Mujeres y economía

En el marco de la globalización, cada vez en mayor medida las mujeres se hacen presentes en todos los ámbitos de la vida económica, política y social del país, desafiando a las instituciones que no consideran los problemas de igualdad de género y las aspiraciones de participación de la mujer. Al iniciarse el siglo XXI, las mujeres no tienen un sentimiento de triunfo, sino de inseguridad e incertidumbre, de insatisfacción y desconfianza ante su futuro. De ahí que la disminución de la inequidad, la erradicación de la pobreza y el establecimiento pleno de la justicia, del derecho a la igualdad y a la democracia, sean los componentes fundamentales de la transformación que debe experimentar la humanidad en el siglo que comienza. En este trabajo, en cuanto a medidas concretas, quizá lo más relevante sea la propuesta colectiva de aplicar un Programa de Pleno Empleo dirigido principalmente a la población femenina mayor de 15 años y menos instruida

Validation of a Quick Flow Cytometry-Based Assay for Acute Infection Based on CD64 and CD169 Expression. New Tools for Early Diagnosis in COVID-19 Pandemic

Objectives: Several parameters aid in deciphering between viral and bacterial infections; however, new tools should be investigated in order to reduce the time to results and proceed with an early target-therapy. Validation of a biomarker study, including CD64 and CD169 expression, was conducted. Material and Methods: Patients with active SARS-CoV-2 infection (ACov-2), bacterial infection (ABI), healthy controls, and antiretroviral-controlled chronic HIV infection were assessed. Whole blood was stained and, after lysing no-wash protocol, acquired by flow cytometry. The median fluorescence intensity (MFI) of CD64 and CD169 was measured in granulocytes, monocytes, and lymphocytes. The CD64 MFI ratio granulocytes to lymphocytes (CD64N) and CD169 MFI ratio monocytes to lymphocytes (CD169Mo) were evaluated as biomarkers of acute bacterial and viral infection, respectively. Results: A CD64N ratio higher than 3.3 identified patients with ABI with 83.3 and 85.9% sensitivity and specificity, with an area under the curve (AUC) of 83.5%. In contrast, other analytic or hematological parameters used in the clinic had lower AUC compared with the CD64N ratio. Moreover, a CD169Mo ratio higher than 3.3 was able to identify ACov-2 with 91.7 and 89.8 sensitivity and specificity, with the highest AUC (92.0%). Conclusion: This work confirms the previous data of CD64N and CD169Mo ratios in an independent cohort, including controlled chronic viral HIV infection patients as biomarkers of acute bacterial and viral infections, respectively. Such an approach would benefit from quick pathogen identification for a direct-therapy with a clear application in different Health Care Units, especially during this COVID pandemic.This work was partially supported by Instituto de Salud Carlos III Grant: FIS (COV20/0170

Non-criteria obstetric antiphospholipid syndrome: how different is from Sidney criteria? A single-center study

This study aims to compare the demographic characteristics, clinical features, serology, and fetal-maternal outcomes between women with obstetric antiphospholipid syndrome (APS) and those with non-criteria (NC)-APS and seronegative (SN)-APS. Two-hundred and sixty-three women with APS obstetric morbidity ever pregnant were included. Of those, 66 met the APS classification criteria, 140 were NC-APS, and 57 were SN-APS. Patients with other autoimmune diseases were excluded. Adverse pregnancy outcomes (APO) included early pregnancy loss, fetal death, preeclampsia, abruptio placentae, and preterm birth. The mean age of the study group was 33.6 ± 5.3 years, and patients were followed up for 129.5 ± 81.9 months. In the NC-APS group, 31 (22.1%) did not fulfill clinical and serological criteria (Subgroup A), 49 (35%) did meet clinical but not serologic criteria (Subgroup B), and 60 (42.9%) fulfilled the serologic criteria but not the clinical ones (Subgroup C). The cardiovascular risk burden was higher in the APS group, due to a higher proportion of smoking. Patients with criteria APS received more intensive treatment than patients in the other study groups. The addition of standard of care (SoC) treatment significantly improved live birth and decreased APO in all groups. Significant clinical differences were observed between the study groups. However, when treated with SoC, fetal-maternal outcomes were similar, with a significant improvement in live births and a decrease in APO. Risk stratification in patients with obstetric morbidity associated with APS can help individualize their treatment

Immune Assessment of BNT162b2 m-RNA-Spike Based Vaccine Response in Adults

Vaccine efficacy is based on clinical data. Currently, the assessment of immune response after SARS-CoV-2 vaccination is scarce. A total of 52 healthcare workers were immunized with the same lot of BNT162b2 vaccine. The immunological response against the vaccine was tested using a T-specific assay based on the expression of CD25 and CD134 after stimulation with anti-N, -S, and -M specific peptides of SARS-CoV-2. Moreover, IgG anti-S2 and -RBD antibodies were detected using ELISA. Furthermore, the cell subsets involved in the response to the vaccine were measured in peripheral blood by flow cytometry. Humoral-specific responses against the vaccine were detected in 94% and 100% after the first and second doses, respectively. Therefore, anti-S T-specific responses were observed in 57% and 90% of the subjects after the first and second doses of the vaccine, respectively. Thirty days after the second dose, significant increases in T helper 1 memory cells (p < 0.001), peripheral memory T follicular helper (pTFH) cells (p < 0.032), and switched memory (p = 0.005) were observed. This study describes the specific humoral and cellular immune responses after vaccination with the new mRNA-based BNT162b2 vaccine. A mobilization of TFH into the circulation occurs, reflecting a specific activation of the immune system.Funding: This work was partially supported by the Cantabrian Government, grant number 2020UIC22-PUB-001, and from Instituto de Salud Carlos III, grant number COV20/00170

COVID-19 mRNA Based Vaccine Immune-Response Assessment in Nursing Home Residents for Public Health Decision

Nursing home residents (NHR) have been targeted as a vaccination priority due to their higher risk of worse outcome after COVID-19 infection. The mRNA-based vaccine BTN2b2 was first approved in Europe for NHRs. The assessment of the specific vaccine immune response (both humoral and cellular) at long term in NHRs has not been addressed yet. A representative sample of 624 NHR subjects in Northern region of Spain was studied to assess immune response against full vaccination with BTN2b2. The anti-S1 antibody levels and specific T cells were measured at two and six months after vaccination. 24.4% of NHR had a previous infection prior to vaccination. The remaining NHR were included in the full vaccination assessment group (FVA). After two months, a 94.9% of the FVA presented anti-S1 antibodies, whereas those seronegative without specific cellular response were 2.54%. At long-term, the frequency of NHR within the FVA group with anti-S1 antibodies at six months were 88.12% and the seronegative subjects without specific cellular response was 8.07%. The cellular immune assays complement the humoral test in the immune vaccine response assessment. Therefore, the cellular immune assessment in NHRs allows for the fine tuning of those seronegative subjects with potential competent immune responses against the vaccine

Innate and adaptive immune assessment at admission to predict clinical outcome in covid-19 patients

During the COVID-19 pandemic, many studies have been carried out to evaluate different immune system components to search for prognostic biomarkers of the disease. A broad multiparametric antibody panel of cellular and humoral components of the innate and the adaptative immune response in patients with active SARS-CoV-2 infection has been evaluated in this study. A total of 155 patients were studied at admission into our center and were categorized according to the requirement of oxygen therapy as mild or severe (the latter being those with the requirement). The patients with severe disease were older and had high ferritin, D-dimer, C-reactive protein, troponin, interleukin-6 (IL-6) levels, and neutrophilia with lymphopenia at admission. Moreover, the patients with mild symptoms had significantly increased circulating non-classical monocytes, innate lymphoid cells, and regulatory NK cells. In contrast, severe patients had a low frequency of Th1 and regulatory T cells with increased activated and exhausted CD8 phenotype (CD8+CD38+HLADR+ and CD8+CD27-CD28-, respectively). The predictive model included age, ferritin, D-dimer, lymph counts, C4, CD8+CD27-CD28-, and non-classical monocytes in the logistic regression analysis. The model predicted severity with an area under the curve of 78%. Both innate and adaptive immune parameters could be considered potential predictive biomarkers of the prognosis of COVID-19 disease.Funding: This work was partially supported by the Cantabrian Government, grant number 2020UIC22-PUB-001, and by Instituto de Salud Carlos III, grant number COV20/00170

PROF-XXI: Teaching and Learning Centers to Support the 21st Century Professor

PROF-XXI is a European-funded project whose aim is the creation of Teaching and Learning Centers (TLCs) for Latin American Higher Institutions in an effort to promote the development of competences for university professors and foster teaching innovation in onsite, but also in online and hybrid education. PROF-XXI includes a partnership of seven higher education institutions, three from European countries (Spain, France, and Portugal), and four from Latin American countries (two from Guatemala, and two from Colombia). This article presents the main results of the first part of the project, including the diagnosis of institutional practices, the state of the art of TLCs around the world, the framework on 21st century professors in Latin America, and the PROF-XXI framework

Current treatment of type 2 diabetes mellitus

La Diabetes Mellitus tipo 2 es una enfermedad crónica degenerativa e incurable pero controlable más frecuente en todo el mundo y un problema de salud pública debido a sus complicaciones crónicas y agudas. Existen varios objetivos en el tratamiento del diabético tipo 2, pero su esencia radica en el control metabólico y la prevención de las complicaciones. La revisión abordó la necesidad de modificar estilos de vida, sintetiza algunas características farmacológicas en cada grupo, se mostró un novedoso arsenal terapéutico como alternativa de impacto. Propuso la combinación de dos y tres medicamentos hipoglucemiantes con la finalidad de alcanzar niveles deseados de glucemias y disminuir reacciones adversas. Expuso un algoritmo integrador para el manejo práctico de la diabetes, que favorece el desempeño médico en la atención primaria y secundaria. Se concluye que existen medidas preventivas que facilitan la disminución de la prevalencia y morbimortalidad de las personas con diabetes mellitus tipo 2.Type 2 Diabetes Mellitus is the most frequent chronic degenerative and incurable but controllable disease worldwide and a public health problem due to its chronic and acute complications. There are several objectives in the treatment of type 2 diabetics, but its essence lies in metabolic control and the prevention of complications. The review addressed the need to modify lifestyles, synthesizes some pharmacological characteristics in each group, and showed a novel therapeutic arsenal as an impact alternative. He proposed the combination of two and three hypoglycemic drugs in order to achieve desired blood glucose levels and reduce adverse reactions. He presented an integrative algorithm for the practical management of diabetes, which favors medical performance in primary and secondary care. It is concluded that there are preventive measures that facilitate the decrease in the prevalence and morbidity and mortality of people with type 2 diabetes mellitus

Cátedra abierta de la interculturalidad en territorios de paz : capítulo 1: semillas, alimentos y comida. Una lectura intercultural

CD-T 306.4 S55; 146 p CD-T 306.4 S55c; 142 pEl proyecto catedra abierta de la interculturalidad de la Universidad Libre construye escenarios de paz para la construcción de nuestra identidad nacional en tres municipios : La Virginia, Mistrató, Pereira; hoy se logra llegar a estos tres municipios y con estos se inicia el camino de la mano de niñas y niños de centros educativas urbanos y rurales; el sector educativo caracterizó el inicio histórico y el tema pre-texto fue: las semillas, los alimentos y la comida, tema qué esta propuesto desde el primer encuentro intercultural realizado en el municipio de Mistrató, con la participación activa de comunidades nativas y del fundador de la catedra el TAITA JAVIER CALAMBAS, de la comunidad indígena MISAK del Cauca. El proceso de la catedra se orientó con la metodología MARDIC, la cual propicio la participación activa de grupos organizados denominados grupos focales.Universidad Libre Seccional Pereir