Android-based application for monitoring the rehabilitation of patients with breast cancer

Hoy en día, el cáncer es una enfermedad muy común. Los pacientes necesitan mucho apoyo psicológico, una rutina terapéutica y un gran seguimiento por parte del personal sanitario. Acerca del cáncer de mama, éste tiene una alta probabilidad de ser superado en comparación con otros tipos de cáncer. Esta enfermedad conlleva, en muchos casos, la extirpación de la mama afectada, lo que requiere rehabilitación para recuperar la movilidad en la zona. En este proyecto, vamos a desarrollar una aplicación basada en Android para el teléfono móvil con el fin de ayudar a los médicos y pacientes en la rehabilitación desde casa. Con la ayuda de esta aplicación y algunos sensores que los pacientes tienen que colocar en sus brazos, será posible monitorizar los ejercicios y proporcionar una retroalimentación sobre el desempeño. Esto les ayuda a recuperarse evaluando el ejercicio inmediatamente. De esta manera, se pueden mostrar los errores en la ejecución de estos.Nowadays, cancer is a very common disease. Patients need a lot of psychological support, a routine and a lot of follow-up by healthcare personnel. Focusing more on breast cancer, it has a high probability of being overcome compared to other types of cancer. This disease leads, in some cases, in a breast removal, which leads to rehabilitation to recover mobility in the area. In this project, we are going to develop an Android-based application for the mobile phone to help doctors and patients in rehabilitation from home. With the help of this application and some sensors that patients have to place on their arms, it will be possible to monitor the exercises and to provide a feedback about the performance. This helps them to recover by evaluating the exercise immediately. In this way, errors in the execution of the exercises can be shown.Grado en Ingeniería Telemátic

Closing eyes during auditory memory retrieval modulates alpha rhythm but does not alter tau rhythm

Available online 20 April 2019The alpha power increase that occurs when the eyes are closed is one of the most well-known effects in human electrophysiology. In particular, previous psychological studies have investigated whether eye closure can boost memory performance under certain circumstances, providing contradictory evidence across sensory input modalities. Although alpha power is modulated during different phases of memory and these modulations are correlated with performance, few studies have reported on the relationship between eye closure, memory, and alpha-band power. The present study investigates the influence of eye closure while participants (n = 21) performed an auditory recognition memory task with spoken words during the recording of magnetoencephalography (MEG) data. Our results showed no evidence for a behavioural effect of eye closure in the performance of the task. In addition, electrophysiological responses to the stimuli showed the expected alpha event-related desynchronization (ERD) 0.5–1 s and a high-alpha/beta event-related synchronization (ERS) 1–2 s after word onset. The data showed the expected memory effect, i.e. remembered words elicited greater 10 Hz ERD than forgotten words in the brain regions typically associated with the language network, suggesting a modulation of tau rhythm. Eye closure modulated alpha rhythm only in posterior-parietal and occipital regions. The lack of interaction and the different localizations found for modulations of tau and classical alpha rhythms suggests that these rhythms play distinct functional roles in memory performance.This research was possible thanks to the support of the “Severo Ochoa Program for Centres/Units of Excellence in R&D” (SEV-2015-490). AB was supported by the Basque Government (Eusko Jaurlaritza) under the program “Ikertzaile ez doktoreen doktoretza-aurreko formakuntza programa” ( PRE_2015_2_0208), CCG was supported by the Spanish Ministry of Economy and Competitiveness through the Juan de la Cierva (IJCI- 2014-20821) and Ramon y Cajal (RYC-2017-21845) Fellowships

Three-Dimensional Characterization of a Coastal Mode-Water Eddy from Multiplatform Observations and a Data Reconstruction Method

Coastal mesoscale eddies are important oceanic structures partially responsible for regulating ocean-shelf exchanges. However, their description and characterization are challenging; observations are often too scarce for studying their physical properties and environmental impacts at the required spatio-temporal resolution. Therefore, models and data extrapolation methods are key tools for this purpose. Observations from high-frequency radar, one satellite and two gliders, are used here to better characterize the three-dimensional structure of a coastal mode-water eddy from a multiplatform approach in the southeastern Bay of Biscay in spring 2018. After the joint analysis of the observations, a three-dimensional data reconstruction method is applied to reconstruct the eddy current velocity field and estimate the associated water volume transport. The target eddy is detected by surface observations (high-frequency radar and satellite) for two weeks and presents similar dimensions and lifetimes as other eddies studied previously in the same location. However, this is the first time that the water column properties are also observed for this region, which depicts a mode-water eddy behavior, i.e., an uplift of the isopycnals in the near-surface and a downlift deeper in the water column. The reconstructed upper water column (1–100 m) eddy dynamics agree with the geostrophic dynamics observed by one of the gliders and result in cross-shelf inshore (offshore) volume transports between 0.04 (−0.01) and 0.15 (−0.11) Sv. The multiplatform data approach and the data reconstruction method are here highlighted as useful tools to characterize and three-dimensionally reconstruct coastal mesoscale processes in coastal areas.En prensa0,64

Buckwheat grains treated with microwave radiation: Impact on the techno-functional, thermal, structural, and rheological properties of flour

Producción CientíficaThe physical modification of buckwheat grain, a pseudocereal with great interest in gluten-free (GF) product development, using microwave-assisted hydrothermal treatment (MWT), was evaluated. Buckwheat grains were microwaved (8 min at 18W/g) at four moisture contents (MC): 13%, 20%, 25%, and 30%, maintained constant throughout the treatment using a hermetic container. The impact of MWT on the techno-functional and rheological properties of flour was significantly affected by the MC of the grains. The flour obtained from grains treated with 30% MC (MW-30) was the most modified, showing the highest water absorption capacity (+43%) and a dramatic reduction in its emulsifying activity to almost zero, indicating the loss of its protein functionality during treatment. Differential scanning calorimetry (DSC) revealed a delay in the gelatinisation temperature (+3.7 °C) with a significant reduction in the gelatinization enthalpy (−27%), which is compatible with a partial pre-gelatinization of the flour. In contrast, the lowest grain MC led to opposite effects on most of the flour properties measured. The most significant difference was its improved ability to form stable emulsions (ES) (+188%) and a significant, albeit moderate, increase in gel structural stability and tolerance to stress. Based on these results, MWT combined with the MC of grain during treatment may be a viable and effective alternative to modulate the techno-functional properties of buckwheat flour and improve its applicability in GF food formulations.Ministerio de Ciencia, Innovación y Universidades (project PID2019-110809RB-I00)Junta de Castilla y Leon - Fondo Europeo de Desarrollo Regional (project VA195P20

Microwave modification of quinoa grains at constant and varying water content modulates changes in structural and physico-chemical properties of the resulting flours

Producción CientíficaMicrowave-assisted heat moisture treatment (MWT) was applied to quinoa grains, a nutritious gluten-free pseudocereal of great interest in food product development, to achieve the physical modification of the quinoa flour. The effect of treating quinoa grains at different initial water contents (WC; 10%, 20%, and 30%) in two operational systems was compared: one open at atmospheric pressure and variable WC (V system), and the other in hermetic containers at constant WC (C system). The morphological structure of the obtained flours and their techno-functional, rheological, and thermal properties were evaluated. MWT proved to be effective in modifying these properties, the main effects probably being caused by protein denaturation and aggregation, and intragranular molecular rearrangements of starch, with disruption of short-range molecular order and even the partial collapse of starch granules in the samples treated at the highest WC. The greatest differences were observed for the 20 and 30% WC treated-samples, particularly when using C system, which increased their water absorption capacity and decreased their foaming, emulsion, and gel-forming capacities. Based on these results, the control of WC and its evolution during MWT of quinoa grains appears to be a viable and effective approach to adapt flour functionality to the needs of food production, allowing a wider range of flour properties depending on the MWT conditions.Ministerio de Ciencia e Innovación (PID2019- 110809RB-I00/AEI/10.1303/501100011033)Junta de Castilla y León/FEDER (VA195P20

Solid-phase synthesis of imprinted nanoparticles as artificial antibodies against the C-terminus of the cannabinoid CB1 receptor: exploring a viable alternative for bioanalysis

[EN]The production of artificial anti-CB1 antibodies in nanoparticle format is described using the solid-phase imprinting approach. Instead of whole protein imprinting, a linear C-terminus sequence of the receptor comprising 15 amino acids (458-KVTMSVSTDTSAEAL-472) has been used as template, in accordance with the epitope imprinting approach. This sequence is located intracellularly, and it is involved in coupling to G(i/o) proteins, being responsible for CB1 receptor desensitisation and internalisation. Developed molecularly imprinted materials were found to be in the nanometre scale, with a particle size of 126.4 +/- 10.5 nm at pH 3 (25 oC) and spherical shape. It was also observed that the size was sensible to temperature changes being reduced to 106.3 +/- 15.2 nm at 35 degrees C. Lower critical solution temperature of this polymer was found to be approximate to 33.4 degrees C. The affinity and selectivity of the artificial antibody were assessed through dot blot and Western blot experiments. For the latter, recombinant fusion proteins GST-CB1(414-472) and GST-CB1(414-442) were produced to work respectively as target and negative control proteins. The control protein did not carry the target epitope for being devoid of last 30 amino acids at the C-terminus. The results demonstrated that the anti-CB1 material recognised selectively the target protein, thanks to the presence of the 15-amino acid sequence selected as epitope, which revealed that binding occurred at the C-terminus of the receptor itself. The methodology presented may pave the way for the development of novel imprinted nanomaterials for other proteins included in the superfamily of the G-protein-coupled receptors (GPCR).Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funding for this research was provided by the Spanish Ministry of Science, Innovation and Universities (project CTQ2017-85686-R) and by the Basque Government (Research Groups of the Basque University System, Project No IT 1186-19

Related Factors of Anemia in Critically Ill Patients: A Prospective Multicenter Study

Anemia; Blood; Practice managementAnemia; Sangre; Gestión prácticaAnèmia; Sang; Gestió pràcticaAnemia is common in critically ill patients; almost 95% of patients admitted to intensive care units (ICUs) have hemoglobin levels below normal. Several causes may explain this phenomenon as well as the tendency to transfuse patients without adequate cause: due to a lack of adherence to protocols, lack of supervision, incomplete transfusion request forms, or a lack of knowledge about the indications, risks, and costs of transfusions. Daily sampling to monitor the coagulation parameters and the acid–base balance can aggravate anemia as the main iatrogenic factor in its production. We studied the association and importance of iatrogenic blood loss and other factors in the incidence of anemia in ICUs. We performed a prospective, observational, multicenter study in five Spanish hospitals. A total of 142 patients with a median age of 58 years (IQI: 48–69), 71.83% male and 28.17% female, were admitted to ICUs without a diagnosis of iatrogenic anemia. During their ICU stay, anemia appeared in 66.90% of the sample, 95 patients, (95% CI: 58.51–74.56%). Risk factors associated with the occurrence of iatrogenic anemia were arterial catheter insertion (72.63% vs. 46.81%, p-value = 0.003), venous catheter insertion (87.37% vs. 72.34%, p-value = 0.023), drainages (33.68% vs. 12. 77%, p-value = 0.038), and ICU stay, where the longer the stay, the higher the rate of iatrogenic anemia (p-value < 0.001). We concluded that there was a statistical significance in the production of iatrogenic anemia due to the daily sampling for laboratory monitoring and critical procedures in intensive care units. The implementation of patient blood management programs could address these issues

Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

Background: Replacement of radioligand binding assays with antibody-antigen interaction-based approaches for quantitative analysis of G protein-coupled receptor (GPCR) levels requires the use of purified protein standards containing the antigen. GPCRs in general and cannabinoid CB1 receptor in particular show a progressive tendency to aggregate and precipitate in aqueous solution outside of their biological context due to the low solubility that the hydrophobic nature imprinted by their seven transmembrane domains. This renders full-length recombinant GPCRs useless for analytical purposes, a problem that can be overcome by engineering soluble recombinant fragments of the receptor containing the antigen. Results: Here we generated highly soluble and stable recombinant protein constructs GST-CB1(41)(4-)(472 )and GST-CB1(414-442) containing much of the human CB1 receptor C-terminal tail for use as standard and negative control, respectively, in quantitative Western blot analysis of CB1 receptor expression on crude synaptosomes of the adult rat brain cortex. To this end we used three different antibodies, all raised against a peptide comprising the C-terminal residues 443-473 of the mouse CB1 receptor that corresponds to residues 442-472 in the human homolog. Estimated values of CB1 receptor density obtained by quantitative Western blot were of the same order of magnitude but slightly higher than values obtained by the radioligand saturation binding assay. Conclusions: Collectively, here we provide a suitable Western blot-based design as a simple, cost-effective and radioactivity-free alternative for the quantitative analysis of CB1 receptor expression, and potentially of any GPCR, in a variety of biological samples. The discrepancies between the results obtained by quantitative Western blot and radioligand saturation binding techniques are discussed in the context of their particular theoretical bases and methodological constraints.This work was funded by Spanish Ministry of Science, Innovation and Universities (Grant ID, CTQ2017-85686-R) and Basque Government (Research Groups of the Basque University System, Grant IDs, IT1492-22 and IT1620-22)

An Overview of Ocean Climate Change Indicators: Sea Surface Temperature, Ocean Heat Content, Ocean pH, Dissolved Oxygen Concentration, Arctic Sea Ice Extent, Thickness and Volume, Sea Level and Strength of the AMOC (Atlantic Meridional Overturning Circulation)

Global ocean physical and chemical trends are reviewed and updated using seven key ocean climate change indicators: (i) Sea Surface Temperature, (ii) Ocean Heat Content, (iii) Ocean pH, (iv) Dissolved Oxygen concentration (v) Arctic Sea Ice extent, thickness, and volume (vi) Sea Level and (vii) the strength of the Atlantic Meridional Overturning Circulation (AMOC). The globally averaged ocean surface temperature shows a mean warming trend of 0.062 ± 0.013 ºC per decade over the last 120 years (1900–2019). During the last decade (2010–2019) the rate of ocean surface warming has accelerated to 0.280 ± 0.068 ºC per decade, 4.5 times higher than the long term mean. Ocean Heat Content in the upper 2,000 m shows a linear warming rate of 0.35 ± 0.08 Wm-2 in the period 1955–2019 (65 years). The warming rate during the last decade (2010–2019) is twice (0.70 ± 0.07 Wm-2) the warming rate of the long term record. Each of the last six decades have been warmer than the previous one. Global surface ocean pH has declined on average by approximately 0.1 pH units (from 8.2 to 8.1) since the industrial revolution (1770). By the end of this century (2100) ocean pH is projected to decline additionally by 0.1-0.4 pH units depending on the RCP (Representative Concentration Pathway) and SSP (Shared Socioeconomic Pathways) future scenario. The time of emergence of the pH climate change signal varies from 8 to 15 years for open ocean sites, and 16-41 years for coastal sites. Global dissolved oxygen levels have decreased by 4.8 petamoles or 2% in the last 5 decades, with profound impacts on local and basin scale habitats. Regional trends are varying due to multiple processes impacting dissolved oxygen: solubility change, respiration changes, ocean circulation changes and multidecadal variability. Arctic sea ice extent has been declining by -13.1% per decade in summer (September) and by -2.6% per decade in winter (March) during the last 4 decades (1979–2020). The combined trends of sea ice extent and sea ice thickness indicate that the volume of non-seasonal Arctic Sea Ice has decreased by 75% since 1979. Global mean sea level has increased in the period 1993–2019 (the altimetry era) at a mean rate of 3.15 0.3 mm year-1 and is experiencing an acceleration of ~ 0.084 (0.06–0.10) mm year-2. During the last century (1900–2015; 115y) global mean sea level (GMSL) has rised 19 cm, and near 40% of that GMSL rise has taken place since 1993 (22y). Independent proxies of the evolution of the Atlantic Meridional Overturning Circulation (AMOC) indicate that AMOC is at its weakest for several hundreds of years and has been slowing down during the last century. A final visual summary of key ocean climate change indicators during the recent decades is provided.Versión del edito