Structure and functional significance of branched anastomosing mucosal folds in the proximal intestine of the farmed African catfish (Clarias gariepinus Burchell 1822)

Histology has provided morphologist a powerful tool that enhances the nature, form and detailed structure of organs. The proximal intestine though a more muscular tube than the rest of the intestine presented a complex anastomosing mucosal fold resembling a honey comb-like structure. This specialization was not seen in the other intestinal segments. This specialization increases the surface area for nutrient absorption. This probably compensates for shortness of intestine and absence of pyloric caeca in this species. The glycoconjugates in the goblet cell of proximal intestine mucosal fold were subjected to mucin  histochemical study. The study revealed the presence of neutral andacid mucin but acid mucin was predominant. The neutral mucin can be associated to buffering the effect of gastric acid, transport of small disaccharide molecules. The predominating acid mucin suggest air-breathing function since the acid will always make the epithelium moist for gaseous exchange between dissolved air in   swallowed water and abundant blood vessels present in the tunica mucosa.Keywords: Nutrient absorption, Proximal intestine, Anastomosing mucosal folds, Histology, Histochemistry, Clarias gariepinu

Distal Intestinal Caecum of Farmed African Catfish (Clarias gariepinus Burchell, 1822): A Case Report

The microanatomical characteristics of the distal intestinal caecum were investigated. The histology was typical of tubular organs containing tunicamucosa, submucosa, muscularis and adventitia. The lumen was lined by simple mucosal folds. The covering epithelium was of simple columnar cells containing goblet cells and intraepithelial lymphocytes. The lamina propria contained blood vessels, loose connective tissue and leukocytes.The muscularis mucosae of smooth muscle cells were observed. The submucosa contained loose connective tissue, smooth muscle cells, bloodvessels and leukocytes. The tunica muscularis contained a myenteric plexus between the inner circular and outer longitudinal layers of smoothmuscle cells. The mucin histochemistry revealed that the goblet cells present contained neutral and acid mucin but acid mucin predominated suggesting an organ with more need for protection against bacteria in the blind ended tube. When compared with the histology of the intestinal segments cranial and caudal to the diverticulum, the features of thecaecum were very similar to the segment cranial to it necessitating the conclusion that the diverticulum is the distal intestinal caecum. The histology suggests an organ serving as complementary digestivestructure to distal intestine in nutrient absorption without increasing the space occupied by the digestive tract.KEYWORDS: DISTAL, INTESTINAL, CAECUM, FARMED, AFRICAN, CATFISH, CASE REPOR

The effects of singeing on the histological appearance of some visceral organs in the red Sokoto goat (Capra hirrcus)

The histology of visceral organs from singed Red Sokoto goats were assessed for reliability for use as histopathological samples from singed abattoir specimen. This becomes important as samples for histopathology and histology of goats in Nigeria have been sourced from singed carcasses, with no available literature reporting on the effect of singeing on the histological appearance of tissues from such sample. Hence, this study will fill this knowledge gap. The unfixed samples post-singeing were grouped into time periods of an hour interval up to 3 hours. They were subsequently fixed after allocated tie framed and processed for histological observation. The slides were graded on a scale of 1 to 3, where 1 referred to highly distorted, 2 referred to moderately distorted and 3 referred to mildly distorted. The samples from unsigned viscera that were fixed immediately served as control. Most of the visceral organs showed moderate distortions, except the intestines which degenerated to highly distorted architecture after 3 hours. The stomach compartments displayed normal organ structure similar to the control samples. Blood extravasations were observed in most of the organs. This project shows that samples from singed carcasses that are fixed within 2 hours post-singeing are stable for histology or histopathological studies. It also implied that abattoir samples for histopathogy should be sent to the nearest laboratory about 2 hours radius for fixation and processing to generate reliable data.Key words: Singeing, organ architecture, fixation, histology, histopathology, Nigeri

Morfología de la cavidad orofaríngea y esófago del bagre africano adulto (<i>Clarias gariepinus</i> Burchell, 1822)

The morphology of the adult farmed African catfish -Clarias gariepinus a prominent omnivorous cultivable species in Nigeria was investigated. It revealed cornified horny plates lining the buccal surfaces of the lips. The tongue was not free moving, and the oesophagus was a narrow tube connecting the oropharyngeal cavity to the stomach with no valve or constriction separating it from the later. Histologically, the cavity wall was lined by stratified squamous epithelium containing eosinophilic club cells, mucous cells and taste buds. The laminar propria contained dense collagen fibres. No taste bud was seen on the tongue suggesting it non involvement in food selection through gustation unlike the cavity wall which contained taste buds. The oesophagus was lined by stratified mucous epithelium containing club cells. Histochemistry revealed the presence of neutral, acid and combination of the two. The large extent of oesophageal mucification indicates need for mucin lubrication of food since teleost is known to lack salivary glands and also an adaptation to ingestion of varied feed.La morfología bagre africano del adulto cultivado -Clarias gariepinus- una especie omnívoras cultivables prominente en Nigeria fue investigada. Reveló placas córneas que cubren las superficies vestibulares de los labios. La lengua no tiene movimiento libre y el esófago es un tubo estrecho que conecta la cavidad orofaríngea al estómago sin válvula o constricción que lo separe. Histológicamente, la pared de la cavidad estaba revestida por epitelio escamoso estratificado que contiene células eosinófilas, células mucosas y papilas gustativas. La laminar propia contenía fibras de colágeno denso. No fie vista ninguna papila gustativa en la lengua lo que sugiere la no participación la selección de los alimentos a través gustación a diferencia de la pared de la cavidad que contiene papilas gustativas. El esófago estaba revestido por epitelio estratificado que contiene células mucosas. La histoquímica reveló la presencia de ácido a neutro, y la combinación de los dos. La gran extensión de mucificación esofágica indica la necesidad de lubricación de mucina de los alimentos se sabe que los teleósteos carecen de glándulas salivales y también una adaptación a la ingestión de alimentos variados.Facultad de Ciencias Veterinaria

Morfología de la cavidad orofaríngea y esófago del bagre africano adulto (<i>Clarias gariepinus</i> Burchell, 1822)

The morphology of the adult farmed African catfish -Clarias gariepinus a prominent omnivorous cultivable species in Nigeria was investigated. It revealed cornified horny plates lining the buccal surfaces of the lips. The tongue was not free moving, and the oesophagus was a narrow tube connecting the oropharyngeal cavity to the stomach with no valve or constriction separating it from the later. Histologically, the cavity wall was lined by stratified squamous epithelium containing eosinophilic club cells, mucous cells and taste buds. The laminar propria contained dense collagen fibres. No taste bud was seen on the tongue suggesting it non involvement in food selection through gustation unlike the cavity wall which contained taste buds. The oesophagus was lined by stratified mucous epithelium containing club cells. Histochemistry revealed the presence of neutral, acid and combination of the two. The large extent of oesophageal mucification indicates need for mucin lubrication of food since teleost is known to lack salivary glands and also an adaptation to ingestion of varied feed.La morfología bagre africano del adulto cultivado -Clarias gariepinus- una especie omnívoras cultivables prominente en Nigeria fue investigada. Reveló placas córneas que cubren las superficies vestibulares de los labios. La lengua no tiene movimiento libre y el esófago es un tubo estrecho que conecta la cavidad orofaríngea al estómago sin válvula o constricción que lo separe. Histológicamente, la pared de la cavidad estaba revestida por epitelio escamoso estratificado que contiene células eosinófilas, células mucosas y papilas gustativas. La laminar propia contenía fibras de colágeno denso. No fie vista ninguna papila gustativa en la lengua lo que sugiere la no participación la selección de los alimentos a través gustación a diferencia de la pared de la cavidad que contiene papilas gustativas. El esófago estaba revestido por epitelio estratificado que contiene células mucosas. La histoquímica reveló la presencia de ácido a neutro, y la combinación de los dos. La gran extensión de mucificación esofágica indica la necesidad de lubricación de mucina de los alimentos se sabe que los teleósteos carecen de glándulas salivales y también una adaptación a la ingestión de alimentos variados.Facultad de Ciencias Veterinaria

Surgical Abdomen in School Age Children: A Prospective Review From Two Centers In SouthWestern Nigeria

Background: Surgical abdomen traverses all age groups. We sought to define the aetiology, patients’ characteristics, and outcome of management amongst children Methods: Two years prospective review of patients aged 5-15 years managed for surgical abdomen at the Wesley Guilds Hospital Ilesa and Mishmael Medical Centre Akure, Nigeria. Results: Fifty two patients were treated. The male: female ratio was 1:1. The age range was 5years to 15years (mean=11.25 ±2.24years). Mean duration of illness was 29.5hours (range 2-72hours). Gut perforation was the most common aetiology (n=39; 75%); with perforations due to infections most prevalent (n= 34; 87.2%). Ten cases (19.2%) were trauma related and showed male predominance. Obstructed gut accounted for 15.4% (n=8) of cases; and showed female predominance. Five out of the eight small bowel obstructions (62.5%) were due to post operation adhesions. Pre-operative and post-operative diagnoses were congruent in 90.4% (n=47) of cases. Major post-operative complications were surgical site infection (20; 38.5%), and pneumonia (5; 9.6%). The average hospital stay was 9days (range 4-21days). Mortality rate was 1.9% (n=1). Conclusion: Acute abdomen requiring surgical intervention is mainly infective origin. The male child is more at risk of abdominal trauma while gut obstruction was more common in females.Keywords: Surgical, Abdomen, Children, Emergenc

Morphology of the oropharyngeak cavity and oesophagus of the farmed adult African catfish (Clarias gariepinus Burchell, 1822)

The morphology of the adult farmed African catfish -Clarias gariepinus a prominent omnivorous cultivable species in Nigeria was investigated. It revealed cornified horny plates lining the buccal surfaces of the lips. The tongue was not free moving, and the oesophagus was a narrow tube connecting the oropharyngeal cavity to the stomach with no valve or constriction separating it from the later. Histologically, the cavity wall was lined by stratified squamous epithelium containing eosinophilic club cells, mucous cells and taste buds. The laminar propria contained dense collagen fibres. No taste bud was seen on the tongue suggesting it non involvement in food selection through gustation unlike the cavity wall which contained taste buds. The oesophagus was lined by stratified mucous epithelium containing club cells. Histochemistry revealed the presence of neutral, acid and combination of the two. The large extent of oesophageal mucification indicates need for mucin lubrication of food since teleost is known to lack salivary glands and also an adaptation to ingestion of varied feed.La morfología bagre africano del adulto cultivado -Clarias gariepinus- una especie omnívoras cultivables prominente en Nigeria fue investigada. Reveló placas córneas que cubren las superficies vestibulares de los labios. La lengua no tiene movimiento libre y el esófago es un tubo estrecho que conecta la cavidad orofaríngea al estómago sin válvula o constricción que lo separe. Histológicamente, la pared de la cavidad estaba revestida por epitelio escamoso estratificado que contiene células eosinófilas, células mucosas y papilas gustativas. La laminar propia contenía fibras de colágeno denso. No fie vista ninguna papila gustativa en la lengua lo que sugiere la no participación la selección de los alimentos a través gustación a diferencia de la pared de la cavidad que contiene papilas gustativas. El esófago estaba revestido por epitelio estratificado que contiene células mucosas. La histoquímica reveló la presencia de ácido a neutro, y la combinación de los dos. La gran extensión de mucificación esofágica indica la necesidad de lubricación de mucina de los alimentos se sabe que los teleósteos carecen de glándulas salivales y también una adaptación a la ingestión de alimentos variados

Morfología de la cavidad orofaríngea y esófago del bagre africano adulto (<i>Clarias gariepinus</i> Burchell, 1822)

The morphology of the adult farmed African catfish -Clarias gariepinus a prominent omnivorous cultivable species in Nigeria was investigated. It revealed cornified horny plates lining the buccal surfaces of the lips. The tongue was not free moving, and the oesophagus was a narrow tube connecting the oropharyngeal cavity to the stomach with no valve or constriction separating it from the later. Histologically, the cavity wall was lined by stratified squamous epithelium containing eosinophilic club cells, mucous cells and taste buds. The laminar propria contained dense collagen fibres. No taste bud was seen on the tongue suggesting it non involvement in food selection through gustation unlike the cavity wall which contained taste buds. The oesophagus was lined by stratified mucous epithelium containing club cells. Histochemistry revealed the presence of neutral, acid and combination of the two. The large extent of oesophageal mucification indicates need for mucin lubrication of food since teleost is known to lack salivary glands and also an adaptation to ingestion of varied feed.La morfología bagre africano del adulto cultivado -Clarias gariepinus- una especie omnívoras cultivables prominente en Nigeria fue investigada. Reveló placas córneas que cubren las superficies vestibulares de los labios. La lengua no tiene movimiento libre y el esófago es un tubo estrecho que conecta la cavidad orofaríngea al estómago sin válvula o constricción que lo separe. Histológicamente, la pared de la cavidad estaba revestida por epitelio escamoso estratificado que contiene células eosinófilas, células mucosas y papilas gustativas. La laminar propia contenía fibras de colágeno denso. No fie vista ninguna papila gustativa en la lengua lo que sugiere la no participación la selección de los alimentos a través gustación a diferencia de la pared de la cavidad que contiene papilas gustativas. El esófago estaba revestido por epitelio estratificado que contiene células mucosas. La histoquímica reveló la presencia de ácido a neutro, y la combinación de los dos. La gran extensión de mucificación esofágica indica la necesidad de lubricación de mucina de los alimentos se sabe que los teleósteos carecen de glándulas salivales y también una adaptación a la ingestión de alimentos variados.Facultad de Ciencias Veterinaria

Safety of the surgeon: ‘Double-gloving’ during surgical procedures

Background. In the face of increasing HIV/AIDS prevalence in subSaharan Africa, we evaluate the effectiveness of ‘double-gloving’ during surgery as a means of protecting the surgeon operating on patients with a known or unknown HIV status.Methods. A prospective study was conducted to determine the rate of glove puncture and intraoperative injury in categories of patients with known positive, known negative or unknown HIV status.Results. The surgeon and the first assistant double-gloved in all the 1 050 procedures performed between 2009 and 2013, and a total of 8 400 surgical gloves were used. Sixty-nine patients (6.6%) were HIV-positive, 29 patients (2.8%) were HIV-negative, and the HIV status was unknown for the remaining 952 patients (90.7%). The overall glove puncture rate in the study was 14.5%. The glove puncture rate was 0%, 31% and 15% for HIV-positive, HIV-negative and HIV status unknown, respectively, and this difference was statistically significant. The mean operating time in the group with glove punctures was 148 min (95% confidence interval (CI) 135 - 161), while mean operating time in the group without glove puncture was 88 min (95% CI 84 - 92). Conclusion. Double-gloving offers protection against intraoperative injury. Knowing the HIV status of the patient offers additional protection to the operating surgeon. While we recommend routine double-gloving for surgeons working in HIV-prevalent patient populations, we also advocate for the routine screening for HIV in all surgical patients