82 research outputs found
Time variation of bed deformation near bridge piers
CEDR65HWS-YO-SK1.Presented at the International Association for Hydraulic Research, Eleventh International Congress, Leningrad, 1965.Includes bibliographical references.Time variation of scour depth at a circular pier is described in the paper. Adequate design of bridge pier foundation requires proper assessment of this phenomena. The deepest point of scour at any time occurred at the front of the pier, and thus analysis was made for flow in the stagnation plane upstream of the pier. Idealized experimental studies were performed to aid the analysis. Experiments indicated that Froude number was the most important factor in determining scour depth. With known or assumed values of velocity and flow depth, scour dept h can be determined for any time
Scale-up Studies on Immobilization of Lactoperoxidase Using Milk Whey for Producing Antimicrobial Agent
Hypothiocyanite (OSCN–), produced by lactoperoxidase (LPO) in the presence of SCN– and H2O2,inhibits the growth of bacteria. This inhibition is called by LPO system (LPOS). Our laboratory scalestudy in previous experiment showed that whey immobilized on SP-Sepharose Fast Flow (SP-FF) couldproduce OSCN– continuously. Then, the purpose of this study is to scale up continuous production ofOSCN– using immobilized whey. Immobilized whey was generated by circulating various amounts ofwhey through SP-FF. To generate OSCN–, 10 ml of the substrate solution containing 0.5 mM SCN– and0.5 mM H2O2, was circulated through immobilized whey and followed by washing with pure water. Thenext cycle was done by circulating a fresh 10 ml of substrate solution at the same concentration. Theresult indicated that a stable immobilization efficiency of more than 90% was achieved in the SP-FFcirculated with 300 ml or less of whey per gram of SP-FF. When stored at 4˚C, immobilized wheyretained 80% LPO activity until 3 weeks storage. The reaction solution discharged from immobilizedwhey was observed to contain approximately 0.4 mM OSCN–. The experiment using 1.0 g ofimmobilized whey produced a stable 0.4 mM OSCN– production and antimicrobial activity for at least 6cycles. The increase in resin volume accompanied by the increase in whey volume resulted the extensionof a stable OSCN– production. The experiment using recycled SP-FF did not affect to the stability ofOSCN– production and antimicrobial activity. These results may open the way for the large-scaleproduction of OSCN−
Low-field magnetoresistance in GaAs 2D holes
We report low-field magnetotransport data in two-dimensional hole systems in
GaAs/AlGaAs heterostructures and quantum wells, in a large density range, cm, with primary focus on
samples grown on (311)A GaAs substrates. At high densities, cm, we observe a remarkably strong positive magnetoresistance.
It appears in samples with an anisotropic in-plane mobility and predominantly
along the low-mobility direction, and is strongly dependent on the
perpendicular electric field and the resulting spin-orbit interaction induced
spin-subband population difference. A careful examination of the data reveals
that the magnetoresistance must result from a combination of factors including
the presence of two spin-subbands, a corrugated quantum well interface which
leads to the mobility anisotropy, and possibly weak anti-localization. None of
these factors can alone account for the observed positive magnetoresistance. We
also present the evolution of the data with density: the magnitude of the
positive magnetoresistance decreases with decreasing density until, at the
lowest density studied ( cm), it vanishes and is
replaced by a weak negative magnetoresistance.Comment: 8 pages, 8 figure
Performance, feed utilization, and hepatic metabolic response of weaned juvenile Atlantic bluefin tuna (Thunnus thynnus L.): effects of dietary lipid level and source
The development of formulated diets and feeds is essential to increase production of farmed tuna species. There is limited knowledge of this topic, mainly on Pacific Bluefin tuna (Thunnus orientalis) in Japan, whereas no major attempts have been made with Atlantic Bluefin tuna (Thunnus thynnus; ABT). In the present study, two trials were performed using inert formulated diets as on-growing feeds for weaned ABT juvenile in order to establish adequate dietary levels of both lipid and omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). In a first trial, ABT (initial weight = 2.9±0.9g) were fed for 10 days with either a commercial (Magokoro®, MGK) or two experimental feeds with two different lipid levels (15 or 20%) using krill oil (KO) as the single lipid source in order to estimate the suitable lipid content. Fish fed MGK displayed the highest growth, followed by 15KO, with no differences in fish survival. Thus, a lipid content of 15% was considered better than 20% for ABT juveniles. In the second trial, fish (initial weight = 3.3 ± 0.6g) were fed either MGK, 15KO or a feed containing 15% lipid with a combination (1:1, v/v) KO and rapeseed oil (RO) (15KORO). Fish fed 15KO and 15KORO showed the highest growth in terms of weight and fork length (including weight gain and SGR). Increasing dietary lipid level or adding RO to the feeds did not increase liver lipid content. The liver fatty acid profile largely reflected dietary intake confirming very limited LC-PUFA biosynthetic activity for this teleost species. In this respect, liver of fish fed 15KO and 20KO displayed the highest contents of docosahexaenoic acid (DHA). The hepatic expression of genes of lipid and fatty acid metabolism, transcription factors, and antioxidant enzymes was investigated with many of the genes showing regulation by both dietary lipid and LC-PUFA contents. The present study showed promising results that suggested ABT juveniles can be on grown on inert dry feeds that supported good fish growth and the accumulation of the health-promoting fatty acid DHA. Further studies are required in order to fully elucidate lipid and fatty acid requirements of this iconic species regarding dietary sources and production costs.En prensa1,52
SCALE-UP STUDIES ON IMMOBILIZATION OF LACTOPEROXIDASE USING MILK WHEY FOR PRODUCING ANTIMICROBIAL AGENT
Hypothiocyanite (OSCN–), produced by lactoperoxidase (LPO) in the presence of SCN– and H2O2,inhibits the growth of bacteria. This inhibition is called by LPO system (LPOS). Our laboratory scalestudy in previous experiment showed that whey immobilized on SP-Sepharose Fast Flow (SP-FF) couldproduce OSCN– continuously. Then, the purpose of this study is to scale up continuous production ofOSCN– using immobilized whey. Immobilized whey was generated by circulating various amounts ofwhey through SP-FF. To generate OSCN–, 10 ml of the substrate solution containing 0.5 mM SCN– and0.5 mM H2O2, was circulated through immobilized whey and followed by washing with pure water. Thenext cycle was done by circulating a fresh 10 ml of substrate solution at the same concentration. Theresult indicated that a stable immobilization efficiency of more than 90% was achieved in the SP-FFcirculated with 300 ml or less of whey per gram of SP-FF. When stored at 4˚C, immobilized wheyretained 80% LPO activity until 3 weeks storage. The reaction solution discharged from immobilizedwhey was observed to contain approximately 0.4 mM OSCN–. The experiment using 1.0 g ofimmobilized whey produced a stable 0.4 mM OSCN– production and antimicrobial activity for at least 6cycles. The increase in resin volume accompanied by the increase in whey volume resulted the extensionof a stable OSCN– production. The experiment using recycled SP-FF did not affect to the stability ofOSCN– production and antimicrobial activity. These results may open the way for the large-scaleproduction of OSCN−
Nerve fibres containing neuropeptide Y in the atrioventricular valves of Japanese monkey and rat; a light and electron microscopic study
Dense distribution of varicose fibres
containing neuropeptide Y-like immunoreactivity (NPYLI)
was found in the atrioventricular valves of the
Japanese monkey, and moderately in the rat. The
immunoelectron microscopy using immunogolds
resulted in the localization of NPY-LI within the densecored
vesicles which existed with the small clear
vesicles in the unmyelinated axons near the
endocardium. These NPY-LI-containing fibres may
participate in regulation of vasomotor role or other
functions of the atrioventricular valves
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