Evaluation of a Multiplex PCR Assay for the Identification of Campylobacter jejuni and Campylobacter coli

Background: Campylobacter jejuni and Campylobacter coli are identified as the major causes of acute gastroenteritis in humans. Because of the fastidious nature of Campylobacters, many clinical laboratories fail to routinely culture them. The detection of Campylobacter spp. using molecular-based techniques can be useful for diagnostic and epidemiological applications. This study aimed to develop a multiplex PCR assay for the simultaneous detection of C. jejuni and C. coli strains from clinical specimens. Materials and Methods: During a 19-month period, stool samples were collected from 980 children admitted to a hospital in Tehran, Iran and then examined. The samples were cultured on both Brucella agar and Modified Charcoal-Cefoperazone-Deoxycholate agar (mCCDA) media at 42\ub0C for 48 h. To confirm suspected bacteria, Gram staining and other biochemical tests were carried out. Finally, after extracting DNA from pure cultures using the boiling method, the multiplex PCR assay was performed. Results: The multiplex PCR assay showed that Campylobacter spp. can be detected using 400 bp target product of cadF. It can also accurately distinguish between C. jejuni and C. coli species with different bands of 735 bp and 500 bp using hipO and asp genes, respectively. Conclusions: Results showed that the multiplex PCR assay can replace the biochemical assays for differentiating between C. jejuni and C. coli strains in a single-step PCR test

Can Greater Flamingo Recognize Fertile <i>vs.</i> Infertile Egg? A Single Case Study

Fertility of captive flamingos varies between flocks, species and seasons. Individuating infertile eggs could be helpful to facilitate important decisions. Wild animals could be encouraged to abandon the nest or not, whereas in captivity removing non-viable egg would lead birds to lay a new one. The aim of this study was to investigate the parental behaviour of a pair of greater flamingos (Phoenicopterus roseus ) in the presence of a fertile and an infertile egg. Data on the posture and behaviours of the pair on the nest were collected over two different periods: first period\u2014an infertile egg was laid; second period\u2014a fertile egg was laid. For each period, 28 ten-minute sessions per flamingo partner were run. Results revealed that female flamingo spent significantly more time standing on the nest in the first than in the second period (P = 0.010). Moreover, when standing on the nest, the female performed significantly more egg-care behaviour (attention to the egg, egg rotation/moving) in the first than in the second period (P = 0.010). No significant differences between periods emerged in the male flamingo posture on the nest and behaviours. Findings from this study suggest that female flamingos stand on the nest longer if the egg is infertile, paying more attention and examining it deeply. This study provides new insights into greater flamingo parent-embryo communication. Future research is needed to improve our knowledge on this topic, as well as on the husbandry of this species in the controlled environment

How to be a great dad: Parental care in a flock of greater flamingo (Phoenicopterus roseus)

In the last years, studies on captive greater flamingos have increased. Research on zoo animals is important to improve the knowledge on these species and to improve their ex-situ and in-situ conservation. The aim of the present study was to investigate the parental behaviour of a captive colony of greater flamingo hosted at Parco Natura Viva, an Italian zoological garden, to improve the knowledge on this species in zoos. In particular, the present study investigated and compared the parental care of females and males in 35 breeding pairs of greater flamingos. For each pair, we collected durations of parental care behaviour of both females and males, recording their position in relation to the nest (near the nest, on the nest, away from the nest) and individual and social behaviours performed. First, both partners were involved in parental care and displayed species-specific behaviours reported in the wild. The main results were that males spent more time than females on the nest (P = 0.010) and near it (P = 0.0001) and were more aggressive toward other flamingos than females, both when sitting on the nest (P = 0.003) and when near the nest (P = 0.0003). Therefore, male flamingos seem to be more involved in incubation duties and nest protection than females. This kind of research is important not only to expand the knowledge on bird species such as flamingos, but also to improve their husbandry and breeding in controlled environment. Indeed, understanding animal behaviour allows us to gain insights into their individual and social needs, addressing potential animal welfare issues

Case ReportCeruminous otitis in native chicken breeders belonging toRobusta Lionatabreed

At the beginning of 2005, an outbreak of ceruminous otitis has been observed in a breeders flock belonging to the Robusta Lionata breed, a native chicken breed reared in a Centre for the valorisation and conservation of native poultry breeds. The disease caused a high morbidity rate (90% of birds), whereas the mortality affected only 10% of the birds. The death of the birds was preceded by clinical signs, such as worsening of the ceruminous otitis with abundant secretion of bad-smelling cerumen, lack of appetite and depression. The otitis externa, mainly bilateral, was the only evident finding at necropsy. Laboratory examinations showed only the presence of Mycoplasma synoviae, either by isolation on culture media or by PCR, on ear and choana samples. Aspergillus fumigatus was detected only in one bird. In this paper, the Authors report for the first time on an outbreak of ceruminous otitis in native chicken breeds and point out that the only pathogenic agent isolated from the birds has been Mycoplasma synoviae

Multilocus Sequence Typing of Commensal and EnteropathogenicEscherichia Colifrom Domestic and Wild Lagomorphs in Italy

The aim of the study was to determine the multilocus sequence types of Escherichia coli from diseased farm rabbits and apparently healthy wild lagomorphs, and the genetic relatedness among them. Fifty-five enteropathogenic E. coli from reared rabbits and 32 from wild rabbits and hares were characterised by multilocus sequence typing (MLST) according to the Michigan State University EcMLST scheme. Isolates were differentiated into 37 sequence types (STs), which were grouped into 8 clonal complexes (CCs). The most common ST was ST140 (CC31), followed by ST238 and ST119 (CC17). MLST analysis revealed 22 novel STs. Phylogenetic analyses showed a heterogeneous distribution of STs into 3 clusters of genetically related strains. The genetic relationship among STs of different origin and the detection of new, as well as previously described STs as human pathogens, indicate a widespread distribution and adaptability of particular lineages to different hosts. These findings highlight the need for further research to improve the knowledge about E. coli populations colonising the gut of lagomorphs and their zoonotic potential

Full genome sequence-based comparative study of wild-type and vaccine strains of infectious laryngotracheitis virus from Italy

Infectious laryngotracheitis (ILT) is an acute and highly contagious respiratory disease of chickens caused by an alphaherpesvirus, infectious laryngotracheitis virus (ILTV). Recently, full genome sequences of wild-type and vaccine strains have been determined worldwide, but none was from Europe. The aim of this study was to determine and analyse the complete genome sequences of five ILTV strains. Sequences were also compared to reveal the similarity of strains across time and to discriminate between wild-type and vaccine strains. Genomes of three ILTV field isolates from outbreaks occurred in Italy in 1980,2007 and 2011, and two commercial chicken embryo origin (CEO) vaccines were sequenced using the 454 Life Sciences technology. The comparison with the Serva genome showed that 35 open reading frames (ORFs) differed across the five genomes. Overall, 54 single nucleotide polymorphisms (SNPs) and 27 amino acid differences in 19 ORFs and two insertions in the UL52 and ORFC genes were identified. Similarity among the field strains and between the field and the vaccine strains ranged from 99.96% to 99.99%. Phylogenetic analysis revealed a close relationship among them, as well. This study generated data on genomic variation among Italian ILTV strains revealing that, even though the genetic variability of the genome is well conserved across time and between wild-type and vaccine strains, some mutations may help in differentiating among them and maybe involved in ILTV virulence/attenuation. The results of this study can contribute to the understanding of the molecular bases of ILTV pathogenicity and provide genetic markers to differentiate between wild-type and vaccine strains

Cork-derived hierarchically porous hydroxyapatite with different stoichiometries for biomedical and environmental applications

Hierarchically porous hydroxyapatite derived from cork powder shows excellent performance in biomedicine (low cytotoxicity) and environmental remediation (high Pb2+ removal)

Poly(l-lactide-co-caprolactone-co-glycolide)-based nanoparticles as delivery platform: effect of the surfactants on characteristics and delivery efficiency

Polymeric nanoparticles made of the copolymer Poly(L-lactide-co-caprolactone-co-glycolide) were prepared using the solvent evaporation method. Two different surfactants, polyvinyl alcohol and dextran, and a mixture of the two were employed. The three types of nanoparticles were used as hosting carriers of two chemotherapeutic drugs, the hydrophilic doxorubicin and the hydrophobic SN-38. The morphostructural characterization showed similar features for the three types of nanoparticles, while the drug encapsulation efficiency indicated that the dextran-based systems are the most effective with both drugs. Cellular studies with breast cancer cells were performed to compare the delivery capability and the cytotoxicity profile of the three nanosystems. The results show that the unloaded nanoparticles are highly biocompatible at the administered concentrations and confirmed that dextran-coated nanoparticles are the most efficient vectors to release the two drugs, exerting cytotoxic activity. PVA, on the other hand, shows limited drug release in vitro, probably due to strong interactions with both drugs. Data also show the release is more efficient for doxorubicin than for SN-38; indeed, the doxorubicin IC50 value for the dextran-coated nanoparticles was about 35% lower than the free drug. This indicates that these nanocarriers are suitable candidates to deliver hydrophilic drugs while needing further modification to host hydrophobic molecules.info:eu-repo/semantics/publishedVersio

Effect of genotype, gender and feed restriction on growth, meat quality and the occurrence of white striping and wooden breast in broiler chickens

Due to their importance for the control of meat quality in broiler chickens, the present study aimed at identifying the factors associated with the occurrence of myopathies and characterizing the meat properties when affected by myopathies. To this aim, a total of 768 broiler chickens were reared until slaughter (46 d) to evaluate the effect of genotype, gender, and feeding regime (ad libitum vs. restricted rate, 80% from 13 to 21 d of age) on performance and meat quality. Standard broilers were heavier (3,270 vs. 3,139 g; P < 0.001) and showed lower feed conversion (1.56 vs. 1.61; P < 0.001) than the high-yield broilers. Males showed higher final live weight (3,492 vs. 2,845 g) and lower feed conversion (1.54 vs. 1.63) than females (P < 0.001). Feed restriction decreased final live weight (3,194 vs. 3,142 g; P < 0.01) and feed conversion (1.60 vs. 1.57; P < 0.01) compared to ad libitum feeding. At gross examination, feed restriction tended to increase white-striped breasts (69.5 vs. 79.5%; P < 0.10), whereas females showed less wooden breasts than males (8.0 vs. 16.3%; P < 0.05). White-striped fillets had higher pHu (5.87 vs. 5.83), and lower a 17 ( 120.81 vs. 120.59) and b 17 color indexes (13.7 vs. 14.5) (P < 0.05), whereas wooden breast fillets exhibited higher cooking losses (25.6 vs. 22.1%) and AK-shear force (4.23 vs. 2.84 kg/g) compared with normal fillets (P < 0.001). At histological examination, 3.1% of pectoralis major were normal, 26.6% mildly degenerated, 45.3% moderately degenerated, and 25.0% severely degenerated. In conclusion, genotype had a moderate effect on growth without modifying myopathy occurrence. In contrast, gender and feed restriction affected performance, meat quality, and breast abnormalities

class 1 and class 2 integrons in avian pathogenic escherichia coli from poultry in italy

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