623 research outputs found
Human cerebral organoids and neural 3D tissues in basic research, and their application to study neurological diseases
descripciĂłn no proporcionada por scopusSpanish Ministry of Science, Economy and Competitiveness, call Retos, Ref: SAF2017-83241-R
Functionalization and Characterization of Magnetic Nanoparticles for the Detection of Ferritin Accumulation in Alzheimer's Disease
Early diagnosis in Alzheimer's disease (AD), prior to the appearance of marked clinical symptoms, is critical to prevent irreversible neuronal damage and neural malfunction that lead to dementia and death. Therefore, there is an urgent need to generate new contrast agents which reveal by a noninvasive method the presence of some of the pathological signs of AD. In the present study, we demonstrate for the first time a new nanoconjugate composed of magnetic nanoparticles bound to an antiferritin antibody, which has been developed based on the existence of iron deposits and high levels of the ferritin protein present in areas with a high accumulation of amyloid plaques (particularly the subiculum in the hippocampal area) in the brain of a transgenic mouse model with five familial AD mutations. Both in vitro and after intravenous injection, functionalized magnetic nanoparticles were able to recognize and bind specifically to the ferritin protein accumulated in the subiculum area of the AD transgenic mice.Fil: Fernández Cabada, Tamara. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad PolitĂ©cnica de Madrid; EspañaFil: MartĂnez Serrano, Alberto. Consejo Superior de Investigaciones CientĂficas; España. Universidad AutĂłnoma de Madrid; EspañaFil: CussĂł, Lorena. Instituto de Investigacion Sanitaria Gregorio Marañón; España. Universidad Carlos III de Madrid; España. Centro de InvestigaciĂłn BiomĂ©dica en Red de Salud Mental; EspañaFil: Desco, Manuel. Instituto de Investigacion Sanitaria Gregorio Marañón; España. Centro de InvestigaciĂłn BiomĂ©dica en Red de Salud Mental; España. Universidad Carlos III de Madrid; EspañaFil: Ramos GĂłmez, Milagros. Universidad PolitĂ©cnica de Madrid; Españ
Multifactoriality of Parkinson’s disease as explored through human neural stem cells and their transplantation in middle-aged parkinsonian mice
Parkinson’s disease (PD) is an age-associated neurodegenerative disorder for which there is currently no cure. Cell replacement therapy is a potential treatment for PD; however, this therapy has more clinically beneficial outcomes in younger patients with less advanced PD. In this study, hVM1 clone 32 cells, a line of human neural stem cells, were characterized and subsequently transplanted in middle-aged Parkinsonian mice in order to examine cell replacement therapy as a treatment for PD. In vitro analyses revealed that these cells express standard dopamine-centered markers as well as others associated with mitochondrial and peroxisome function, as well as glucose and lipid metabolism. Four months after the transplantation of the hVM1 clone 32 cells, striatal expression of tyrosine hydroxylase was minimally reduced in all Parkinsonian mice but that of dopamine transporter was decreased to a greater extent in buffer compared to cell-treated mice. Behavioral tests showed marked differences between experimental groups, and cell transplant improved hyperactivity and gait alterations, while in the striatum, astroglial populations were increased in all groups due to age and a higher amount of microglia were found in Parkinsonian mice. In the motor cortex, nonphosphorylated neurofilament heavy was increased in all Parkinsonian mice. Overall, these findings demonstrate that hVM1 clone 32 cell transplant prevented motor and non-motor impairments and that PD is a complex disorder with many influencing factors, thus reinforcing the idea of novel targets for PD treatment that tend to be focused on dopamine and nigrostriatal damag
Group i metabotropic glutamate receptors: A potential target for regulation of proliferation and differentiation of an immortalized human neural stem cell line
© 2014 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society). Human neural stem cells (NSCs) from the developing embryo or the subventricular zone of the adult brain can potentially elicit brain repair after injury or disease, either via endogenous cell proliferation or by cell transplantation. Profound knowledge of the diverse signals affecting these cells is, however, needed to realize their therapeutic potential. Glutamate and group I metabotropic glutamate receptors (mGluRs) affect proliferation and survival of rodent NSCs both during embryonic and post-natal development. To investigate the role of group I mGluRs (mGluR1 and mGluR5) on human NSCs, we differentiated an immortalized, forebrain-derived stem cell line in the presence or absence of glutamate and with addition of either the group I mGluR agonist DHPG or the selective antagonists, MPEP (mGluR5) and LY367385 (mGluR1). Characterization of differentiated cells revealed that both mGluR1 and mGluR5 were present on the cells. Addition of glutamate to the growth medium significantly increased cell proliferation and reduced cell death, resulting in increased cell numbers. In the presence of glutamate, selective activation of group I mGluRs reduced gliogenesis, whereas selective inhibition of group I mGluRs reduced neurogenesis. Our results substantiate the importance of glutamate signalling in the regulation of human NSCs and may as such be applied to promote proliferation and neuronal differentiation.This research was supported by the Danish Parkinson Association, IMK Almene Fond, Hørslev-Fonden, Kirsten og Freddy Johansens Fond, Grosserer Brogaard og Hustrus Mindefond and Fonden for Lægevidenskabens Fremme.Peer Reviewe
MIRACLE evaluation of results for ImageCLEF 2003
ImageCLEF is a new pilot experiment introduced in CLEF 2003. It is devoted to the cross language retrieval of images using textual descriptions related to images contents. This paper
presents MIRACLE research team experiments and results obtained for this track
V-Myc immortalizes human neural stem cells in the absence of pluripotency-associated traits
© 2015 Pino-Barrio et al. A better understanding of the molecular mechanisms governing stem cell self-renewal will foster the use of different types of stem cells in disease modeling and cell therapy strategies. Immortalization, understood as the capacity for indefinite expansion, is needed for the generation of any cell line. In the case of v-myc immortalized multipotent human Neural Stem Cells (hNSCs), we hypothesized that v-myc immortalization could induce a more dedifferentiated state in v-myc hNSC lines. To test this, we investigated the expression of surface, biochemical and genetic markers of stemness and pluripotency in v-myc immortalized and control hNSCs (primary precursors, that is, neurospheres) and compared these two cell types to human Embryonic Stem Cells (hESCs) and fibroblasts. Using a Hierarchical Clustering method and a Principal Component Analysis (PCA), the v-myc hNSCs associated with their counterparts hNSCs (in the absence of v-myc) and displayed a differential expression pattern when compared to hESCs. Moreover, the expression analysis of pluripotency markers suggested no evidence supporting a reprogramming-like process despite the increment in telomerase expression. In conclusion, v-myc expression in hNSC lines ensures self-renewal through the activation of some genes involved in the maintenance of stem cell properties in multipotent cells but does not alter the expression of key pluripotency-associated genes.Spanish Ministry of Economy and Competitiveness (PLE2009–0101, SAF2010–17167); Comunidad Autónoma Madrid (S2011—BMD—2336); Instituto Salud Carlos III (RETICS TerCel, RD12/0019/0013) and European Union (Excell, NMP4—SL—2008–214706); (to PM): Instituto Salud Carlos III (RETICS TerCel, RD12/0019/0006; FISPeer Reviewe
Evaluation of MIRACLE approach results for CLEF 2003
This paper describes MIRACLE (Multilingual Information RetrievAl for the CLEf campaign) approach and results for the mono, bi and multilingual Cross Language Evaluation Forum tasks. The approach is based on the combination of linguistic and statistic techniques to perform indexing and retrieval tasks
V-Myc immortalizes human neural stem cells in the absence of pluripotency-associated traits
The data discussed in
this publication have been deposited in NCBI’s Gene
Expression Omnibus (GEO) and are accessible
through GEO Series accession number GSE63710A better understanding of the molecular mechanisms governing stem cell self-renewal
will foster the use of different types of stem cells in disease modeling and cell therapy strategies.
Immortalization, understood as the capacity for indefinite expansion, is needed for the
generation of any cell line. In the case of v-myc immortalized multipotent human Neural
Stem Cells (hNSCs), we hypothesized that v-myc immortalization could induce a more dedifferentiated
state in v-myc hNSC lines. To test this, we investigated the expression of surface,
biochemical and genetic markers of stemness and pluripotency in v-myc immortalized
and control hNSCs (primary precursors, that is, neurospheres) and compared these two
cell types to human Embryonic Stem Cells (hESCs) and fibroblasts. Using a Hierarchical
Clustering method and a Principal Component Analysis (PCA), the v-myc hNSCs associated
with their counterparts hNSCs (in the absence of v-myc) and displayed a differential expression
pattern when compared to hESCs. Moreover, the expression analysis of
pluripotency markers suggested no evidence supporting a reprogramming-like process despite
the increment in telomerase expression. In conclusion, v-myc expression in hNSC
lines ensures self-renewal through the activation of some genes involved in the maintenance
of stem cell properties in multipotent cells but does not alter the expression of key
pluripotency-associated genesThis work was supported by grants from (to
AMS): Spanish Ministry of Economy and
Competitiveness (PLE2009–0101, SAF2010–17167),
Comunidad Autónoma Madrid (S2011—BMD—
2336), Instituto Salud Carlos III (RETICS TerCel,
RD12/0019/0013) and European Union (Excell,
NMP4—SL—2008–214706); (to PM): Instituto Salud
Carlos III (RETICS TerCel, RD12/0019/0006; FIS
P110/0449), ERANEt ISCIII—Fondos FEDER (PI12/
03112) and the Spanish Association of Cancer
Research (CIMEN2011). MJPB was funded by
MINECO (PLE2009–0101) and Instituto Salud Carlos
III (RETICS TerCel, RD06/0019/0023). This work was
also supported by an institutional grant from
FundaciĂłn RamĂłn Areces to the Center of Molecular
Biology Severo Ochoa. PM lab is supported by:
Instituto de Salud Carlos III (ISCIII; E-Rare-2 Call
PI12/03112), Ministerio de EconomĂa y
Competitividad (MINECO; SAF2013-43065),
Generalitat de Catalunya (SGR330) and Obra Social
La Caixa-Fundaciò Josep Carrera
Integrating cognitive contents in Physical Education classes: Effects on cognitive variables and emotional intelligence
The aim was to analyse the effect of a 4-week programme integrating cognitive contents in Physical Education (CogniPE) on cognitive performance (CP) and emotional intelligence (EI) of adolescents. A randomised controlled trial was conducted with a control group (CG, n=58), which performed physical exercises at low intensity (i.e., stretching or pilates), an experimental group 1, which performed small-sided games of team sports (EG1, n=62), and experimental group 2, which performed CogniPE (EG2, n=60). Intensity of exercises and scores were registered after each station to classify and motivate the teams. Selective attention and concentration increased by 11.9% and 9.2% in EG1, and by 18.2% and 14.4% in EG2, respectively, compared to CG. Mathematical calculation improved by 15.9% and 18.7% in EG1 and EG2, compared to CG. In EI, well-being improved by 10.9% in EG1, sociability improved by 12.8% in EG1 and 15.9% in EG2 compared to CG. It is concluded to use CogniPE in school context
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