassays reflected the known ABO blood groups with high performance. In contrast, anti-P1 antibody binding profiles displayed much lower concordance. Whilst anti-P1 antibody levels between benign controls and ovarian cancer patients were significantly discriminated using PGA (p = 0.004), we got only similar results using SA (p = 0.03) but not for ELISA. Our findings demonstrate that whilst assays were largely positively correlated, each presents unique characteristic features and should be validated by an independent patient cohort rather than another array technique. The variety between methods presumably reflects the differences in glycan presentation and the antigen/antibody ratio, assay conditions and detection technique. This indicates that the glycan-antibody interaction of interest has to guide the assay selection

Considering weed management as a social dilemma bridges individual and collective interests

Weeds pose severe threats to agricultural and natural landscapes worldwide. One major reason for the failure to effectively manage weeds at landscape scales is that current Best Management Practice guidelines, and research on how to improve such guidelines, focus too narrowly on property-level management decisions. Insufficiently considered are the aggregate effects of individual actions to determine landscape-scale outcomes, or whether there are collective practices that would improve weed management outcomes. Here, we frame landscape-scale weed management as a social dilemma, where trade-offs occur between individual and collective interests. We apply a transdisciplinary system approach—integrating the perspectives of ecologists, evolutionary biologists and agronomists into a social science theory of social dilemmas—to four landscape-scale weed management challenges: (i) achieving plant biosecurity, (ii) preventing weed seed contamination, (iii) maintaining herbicide susceptibility and (iv) sustainably using biological control. We describe how these four challenges exhibit characteristics of ‘public good problems’, wherein effective weed management requires the active contributions of multiple actors, while benefits are not restricted to these contributors. Adequate solutions to address these public good challenges often involve a subset of the eight design principles developed by Elinor Ostrom for ‘common pool social dilemmas’, together with design principles that reflect the public good nature of the problems. This paper is a call to action for scholars and practitioners to broaden our conceptualization and approaches to weed management problems. Such progress begins by evaluating the public good characteristics of specific weed management challenges and applying context-specific design principles to realize successful and sustainable weed management