The clinical value of avidity test in the management of serologic rubella infection: A case report

Yirmi sekiz yaşında gebe hastanın ilk başvurusunda ve gebeliğin 11, 13, 30 ve 36. haftalarında rubella IgM ve IgG testleri pozitif çıktı. Rubella avidite testinin yüksek titreli değer göstermesi üzerine hastanın gebeliği takibe alınarak sağlıklı bir bebek doğurması sağlandı. Rubella enfeksiyonu asemptomatik olabilir ve re-enfeksiyon da subklinik seyir gösterebilir. Rubella antikor titrelerinin tüm gebelik boyunca seyri dikkate alındığında tanıya varmak kolaydır. Kritik nokta hastanın ilk gelişindeki sonuçlarına göre hastalığın durumunu ayırt ederek karar verebilmektir. Avidite testi, bu tip sonuçlar alındığında hasta yönetiminde bize yol gösterecektir.A 28-year-old pregnant woman presented with positive rubella IgM and IgG antibodies at initial admission and at the 11th, 13th, 30th and 36th weeks of gestation. Rubella avidity test revealed high titers and the patient was followed-up to ensure birth of a healthy infant. Rubella infection may be asymptomatic and re-infection may demonstrate a subclinical course. It is easy to make the diagnosis of rubella by following the antibody titers during pregnancy. The critical point in such patients is to differentiate the condition of disease at admission and decide accordingly. Avidity test may be a useful method for the management of such patients

Interview with Marjorie Harkins Buchanan Kiewit

In this interview with Julia Stringfellow, Marjorie Harkins Buchanan Kiewit, LU class of 1943, discusses her time as a student as well as her time on the Board of Trustees.https://lux.lawrence.edu/oralhistories/1036/thumbnail.jp

The interrelations of radiologic findings and mechanical ventilation in community acquired pneumonia patients admitted to the intensive care unit: a multicentre retrospective study

BACKGROUND: We evaluated patients admitted to the intensive care units with the diagnosis of community acquired pneumonia (CAP) regarding initial radiographic findings. METHODS: A multicenter retrospective study was held. Chest x ray (CXR) and computerized tomography (CT) findings and also their associations with the need of ventilator support were evaluated. RESULTS: A total of 388 patients were enrolled. Consolidation was the main finding on CXR (89%) and CT (80%) examinations. Of all, 45% had multi-lobar involvement. Bilateral involvement was found in 40% and 44% on CXR and CT respectively. Abscesses and cavitations were rarely found. The highest correlation between CT and CXR findings was observed for interstitial involvement. More than 80% of patients needed ventilator support. Noninvasive mechanical ventilation (NIV) requirement was seen to be more common in those with multi-lobar involvement on CXR as 2.4-fold and consolidation on CT as 47-fold compared with those who do not have these findings. Invasive mechanical ventilation (IMV) need increased 8-fold in patients with multi-lobar involvement on CT. CONCLUSION: CXR and CT findings correlate up to a limit in terms of interstitial involvement but not in high percentages in other findings. CAP patients who are admitted to the ICU are severe cases frequently requiring ventilator support. Initial CT and CXR findings may indicate the need for ventilator support, but the assumed ongoing real practice is important and the value of radiologic evaluation beyond clinical findings to predict the mechanical ventilation need is subject for further evaluation with large patient series

Posttraumatic tibial osteomyelitis caused by Pseudomonas putida

WOS: 000255444000009Pseudomonas putida is a Gram-negative, nonfermentative, saprophytic soil bacterium with versatile metabolical features. We presented an 18-year-old male patient who developed tibial osteomyelitis due to P. putida after a traffic accident. He had open comminuted fractures in the right fibula and tibia and the site of open fracture was contaminated with dust and soil. He underwent surgical debridement and irrigation of the fracture site followed by fracture reduction and application of an external fixator. Despite empirical antibiotic treatment, there was a discharge from the pin tract on the postoperative fifth day. Cultures obtained from soft tissue and bone during repeat debridement and irrigation yielded P. putida. The patient recovered after four weeks of parenteral antibiotic treatment without any sequela. A case of posttraumatic tibial osteomyelitis caused by P. putida has hitherto not been reported in the literature

Posttraumatic tibial osteomyelitis caused by Pseudomonas putida

Pseudomonas putida, toprakta yaşayan, metabolik özellikleri değişken olan, Gram-negatif nonfermentatif bir basildir. Bu yazıda trafik kazası sonrasında P. putida’nın etken olduğu tibial osteomiyelit gelişen 18 yaşında bir erkek hasta sunuldu. Hastada sağ fibula ve tibiada açık parçalı kırık vardı ve kırık alanı toz, toprak gibi maddelerle kontamine olmuştu. Tedavide, açık kırık alanına cerrahi debridman uygulandı, irigasyonla yara alanındaki yabancı maddeler temizlendi. Sağ tibiaya kırık redüksiyonu yapıldı ve eksternal fiksatör uygulandı. Ampirik antibiyotik tedavisine rağmen ameliyat sonrası beşinci günde hastanın çivi dibinden akıntısı vardı. Tekrarlanan debridman ve irigasyon sırasında yumuşak dokudan ve kemikten alınan kültür örneklerinde P. putida üredi. Hasta dört hafta süreyle uygulanan parenteral antibiyotik tedavisi sonrasında sekel kalmaksızın iyileşti. Literatürde bu mikroorganizmanın etken olduğu osteomiyelit bildirimine rastlanmamıştır.Pseudomonas putida is a Gram-negative, nonfermentative, saprophytic soil bacterium with versatile metabolical features. We presented an 18-year-old male patient who developed tibial osteomyelitis due to P. putida after a traffic accident. He had open comminuted fractures in the right fibula and tibia and the site of open fracture was contaminated with dust and soil. He underwent surgical debridement and irrigation of the fracture site followed by fracture reduction and application of an external fixator. Despite empirical antibiotic treatment, there was a discharge from the pin tract on the postoperative fifth day. Cultures obtained from soft tissue and bone during repeat debridement and irrigation yielded P. putida. The patient recovered after four weeks of parenteral antibiotic treatment without any sequela. A case of posttraumatic tibial osteomyelitis caused by P. putida has hitherto not been reported in the literature

Momordica charantia Inhibits Prostate Cancer Cell Proliferation by Suppressing Dihydrotestosterone-Induced Caspase-9 Phosphorylation

Momordica charantia is gaining popularity as a complementary medicine due to its high nutritional content and possibly anti-cancer properties. Here, we show how Momordica charantia seed extract (McSE) enhances growth inhibition and migration of LNCaP cells and the possible mechanisms of action responsible for these effects. McSE has been found to block both cell growth with an IC50 value of 36,3 μg/mL and dihydrotestosterone (DHT)-induced cell migration. The non-genomic signaling kinases ERK1/2 and AKT were activated by short-term (5 and 15-min) low concentrations (1 nM) of DHT, followed by the phosphorylation of caspase-9. Due to an increase in caspase-9 phosphorylation in cells, DHT caused a reduction in the expression of cleaved caspase-3. Treatment with McSE significantly reduced the activation of ERK1/2 and AKT and the phosphorylation of caspase-9. Further, the levels of cleaved caspase-3 increased because of treatment with McSE, and the morphology of the cells underwent typical apoptotic changes. The results show that McSE may be a source of newly discovered anti-cancer molecules for potential cancer drug development

A Case of Pulmonary Leptospirosis Treated with a Combination of Corticosteroid and Ceftriaxone

In leptospirosis, pulmonary involvement is the main cause of death. The survival in pulmonary leptospirosis depends on early and appropriate therapy as well as rapid diagnosis. Some immunosuppressive drugs have been known to improve survival because infectious vasculitis is responsible for the pathogenesis of pulmonary damage caused by leptospirosis. In this study, we aimed to report a case of pulmonary leptospirosis not frequently reported in our country or may be overlooked in clinical practice that was successfully treated with corticosteroids in addition to ceftriaxone therapy. A 32-year-old man presented to our hospital with fever, malaise, dry cough, shortness of breath, hemoptysis, and general muscle ache persisting for one week. He had a history of cleaning a barn with mice barefoot two weeks ago. The patient had fever, thrombocytopenia, elevated creatinine, bilirubin, and creatine kinase levels, bilateral pulmonary infi ltrates in chest radiograph, decreased peripheral saturation of hemoglobin with oxygen and epidemiological history compatible with leptospirosis. Due to high index of suspicion of pulmonary leptospirosis, high dose prednisolone was administered at the dose of 1 gr/day for the fi rst 3 days, followed by oral prednisolone 1 mg/kg/day for 7 days alongside ceftriaxone. Rapid clinical improvement was observed. The patient did not need mechanical ventilation and was discharged without sequelae. Consequently, pulmonary leptospirosis should be considered in differential diagnosis of patients with febrile illness associated with pneumonitis and respiratory failure, and it should not be forgotten that corticosteroids therapy may be lifesaving

The removal of Cr(VI) through polymeric supported liquid membrane by using calix[4]arene as a carrier

In this work, the transport and removal of Cr(VI) were achieved through supported liquid membrane (SLM) by using a 5,17, di-tert-butyl-11,23-bis[(1,4-dioxa-8-azaspiro [4,5]decanyl)methyl]-25,26,27,28-tetrahydroxy calix[4]arene carrier, dissolved in 2-nitrophenyl octyl ether dichloromethane. The studied parameters are the solvent effect in the membrane phase, the effect of carrier concentration, and the acid type in the donor phase. The Celgard 2500 was used as a membrane support. We used the Danesi mass transfer model to calculate the permeability coefficients for each studied parameter. In addition, AFM and SEM techniques were used to characterize the surface morphology of the prepared Celgard 2500 membrane that included the calix[4]arene carrier. © 2018 Elsevier B.V

Comparison of natural NQO2 inhibitors as a new target for cancer treatment in different cell lines

Objective: Quinones are highly reactive compounds undergoing either one-or two-electron reductions. Enzymatic one-electron reduction generates unstable semiquinone radicals which readily undergo redox cycling over NQO1 and NQO2 enzymes, in the presence of molecular oxygen, leading to the production of highly reactive oxygen species (ROS). NQO1 is responsible form detoxification where NQO2 inhibitors are found to show potent anticancerogenic effects. The aim of this study is to investigate some natural inhibitors of the NQO2 enzyme and to identify their action mechanisms over this enzyme on different cancer cell lines