3 research outputs found
Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function
- Author
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- Zeef Leo
- Zoma Asad
- Publication venue
- Publication date
- 02/08/2017
- Field of study
Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes
Synthesis and styrene copolymerization of novel alkoxy ring-substituted isobutyl phenylcyanoacrylates
- Author
- Publication venue
- Publication date
- 21/02/2022
- Field of study
Novel alkoxy ring-substituted isobutyl phenylcyanoacrylates, RPhCH=C(CN)CO2CH2CH(CH3)2 (where R is 2-methoxy, 3-methoxy, 4-methoxy, 2-ethoxy, 3-ethoxy, 4-ethoxy, 4-propoxy, 4-butoxy, 4-hexyloxy) were prepared and copolymerized with styrene. The acrylates were synthesized by the piperidine catalyzed Knoevenagel condensation of ring-substituted benzaldehydes and isobutyl cyanoacetate and characterized by CHN elemental analysis, IR, 1H- and 13C-NMR. All the acrylates were copolymerized with styrene in solution with radical initiation (ABCN) at 70C. The composition of the copolymers was calculated from nitrogen analysis, and the structures were analyzed by IR, 1H and 13C-NMR. Thermal properties of the copolymers are characterized by DSC and TGA. Decomposition of the copolymers in nitrogen occurred in two steps, first in the 200-500ºC range with residue (1.8-3.3% wt.), which then decomposed in the 500-800ºC range
1994 Annual Selected Bibliography: Asian American Studies and the Crisis of Practice
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- Jennings James
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- Jha Prabhat
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- Johnson Graham E
- Joshi Aida A
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- Ka
- Kagawa-Singer Marjorie
- Kahn Joan R
- Kame'eleihiwa Lilikala
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- Kleiber Margrethe S. C.
- Klootwijk Eeuwout
- Knox Robert J
- Kodama Masaaki
- Koehn Sharon Denise
- Koh Howard Kyongju
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- Korean YellowPages
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- Koshy Susan
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- Krieger Nancy
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- Kulig Judith C
- Kulig Judith C
- Kunin Roslyn
- Kwa Lydia
- Kwong Peter
- Laczko Leslie S
- Lai Walton Look
- Lam Andrew
- Lam K.-C.
- Lam Leo L
- Lam Maivbn Clech
- Lambert Richard D
- Lamphere Louise
- Landau Elaine
- Lange Cynthia Juarez
- Lary Diana
- Lau Evelyn
- Lau Evelyn
- Lau Wendy
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- Le Ahn
- Le Tri Huu
- Lee
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- Lee Clifford
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- Lee Elisa
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- Lee Gus
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- Leong Frederick T. L.
- Leong Frederick T. L.
- Leong Russell C
- Leong Russell C
- Lese Karen P
- Leung S. Alvin
- Levine D. M.
- Levy Daniel
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- Li
- Li Guangming
- Li X. L.
- Liang Zai
- Light I.
- Light I.
- Lim Gerard
- Lim Shirley
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- Lin Josh C. H.
- Lin-Fu J. S.
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- Lind E. Allan
- Linehan T. M.
- Ling Huping
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- Lippi-Green Rosina
- Lipske Mike
- Lipton S. Gregory.
- Little Gom-Benn
- Liu Aimee E
- Livingston Gary R
- Lo S. D.
- Lopez I. F.
- Los Angeles County
- Lott Juanita Tamayo
- Louie
- Low Jeanie W. C.
- Lowe Sharon
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- Lucas Tamara
- Ludman E. K. K. J.
- Lurie Rod
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- Madden Ryan
- Mahtani Minelle K
- Mak Anita S
- Malicky Grace V
- Maltz Earl M
- Manalansan Martin F. IV.
- Mandell Phyllis Levy
- Mangaoang Gil
- Manley Theodoric Jr
- Marable Manning
- Marie Calude-Valentin
- Marignay Bisola
- Martin
- Martinez Ramiro
- Mason Jim
- Massey Douglas S
- Masson Mireille Andree
- Matejko Alexander J
- Mathis Darlene
- Matloff Norman
- Matsukawa Yuko
- Matsumoto Valerie J
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- McKenney Nampeo R
- McLellan Janet
- McMahon Anthony
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- Medina T. G.
- Meiklejohn Civil Liberties Institute
- Melvani Lavina
- Melwani Lavina
- Melwani Lavina
- Mendez Carlos
- Menton
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- Messaris P.
- Metoyer-Duran Cheryl
- Meyer Richard E
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- Mori Teruyo
- Morikawa Jitsuo
- Morris
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- Morrow H. W.
- Mortland Carol A
- Moseley Romney
- Moss David M
- Mukherjee Arun
- Multicultural History Society on Ontario 12
- Munger Ronald G
- Murayama Milton
- Murphy William J
- Muse Daphne
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- Nagata Donna K
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- Nah Kyung-Hee
- Nair Savitry
- Nakanishi Don T
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- National Asian American Telecommunications Association.
- National Asian Pacific American Legal Consortium.
- Naturalization Service
- Nautu Dorri
- Nayyar Deepak
- Nee Victor
- Nelson Emmanuel
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- Pau
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- Pellerin Helene
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- Planting Seeds Coalition
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- San Juan E. Jr.
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- San Juan E. Jr.
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- Schoenberger Karl
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- Seattle
- Seattle Chinese Yellow Pages
- Selected Bibliography
- Sensitivity
- Serran Tony
- Serxner Seth
- Sev'er Aysan
- Sexton
- Sexton Ken
- Sexton Ken
- Shaffer Donald R
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- Sovereignty
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- St. Raphael's Parish
- Stanfield J. H.
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- Stoos
- Straubhaar T.
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- Strona Proserfina A
- Strong James
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- Taylor Lawrence Douglas H
- TDR
- Tehranian Katharine
- Tehranian Majid
- The Filipino Directory
- The Peoples Multicultural Almanac
- the United States
- The Western Massachusetts Vietnamese Association Directory (Springfield Massachusetts
- Thobani Sunera
- Tien Liang
- Tirrito Terry
- Tobin Jeffrey
- Tohmatsu Akiko Judith
- Tong Benson
- Totten George O
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- Tran Qui-Phiet
- Tran Thanh V
- Trask Haunani-Kay
- Trask Mililani
- Trevison Catherine
- Truong Monique
- Tsang Daniel C
- Tseng Yen-Fen
- Tsuchida John Nobuya
- Turner Marlene
- Tuttle C. R.
- Tworkov Helen
- U.S. Department of State
- Uba Laura
- Ueda Reed
- Uehara Edwina S
- Ueki Teruyo
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- Vander Kathryn
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- Vera Arleen
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- Vietnamese Consumers
- Villalba
- Volpp Leti
- Wake Barbara
- Waldinger Roger
- Waldinger Roger
- Waldinger Roger
- Wallace Patricia
- Walter Pierre
- Walz Eric
- Wang
- Wang Gungwu
- Wang Linna
- Wang Ping
- Wang Quingjia Edward
- Wang V.
- Wang Y .
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- Wetherell
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- Wial Howard
- Wiecha John M
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- Wilkinson Alec
- Willard Walter
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- Winn Nancy Nishimura
- Wolf Eric R
- Wong Cynthia
- Wong Deborah
- Wong Gil
- Wong L. Mun.
- Wong William
- Wong-Liane Eirene Ming
- Woo Alan H
- Woods Nancy Fugate
- Workplace
- Wright Debbie Hippolite
- Wright Robert E
- Wu Edna
- Wu Jenai
- Wu T. C.
- Xiong Phua
- Ya Va Thai
- Yamamoto Hisaye
- Yamamoto Joe
- Yamamoto Yasushi
- Yamanaka Keiko
- Yamanaka Lois-Ann
- Yamashita Kanshi Stanley
- Yamato Alexander
- Yamauchi Wakako
- Yan Wenfan
- Yang Dao
- Yang Jeff
- Yang P.
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- Yang Philip Q
- Yang Xiaoyun
- Yasmin Marziya
- Yau Jenny
- Ye Weili
- Yee A. H.
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- Yeh May
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- Yi Jenny Kisuk
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- Yong Fung L
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- Yoshida Ryo
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- Yu Diana
- Yu Eui-Young
- Yu M. C.
- Zackrison James Willard
- Zaharlick Amy
- Zane Nolan
- Zane Nolan
- Zane Nolan
- Zhou Min
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- Zlotkin Hania
- Zuvekas Ann
- Publication venue
- 'UCLA American Indian Studies Center'
- Publication date
- Field of study