9 research outputs found
Mantle Cell Hyperplasia of Peripheral Lymph Nodes as Initial Manifestation of Sickle Cell Disease
Sickle cell disease (SCD) is a well known hemoglobinopathy with usual manifestations including anemia, hyperbilirubinemia, and
vasoocclusive complications. Despite presence of mild splenomegaly in early phase of the disease, lymphadenopathy is not an
often finding of SCD. We introduce an undiagnosed case of SCD who presented in third decade of his life with multiple cervical
lymphadenopathies and mild splenomegaly persistent for about five years. Histopathologic examination of the resected lymph
nodes showed expansion of the mantle cell layers of secondary follicles as well as several monomorphic mantle cell nodules. To
rule out possibility of a malignant process involving lymph nodes, an immunohistochemical panel was ordered which was in favor
of benign mantle cell hyperplasia. Immunoglobulin gene rearrangement study showed no clonal bands and confirmed benign
nature of the process. Respecting mild abnormalities on Complete Blood Count, peripheral blood smear was reviewed revealing
some typical sickle red blood cells as well as rare nucleated red blood cells. Solubility test for hemoglobin (HB) S was positive.
Hemoglobin electrophoresis confirmed diagnosis of homozygous HbS disease
Mantle Cell Hyperplasia of Peripheral Lymph Nodes as Initial Manifestation of Sickle Cell Disease
Sickle cell disease (SCD) is a well known hemoglobinopathy with usual manifestations including anemia, hyperbilirubinemia, and
vasoocclusive complications. Despite presence of mild splenomegaly in early phase of the disease, lymphadenopathy is not an
often finding of SCD. We introduce an undiagnosed case of SCD who presented in third decade of his life with multiple cervical
lymphadenopathies and mild splenomegaly persistent for about five years. Histopathologic examination of the resected lymph
nodes showed expansion of the mantle cell layers of secondary follicles as well as several monomorphic mantle cell nodules. To
rule out possibility of a malignant process involving lymph nodes, an immunohistochemical panel was ordered which was in favor
of benign mantle cell hyperplasia. Immunoglobulin gene rearrangement study showed no clonal bands and confirmed benign
nature of the process. Respecting mild abnormalities on Complete Blood Count, peripheral blood smear was reviewed revealing
some typical sickle red blood cells as well as rare nucleated red blood cells. Solubility test for hemoglobin (HB) S was positive.
Hemoglobin electrophoresis confirmed diagnosis of homozygous HbS disease
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Cutaneous variant of angiokeratoma corporis diffusum associated with angiokeratoma circumscriptum
Unusual Presentation of Squamous Cell Carcinoma on Long-Standing Sacrococcygeal Pilonidal Sinus
Pilonidal disease consists of a hair-containing sinus or abscessoccurring most frequently in intergluteal cleft. This disease isgenerally benign. Although very uncommon entity, it seemsreasonable to be aware of possible malignancy in longstandingcases. We report a case of squamous cell carcinoma in a 52-year-old man, with a prolonged history of pilonidal diseaseand ulceration since 3 months before referring to our clinic.We excised the cyst, and the pathologic evaluation reportedmoderate differentiated squamous cell carcinoma. So, reoperationon the lesion site to excise a 2-cm margin wasperformed and the defect was covered with Limberg cutaneusflap. We recommend early excision of pilonidal cysts toprevent possible malignant degeneration. Histologicalexamination of the excised materials to prevent missing raremalignant cases is recommended
The effect of zinc on healing of renal damage in rats
Background: Several studies have previously been performed to promote kidney healing after injuries. Objectives: The aim of this study was to investigate the effect of zinc on renal healing after traumatic injury in rats. Materials and Methods: Forty healthy female rats were selected and one of their kidneys was incised. Half of the incisions were limited only to the cortex (renal injury type I) and the other ones reached the pelvocalyceal system of the kidney (renal injury type II). All the rats in the zinc treated group (case group) received 36.3 mg zinc sulfate (contained 8.25 mg zinc) orally. After 28 days, the damaged kidneys were removed for histopathological studies. Results: In the rats with type I injury, kidney inflammation of the case group was significantly lower than that of the control group. However, the result was not significant in rats with type II injury. Tissue loss and granulation tissue formation were significantly lower in the case group than the control group in both type I and II kidney injuries. Conclusions: Overall, Zinc can contribute to better healing of the rat’s kidneys after a traumatic injury
Effect of combined density gradient centrifugation on X- and Y- sperm separation and chromatin integrity
Background: It has been claimed that by using different washing
methods, the sperms can be separated according to size, motility,
density, chromosomal content and surface markings and charge. These
methods also reduce sperm chromatin deficiencies and screen the sperms
before applying in assisted reproduction techniques. Objective: This
study compared simple density gradient methods and a combined method
with albumin density gradient and PureSperm separation (alb/PureSperm)
for sex preselection by double fluorescence in situ hybridization
(FISH) versus chromomycin A3 staining to determine chromatin integrity.
Materials and Methods: 30 normal semen samples were prepared with
PureSperm, albumin gradients and alb/PureSperm. All samples were then
stained by FISH and chromomycin A3. The results were compared with SPSS
11.5 and the Kruskal-Wallis test. Results: The proportion of X-bearing
spermatozoa by PureSperm separation (47.58±5.67) and Y-bearing
spermatozoa by albumin gradient (46.13±3.83) methods were slightly
higher than in putative normal sperm samples (1:1), but there were no
significant differences in the X- or Y- bearing spermatozoa counts
among the three methods. Albumin gradient separation tended to
underestimate abnormal spermatozoa compared to PureSperm and combined
alb/PureSperm. Conclusion: Routine separation methods slightly enriched
X- or Y- bearing spermatozoa, but the differences were not significant
for clinical purposes. The combined alb/PureSperm method had no
advantages for assessing sex ratio or chromatin integrity compared to
simpler gradient methods