SIMULTANEOUS EXTRACTION AND RAPID HPLC BASED QUANTIFICATION OF CROCIN AND SAFRANAL IN SAFFRON (CROCUS SATIVUS L.)

Objective: Saffron (Crocus sativus L.) is one of the most valuable crops with high medicinal values used in many diseases. The aim of the study was to establish the reliability and rapid HPLC method for analysis of crocin and safranal concentration present in stigmata of saffron in the market as well as field grown samples.Methods: Field grown and market stigmata of saffron were used for extraction of crocin and safranal. The linear dynamic ranges were established after validating the robustness of critical method parameters. The Agilent1260-Infinity Quaternary LC system was used for the preparation of calibration standards and quantification of crocin and safranal in C. sativus stigmata.Results: A good linearity was achieved in the range of 10-30 µg for each compound with the determination coefficient (R2). The calibration curves revealed linear regression (r. 0.997) for this rapid HPLC method and limit of quantifications (LOQs) were achieved in the range of 3.4 µg/ml for crocin and 10.2 µg/ml for safranal. The limit of detection (LODs) for all standards was ≤4.2 µg/ml. The range of crocin content (10.43-16.32 mg/g) and safranal (5.19-5.21 mg/g) was estimated in saffron samples.Conclusion: This method may serve the purpose of accurate quantification of crocin and safranal present in the stigmata of the plant in a quick time period.Keywords: Saffron, Crocin, Safranal, Extraction, Quantification, HPL

Experimental assessment and modeling of solar air heater with V shape roughness on absorber plate

A roughness of the absorber plate can improve the efficiency of a solar air heater. To boost the efficiency of triangle solar air heaters, this research presents the results of a comparison study between with and without rib roughness on absorber plates. Both use black paint with graphene nanoparticles infused into it, coating an absorber plate. Both numerical and experimental methods have been used to examine the impact of surface roughness on friction factors and heat transport properties. ANSYS 14.5 software module and RNG turbulence, k-€ model is used to conduct a three-dimensional simulation and solve the governing equations in the turbulent situation. Based on experimental data, it has been established that smooth plates are more efficient in converting heat into useful work than rough ones, on average, by a factor of 4.82 and 4.46, respectively. The length of the duct in the solar air heater mitigates the temperature gradient seen in the simulation result. The roughness of V-shaped ribs has a far larger effect on the heat transfer and friction factor properties than do variations in relative roughness pitch (P/e) and Reynolds number (Re). Experimental observations supported by modeling and simulation confirms that triangular duct absorber surface roughness provides improved outcome

Correlation of Circulating ANGPTL5 Levels With Obesity, High Sensitivity C-Reactive Protein and Oxidized Low Density Lipoprotein in Adolescents

Angiopoietin-like proteins (ANGPTL) is a family of eight members known to play an important role in metabolic diseases. Of these, ANGPTL5 is suggested to regulate triglyceride metabolism and is increased in obesity and diabetes. However, its role in metabolic diseases in adolescents is not well-studied. In this study, we tested the hypothesis of a positive association between plasma ANGPTL5, and obesity, high sensitivity C-reactive protein (HsCRP) and oxidized low-density lipoprotein (Ox-LDL) in adolescents. Adolescents (N = 431; age 11–14 years) were randomly selected from middle schools in Kuwait. Obesity was classified by the BMI-for-age based on the WHO growth charts. Plasma ANGPTL5, HsCRP, and Ox-LDL were measured using ELISA. The prevalence of overweight and obesity was 20.65% and 33.18%, respectively. Mean (SD) plasma ANGPTL5 levels were significantly higher in obese, compared with overweight and normal-weight adolescents (23.05 (8.79) vs 18.39 (7.08) ng/mL, and 18.26 (6.95) ng/ml, respectively). ANGPTL5 was positively associated with both HsCRP (ρ=0.27, p \u3c 0.001) and Ox-LDL (ρ = 0.24, p \u3c 0.001). In Conclusion, ANGPTL5 levels are elevated in obese adolescents and are associated with cardiovascular disease risk factors, HsCRP and Ox-LDL. The use of ANGPTL5 as a powerful diagnostic and prognostic tool in obesity and metabolic diseases needs to be further evaluated

Urocortin Neuropeptide Levels Are Impaired in the PBMCs of Overweight Children

The corticotropin-releasing hormone (CRH) and urocortins (UCNs) have been implicated in energy homeostasis and the cellular stress response. However, the expression of these neuropeptides in children remains unclear. Therefore, we determined the impact of obesity on their expression in 40 children who were normal weight, overweight, and had obesity. Peripheral blood mononuclear cells (PBMCs) and plasma were used to assess the expression of neuropeptides. THP1 cells were treated with 25 mM glucose and 200 µM palmitate, and gene expression was measured by real-time polymerase chain reaction (RT-PCR). Transcript levels of neuropeptides were decreased in PBMCs from children with increased body mass index as indicated by a significant decrease in UCN1, UCN3, and CRH mRNA in overweight and obese children. UCN3 mRNA expression was strongly correlated with UCN1, UCN2, and CRH. Exposure of THP1 cells to palmitate or a combination of high glucose and palmitate for 24 h increased CRH, UCN2, and UCN3 mRNA expression with concomitant increased levels of inflammatory and endoplasmic reticulum stress markers, suggesting a crosstalk between these neuropeptides and the cellular stress response. The differential impairment of the transcript levels of CRH and UCNs in PBMCs from overweight and obese children highlights their involvement in obesity-related metabolic and cellular stress

Burnout among surgeons before and during the SARS-CoV-2 pandemic: an international survey

Background: SARS-CoV-2 pandemic has had many significant impacts within the surgical realm, and surgeons have been obligated to reconsider almost every aspect of daily clinical practice. Methods: This is a cross-sectional study reported in compliance with the CHERRIES guidelines and conducted through an online platform from June 14th to July 15th, 2020. The primary outcome was the burden of burnout during the pandemic indicated by the validated Shirom-Melamed Burnout Measure. Results: Nine hundred fifty-four surgeons completed the survey. The median length of practice was 10 years; 78.2% included were male with a median age of 37 years old, 39.5% were consultants, 68.9% were general surgeons, and 55.7% were affiliated with an academic institution. Overall, there was a significant increase in the mean burnout score during the pandemic; longer years of practice and older age were significantly associated with less burnout. There were significant reductions in the median number of outpatient visits, operated cases, on-call hours, emergency visits, and research work, so, 48.2% of respondents felt that the training resources were insufficient. The majority (81.3%) of respondents reported that their hospitals were included in the management of COVID-19, 66.5% felt their roles had been minimized; 41% were asked to assist in non-surgical medical practices, and 37.6% of respondents were included in COVID-19 management. Conclusions: There was a significant burnout among trainees. Almost all aspects of clinical and research activities were affected with a significant reduction in the volume of research, outpatient clinic visits, surgical procedures, on-call hours, and emergency cases hindering the training. Trial registration: The study was registered on clicaltrials.gov "NCT04433286" on 16/06/2020

Arbitral awards of the Cairo Regional Centre for International Commercial Arbitration

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An assessment of mechanical vs manual harvesting of the sugarcane in Sudan – The case of Sennar Sugar Factory

The rehabilitation works carried out in the Sennar sugarcane factory in Sudan, improved the rates of milling accompanied by the horizontal and vertical expansions of the farm compelled Sennar factory to go for mechanical harvesting to solve the problem of labor shortage. The aim of this paper is to evaluate the current sugarcane manual and mechanical harvesting systems with regard to production efficiency, cost effectiveness, cane loading efficiency, infield losses, and the effect of trash (extraneous matter) in factory process. Different experiments were conducted to compare and contrast between the two harvesting systems. The results revealed that manual harvesting (8.98 SDG/ton) is more expensive than mechanical harvesting (4.9.5 SDG/ton); the wages for the cane cutting labor represent 74.14% of the total cutting cost, 46% of the total manual harvesting cost, and 18.9% of the total harvesting cost. Infield cane losses represent 4.72% and 4.22% of the actual yield for the manual harvesting and mechanical harvesting systems, respectively. Moreover, the results showed a significant difference between the two harvesting systems with regard to the cane weight/trailer (ton/trailer) and trash percent. On average the cane weight (ton/trailer) is 6.88 for manual harvesting and 10.12 for mechanical harvesting. The trash percent is only 3.66% for manually harvested cane while it reached 9.49 for the mechanically harvested cane. A 1% increase in trash will lead to decrease in sugar recovery by 0.1%. It could recommended that Sennar Sugar Factory as well as other sugar factories in Sudan could go for increasing the mechanical harvesting system and reducing the manual harvesting to less than 10% of the total area. The specialized cane cutter labors should be employed in other agricultural operations during the off-season to insure their availability at the start of the season. A further research is needed to reduce the infield cane losses and trash percent and to determine their effect on cane quality and factory performance for the two harvesting systems