High-throughput screening of inhibitory effect of medicinal plants on bacterial DNA polymerases

European Biotechnology Conference -- MAY 05-07, 2016 -- LATVIAWOS: 000380240300080[No abstract available

Gram (-) microorganisms DNA polymerase inhibition, antibacterial and chemical properties of fruit and leaf extracts of Sorbus acuparia and Sorbus caucasica var. yaltirikii

BELDUZ, Ali Osman/0000-0003-2240-7568; k, selvi/0000-0002-9912-8586; SANDALLI, Cemal/0000-0002-1298-3687WOS: 000400604700017PubMed: 27859484Investigation of novel plant-based agents might provide alternative antibiotics and thus fight antibiotic resistance. Here, we measured the ability of fruit and leaf extracts of Sorbus aucuparia (Sauc) and endemic Sorbus caucasica var. yaltirikii (Scau) to inhibit nonreplicative (Klenow Fragment-KF and Bacillus Large Fragment-BLF) and replicative (DnaE and PolC) bacterial DNA polymerases along with their antimicrobial, DPPH free radical scavenging activity (RSA), and chemical contents by total phenolic content and HPLC-DAD analysis. We found that leaf extracts had nearly 10-fold higher RSA and 5-fold greater TPC than the corresponding fruit extracts. All extracts had large amounts of chlorogenic acid (CGA) and rutin, while fruit extracts had large amounts of quercetin. Hydrolysis of fruit extracts revealed mainly caffeic acid from CGA (caffeoylquinic acid) and quercetin from rutin (quercetin-3-O-rutinoside), as well as CGA and derivatives of CGA and p-coumaric acid. Plant extracts of Sorbus species showed antimicrobial activity against Gram-negative microorganisms. Scau leaf extracts exhibited strong inhibition of KF activity. Sauc and Scau leaf extracts also strongly inhibited two replicative DNA polymerases. Thus, these species can be considered a potential source of novel antimicrobial agents specific for Gram-negative bacteria.Scientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-113Z054]The Scientific and Technical Research Council of Turkey, Grant/Award Number: TUBITAK-113Z05

Effect of thermocycling on the amount of monomer released from bulk fill composite resins

Hatipoglu, Omer/0000-0002-4628-8551; Karadas, Muhammet/0000-0002-3357-6896WOS: 000510806600019PubMed: 31582597The goal of this study was to examine the effect of thermal cycling on the amount of monomer released from bulk fill composites. Five bulk fill composite resins were used in the study. Extraction solutions were obtained at the end of the time/thermal cycle periods: 0-1 day/0-1,500, 1-3 days/1,500-4,500 and 3-7 days/4,500-10,000. the monomers in the extractions samples taken at each time point were measured on an HPLC instrument. the obtained data were analyzed by repeated measures of variance analysis and tukey multiple comparison tests (p<0.05). the thermocycling increased the amount of monomer released from all composites at 0-1 day (p<0.05). At 0-1 and 1-3 days, Venus Bulk Fill and Filtek Bulk Fill composite resins were more affected. Polymer networks with high molecular weight monomers such as Bis-GMA and UDMA can be less affected by thermal changes compared to polymers with low molecular weight monomers.Recep Tayyip Erdogan University Research FundRecep Tayyip Erdogan University [2015.53001.111.03.01]The data presented in this study are a part of the doctoral thesis of Mr. Omer HATIPOGLU. This study was supported by Recep Tayyip Erdogan University Research Fund under Grant (Project code: 2015.53001.111.03.01)

Influence of different light-curing units on monomer elution from bulk fill composites

Hatipoglu, Omer/0000-0002-4628-8551; Karadas, Muhammet/0000-0002-3357-6896WOS: 000456292100008Objectives: This in vitro study assessed the effect of different light-curing units on the elution of monomers from bulk fill composites with different thicknesses. Methods: Five bulk fill composites (Filtek Bulk Fill Flowable, SonicFill 2, SDR, Tetric N-Ceram Bulk Fill, and Venus Bulk Fill) and one conventional composite (Filtek Z250) were selected for the study. the cylindrical specimens in thickness of 2 mm or 4 mm were prepared and photopolymerized for 20 s with a light-emitting diode (VALO Cordless) or a halogen (Monitex BlueLuxer) light-curing unit. the specimens in glass vials were covered with a 75% ethanol/water solution. Ethanol/water extraction solutions were collected for high-performance liquid chromatography analysis after 24 h, 3 days, and 7 days. the data were analyzed with repeated measures and three-way ANOVA (p < 0.05). Results: the total monomer amount was significantly influenced by light-curing source used and thickness. the highest levels of Bis-GMA and Bis-EMA were eluted from Tetric N-Ceram BulkFill and Venus Bulk Fill, respectively. SonicFill 2 released the highest level of TEGDMA at 4-mm thickness. the highest levels of UDMA release, from 4-mm-thick Filtek Bulk Fill Flowable, were attained using the halogen unit. Conclusions: Light-curing sources affected the number of monomers released by materials. the amount of eluted monomers declined over time. the increased ratio of released monomers to increased thickness is material dependent. the number of residual monomers is highly associated with the resin ratio and crosslinking network of the composites.Recep Tayyip Erdogan University Research FundRecep Tayyip Erdogan University [2015.53001.111.03.01]1 This study was supported by Recep Tayyip Erdogan University Research Fund under Grant (Project code: [2015.53001.111.03.01])

Paeoniflorigenone purified from Paeonia daurica roots potently inhibits viral and bacterial DNA polymerases: investigation by experimental validation and docking simulation

emirik, mustafa/0000-0001-9489-9093WOS: 000496324100012The methanolic extracts from fruit, leaf, stem and roots of Paeonia daurica subsp. macrophylla (P. daurica) were investigated for inhibitory effect on replicative bacterial (PolC and DnaE2) and viral (MMLV-RT from Moloney Murine Leukemia Virus) DNA polymerases by primer extension assay. While all plant parts showed inhibition effect on bacterial and viral DNA polymerases, roots of the plant was focused to purify inhibitory compound(s). the chemical structures of compounds were completely elucidated using a combination of NMR, MS and FT-IR analyses. Five molecules with tree monoterpene glycosides, paeoniflorin (PD-2), paeoniflorigenone (PD-4), benzoyl paeoniflorin (PD-5), and benzoic acid (PD-3) with its derivate 2,4,6-trihydroxy-1-methyl benzoate (PD-1) were purified and identified. Both DNAdependent and RNA-dependent polymerase activity of MMLV-RT was strongly inhibited by these five molecules. on the other hand, bacterial polymerases PolC and DnaE2 were strongly inhibited by only paeoniflorigenone (PD-4). Molecular modeling result suggested that paeoniflorigenone (PD-4) interacts with the important residues at active site (palm, fingers and thumb domains) of three polymerases which support our experimental result. Ethyl acetate fraction had smallest SC50 value against DPPH and ABTS radicals. It showed also higher scavenging activity than quercetin, trolox and ascorbic acid since its quite high total phenolic content. We proposed that the parts of P. daurica might be used to find new antimicrobial agents and generate supplementary material for foods. Furthermore, the isolated molecules with inhibitory effect may be used as new scaffold for the further modification in order to develop novel inhibitors against DNA polymerization

An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles

This study investigated some physico-chemical and biochemical characteristics of different honey types belonging to Turkish flora. Sixty-two honey samples were examined on the basis of pollen analyses, including 11 unifloral honeys (chestnut, heather, chaste tree, rhododendron, common eryngo, lavender, Jerusalem tea, astragalus, clover and acacia), two different honeydew honeys (lime and oak), and 7 different multifloral honeys. Electrical conductivity, moisture, Hunter color values, HMF, proline, diastase number, and sugar analyses of the honey samples were assessed for chemical characterization. Some phenolic components were analyzed by reverse phase high performance liquid chromatography (RP-HPLC) to determine honeys' phenolic profiles. Total phenolic compounds, total flavonoids, ferric reducing antioxidant capacity (FRAP) and 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity were measured as antioxidant determinants. The study results confirm that physico-chemical and biological characteristics of honeys are closely related to their floral sources, and that dark-colored honeys such as oak, chestnut and heather, have a high therapeutic potential. (C) 2015 Elsevier Ltd. All rights reserved

Grayanotoxin-III Detection and Antioxidant Activity of Mad Honey

Sahin, Huseyin/0000-0002-6018-1494WOS: 000359160500006Mad honey is a complex mixture of numerous chemicals produced by honeybees from Rhododendron flowers. Consumption of mad honey leads to diarrhea, perspiration, dizziness, changes in consciousness, syncope, diplopia, as well as blurred vision, hypotension, and bradycardia due to the presence of grayanotoxins (GTXs). Therefore, it is important to detect the level grayanotoxins in mad honey. Besides its toxicity, mad honey also has antioxidant activity. This study was designed to determine the level of grayanotoxin-III toxin and antioxidant activity of ten different mad honey samples collected from the Black Sea region of Turkey. Liquid chromatography-tandem mass spectrometry was used for the quantitation of grayanotoxin-III. Antioxidant activity was evaluated using total phenolic contents, total ferric reducing antioxidant power, scavenging of 2,2-diphenyl-1-picrylhydrazyl and 3-(2-pyridyl)-5, 6-diphenyl-1,2,4-triazine-4',4''-disulfonic acid radicals. Quantities of grayanotoxin-III levels ranged from 68.754 to 0.701 mu g grayanotoxin-III/g honey. Mad honey MH7 from Artvin/Hopa had the highest grayanotoxin-III level. Although there were varying levels of grayanotoxin-III, mad honey samples were outstanding in terms of antioxidant activity. MH3 had the highest antioxidant potential. Although toxicity effect comprises, a metered dose of mad honey might also be explored as a potential source in clinical trials due to high bioactivity levels.TAGEMGida Tarim Ve Hayvancilik Bakanligi [AR-GE/15]; Tubitak BidebTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)The study was supported by TAGEM AR-GE/15, and author Huseyin Sahin was supported by a grant from the research fund of Tubitak Bideb for his Ph.D. research

Effect of ethyl acetate extract of usnea longissima on esophagogastric adenocarcinoma in rats

Cetin, Nihal/0000-0003-3233-8009; yilmaz, adnan/0000-0003-4842-1173WOS: 000461476800004PubMed: 30892391Purpose: To investigate the effects of the EtOAc extract of U. longissima which is uninvestigated previously on esophagogastric cancer induced in rats with N-methyl-N-nitro-N-nitrosoguanidin (MNNG). Methods: the anticancer activity of EtOAc extract of U. longissima was examined in the esophagogastric adenocarcinoma models induced in rats with MNNG. EtOAc extract of U. longissima, 50 and 100 mg/kg oral doses were administered once daily for six months. MNNG induced differentiated and undifferentiated type adenocarcinomas in the esophageal and gastric tissues of rats. Results: EtOAc extract of U. longissima obtained from U. longissima prevented gastric and esophageal cancerogenesis induced in rats with MNNG. EtOAc extract of U. longissima did not have a lethal effect at doses of 500, 1000 and 2000 mg/kg. the prominent anticarcinogenic activity of EtOAc extract of U. longissima 50 and 100 mg/kg suggests that it is not toxic and it is selective to the cancer tissue. Conclusion: This information may shed light on clinical implementation of EtOAc extract of U. longissima in future