Drought tolerance conferred to sugarcane by association with Gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways

Sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass. Other benefits, as enhanced tolerance to abiotic stresses have been reported to some diazotrophs. Here we aim to study the effects of the association between the diazotroph Gluconacetobacter diazotrophicus PAL5 and sugarcane cv. SP70-1143 during water depletion by characterizing differential transcriptome profiles of sugarcane. RNA-seq libraries were generated from roots and shoots of sugarcane plants free of endophytes that were inoculated with G. diazotrophicus and subjected to water depletion for 3 days. A sugarcane reference transcriptome was constructed and used for the identification of differentially expressed transcripts. The differential profile of non-inoculated SP70-1143 suggests that it responds to water deficit stress by the activation of drought-responsive markers and hormone pathways, as ABA and Ethylene. qRT-PCR revealed that root samples had higher levels of G. diazotrophicus 3 days after water deficit, compared to roots of inoculated plants watered normally. With prolonged drought only inoculated plants survived, indicating that SP70-1143 plants colonized with G. diazotrophicus become more tolerant to drought stress than non-inoculated plants. Strengthening this hypothesis, several gene expression responses to drought were inactivated or regulated in an opposite manner, especially in roots, when plants were colonized by the bacteria. The data suggests that colonized roots would not be suffering from stress in the same way as non-inoculated plants. On the other hand, shoots specifically activate ABA-dependent signaling genes, which could act as key elements in the drought resistance conferred by G. diazotrophicus to SP70-1143. This work reports for the first time the involvement of G. diazotrophicus in the promotion of drought-tolerance to sugarcane cv. SP70-1143, and it describes the initial molecular events that may trigger the increased drought tolerance in the host plant

Exacerbated inflammatory cellular immune response characteristics of HAM/TSP is observed in a large proportion of HTLV-I asymptomatic carriers

BACKGROUND: A small fraction of Human T cell Leukemia Virus type-1 (HTLV-I) infected subjects develop a severe form of myelopathy. It has been established that patients with HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP) show an exaggerated immune response when compared with the immunological response observed in HTLV-I asymptomatic carriers. In this study the immunological responses in HAM/TSP patients and in HTLV-I asymptomatic carriers were compared using several immunological assays to identify immunological markers associated with progression from infection to disease. METHODS: Immunoproliferation assays, cytokine levels of unstimulated cultures, and flow cytometry analysis were used to evaluate the studied groups. Nonparametric tests (Mann-Whitney U test and Wilcoxon matched-pairs signed ranks) were used to compare the difference between the groups. RESULTS: Although both groups showed great variability, HAM/TSP patients had higher spontaneous lymphoproliferation as well as higher IFN-γ levels in unstimulated supernatants when compared with asymptomatic carriers. Flow cytometry studies demonstrated a high frequency of inflammatory cytokine (IFN-γ and TNF-α) producing lymphocytes in HAM/TSP as compared to the asymptomatic group. This difference was accounted for mainly by an increase in CD8 cell production of these cytokines. Moreover, the HAM/TSP patients also expressed an increased frequency of CD28-/CD8+ T cells. Since forty percent of the asymptomatic carriers had spontaneous lymphoproliferation and IFN-γ production similar to HAM/TSP patients, IFN-γ levels were measured eight months after the first evaluation in some of these patients to observe if this was a transient or a persistent situation. No significant difference was observed between the means of IFN-γ levels in the first and second evaluation. CONCLUSIONS: The finding that a large proportion of HTLV-I carriers present similar immunological responses to those observed in HAM/TSP, strongly argues for further studies to evaluate these parameters as markers of HAM/TSP progression

Spatial dependence of attributes of rainfed maize under distinct soil cover conditions

ABSTRACT Maize crop under rainfed conditions is widely grown in the Brazilian Northeast on community farms for human and animal consumption. The application of mulch could be adopted to enhance rainwater harvesting and soil moisture levels, although such practice is rarely applied in rainfed cultivation. This study aimed to evaluate the spatial variability of maize productivity, based on soil physical attributes and plant growth and yield, under a rainfed regime in the presence and absence of mulch in the Brazilian semi-arid region. The study was conducted from May to September 2016 in Pesqueira - PE (locality of the Semiarid of Pernambuco State, Northeast, Brazil), during which the development and production of maize in experimental plots were evaluated, and maize was grown using the traditional cropping system with and without mulching. Two grids with 40 points in a 4 × 10 m mesh were established, with spacing of 1 m for soil sampling and maize biometric evaluation. For statistical analysis, 30 samples were randomly selected from each plot. Maize cultivated in the presence of mulch showed higher development, with symmetrical distribution for all variables except for soil moisture and stem diameter. Sand content, soil moisture, height of the first cob, number of leaves, leaf length, plant height, and cob weight presented moderate to strong spatial scale dependence for the two cropping conditions, with higher spatial dependence consistently observed for the development parameters in the covered area

Spatial variability of biophysical multispectral indexes under heterogeneity and anisotropy for precision monitoring

ABSTRACT The study aimed to characterize the spatial structure of variability of biophysical indexes of vegetation through images obtained by Unmanned Aerial Vehicles under strong heterogeneity and anisotropy, using geostatistical procedures. Plots with different types and densities of culture were evaluated in a didactic vegetable garden. Five vegetation indexes obtained from aerial multispectral camera images were evaluated parallel with geostatistical analysis and anisotropy investigation for multiscale spatial modeling. For the studied domain, geometric anisotropy was identified for the biometric indexes. The spherical model presented a better fit when anisotropy was not considered, whereas the exponential model had the best performance in the anisotropic analysis. Contrasting targets were better identified in multispectral images and considering anisotropy. The Soil-Adjusted Vegetation Index is recommended for similar applications

Coupling Water Resources and Agricultural Practices for Sorghum in a Semiarid Environment

Water scarcity and changing rainfall distribution have caused uncertainties in relation to agricultural production in semiarid areas. In this context, water reuse for irrigation is a promising alternative, although requiring irrigation and agricultural management. Production of forage plants is strategic for semiarid areas due to their high tolerance to stresses and use as animal fodder. The objective of this work was to evaluate the combined performance of treated wastewater irrigation and mulching on forage sorghum and on soil attributes in Northeast Brazil. Sorghum was cropped in November 2018, three months before the beginning of the hydrologic year, and cultivated over three cycles until April 2019. The experiment was designed with five irrigation depths (60%, 80%, 100%, 120%, and 140% of crop evapotranspiration), and with four soil cover conditions (0% (witness), soil covered with native vegetation, with coconut coir, and with macerated moringa seeds), adopting four replications. Irrigation with treated wastewater promoted linear increases in forage sorghum yield at irrigation depths of up to 140% of crop evapotranspiration. Mulch promoted a 24% increase in productivity in relation to the area where conservation was not practiced, being able to control salinity while also contributing to the higher incorporation of organic matter. Irrigation with treated wastewater had no negative agronomic impacts on soil, once natural rainfall events typical of the Brazilian semiarid region allowed effective salt leaching from shallow sandy soils. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The National Council for Scientific and Technological Development—CNPq (151969/2020-5); the Brazilian Funding Authority for Studies and Projects—FINEP; the Foundation of Science and Technology Support for Pernambuco State—FACEPE (IBPG-1758-5.03/15 Scholarship and “Tecnologias Hídricas para o semiárido” Project—Grant APQ 0300-5.03/17); the Coordination for the Improvement of Higher Education Personnel (CAPES, Finance code 001; the CAPES/PrInt–UFRPE Programme for internationalization) to the Agronomic Institute of Pernambuco—IPA; and also the Federal Rural University Postgraduate Programme in Agricultural Engineering

Soil moisture in forage cactus plantations with improvement practices for their resilience

ABSTRACT The present study evaluated soil moisture (θ) in forage cactus plantations under resilience practices such as irrigation, mulching and intercropping with sorghum. The experiment was arranged in a randomized block design with five water availability conditions (0, 8.75, 17.5, 26.25 and 35% of the reference evapotranspiration), and three planting systems: forage cactus exclusive system (PE), forage cactus with mulch (PC) and intercropping between forage cactus and sorghum (PS). Water content was measured until a depth of 0.60 m. Meteorological data were recorded between June 2012 and June 2013. Water content was not affected by different resilience practices and the variation coefficient was low to medium (4.0 to 22.1%). The temporal variation of soil moisture was more affected by the changes in rainfall, while the physical properties (soil density, porosity and grain size) were decisive in the vertical variation. It was concluded that periods with rainfall events favor the alternation of practices to improve forage cactus resilience (irrigation, mulch and intercropping)

Educação popular em Saúde Mental: relato de uma experiência Popular education in Mental Health: experience report

Este artigo tem o objetivo de relatar uma experiência de educação popular em Saúde Mental, no contexto do movimento de Reforma Psiquiátrica, realizada no bairro de Pernambués, território do Centro de Atenção Psicossocial Álcool e Outras Drogas, localizado no município de Salvador-BA e campo de prática dos residentes do Núcleo de Saúde Mental da Residência Multiprofissional em Saúde da Universidade do Estado da Bahia - UNEB. Tal estratégia foi viabilizada através da realização de fóruns comunitários envolvendo vários atores sociais. Adotou-se neste trabalho a metodologia da sistematização da experiência proposta por Holliday, utilizando-se, para tanto, de registros de observações em diários de campo. Ao dar voz e vez a cada participante/falante, numa relação de valorização de saber e poder, a estratégia da educação popular permitiu que os sujeitos envolvidos no processo se organizassem politicamente e participassem da construção coletiva de novos saberes e práticas no campo da Saúde Mental, condizentes com a valorização da vida em sua multiplicidade.<br>This article presents an experience of popular education in mental health in the context of the Psychiatric Reform. Such experience occurred in the district of Pernambués, territory of the Centro de Atenção Psicossocial Álcool e Outras Drogas (Center for Psychosocial Assistance Alcohol and Other Drugs), located in the city of Salvador, state of Bahia. This center is the field for the practice of interns of the Mental Health Nucleus of the Multiprofessional Health Internship of Universidade do Estado da Bahia (UNEB - University of the State of Bahia). Such strategy was enabled by the organization of community forums involving many social actors. In this work, we used the methodology of experience systematization proposed by Holliday. By giving voice and time to each speaker/participant, in equal levels of knowledge and power, the popular education strategy allowed the people involved in this process to politically organize themselves. In addition, these people participated in the collective construction of new knowledge and practices in the mental health area, in keeping with the valuation of life in its multiplicity