Health rather than excellence: the suffering of nutrition attendants of a Public Hospital affected by RSI/WRMSD

This article presents the results of a research that aimed to understand the relationship between aspects of the work organization and the health of nutrition workers affected by RSI/WRMSD. Throughout the process, absenteeism statistics related to RSI/WRMSD were obtained from a Public Hospital in Porto Alegre/RS, from 06/01/2015 to 06/30/2016, and a section of such hospital was selected for further study. We selected the Food Distribution Section and its nutrition attendants for the research. A structured questionnaire with sociodemographic and professional data and the Self-Reporting Questionnaire (SRQ-20) were used to investigate the prevalence of common mental disorders (CMD), and collective interviews were conducted with attendants affected by RSI/WRMSD, using the theoretical reference of the Work Psychodynamics. Results obtained pointed to ways of organizing and managing work which included excessive pressure, moral harassment, and lack of recognition, generating suffering and influencing the processes of illness. A space for listening and discussing work issues is suggested, with meetings involving all workers, nutrition technicians, and the coordination, which may characterize a permanent action.Univ Fed Sao Paulo, Dept Gestao & Cuidados Saude, Santos, SP, BrazilUniv Fed Rio Grande do Sul, Dept Med Social, Porto Alegre, RS, BrazilUniv Fed Sao Paulo, Dept Gestao & Cuidados Saude, Santos, SP, BrazilWeb of Scienc

Health rather than excellence : the suffering of nutrition attendants of a Public Hospital affected by RSI/WRMSD

Este artigo apresenta resultados de pesquisa cujo objetivo foi compreender as relações entre os aspectos da organização do trabalho e a saúde de atendentes de nutrição acometidos por LER/ Dort. Durante o processo houve levantamento de estatísticas de absenteísmo relacionado a LER/ Dort em um hospital público em Porto Alegre/RS, no período de 1 de junho de 2015 a 30 de junho de 2016, e seleção da Seção de Distribuição de Alimentos e de seus atendentes de nutrição para aprofundamentos. Foram utilizados um questionário estruturado com dados sociodemográficos e de trabalho e o instrumento Self-Reporting Questionnaire (SRQ20), para pesquisar a prevalência de transtornos mentais comuns (TMC), e realizadas entrevistas coletivas com os atendentes acometidos por LER/Dort, utilizando o referencial teórico da psicodinâmica do trabalho. Os resultados obtidos apontaram para modos de organização do trabalho e gestão com cobranças excessivas, assédio moral e falta de reconhecimento no trabalho, que geram sofrimento e influenciam nos processos de adoecimento. Sugere-se um espaço de escuta e discussões com reuniões que envolvam os trabalhadores, técnicos de nutrição e a coordenação que possam caracterizar uma ação permanente.This article presents the results of a research that aimed to understand the relationship between aspects of the work organization and the health of nutrition workers affected by RSI/WRMSD. Throughout the process, absenteeism statistics related to RSI/WRMSD were obtained from a Public Hospital in Porto Alegre/ RS, from 06/01/2015 to 06/30/2016, and a section of such hospital was selected for further study. We selected the Food Distribution Section and its nutrition attendants for the research. A structured questionnaire with sociodemographic and professional data and the Self-Reporting Questionnaire (SRQ-20) were used to investigate the prevalence of common mental disorders (CMD), and collective interviews were conducted with attendants affected by RSI/WRMSD, using the theoretical reference of the Work Psychodynamics. Results obtained pointed to ways of organizing and managing work which included excessive pressure, moral harassment, and lack of recognition, generating suffering and influencing the processes of illness. A space for listening and discussing work issues is suggested, with meetings involving all workers, nutrition technicians, and the coordination, which may characterize a permanent action

Membranas termosensibles basadas en redes poliméricas semi interpenetrantes de Quitosana y Poli (N-isopropilacrilamida)

O presente trabalho visa desenvolver membranas termossensíveis com um mecanismo inteligente de adesão/liberação e potente ação antimicrobiana para o tratamento de feridas. As membranas foram preparadas através da eletrossíntese do hidrogel termossensível poli(N-isopropilacrilamida) (PNIPAm) na presença da quitosana (QUI). O material final constitui uma rede polimérica semi-interpenetrante (sIPN) de QUI e PNIPAm. A quitosana é um biopolímero natural que possui ação bactericida, anti-inflamatória e cicatrizante. A quitosana comercial utilizada foi previamente caracterizada em termos de sua massa molar média (0,9312 * 105 g mol-1) por método viscosimétrico e grau de desacetilação (86,23%), através de titulação condutimétrica. O hidrogel PNIPAm foi incorporado à cadeia polimérica da QUI por via eletroquímica através da técnica de voltametria cíclica. A membrana sIPN QUI-PNIPAm obtida foi caracterizada por Espectroscopia no infravermelho por transformada de Fourier usando o modulo de Refletância Total Atenuada (FTIR-ATR), calorimetria exploratória diferencial (DSC) e termogravimétrica (TG). O espectro FTIR-ATR confirmou a polimerização do PNIPAm na presença da QUI. A curva TG mostrou que a membrana sIPN obtida apresenta uma composição de 33% de quitosana e 55% de PNIPAm. A análise térmica por DSC mostrou que a Tg da membrana sIPN QUI-PNIPAm é mais baixa que a Tg do hidrogel PNIPAm. A temperatura de transição de fase (LCST) da membrana sIPN QUI-PNIPAm foi determinada por espectroscopia na região do ultravioleta visível (UV-vis), onde valor encontrado foi de 32ºC.The present study aims to develop thermosensitive membranes with an intelligent mechanism of adhesion/release and potent antimicrobial action for the treatment of wounds. The membranes were prepared by electrosynthesis of the thermosensitive hydrogel poly (N-isopropylacrylamide) (PNIPAm) in the presence of chitosan (CHI). The material obtained is constituted by a semi-interpenetrating polymer network (sIPN) of CHI and PNIPAm. The chitosan is a natural biopolymer with activity bactericidal, anti-inflammatory and healing action. The commercial chitosan used was previously characterized in terms of its average molar mass (0.9312 * 105 g mol-1) by viscosimetric method and degree of deacetylation (86.23%), through conductometric titration. The PNIPAm hydrogel was incorporated to CHI polymer chain by electrochemical method using cyclic voltammetry technique. The sIPN CHI-PNIPAm membrane obtained was characterized by Fourier Transform Infrared Spectroscopy using the Attenuated Total Reflectance (FTIR-ATR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). FTIR-ATR spectra confirmed the polymerization of PNIPAm in the presence of CHI. TGA curve showed that sIPN membrane obtained has a composition of 33% chitosan and 55% PNIPAm. DSC thermal analysis showed a lower Tg of sIPN CHI-PNIPAm membrane compared to Tg of PNIPAm hydrogel. The phase transition temperature (LCST) of the sIPN CHI-PNIPAm membrane was determined by Ultaviolet-visible spectroscopy (UV-vis) the value found was 32 ° C.El presente trabajo busca desarrollar membranas termosensibles con un mecanismo inteligente de adhesión / liberación y potente acción antimicrobiana para el tratamiento de las heridas. Las membranas fueron preparadas a través de la electrossíntesis del hidrogel termossensible poli (N-isopropilacrilamida) (PNIPAm) en presencia de la quitosana (QUI). El material final constituye una red polimérica semi-interpenetrante (sIPN) de QUI y PNIPAm. La quitosana es un biopolímero natural que tiene acción bactericida, anti-inflamatoria y cicatrizante. La quitosana comercial utilizada fue previamente caracterizada en términos de su masa molar media (0,9312 * 105 g mol-1) por método viscosimétrico y grado de desacetilación (86,23%), a través de titulación conductimétrica. El hidrogel PNIPAm fue incorporado a la cadena polimérica de la QUI por vía electroquímica a través de la técnica de vueltametría cíclica. La membrana sIPN QUI-PNIPAm obtenida fue caracterizada por Espectroscopia en el infrarrojo por transformada de Fourier usando el modulo de Reflección Total Atenuada (FTIR-ATR), calorimetría exploratoria diferencial (DSC) y termogravimétrica (TG). El espectro FTIR-ATR confirmó la polimerización del PNIPAm en presencia de la QUI. La curva TG mostró que la membrana sIPN obtenida presenta una composición de 33% de quitosana y 55% de PNIPAm. El análisis térmico por DSC mostró que la Tg de la membrana sIPN QUI-PNIPAm es más baja que la Tg del hidrogel PNIPAm. La temperatura de transición de fase (LCST) de la membrana sIPN QUI-PNIPAm fue determinada por espectroscopia en la región del ultravioleta visible (UV-vis), donde el valor encontrado fue de 32ºC

Indium tin oxide synthesized by a low cost route as SEGFET pH sensor

Polycrystalline ITO films with good optoelectronics characteristics and homogeneous surface has been obtained upon annealing at 550 °C in N2 atmosphere using a low-cost chemical vapor deposition (CVD) system. The films were evaluated as pH sensors in separative extended gate field-effect transistor (SEGFET) apparatus, exhibiting a sensitivity of 53 mV/pH, close to the expected Nernstian theoretical value for ion sensitive materials. The use of CVD process to synthesize ITO, as described here, may represent an alternative for fabrication of SEGFET pH sensors at low cost to be used in disposable biosensors since H+ ions are the product of several oxireductase enzymes.CNPqCAPESFAPEMIGFAPES

Mechanisms of Resistance to Noncovalent Bruton's Tyrosine Kinase Inhibitors

BackgroundCovalent (irreversible) Bruton's tyrosine kinase (BTK) inhibitors have transformed the treatment of multiple B-cell cancers, especially chronic lymphocytic leukemia (CLL). However, resistance can arise through multiple mechanisms, including acquired mutations in BTK at residue C481, the binding site of covalent BTK inhibitors. Noncovalent (reversible) BTK inhibitors overcome this mechanism and other sources of resistance, but the mechanisms of resistance to these therapies are currently not well understood.MethodsWe performed genomic analyses of pretreatment specimens as well as specimens obtained at the time of disease progression from patients with CLL who had been treated with the noncovalent BTK inhibitor pirtobrutinib. Structural modeling, BTK-binding assays, and cell-based assays were conducted to study mutations that confer resistance to noncovalent BTK inhibitors.ResultsAmong 55 treated patients, we identified 9 patients with relapsed or refractory CLL and acquired mechanisms of genetic resistance to pirtobrutinib. We found mutations (V416L, A428D, M437R, T474I, and L528W) that were clustered in the kinase domain of BTK and that conferred resistance to both noncovalent BTK inhibitors and certain covalent BTK inhibitors. Mutations in BTK or phospholipase C gamma 2 (PLCγ2), a signaling molecule and downstream substrate of BTK, were found in all 9 patients. Transcriptional activation reflecting B-cell-receptor signaling persisted despite continued therapy with noncovalent BTK inhibitors.ConclusionsResistance to noncovalent BTK inhibitors arose through on-target BTK mutations and downstream PLCγ2 mutations that allowed escape from BTK inhibition. A proportion of these mutations also conferred resistance across clinically approved covalent BTK inhibitors. These data suggested new mechanisms of genomic escape from established covalent and novel noncovalent BTK inhibitors. (Funded by the American Society of Hematology and others.)

Redes epóxi/amina alifáticas com perspectivas para aplicações cardiovasculares. Propriedades biológicas in vitro

Este trabalho descreve as propriedades biológicas in vitro de três redes epoxídicas à base do éter diglicidílico do glicerol (DGEG) curadas com poli(oxipropileno) diamina (D230), isoforona diamina (IPD) e 4,4'-diamino-3,3'-dimetil-diciclohexilmetano (3DCM). As interações biológicas entre os polímeros e o sangue foram estudadas por ensaios biológicos in vitro. Estudos de adsorção de proteínas, adesão de plaquetas, atividade do lactato desidrogenase (LDH) e propriedades de tromboresistência estão apresentados. Os ensaios de adsorção de proteínas na superfície dos polímeros mostrou que as redes epoxídicas adsorvem mais albumina do que fibrinogênio. Os resultados relacionados à adesão de plaquetas, atividade do lactato hidrogenase e propriedades de tromboresistência indicaram que as redes DGEG/IPD e DGEG/3DCM exibem comportamento hemocompatível. Desta maneira, assumimos que estes polímeros epoxídicos são materiais compatíveis com o sangue

Updated cardiovascular prevention guideline of the Brazilian Society of Cardiology: 2019

Sem informação113478788