1,844 research outputs found
Unusual flexibility of mesophase pitch-derived carbon materials:an approach to the synthesis of graphene
Structural flexibility in a petroleum pitch-derived carbon material has been indirectly evaluated using X-ray diffraction (XRD), immersion calorimetry and inelastic neutron scattering (INS) measurements. Exposure of the carbon material to an organic solvent (e.g., n-nonane) gives rise to a large internal rearrangement, associated with a drastic re-ordering of the graphitic microdomains. These structural changes are also associated with a high flexibility of the internal porous network, as observed by inelastic neutron scattering measurements. The internal rearrangement and the structural flexibility could be responsible for the excellent performance of this kind of activated carbons in a wide variety of adsorption processes. Last but not least, the structural characteristics of these carbon materials composed of graphitic microdomains has been used to synthesize graphene “egg-like” flakes following a simple procedure based on exfoliation with organic solvents
Hipotiroidismo subclínico y factores de riesgo cardiovascular
Objetivo: Conocer la prevalencia del hipotiroidismo
subclínico en la población general de un centro de salud
urbano y describir las características clínicas y factores
de riesgo cardiovascular de los pacientes con hipotiroidismo
subclínico.
Métodos: Se realizó un estudio observacional descriptivo,
transversal, retrospectivo, revisando las historias clínicas
de los pacientes incluidos en la muestra desde junio de 2005
hasta julio de 2007. Se analizaron las siguientes variables;
Datos generales: edad y sexo. Antecedentes familiares:
patología tiroidea y otras enfermedades. Antecedentes personales:
cardiovasculares, pulmonares, enfermedades
autoinmunes, alteraciones gineco-obstétricas, diabetes,
hipertensión (HT), dislipemia, obesidad, alteraciones psiquiátricas
y hematológicas. Datos de laboratorio: niveles
de TSH, niveles de T4 libre,presencia de anticuerpos antiperoxidasa,
niveles de colesterol total y sus fracciones.
Resultados: La prevalencia de la muestra de 100
pacientes recogida durante 8 meses fue de 3,8% de la
población general mayor de 14 años, de la cual 79 eran
mujeres y 21 eran hombres. El 13% eran diabéticos tipo
2, 23% tenían HT y un 40% tenían dislipemia. Sobrepeso
y obesidad estaban presentes en un 26%. El nivel medio
de TSH fue 6.92 ± 2,29 U/ml y el nivel medio de T4 libre
fue 1,16 ± 0,16 ng/ml.
Conclusiones: La prevalencia del hipotiroidismo subclínico
fue 3,8%. sobre todo en mujeres con una edad
media de 46 años. La incidencia de factores de riesgo cardiovascular
en los sujetos estudiados es mayor en DM
(13%), similar a la población general en cuanto a la dislipemia
(40%) y obesidad (20%) y menor en HTA (23%).
En nuestro estudio no se observa una pauta común en
el manejo del hipotiroidismo subclínico, siendo necesaria
la implementación y promoción de guías de actuación en
Atención PrimariaObjective: To determine the prevalence of subclinical
hypothyroidism in the general population of an urban
health center and describe the clinical characteristics and
cardiovascular risk factors in patients with subclinical
hypothyroidism.
Methods: An observational study, retrospective, reviewing
the medical histories of patients sampled from June
2005 until July 2007. We analyzed the following variables;
facts: age and sex. Family history thyroid disease
and other diseases. Personal history: cardiovascular pulmonary
autoimmune, alterations gynecology obstetric
diabetes, hypertension (HT) dislipemia, obesity, psychiatric
alterations and haematological. Laboratory data:
novel TSH, free T4, antiperoxidase antibodies, total cholesterol
and its fractions.
Results: The prevalence of the sample of 100 patients
collected over 8 months was 3.8% in the general population
over 14 years, of which 79 were women and 21 were
men. 13% were type 2 diabetics, 23% had HT and 40%
had dyslipidemia. Overweight and obesity were present
in 26%. The average level of TSH was 6.92 ± 2.29 U/ml
and the average level of free T4 was 1.16 ± 0.16 ng/ml.
Conclusions: Prevalence subclinical hypothyroidism
was 3.8%. especially in women with a mean age of 46. The
incidence of cardiovascular risk factors in the subjects
studied is higher in DM (13%), similar to general population
in terms of dyslipidemia (40%) and obesity (23%)
and lowest in hypertension (23%). In our study we observed
a common pattern in the management of subclinical
hypothyroidism, requiring the implementation and promotion
of practice guidelines in primary car
Estudio de la reacción entre el Hg(l) y el ácido etilenglicol bis (β-amino etil éter) tetraacético. Determinación espectrofotométrica de Hg22+
The reaction between Hg(I) and ethyleneglycol bis (β-aminoethylether)- N, N, N3, N3 tetraacetate (EGTA) in a weakly acidic médium (pH ≈ 5) is studied by U. V. spectrophotometric techniques. The maxiraum of absorption which appears at 247 nm is supposed due to the formation of a Hg(I) - EGTA complex. This compotmd is unstable and it decomposes slowly with formation of Hg(II) chelate and metallic mercury. When the absorbance is measured at fixed time (5 minutes), it is fovmd that Beer's law is obeyed from 1 up to 30 p p m of Hg(I) at 247 nm
Unusual flexibility of mesophase pitch-derived carbon materials:an approach to the synthesis of graphene
Structural flexibility in a petroleum pitch-derived carbon material has been indirectly evaluated using X-ray diffraction (XRD), immersion calorimetry and inelastic neutron scattering (INS) measurements. Exposure of the carbon material to an organic solvent (e.g., n-nonane) gives rise to a large internal rearrangement, associated with a drastic re-ordering of the graphitic microdomains. These structural changes are also associated with a high flexibility of the internal porous network, as observed by inelastic neutron scattering measurements. The internal rearrangement and the structural flexibility could be responsible for the excellent performance of this kind of activated carbons in a wide variety of adsorption processes. Last but not least, the structural characteristics of these carbon materials composed of graphitic microdomains has been used to synthesize graphene “egg-like” flakes following a simple procedure based on exfoliation with organic solvents
Estudio de la reacción entre el Cr(lll) y el ADCT catalizada por el CO3H1-. Determinación cinética de CO3H1-
A new kinetic method for the determination of CO3H1- is presented. It is based on the catalytic effect that this anión produces on the slow reaction of complexation between Cr(III) and DCyTA. The influence of different variables on the process, the effect of interferent ions and the mechanism of the reaction are studied. CO3H1- may be determined between 2-500 p.p.m. with good analytical results. The method has been appHed for the analysis of combined CO2 in natural waters
BCR-ABL induces the expression of Skp2 through the PI3K pathway to promote p27Kip1 degradation and proliferation of chronic myelogenous leukemia cells
Chronic myelogenous leukemia (CML) is characterized by the expression of the BCR-ABL tyrosine kinase, which results in increased cell proliferation and inhibition of apoptosis. In this study, we show in both BCR-ABL cells (Mo7e-p210 and BaF/3-p210) and primary CML CD34+ cells that STI571 inhibition of BCR-ABL tyrosine kinase activity results in a G(1) cell cycle arrest mediated by the PI3K pathway. This arrest is associated with a nuclear accumulation of p27(Kip1) and down-regulation of cyclins D and E. As a result, there is a reduction of the cyclin E/Cdk2 kinase activity and of the retinoblastoma protein phosphorylation. By quantitative reverse transcription-PCR we show that BCR-ABL/PI3K regulates the expression of p27(Kip1) at the level of transcription. We further show that BCR-ABL also regulates p27(Kip1) protein levels by increasing its degradation by the proteasome. This degradation depends on the ubiquitinylation of p27(Kip1) by Skp2-containing SFC complexes: silencing the expression of Skp2 with a small interfering RNA results in the accumulation of p27(Kip1). We also demonstrate that BCR-ABL cells show transcriptional up-regulation of Skp2. Finally, expression of a p27(Kip1) mutant unable of being recognized by Skp2 results in inhibition of proliferation of BCR-ABL cells, indicating that the degradation of p27(Kip1) contributes to the pathogenesis of CML. In conclusion, these results suggest that BCR-ABL regulates cell cycle in CML cells at least in part by inducing proteasome-mediated degradation of the cell cycle inhibitor p27(Kip1) and provide a rationale for the use of inhibitors of the proteasome in patients with BCR-ABL leukemias
Methodological approaches to determining the marine radiocarbon reservoir effect
The marine radiocarbon reservoir effect is an offset in 14C age between contemporaneous organisms from the terrestrial environment and organisms that derive their carbon from the marine environment. Quantification of this effect is of crucial importance for correct calibration of the <sup>14</sup>C ages of marine-influenced samples to the calendrical timescale. This is fundamental to the construction of archaeological and palaeoenvironmental chronologies when such samples are employed in <sup>14</sup>C analysis. Quantitative measurements of temporal variations in regional marine reservoir ages also have the potential to be used as a measure of process changes within Earth surface systems, due to their link with climatic and oceanic changes. The various approaches to quantification of the marine radiocarbon reservoir effect are assessed, focusing particularly on the North Atlantic Ocean. Currently, the global average marine reservoir age of surface waters, R(t), is c. 400 radiocarbon years; however, regional values deviate from this as a function of climate and oceanic circulation systems. These local deviations from R(t) are expressed as +R values. Hence, polar waters exhibit greater reservoir ages (δR = c. +400 to +800 <sup>14</sup>C y) than equatorial waters (δR = c. 0 <sup>14</sup>C y). Observed temporal variations in δR appear to reflect climatic and oceanographic changes. We assess three approaches to quantification of marine reservoir effects using known age samples (from museum collections), tephra isochrones (present onshore/offshore) and paired marine/terrestrial samples (from the same context in, for example, archaeological sites). The strengths and limitations of these approaches are evaluated using examples from the North Atlantic region. It is proposed that, with a suitable protocol, accelerator mass spectrometry (AMS) measurements on paired, short-lived, single entity marine and terrestrial samples from archaeological deposits is the most promising approach to constraining changes over at least the last 5 ky BP
Bortezomib decreases Rb phosphorylation and induces caspase-dependent apoptosis in Imatinib-sensitive and -resistant Bcr-Abl1-expressing cells
The use of c-abl-specific inhibitors such as Imatinib (IM)
or Dasatinib has revolutionized the treatment of chronic
myeloid leukemia (CML). However, a significant percentage
of patients become resistant to IM. In this report, we
have analyzed the possibility of using the proteasome as a
molecular target in CML. Our results show that cells that
express Bcr-Abl1 are more sensitive to the inhibition of
the proteasome with Bortezomib (Btz) than control cells.
This treatment reduces the proliferation of Bcr-Abl1-
expressing cells, by inactivating NF-jB2 and decreasing
the phosphorylation of Rb, eventually leading to an
increase in caspase-dependent apoptosis. Furthermore,
we show that Btz also induces cell-cycle arrest and
apoptosis in cells expressing Bcr-Abl1 mutants that are
resistant to IM. These results unravel a new molecular
target of Btz, that is the Rb pathway, and open new
possibilities in the treatment of CML especially for
patients that become resistant to IM because of the
presence of the T315I mutation
- …