Comparative Analysis of Apoptotic Resistance of Mesenchymal Stem Cells Isolated from Human Bone Marrow and Adipose Tissue

Aim. Mesenchymal stem cells (MSCs) isolated from human bone marrow (hBM) and adipose tissue (hAT) are perceived as attractive sources of stem cells for cell therapy. The aim of this study was to compare MSCs from hBM and hAT for their immunocytochemistry staining and resistance to in vitro apoptosis. Methods. In our study, we investigated the antiapoptotic ability of these MSCs toward oxidative stress induced by hydrogen peroxide (H2O2) and serum deprivation. Results were assessed by MTT and flow cytometry. All experiments were repeated a minimum of three times. Results. Flow cytometry and MTT analysis revealed that hAT-MSCs exhibited a higher resistance toward H2O2-induced apoptosis (n = 3, hBM-hAT viability H2O2  58.43 ± 1.24–73.02 ± 1.44, P < 0.02) and to serum-deprivation-induced apoptosis at days 1 and 4 than the hBM-MSCs (n = 3, hAT-hBM absorbance, resp., day 1: 0.305 ± 0.027–0.234 ± 0.015, P = 0.029, day 4: 0.355 ± 0.003–0.318 ± 0.007, P = 0.001, and day 7: 0.400 ± 0.017–0.356 ± 0.008, P = 0.672). hAT-MSCs showed superior tolerance to oxidative stress triggered by 2 mmol/L H2O2 and also have superior antiapoptosis capacity toward serum-free culture. Conclusion. In this study we found that hAT-MSCs are more resistant to in vitro apoptosis

DYKE-DAVIDOFF-MASSON SYNDROME: MYOCLONIC SEIZURES AND HEMIHYPERTROPHY IN LATE CHILDHOOD: A CASE REPORT

Dyke-Davidoff-Masson Syndrome (DDMS) is a rare syndrome characterized with specific clinical and radiological findings due to involvement of the developing brain with cerebral hemiatrophy of one hemisphere. The syndrome was first described from Dyke, Davidoff and Masson in 1933 in a series of nine patients. Syndrome has two forms, congenital and acquired forms and etiological factors vary due to involvement of the brain. Most common clinical symptom are focal or secondary generalized seizures. Hemiparesis, facial asymmetry, intellectual disability, mental retardation, and hemihypertrophy also seen in clinical process. In magnetic resonance imaging (MRI) DDMS has unique radiological findings. Seizures are commonly refractory to treatment and aim of the treatment is to control seizures and improve mental and intellectual capabilities. Prognosis is good when clinical findings occur after two years old

Enflasyon hedeflemesi rejimi: Türkiye örneği

Bu çalışmada enflasyon hedeflemesi rejimi en temel kavramları ile aktarılmış olup, Türkiye’nin gelişmiş ve diğer gelişmekte olan ülkeler ile karşılaştırılması yapılmaya çalışılmıştır. İlk bölümde genel olarak para politikalarından bahsedilmiştir. Enflasyon hedeflemesi rejimi politikasını açıklamak adına avantaj ve dezavantajları gösterilmektedir. Makalenin ikinci bölümünde ise gelişmiş ve gelişmekte olan ülkelerin enflasyon hedeflemesi rejimi tarihlerine değinilmiş olup rejimin enflasyon oranları üzerindeki etkileri ele alınmıştır. Üçüncü bölüme gelindiğinde ise Türkiye’nin enflasyon tarihi ele alınmış ve 2002-2005 yıllarında örtük enflasyon hedeflemesi ile geçiş yaptığı enflasyon hedeflemesi rejiminden sonraki enflasyonist sürecine vurgu yapılmıştır. Genel değerlendirme çerçevesinde ise Türkiye’nin enflasyon hedeflemesi uygulamasında başarıya ulaşıp ulaşmadığı değerlendirilmiştir

Recovery of fertility in azoospermia rats after injection of adipose-tissue-derived mesenchymal stem cells: the sperm generation

The recent reports on the treatment of azoospermia patients, in which spermatozoa could not be traced in their testes, are focused more on the potential use of adult stem cells, like mesenchymal stem cells (MSCs). The aim of this study was to demonstrate the potential use of MSCs derived from adipose tissue in the treatment of azoospermia using rat disease models. After left rete testes. After 12 weeks, the testes with cell injection (right testes) were compared to control (left testes) after dimensional and immunohistochemical analyses. Testes treated with MSCs appeared morphologically normal, but they were atrophic in rats without stem cell treatment, in which the seminiferous tubules were empty. Spermatogenesis was detected, not in every but in some tubules of cell-treated testes. GFP(+)/VASA(+) and GFP(+)/SCP1(+) cells in testes indicated the transdifferentiation of MSCs into spermatogenetic cells in the appropriate microenvironment. Rats with cell treatment were mated to show the full recovery of spermatogenesis, and continuous generations were obtained. The expression of GFP was detected in the mesenchymal stem cells derived from adipose tissue and bone marrow and also in the sperms of offspring. In conclusion, MSCs might be studied for the same purpose in humans in future.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110S506

Varicose veins during pregnancy: Risk factors and impact on quality of life

Objective: This study was carried out to examine the risk factors for varicose veins during pregnancy and their impact on quality of life. Material and methods: This was a prospective, cross-sectional observational study in pregnant women in their second and third trimesters. In addition to the collection of sociodemographic and lifestyle data, the presence of varicose veins was assessed using the clinical, etiological, anatomical, and pathophysiological (CEAP) questionnaire, and the quality of life was assessed using the Chronic Venous Insufficiency Questionnaire (CIVIQ-20). Logistic regression analysis was conducted to identify independent risk factors for varicose veins. Results: A total of 658 women were included. Considering all types of varicose veins, prevalence of varicose veins was 29% (191 women). Varicose veins presence was found to be significantly associated with gestational week (odd ratio [OR], 1.047; 95% CI, 1.013-1.083; P=0.03), thyroid diseases (OR, 2.474; 95% CI, 1.109-5.522; P=0.019), smoking status during pregnancy (OR, 7.294; 95% CI, 2.408-22.093; P<0.001), and positive family varix history (OR, 213.437; 95% CI, 87.248-522.138; P<0.001). As regards the quality-of-life evaluation, scores in all CIVIQ-20 dimensions-physical (mean deviation [MD], -4.30; 95% CI, -4.76 to 3.83; P<0.001), psychological (MD, -8.67; 95% CI, -9.60 to 7.73; P<0.001), social (MD, -3.13; 95% CI, -3.48 to 2.79; P<0.001), pain (MD, -3.94; 95% CI, -4.37 to 3.51; P<0.001)-and the global index score (MD, 25.06; 95% CI, 22.50 to 27.62; P<0.001) were significantly higher in patients with varicose veins than in those without (P<0.001). Conclusions: In this prospective, observational study in pregnant women, gestational week, thyroid diseases, smoking status during pregnancy, and positive family history were identified as risk factors for varicose veins, and the presence of varicose veins was found to negatively impact quality of life in this setting

Comparative Proteome Analysis of hAT-MSCs Isolated from Chronic Renal Failure Patients with Differences in Their Bone Turnover Status.

The relationship between the stem cells and the bone turnover in uremic bone disease due to chronic renal failure (CRF) is not described. The aim of this study was to investigate the effect of bone turnover status on stem cell properties. To search for the presence of such link and shed some light on stem-cell relevant mechanisms of bone turnover, we carried out a study with mesenchymal stem cells. Tissue biopsies were taken from the abdominal subcutaneous adipose tissue of a CRF patient with secondary hyperparathyroidism with the high turnover bone disease. This patient underwent parathyroidectomy operation (PTX) and another sample was taken from this patient after PTX. A CRF patient with adynamic bone disease with low turnover and a healthy control were also included. Mesenchymal stem cells isolated from the subjects were analyzed using proteomic and molecular approaches. Except ALP activity, the bone turnover status did not affect common stem cell properties. However, detailed proteome analysis revealed the presence of regulated protein spots. A total of 32 protein spots were identified following 2D gel electrophoresis and MALDI-TOF/TOF analyzes. The identified proteins were classified into seven distinct groups and their potential relationship to bone turnover were discussed. Distinct protein expression patterns emerged in relation to the bone turnover status indicate a possible link between the stem cells and bone turnover in uremic bone disease due to CRF