China and the crisis : global power, domestic caution and local initiative

Even though the global crisis had a quick and dramatic impact on Chinese exports, the Chinese government responded with a range of policy responses that have helped maintain high rates of growth. This success has helped propel China to the centre of global politics, accelerating what many perceive to be a power shift from the West to China. But these gains were achieved by reversing policy in previous years designed to make a fundamental shift in China‟s mode of development, and have highlighted the problems associated with making such a transition. At the moment that many are looking at the Chinese "model" as a potential alternative to the Washington Consensus, one of the consequences of the crisis is to further question the long term efficacy of this "model" in China itself

Isospin structure of one- and two-phonon GDR excitations

Isospin is included in the description of Coulomb excitation of multiple giant isovector dipole resonances. In the excitation of even-even nuclei, a relevant portion of the excitation strength is shown to be associated with 1+ two-phonon states, which tends to be hindered or completely supressed in calculations in which the isospin degree of freedom is not considered. We find that the excitation cross sections is strongly dependent on the ground state isospin.Comment: 8 pages, 2 figure

Pharmacological interventions enhance virus-free generation of TRAC-replaced CAR T cells

Chimeric Antigen Receptor (CAR) redirected T-cells are potent therapeutic options against hematological malignancies. The current dominant manufacturing approach for CAR T cells depends on retroviral transduction. With the advent of gene editing, insertion of a CD19-CAR into the T cell receptor (TCR) alpha constant (TRAC) locus using adeno-associated viruses for gene transfer was demonstrated, and these CD19-CAR T-cells showed improved functionality over their retrovirally transduced counterparts. However, clinical-grade production of viruses is complex and associated with extensive costs. Here, we optimized a virus-free genome editing method for efficient CAR insertion into the TRAC locus of primary human T-cells via nuclease-assisted homology-directed repair (HDR) using CRISPR-Cas and double-stranded template DNA (dsDNA). We evaluated DNA-sensor inhibition and HDR enhancement as two pharmacological interventions to improve cell viability and relative CAR knock-in rates, respectively. While the toxicity of transfected dsDNA was not fully prevented, the combination of both interventions significantly increased CAR knock-in rates and CAR T-cell yield. Resulting TRAC-replaced CD19-CAR T-cells showed antigen-specific cytotoxicity and cytokine production in vitro and slowed leukemia progression in a xenograft mouse model. Amplicon-sequencing did not reveal significant indel formation at potential off-target sites with or without exposure to DNA-repair modulating small molecules. With TRAC-integrated CAR+ T-cell frequencies exceeding 50%, this study opens new perspectives to exploit pharmacological interventions to improve non-viral gene editing in T-cells

Paleoseismic History of the Dead Sea Fault Zone

International audienceThe aim of this entry is to describe the DSF as a transform plate boundary pointing out the rate of activedeformation, fault segmentation, and geometrical complexities as a control of earthquake ruptures. Thedistribution of large historical earthquakes from a revisited seismicity catalogue using detailedmacroseismic maps allows the correlation between the location of past earthquakes and fault segments.The recent results of paleoearthquake investigations (paleoseismic and archeoseismic) with a recurrenceinterval of large events and long-term slip rate are presented and discussed along with the identification ofseismic gaps along the fault. Finally, the implications for the seismic hazard assessment are also discussed

A search for very high energy gamma-ray emission from the starburst galaxy NGC 253 with H.E.S.S

We present the result of 28 hours of observations of the nearby starburst galaxy NGC 253 with the H.E.S.S. detector in 2003. We find no evidence for very high energy gamma-ray emission from this object. Gamma-ray emission above 400 GeV from NGC 253 had been reported by the CANGAROO collaboration in 2002. From the H.E.S.S. data we derive upper limits on the flux above 300 GeV of 1.9 * 10^-12 photons cm^-2 s^-1 for a point-like source and 6.3 * 10^-12 photons cm^-2 s^-1 for a source of radius 0.5 degrees as reported by CANGAROO, both at a confidence level of 99%. These upper limits are inconsistent with the spectrum reported by CANGAROO. The expected very high energy gamma-ray emission from this object is discussed in the framework of a galactic wind propagation model.Comment: 7 pages, 3 figure

Fermi Gamma-ray Space Telescope: High-Energy Results from the First Year

The Fermi Gamma-ray Space Telescope (Fermi) was launched on June 11, 2008 and began its first year sky survey on August 11, 2008. The Large Area Telescope (LAT), a wide field-of-view pair-conversion telescope covering the energy range from 20 MeV to more than 300 GeV, is the primary instrument on Fermi. While this review focuses on results obtained with the LAT, the Gamma-ray Burst Monitor (GBM) complements the LAT in its observations of transient sources and is sensitive to X-rays and gamma-rays with energies between 8 keV and 40 MeV. During the first year in orbit, the Fermi LAT has observed a large number of sources that include active galaxies, pulsars, compact binaries, globular clusters, supernova remnants, as well as the Sun, the Moon and the Earth. The GBM and LAT together have uncovered surprising characteristics in the high-energy emission of gamma-ray bursts (GRBs) that have been used to set significant new limits on violations of Lorentz invariance. The Fermi LAT has also made important new measurements of the Galactic diffuse radiation and has made precise measurements of the spectrum of cosmic-ray electrons and positrons from 20 GeV to 1 TeV.Comment: 39 pages, 16 figure

Spawning of bluefin tuna in the black sea: historical evidence, environmental constraints and population plasticity

<div><p>The lucrative and highly migratory Atlantic bluefin tuna, <em>Thunnus thynnus</em> (Linnaeus 1758<em>;</em> Scombridae), used to be distributed widely throughout the north Atlantic Ocean, Mediterranean Sea and Black Sea. Its migrations have supported sustainable fisheries and impacted local cultures since antiquity, but its biogeographic range has contracted since the 1950s. Most recently, the species disappeared from the Black Sea in the late 1980s and has not yet recovered. Reasons for the Black Sea disappearance, and the species-wide range contraction, are unclear. However bluefin tuna formerly foraged and possibly spawned in the Black Sea. Loss of a locally-reproducing population would represent a decline in population richness, and an increase in species vulnerability to perturbations such as exploitation and environmental change. Here we identify the main genetic and phenotypic adaptations that the population must have (had) in order to reproduce successfully in the specific hydrographic (estuarine) conditions of the Black Sea. By comparing hydrographic conditions in spawning areas of the three species of bluefin tunas, and applying a mechanistic model of egg buoyancy and sinking rate, we show that reproduction in the Black Sea must have required specific adaptations of egg buoyancy, fertilisation and development for reproductive success. Such adaptations by local populations of marine fish species spawning in estuarine areas are common as is evident from a meta-analysis of egg buoyancy data from 16 species of fish. We conclude that these adaptations would have been necessary for successful local reproduction by bluefin tuna in the Black Sea, and that a locally-adapted reproducing population may have disappeared. Recovery of bluefin tuna in the Black Sea, either for spawning or foraging, will occur fastest if any remaining locally adapted individuals are allowed to survive, and by conservation and recovery of depleted Mediterranean populations which could through time re-establish local Black Sea spawning and foraging.</p> </div