Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus

Background: A group A Streptococcus (GAS) lineage of genotype emm3, sequence type 15 (ST15) was associated with a six month upsurge in invasive GAS disease in the UK. The epidemic lineage (Lineage C) had lost two typical emm3 prophages, Φ315.1 and Φ315.2 associated with the superantigen ssa, but gained a different prophage (ΦUK-M3.1) associated with a different superantigen, speC and a DNAse spd1. Methods and Results: The presence of speC and spd1 in Lineage C ST15 strains enhanced both in vitro mitogenic and DNAse activities over non-Lineage C ST15 strains. Invasive disease models in Galleria mellonella and SPEC-sensitive transgenic mice, revealed no difference in overall invasiveness of Lineage C ST15 strains compared to non-Lineage C ST15 strains, consistent with clinical and epidemiological analysis. Lineage C strains did however markedly prolong murine nasal infection with enhanced nasal and airborne shedding compared to non-Lineage C strains. Deletion of speC or spd1 in two Lineage C strains identified a possible role for spd1 in airborne shedding from the murine nasopharynx. Conclusions: Nasopharyngeal infection and shedding of Lineage C strains was enhanced compared to nonLineage C strains and this was, in part, mediated by the gain of the DNase spd1 through prophage acquisition

Synthesis of neural networks for spatio-temporal spike pattern recognition and processing

The advent of large scale neural computational platforms has highlighted the lack of algorithms for synthesis of neural structures to perform predefined cognitive tasks. The Neural Engineering Framework offers one such synthesis, but it is most effective for a spike rate representation of neural information, and it requires a large number of neurons to implement simple functions. We describe a neural network synthesis method that generates synaptic connectivity for neurons which process time-encoded neural signals, and which makes very sparse use of neurons. The method allows the user to specify, arbitrarily, neuronal characteristics such as axonal and dendritic delays, and synaptic transfer functions, and then solves for the optimal input-output relationship using computed dendritic weights. The method may be used for batch or online learning and has an extremely fast optimization process. We demonstrate its use in generating a network to recognize speech which is sparsely encoded as spike times.Comment: In submission to Frontiers in Neuromorphic Engineerin

On massive gravitons in 2+1 dimensions

The Fierz-Pauli (FP) free field theory for massive spin 2 particles can be extended, in a spacetime of (1+2) dimensions (3D), to a generally covariant parity-preserving interacting field theory, in at least two ways. One is "new massive gravity" (NMG), with an action that involves curvature-squared terms. Another is 3D "bigravity", which involves non-linear couplings of the FP tensor field to 3D Einstein-Hilbert gravity. We review the proof of the linearized equivalence of both "massive 3D gravity" theories to FP theory, and we comment on their similarities and differences.Comment: 6 pages, to appear in the proceedings of the Spanish Relativity Meeting ERE2009, Bilbao; minor changes, reference adde

Emergence of a novel lineage containing a prophage in emm/M3 group A Streptococcus associated with upsurge in invasive disease in the UK

A sudden increase in invasive Group A Streptococcus (iGAS) infections associated with emm/M3 isolates during the winter of 2008/09 prompted the initiation of enhanced surveillance in England. In order to characterise the population of emm/M3 GAS within the UK and determine bacterial factors that might be responsible for this upsurge, 442 emm/M3 isolates from cases of invasive and non-invasive infections during the period 2001–2013 were subjected to whole genome sequencing. MLST analysis differentiated emm/M3 isolates into three sequence types (STs): ST15, ST315 and ST406. Analysis of the whole genome SNP-based phylogeny showed that the majority of isolates from the 2008–2009 upsurge period belonged to a distinct lineage characterized by the presence of a prophage carrying the speC exotoxin and spd1 DNAase genes but loss of two other prophages considered typical of the emm/M3 lineage. This lineage was significantly associated with the upsurge in iGAS cases and we postulate that the upsurge could be attributed in part to expansion of this novel prophage-containing lineage within the population. The study underlines the importance of prompt genomic analysis of changes in the GAS population, providing an advanced public health warning system for newly emergent, pathogenic strains

Comparison study on reproduction biology of mudskipper (Scartelaos tenuis) from Abi estuarie and Azini estuarie of Hormozgan Provience coastal area

Survey on fecundity of mudskipper Scartelaos tenuis from Abi Estuaries and Azini were carried out from March to April 2010. Data were sampled by hand based on simple random sampling. A totally 219 mudskipper were collected, throughout 113 individual were caught from Abi Estuaries (93 female, 20 male) and 106 invidiual from Azini Estuarie (83 femal, 23 male). The sex ratio of female to male was observed 1:0.24 and it was significantly different from the theoretical 1male:1female sex ratio (P>0.05). Mean total length were calculated 16.32±2.14 cm for female in Bandar Pol and 15.50±2.11 cm for male, and mean length 16.19±2.27 cm for femal and 17.26±1.9 cm for male in Tiab. The result showed no significant differences between mudskipper lengths in two areas (P>0.05). The more frequent length classes were calculated 15-16 cm in Bandar Pol and 18-19 cm in Tiab.The length – weight relationships were estimated as W= 0.0016TL2.7245 for female and W= 0.0138TL2.6523 for male, and the result showed isometric growth. Mean absolute fecundity were estimated 4147±1045 in Bandar pol and 3707±983 in Tiab area, there are no significant differences between fecundity in two areas (P>0.05). The relationship between fecundity-total length were calculated as Fecundity=36.17TL1.6412. The average eggs diameter were calculated 796±144mm in Bandar Pol and 737±137mm in Tiab. The relationship between egg diameter and gonad weight carried out as OD=0.0009GW-0.2167 (R2=0.4728)

Mudskipper species diversity of the Khur-e-Abee protected area, Hormozgan Province

Mudskipper species diversity in the Khur-e-Abee protected area in the coastal areas of the Persian Gulf in Hormozgan Province was studied monthly. Sampling was done from March 2011 to February 2012 is 56º 24' 58” E, 27º 11' 28” N geographical position. A total of 730 specimens belonging to 3 species and 1 family werecollected by hand. Three species were identified; Boleophthalmus dussumieri, Scartelaos tenuis and Periophthalmus waltoni. The monthly sampling of mudskipper species composition showed that S. tenuis (45.75%), P. waltoni (30.48%) and B. dussumieri (26/02%) were first, second and the third in abundance respectively. Shannon species diversity index (1.09), Simpson index (0.62) and Pielou species index (0.99) were highest in October Whereas the Margalef species richness index (0.62) was the highest in December

Covariant coarse-graining of inhomogeneous dust flow in General Relativity

A new definition of coarse-grained quantities describing the dust flow in General Relativity is proposed. It assigns the coarse--grained expansion, shear and vorticity to finite-size comoving domains of fluid in a covariant, coordinate-independent manner. The coarse--grained quantities are all quasi-local functionals, depending only on the geometry of the boundary of the considered domain. They can be thought of as relativistic generalizations of simple volume averages of local quantities in a flat space. The procedure is based on the isometric embedding theorem for S^2 surfaces and thus requires the boundary of the domain in question to have spherical topology and positive scalar curvature. We prove that in the limit of infinitesimally small volume the proposed quantities reproduce the local expansion, shear and vorticity. In case of irrotational flow we derive the time evolution for the coarse-grained quantities and show that its structure is very similar to the evolution equation for their local counterparts. Additional terms appearing in it may serve as a measure of the backreacton of small-scale inhomogeneities of the flow on the large-scale motion of the fluid inside the domain and therefore the result may be interesting in the context of the cosmological backreaction problem. We also consider the application of the proposed coarse-graining procedure to a number of known exact solutions of Einstein equations with dust and show that it yields reasonable results.Comment: 17 pages, 5 figures. Version accepted in Classical and Quantum Gravity

Bragg waveguides with low-index liquid cores

The spectral properties of light confined to low-index media by binary layered structures is discussed. A novel phase-based model with a simple analytical form is derived for the approximation of the center of arbitrary bandgaps of binary layered structures operating at arbitrary effective indices. An analytical approximation to the sensitivity of the bandgap center to changes in the core refractive index is thus derived. Experimentally, significant shifting of the fundamental bandgap of a hollow-core Bragg fiber with a large cladding layer refractive index contrast is demonstrated by filling the core with liquids of various refractive indices. Confirmation of these results against theory is shown, including the new analytical model, highlighting the importance of considering material dispersion. The work demonstrates the broad and sensitive tunability of Bragg structures and includes discussions on refractive index sensing.Kristopher J. Rowland, Shahraam Afshar V., Alexander Stolyarov, Yoel Fink, and Tanya M. Monr

Enhancing the radiation efficiency of dye doped whispering gallery mode microresonators

We present a novel form of a Whispering Gallery Mode (WGM) sensor that exploits dye doped polystyrene microspheres, as active resonators, positioned onto the tip of a Microstructured Optical Fiber (MOF) as a means of overcoming the limited Q-factors for small resonators. We show that it is possible to substantially enhance the fluorescence emission of selected WGMs of the microspheres, resulting in an increase of the signal-to-noise ratio of the modes and of the effective Q-factor. This is done by positioning the resonator into one of the holes of a suspended core MOF and matching the resonator diameter with the hole diameter where it sits, effectively breaking the symmetry of the environment surrounding the sphere. Furthermore we demonstrate that using this experimental configuration, the lasing efficiency of the dye-doped microspheres is also significantly enhanced, which also contributes to an enhancement in the observed Q-factor.Alexandre François, Kristopher J. Rowland, Shahraam Afshar V., Matthew R. Henderson, and Tanya M. Monr